BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0175.0016.p (402 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_149213.1| Permease, MDR related [Clostridium acetobutylic... 332 1e-89 ref|NP_906648.1| PUTATIVE MFS TRANSPORTER [Wolinella succinogene... 327 4e-88 gb|EAI73527.1| unknown [environmental sequence] 320 3e-86 ref|NP_746761.1| membrane protein, putative [Pseudomonas putida ... 312 8e-84 ref|NP_522738.1| PROBABLE TRANSMEMBRANE PROTEIN [Ralstonia solan... 310 5e-83 >ref|NP_149213.1| Permease, MDR related [Clostridium acetobutylicum] gb|AAK76795.1| Permease, MDR related [Clostridium acetobutylicum] Length = 408 Score = 332 bits (851), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 181/390 (46%), Positives = 245/390 (62%), Gaps = 2/390 (0%) Query: 13 ARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXXXXXXXXXXXX 72 AR+ S A+QML+VAIGWQMY+LT SAF LGL+GL QF Sbjct: 21 ARVSSNFAYQMLTVAIGWQMYSLTHSAFYLGLIGLVQFLPMLVLSLFVGHISDRYDRRKI 80 Query: 73 XXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAALVPDIVDREQ 132 + QL++ G+ GW +FEGP AL+P+IV+R+ Sbjct: 81 VS-ISQLVQGICIALLAFGNYNGWITKESFLIMVFFIALAHSFEGPPMQALLPNIVERKI 139 Query: 133 LPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIFFVKTVHFVKK 192 PK Q A+IVGP G+L +G + Y + G L++++VLI + +K Sbjct: 140 FPKASALVSSASQFAVIVGPGFAGILYSFGASLIYSITAGLLILASVLIIQIHVNKEERK 199 Query: 193 LDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDILKTGSLGLGLL 252 D VN+ +F +G+ F+ +R IILG ISLDLFAVL GGATALLPI+A ILK GS GLG+L Sbjct: 200 PDPVNLKSFFAGITFIKSRPIILGAISLDLFAVLFGGATALLPIYASSILKIGSFGLGVL 259 Query: 253 RSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWLSLFALFLIGA 312 RSAPAVGA+++S +LTR ++R +GK +F ++V F L+TI+F +S S +S ALF++GA Sbjct: 260 RSAPAVGALLMSALLTRKPLKRNVGKIMFTAVVCFGLSTIIFAISKSVIVSFLALFVLGA 319 Query: 313 SDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGLVGAVPATVIG 372 SDV+SVVIRSTLVQ+ TP M+GRV++VN +FIGTSNQLGEFESG A L+G VPA ++G Sbjct: 320 SDVISVVIRSTLVQLETPDNMRGRVSSVNMIFIGTSNQLGEFESGITASLLGTVPAALLG 379 Query: 373 GLGTLVISFIWMYWLFPVLRTQKTYTAEKG 402 G+GT+++ IW LF L T ++ K Sbjct: 380 GVGTILVVLIWRK-LFSSLFTVDSFEGSKN 408 >ref|NP_906648.1| PUTATIVE MFS TRANSPORTER [Wolinella succinogenes] emb|CAE09548.1| PUTATIVE MFS TRANSPORTER [Wolinella succinogenes] Length = 415 Score = 327 bits (837), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 176/400 (44%), Positives = 254/400 (63%), Gaps = 3/400 (0%) Query: 3 HNRSFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXX 62 F R+ AR S +QMLSVA+GWQ+Y+LT F LGLVGLAQF Sbjct: 9 EKLDFWRILSARALSMFCYQMLSVAVGWQVYSLTGEVFDLGLVGLAQFLPMFLLTLLVGQ 68 Query: 63 XXXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAA 122 L Q +++ GS+ GW R FEGP+ A Sbjct: 69 VADWYDRRVIVA-LAQALQAMGVLLLAWGSLGGWLDLYHILGILLLLSSVRAFEGPSIQA 127 Query: 123 LVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIF 182 L+P++V +E PK Q+A+I+GP+LGGVL VWG + Y ++V+++L+ Sbjct: 128 LLPNLVSKEFFPKAVAWMASCTQSAVIIGPALGGVLYVWGAQSVYGTVSALMVVASLLVV 187 Query: 183 FVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDIL 242 V+ H + ++ ++ L+G+ F+ +R ++G ISLDLFAVLLGGATA+LP++A+DIL Sbjct: 188 GVRRTHEIASRQKMSKESLLAGIAFIRSRPAMMGAISLDLFAVLLGGATAMLPVYAKDIL 247 Query: 243 KTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWL 302 +T ++GLGLLR+APA+GA++VSL L R+ ++ +G+ +F ++++F LATI F +S FWL Sbjct: 248 ETDTVGLGLLRAAPAIGALLVSLYLARFPLQGGVGRKMFRAVMIFGLATIGFALSTHFWL 307 Query: 303 SLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGL 362 S+ L ++G SDVVSVVIRS+LVQ++TP EM+GRV+AVNS+FIG SNQLGEFESG A L Sbjct: 308 SMGMLVVLGGSDVVSVVIRSSLVQLSTPDEMRGRVSAVNSMFIGASNQLGEFESGMAAAL 367 Query: 363 VGAVPATVIGGLGTLVISFIWMYWLFPVL-RTQKTYTAEK 401 +G VP+ V+GG+GT++++ WM LFP L R ++ E Sbjct: 368 LGVVPSVVLGGVGTILVALWWMR-LFPELSRIERLKDLEP 406 >gb|EAI73527.1| unknown [environmental sequence] Length = 420 Score = 320 bits (821), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 176/399 (44%), Positives = 237/399 (59%), Gaps = 2/399 (0%) Query: 4 NRSFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXXX 63 + F R W RI S+ +FQML+VA+GW +YALT SAF LGLVGLAQF Sbjct: 19 HPPFQRFWCTRILSSLSFQMLAVAMGWHIYALTHSAFALGLVGLAQFLPMFVLTLVVGHV 78 Query: 64 XXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAAL 123 +CQ +ES G+ GW R FE PA A+L Sbjct: 79 ADRYDRRRIAA-ICQSLESVAALLFAVGTFGGWISAPVIYVLAACVGAARAFESPAVASL 137 Query: 124 VPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIFF 183 +P +V R QLPK Q+A I GP+LGG+L G AAY+A + + ++ Sbjct: 138 LPGVVPRAQLPKATAWATSANQSAQIAGPALGGLLYGIGPGAAYLACTLSFAAAAASVWA 197 Query: 184 VKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDILK 243 + + V +++ SG+ F+ +ILG +SLDLFAVL GGATALLP+FA DIL Sbjct: 198 IPLQAKLASRAPVTLESVFSGIAFIRKEPVILGALSLDLFAVLFGGATALLPVFARDILH 257 Query: 244 TGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWLS 303 TG LGLGLLRS A+GA+ ++ L + + G +F ++ F ATI+FG+SH F+LS Sbjct: 258 TGPLGLGLLRSGTAIGALAGTIWLAHFPLRNRPGAAMFGGVIAFGAATIVFGLSHQFFLS 317 Query: 304 LFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGLV 363 FAL ++GASD +SVV+R +LVQ+ TP EM GRV+AVNSLFIGTSNQLGEFESG AG Sbjct: 318 FFALMVLGASDTISVVVRLSLVQLRTPDEMLGRVSAVNSLFIGTSNQLGEFESGVTAGWW 377 Query: 364 GAVPATVIGGLGTLVISFIWMYWLFPVLRTQKTYTAEKG 402 GA PA ++GG+ T+ ++ +WM + FP L+ ++ E+G Sbjct: 378 GAQPAVLVGGIATIAVALLWMRF-FPELKRTRSLEREEG 415 >ref|NP_746761.1| membrane protein, putative [Pseudomonas putida KT2440] gb|AAN70225.1| membrane protein, putative [Pseudomonas putida KT2440] Length = 406 Score = 312 bits (800), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 175/393 (44%), Positives = 243/393 (61%), Gaps = 2/393 (0%) Query: 3 HNRSFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXX 62 H+R FL W+AR+F+ S FQML+VAIGW +Y LT + LGLVGL +F Sbjct: 11 HHRPFLAFWLARVFTASGFQMLTVAIGWHLYQLTGNVLDLGLVGLVEFAPRVLFMLHTGH 70 Query: 63 XXXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAA 122 LCQ ++ GS R+FE PA+ A Sbjct: 71 VADRYDRRKVAA-LCQSLQGLIALALAVGSATDNVTRELIFALAFLLGASRSFEMPATQA 129 Query: 123 LVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIF 182 L+P++V P+ Q+A IV P++GG L +G+ Y ++ ++ VL Sbjct: 130 LLPNVVPAGLFPRAVAASASATQSATIVAPAVGGFLYAFGSMWVYGPTVALYAIACVLTL 189 Query: 183 FVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDIL 242 ++ V + ++++ L+G+RF+ +R ILG ISLDLFAVLLGGATALLP+FA+DIL Sbjct: 190 SLQARGQVAQRGRASIESLLAGIRFIRSRPDILGAISLDLFAVLLGGATALLPVFAKDIL 249 Query: 243 KTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWL 302 TG +GLGLLRSAPAVGA+++SL L R+ +R +G+T+F ++ VF +ATI FG+S SFW Sbjct: 250 LTGPMGLGLLRSAPAVGALLMSLWLARFPFQRNVGRTMFTAVGVFGVATIAFGLSTSFWF 309 Query: 303 SLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGL 362 SL L ++GA+D++S+VIRS+ VQ+ TP EM+GRV+AVN LFIG SNQLGEFESG A Sbjct: 310 SLAVLVVLGAADMISMVIRSSFVQLETPDEMRGRVSAVNGLFIGASNQLGEFESGVTAHW 369 Query: 363 VGAVPATVIGGLGTLVISFIWMYWLFPVLRTQK 395 G VPA V+GG+GTLV++ +W+ LFP L + Sbjct: 370 FGTVPAVVLGGVGTLVVTGVWIK-LFPTLAKRD 401 >ref|NP_522738.1| PROBABLE TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] emb|CAD18328.1| PROBABLE TRANSMEMBRANE PROTEIN [Ralstonia solanacearum] Length = 424 Score = 310 bits (793), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 173/401 (43%), Positives = 248/401 (61%), Gaps = 6/401 (1%) Query: 6 SFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXXXXX 65 +F W+AR+ +T A+QM VA+GWQ+Y LT SA+ LGLVGLAQF Sbjct: 26 AFRLFWLARLSTTFAYQMFGVAVGWQIYDLTRSAYVLGLVGLAQFLPSVVLVLASGHIAD 85 Query: 66 XXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAALVP 125 +CQ +E+ ++ G R FE P AL+P Sbjct: 86 RYDRRRVVR-ICQAIEALVAAMLAAATLTGHASIQPIFVFVVVIGAMRAFETPTLQALLP 144 Query: 126 DIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIFFVK 185 +V LP+ QT +I+GP++GG L V G Y + G +++ VL+ V+ Sbjct: 145 SLVPPAALPRAVALSSSAGQTGIILGPAVGGFLYVAGAPVVYATAAGLFVLAHVLVACVR 204 Query: 186 TVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDILKTG 245 T V++++ +G+ F+ R ++LG ISLDLFAVLLGGATALLPI+A DIL TG Sbjct: 205 TGRAAPVSRPVSLESLFAGIAFIKGRPVVLGAISLDLFAVLLGGATALLPIYARDILGTG 264 Query: 246 SLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWLSLF 305 + GLGLLRSAPA+GA+ +++ L +ER +G+ +FA++ +F LAT++FGVS LS+ Sbjct: 265 AWGLGLLRSAPAIGALAMAIFLAHRPLERHVGRVMFAAVALFGLATMVFGVSRWLPLSML 324 Query: 306 ALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGLVGA 365 AL ++GA D+VSVVIR++LVQ++TP M+GRV+AVNS+F+G SNQLGEFESG +AG++G Sbjct: 325 ALVVLGAGDMVSVVIRTSLVQLDTPDAMRGRVSAVNSVFVGASNQLGEFESGMVAGMLGP 384 Query: 366 VPATVIGGLGTLVISFIWMYWLFPVL----RTQKTYTAEKG 402 VP+ V+GGLGTL++ +WM LFP L R ++ + A +G Sbjct: 385 VPSVVLGGLGTLLVVALWMR-LFPALTHRDRLREAHPAGEG 424 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 441,898,718 Number of extensions: 15948587 Number of successful extensions: 63840 Number of sequences better than 1.0e-05: 267 Number of HSP's gapped: 63796 Number of HSP's successfully gapped: 271 Length of query: 402 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 274 Effective length of database: 409,704,906 Effective search space: 112259144244 Effective search space used: 112259144244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)