BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0008.p (261 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAC18586.1| siroheme synthase [Paenibacillus macerans] 125 1e-27 ref|NP_954322.1| siroheme synthase, N-terminal domain protein [G... 99 1e-19 ref|ZP_00019187.1| hypothetical protein [Chloroflexus aurantiacus] 93 5e-18 ref|ZP_00181400.1| hypothetical protein Mther048201 [Moorella th... 91 3e-17 ref|ZP_00098848.1| COG1648: Siroheme synthase (precorrin-2 oxida... 87 5e-16 >gb|AAC18586.1| siroheme synthase [Paenibacillus macerans] Length = 218 Score = 125 bits (313), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 70/210 (33%), Positives = 105/210 (50%), Gaps = 2/210 (0%) Query: 47 MVRYLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLS 106 M Y+P+ML+++ +R V++GGG VA RK SP G ++ Sbjct: 1 MPHYMPVMLNLEGRRCVIVGGGTVAARKAAALTEAGAVVTVISPGVTAWLQDRVREGEIA 60 Query: 107 WISRPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVN-VASRSEAGSFITPGVLR 165 W++R Y GD++GAFLV+ LG+PVN A A F P V+R Sbjct: 61 WLAREYREGDLKGAFLVFAATDSRQVNDSIVKEAEMLGIPVNDTADGRRARKFYHPHVVR 120 Query: 166 RGRLTVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIKXXXXXXXXXXXX 225 RG+L + VSTSGAGP+AA + ++ G+ YE Y+++L +RT +K Sbjct: 121 RGKLVIGVSTSGAGPAAARELCREIDRRFGDTYEQYVEFLSLVRTRVKQQVEDKERRKRL 180 Query: 226 XXXVGNLDVLNEMRRGTFIEWSADDIDNWI 255 +G LD+L +R+G F A++I WI Sbjct: 181 LARLGELDILPSIRQGGFTR-GANEIAAWI 209 >ref|NP_954322.1| siroheme synthase, N-terminal domain protein [Geobacter sulfurreducens PCA] gb|AAR36672.1| siroheme synthase, N-terminal domain protein [Geobacter sulfurreducens PCA] Length = 224 Score = 98.6 bits (244), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 56/162 (34%), Positives = 78/162 (48%) Query: 51 LPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWISR 110 LPI ++V + V ++GGG VA RK +P G + R Sbjct: 4 LPISINVSGRLVTIVGGGGVARRKCLSLLEAGARVTVVAPRLDERLMKLADEGAICLAQR 63 Query: 111 PYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRLT 170 PYA GD+ G+ L Y + G+PV+V E GSF +P VLRRG L Sbjct: 64 PYADGDLAGSVLAYAATDDPAVNRAVAEEAHNRGIPVDVTDDPERGSFTSPAVLRRGDLV 123 Query: 171 VAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEI 212 +AVST G P AAR+ + +AEI+G+EY L L +R ++ Sbjct: 124 IAVSTGGGAPGFAARVRDDIAEIIGQEYAETLLILGAIREKL 165 >ref|ZP_00019187.1| hypothetical protein [Chloroflexus aurantiacus] Length = 226 Score = 93.2 bits (230), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 53/164 (32%), Positives = 73/164 (44%) Query: 50 YLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWIS 109 Y P+MLD++D++V+ IGGG E K SP GRL W Sbjct: 2 YYPVMLDLRDKKVLFIGGGIETEVKVQRLVDAGARVRLISPFDHPALDALVQDGRLDWQR 61 Query: 110 RPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRL 169 R Y GD+ G L + R G+ +N FI P + R+G L Sbjct: 62 RAYHYGDLVGFMLCFVHLPDTTLNATIAAEARERGVWLNAVDDPPHCDFILPSIHRQGDL 121 Query: 170 TVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIK 213 +A+STSG P+ A RI E L+ + G EY +LD L R+ +K Sbjct: 122 VIAISTSGVAPALAVRIKEHLSAVYGPEYAAFLDVLRSFRSLVK 165 >ref|ZP_00181400.1| hypothetical protein Mther048201 [Moorella thermoacetica ATCC 39073] Length = 214 Score = 90.9 bits (224), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 49/164 (29%), Positives = 80/164 (48%) Query: 50 YLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLSWIS 109 + P+++D+ D+ +VIGGG V+ERK SP ++ W Sbjct: 3 FYPLVIDLTDRPCLVIGGGTVSERKVLNLLEAGARITLVSPVVTRTLADLALASQVVWWR 62 Query: 110 RPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRRGRL 169 R Y GD++G +V+ G+ VNV E +F P V+RRG L Sbjct: 63 REYREGDLKGMTMVFAATNDPELNARIAVACHQAGILVNVVDNQELCTFNVPAVVRRGDL 122 Query: 170 TVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIK 213 +A+ST+G P+ A ++ +QL +G EY P++D+L ++R +K Sbjct: 123 QIAISTAGKSPALARQLRKQLEAEIGPEYGPWVDFLGEIRPLLK 166 >ref|ZP_00098848.1| COG1648: Siroheme synthase (precorrin-2 oxidase/ferrochelatase domain) [Desulfitobacterium hafniense] Length = 212 Score = 86.7 bits (213), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 52/167 (31%), Positives = 75/167 (44%) Query: 47 MVRYLPIMLDVQDQRVVVIGGGAVAERKXXXXXXXXXXXXXXSPXXXXXXXXXXXXGRLS 106 M Y PI +++Q++ V+V+GGG VA RK SP R Sbjct: 1 MSHYYPIYVELQNKPVLVVGGGTVALRKVKTLLEHGAVVRIVSPELHPELVELVDDERCL 60 Query: 107 WISRPYAPGDIRGAFLVYXXXXXXXXXXXXXXXXRSLGLPVNVASRSEAGSFITPGVLRR 166 W + Y+ D++ LV+ + +NV E +FI P +L R Sbjct: 61 WKKKEYSADDLQDEVLVFSCTEIEEVNSAAAGDAQKSMRLINVVDDPEKCTFIVPSILER 120 Query: 167 GRLTVAVSTSGAGPSAAARITEQLAEILGEEYEPYLDWLHDLRTEIK 213 G L++AVST G+ P A +I +LAE GE YE YL L R ++K Sbjct: 121 GDLSIAVSTGGSSPIVARQIRAELAEHYGEAYEDYLTLLGSWRKDVK 167 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 310,886,053 Number of extensions: 9346525 Number of successful extensions: 24736 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 24703 Number of HSP's successfully gapped: 59 Length of query: 261 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 138 Effective length of database: 423,280,401 Effective search space: 58412695338 Effective search space used: 58412695338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)