BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0173.0003.p (453 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244897.1| replicative DNA helicase [Bacillus halodurans] ... 638 0.0 ref|NP_653935.1| DnaB, DnaB-like helicase [Bacillus anthracis A2... 617 e-175 gb|AAD20314.1| replicative helicase; DnaB [Geobacillus stearothe... 609 e-173 ref|NP_391924.1| replicative DNA helicase [Bacillus subtilis] >g... 607 e-172 prf||2112338A DNA helicase 596 e-169 >ref|NP_244897.1| replicative DNA helicase [Bacillus halodurans] pir||E84153 replicative DNA helicase dnaC [imported] - Bacillus halodurans (strain C-125) dbj|BAB07748.1| replicative DNA helicase [Bacillus halodurans] Length = 454 Score = 638 bits (1645), Expect = 0.0, Method: Compositional matrix adjust. Identities = 300/446 (67%), Positives = 370/446 (82%), Gaps = 1/446 (0%) Query: 4 DLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEES 63 DLF DR PPQN+EAEQAV+GA+ L+D AL+TA ER+ EDFY HQ I++ M+ L E+ Sbjct: 3 DLFADRTPPQNIEAEQAVLGAIFLEDHALVTASERLAPEDFYRAAHQRIYQVMLDLAEKG 62 Query: 64 QPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTA 123 +P+DLVT+T+ LQD+ L+D+GGV+YL LA+AVPTAANV+YY++I+EEK++LRRLIR A Sbjct: 63 EPVDLVTVTAELQDRKALDDVGGVAYLGDLANAVPTAANVEYYSKIVEEKSLLRRLIRVA 122 Query: 124 TQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGG 183 T I EGY E+V +L DAE+ IL+++ R++ S FI+I+DVL++ +D++E+L KG Sbjct: 123 TNIAQEGYASEEEVDAILDDAEKTILDVAQRKNSSAFISIKDVLVETYDKIEMLQNQKGE 182 Query: 184 TSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEM 243 +GIPTGF DLD MT GFQRNDLIIVAARPSVGKTAFALNI+QNVA + E VAIFSLEM Sbjct: 183 ITGIPTGFKDLDRMTAGFQRNDLIIVAARPSVGKTAFALNISQNVATKTDENVAIFSLEM 242 Query: 244 SAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDI 303 A QLVQRM+CAE N+DA MRTG +++DW KLTM + SL+ + IYIDDTPG+ V +I Sbjct: 243 GASQLVQRMLCAEGNIDAQRMRTGAL-TEEDWQKLTMAMGSLARAGIYIDDTPGIKVNEI 301 Query: 304 RAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALS 363 RAKCRRLK+EKGLGMI+IDYLQLIQG G++GENRQQEVSEISR+LK +AREL+VPVIALS Sbjct: 302 RAKCRRLKQEKGLGMILIDYLQLIQGNGRSGENRQQEVSEISRSLKGLARELEVPVIALS 361 Query: 364 QLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRN 423 QLSRGVE RQDKRPMMSD+RESGSIEQDADIVAFLYRDDYY+++TE +N AKQRN Sbjct: 362 QLSRGVESRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYDKETENQNIIEIIIAKQRN 421 Query: 424 GPVGTVELVFLKNFNKFVNYERAHNE 449 GPVGTVEL F+K +NKFVN +R H+E Sbjct: 422 GPVGTVELAFIKEYNKFVNLDRHHDE 447 >ref|NP_653935.1| DnaB, DnaB-like helicase [Bacillus anthracis A2012] ref|NP_847863.1| replicative DNA helicase [Bacillus anthracis str. Ames] gb|AAP29349.1| replicative DNA helicase [Bacillus anthracis str. Ames] Length = 453 Score = 617 bits (1591), Expect = e-175, Method: Compositional matrix adjust. Identities = 299/442 (67%), Positives = 360/442 (81%), Gaps = 1/442 (0%) Query: 4 DLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEES 63 D+ DR PP N+EAEQAV+GA+L+ +AL +A E + + FY HQ IFE M+ L ++ Sbjct: 3 DVMADRTPPHNIEAEQAVLGAILIDQDALTSASELLVPDSFYRTKHQKIFEVMLGLSDKG 62 Query: 64 QPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTA 123 +PIDLV +TS + D+G LE++GGVSYLA+LA VPTAANV+YYA+II EKA+LRRLIRTA Sbjct: 63 EPIDLVMMTSAMADQGLLEEVGGVSYLAELAEVVPTAANVEYYARIIAEKALLRRLIRTA 122 Query: 124 TQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGG 183 T IVS+GY +DV +L++AE++ILE+S++ + F I+DVL+ +D++ELLH KG Sbjct: 123 THIVSDGYEREDDVDGLLNEAEKKILEVSHQTNAKAFQNIKDVLVDAYDKIELLHNQKGE 182 Query: 184 TSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEM 243 +GIPTGF +LD MT GFQRNDLIIVAARPSVGKTAF+LNIAQNVA + E VAIFSLEM Sbjct: 183 VTGIPTGFTELDKMTAGFQRNDLIIVAARPSVGKTAFSLNIAQNVATKTDENVAIFSLEM 242 Query: 244 SAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDI 303 A QLV RM+CAE N+DA +RTG S DDW+KLTM + SLS + IYIDDTPG+ V +I Sbjct: 243 GADQLVMRMLCAEGNIDAQRLRTGSLTS-DDWAKLTMAMGSLSNAGIYIDDTPGIKVNEI 301 Query: 304 RAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALS 363 RAKCRRLK+E+GLGMI+IDYLQLIQG GK+GENRQQEVSEISRTLK IAREL VPVIALS Sbjct: 302 RAKCRRLKQEQGLGMILIDYLQLIQGSGKSGENRQQEVSEISRTLKGIARELQVPVIALS 361 Query: 364 QLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRN 423 QLSRGVE RQDKRPMMSD+RESGSIEQDADIVAFLYR+DYY+++TE KN AKQRN Sbjct: 362 QLSRGVESRQDKRPMMSDIRESGSIEQDADIVAFLYREDYYDRETENKNTIEIIIAKQRN 421 Query: 424 GPVGTVELVFLKNFNKFVNYER 445 GPVG+VEL F+K FNKFVN ER Sbjct: 422 GPVGSVELAFVKEFNKFVNLER 443 >gb|AAD20314.1| replicative helicase; DnaB [Geobacillus stearothermophilus] Length = 454 Score = 609 bits (1571), Expect = e-173, Method: Compositional matrix adjust. Identities = 293/447 (65%), Positives = 361/447 (80%), Gaps = 1/447 (0%) Query: 3 GDLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEE 62 +LF +RIPPQ++EAEQAV+GAV L AL+ A E + EDFY HQ IF AM+++ + Sbjct: 2 SELFSERIPPQSIEAEQAVLGAVFLDPAALVPASEILIPEDFYRAAHQKIFHAMLRVADR 61 Query: 63 SQPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRT 122 +P+DLVT+T+ L QLE+IGGVSYL++LA AVPTAANV+YYA+I+EEK++LRRLIRT Sbjct: 62 GEPVDLVTVTAELAASEQLEEIGGVSYLSELADAVPTAANVEYYARIVEEKSVLRRLIRT 121 Query: 123 ATQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKG 182 AT I +GYT +++ +L +A+R+I+E+S R+ F I+D+L+Q +D +E+LH G Sbjct: 122 ATSIAQDGYTREDEIDVLLDEADRKIMEVSQRKHSGAFKNIKDILVQTYDNIEMLHNRDG 181 Query: 183 GTSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLE 242 +GIPTGF +LD MT+GFQR+DLIIVAARPSVGKTAFALNIAQNVA + E VAIFSLE Sbjct: 182 EITGIPTGFTELDRMTSGFQRSDLIIVAARPSVGKTAFALNIAQNVATKTNENVAIFSLE 241 Query: 243 MSAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTD 302 MSA QLV RM+CAE N++A +RTG + +DW KLTM + SLS + IYIDDTP + V+D Sbjct: 242 MSAQQLVMRMLCAEGNINAQNLRTGKL-TPEDWGKLTMAMGSLSNAGIYIDDTPSIRVSD 300 Query: 303 IRAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIAL 362 IRAKCRRLK+E GLGMIVIDYLQLIQG G++ ENRQQEVSEISR+LK +AREL+VPVIAL Sbjct: 301 IRAKCRRLKQESGLGMIVIDYLQLIQGSGRSKENRQQEVSEISRSLKALARELEVPVIAL 360 Query: 363 SQLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQR 422 SQLSR VEQRQDKRPMMSD+RESGSIEQDADIVAFLYRDDYYN+D+E KN AKQR Sbjct: 361 SQLSRSVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYNKDSENKNIIEIIIAKQR 420 Query: 423 NGPVGTVELVFLKNFNKFVNYERAHNE 449 NGPVGTV+L F+K +NKFVN ER +E Sbjct: 421 NGPVGTVQLAFIKEYNKFVNLERRFDE 447 >ref|NP_391924.1| replicative DNA helicase [Bacillus subtilis] sp|P37469|DNAC_BACSU Replicative DNA helicase pir||S65970 replicative DNA helicase dnaC - Bacillus subtilis dbj|BAA05176.1| replicative DNA helicase [Bacillus subtilis] emb|CAB16081.1| replicative DNA helicase [Bacillus subtilis subsp. subtilis str. 168] Length = 454 Score = 607 bits (1564), Expect = e-172, Method: Compositional matrix adjust. Identities = 291/442 (65%), Positives = 360/442 (81%), Gaps = 1/442 (0%) Query: 4 DLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEES 63 DL DR+PPQN+EAEQAV+GA+ LQ AL A E + +DFY HQ I+ AM+ LG+ Sbjct: 3 DLLNDRLPPQNIEAEQAVLGAIFLQPSALTLASEVLIPDDFYRMSHQKIYNAMLVLGDRG 62 Query: 64 QPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTA 123 +P+DLVT+TS L + LE++GG+SYL +A++VPTAAN++YYA+I+EEK++LRRLIRTA Sbjct: 63 EPVDLVTVTSELANTDLLEEVGGISYLTDIANSVPTAANIEYYAKIVEEKSILRRLIRTA 122 Query: 124 TQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGG 183 T I +GYT ++V ++LS+AE+ I+E++ R++ S F I+DVL+Q +D +E L+ KG Sbjct: 123 TTIAQDGYTREDEVEDLLSEAEKTIMEVAQRKNTSAFQNIKDVLVQTYDNIEQLYNRKGD 182 Query: 184 TSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEM 243 +GIPTGF +LD MT GFQRNDLIIVAARPSVGKTAFALNIAQNVA + E+VAIFSLEM Sbjct: 183 ITGIPTGFTELDRMTAGFQRNDLIIVAARPSVGKTAFALNIAQNVATKTDESVAIFSLEM 242 Query: 244 SAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDI 303 A QLV RM+CAE N++A +RTG+ +++DW KLTM + SLS S IYIDDTPG+ V++I Sbjct: 243 GAEQLVMRMLCAEGNINAQNLRTGNL-TEEDWGKLTMAMGSLSNSGIYIDDTPGIRVSEI 301 Query: 304 RAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALS 363 RAKCRRLK+E GLGMI+IDYLQLIQG G++ +NRQQEVSEISR LK IAREL VPVIALS Sbjct: 302 RAKCRRLKQESGLGMILIDYLQLIQGSGRSKDNRQQEVSEISRELKSIARELQVPVIALS 361 Query: 364 QLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRN 423 QLSRGVEQRQDKRPMMSD+RESGSIEQDADIVAFLYRDDYY+++TE KN AKQRN Sbjct: 362 QLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYDKETENKNIIEIIIAKQRN 421 Query: 424 GPVGTVELVFLKNFNKFVNYER 445 GPVGTV L F+K +NKFVN ER Sbjct: 422 GPVGTVSLAFVKEYNKFVNLER 443 >prf||2112338A DNA helicase Length = 454 Score = 596 bits (1537), Expect = e-169, Method: Compositional matrix adjust. Identities = 287/442 (64%), Positives = 357/442 (80%), Gaps = 1/442 (0%) Query: 4 DLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEES 63 DL DR+PPQN+EAEQAV+GA+ LQ AL A E + +DFY HQ I+ AM+ LG+ Sbjct: 3 DLLNDRLPPQNIEAEQAVLGAIFLQPSALTLASEVLIPDDFYRMSHQKIYNAMLVLGDRG 62 Query: 64 QPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTA 123 +P+DLVT+TS L + LE++GG+SYL +A++VPTAAN++YYA+I+EEK++LRRLIRTA Sbjct: 63 EPVDLVTVTSELANTDLLEEVGGISYLTDIANSVPTAANIEYYAKIVEEKSILRRLIRTA 122 Query: 124 TQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGG 183 T I +GYT ++V ++LS+AE+ I+E++ R++ S F I+DVL+Q +D +E L+ KG Sbjct: 123 TTIAQDGYTREDEVEDLLSEAEKTIMEVAQRKNTSAFQNIKDVLVQTYDNIEQLYNRKGD 182 Query: 184 TSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEM 243 +GIPTGF +LD MT GFQRNDLIIVAARPS+GKTAFALNIAQNVA + +VAIFSL M Sbjct: 183 ITGIPTGFTELDRMTAGFQRNDLIIVAARPSMGKTAFALNIAQNVATKTDFSVAIFSLFM 242 Query: 244 SAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDI 303 A QLV RM+CAE N++A +RTG+ +++DW KLTM + SLS S IYIDD PG+ V++I Sbjct: 243 GAEQLVMRMLCAEGNINAQNLRTGNL-TEEDWGKLTMAMGSLSNSGIYIDDIPGIRVSEI 301 Query: 304 RAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALS 363 RAKCRRLK+E GLGMI+IDYLQLIQG G++ +NRQQEVSEISR LK IAREL VPVIALS Sbjct: 302 RAKCRRLKQESGLGMILIDYLQLIQGSGRSKDNRQQEVSEISRELKSIARELQVPVIALS 361 Query: 364 QLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRN 423 QLSRGVEQRQDKRPMMSD+RESGSIEQDADIVAFLYRDDYY+++TE KN AKQRN Sbjct: 362 QLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYDKETENKNIIEIIIAKQRN 421 Query: 424 GPVGTVELVFLKNFNKFVNYER 445 GPVGTV L F+K +NKFVN ER Sbjct: 422 GPVGTVSLAFVKEYNKFVNLER 443 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 614,137,531 Number of extensions: 24136907 Number of successful extensions: 79496 Number of sequences better than 1.0e-05: 927 Number of HSP's gapped: 77316 Number of HSP's successfully gapped: 941 Length of query: 453 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 324 Effective length of database: 406,989,807 Effective search space: 131864697468 Effective search space used: 131864697468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)