BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0007.p (449 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S63614 malE protein homolog cymE precursor - Klebsiella oxy... 218 3e-55 dbj|BAB40635.1| periplasmic binding protein [Thermoactinomyces v... 216 7e-55 ref|NP_391296.1| similar to maltose/maltodextrin-binding protein... 207 3e-52 ref|NP_404477.1| putative sugar-binding protein [Yersinia pestis... 197 6e-49 ref|NP_980358.1| maltosaccharide ABC transporter, maltosaccharid... 193 6e-48 >pir||S63614 malE protein homolog cymE precursor - Klebsiella oxytoca emb|CAA60004.1| cymE [Klebsiella oxytoca] Length = 409 Score = 218 bits (555), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 135/382 (35%), Positives = 202/382 (52%), Gaps = 12/382 (3%) Query: 70 MEPEQGAKLMVWEAKEQLEYMKGVAADFEKKYG--VPVNVEELAGGDQGGRLATDGPAKL 127 + PE+ A L++W L+YMK A +F K Y V LA D R+ DG + Sbjct: 26 IAPEKQANLLIWTDISTLDYMKYAAENFNKTYNSQVRFTFRGLAPIDAASRMIQDGGSTR 85 Query: 128 AADVLTLPHDQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYA 187 ADV + HD + + V AG ++ N + E + A+ AS Y+G YG+P S T A Sbjct: 86 VADVAEIEHDLLGRLVVAGGVMENLISAERINKKFMSNAINASRYEGNNYGFPVSYATVA 145 Query: 188 LFYNKDLVPEAPKTWNDIIAFANTYNKPKENKFAIMWEFVGYY-TYPFIGSFGGYIFGDS 246 LFYNKD++ P + ++I + +N KENK+ ++W+ YY + F+ +G Y FG Sbjct: 146 LFYNKDILRLPPDKFEELIQLSAEFNNVKENKYTLLWDVQNYYESRMFLTLYGAYEFGQE 205 Query: 247 NSNPKDIGLNNEGTIKGFTFLQSLKKILPLNAGDITF-DVKTQLFQEGKLAMNIDGPWST 305 +N KD+G+N+E KG ++ LK P N D+ V+ LF EGK+A IDGPW+ Sbjct: 206 GTNVKDLGINSELAQKGLAAMKRLKTANPSNPVDMRNPQVRRGLFSEGKVAAIIDGPWAI 265 Query: 306 GSFKD-HVNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLK 364 + + +NFGVVP+P G++ SFS V+ V+SY+ P AA+LFA + SS + +K Sbjct: 266 QGYNNSKINFGVVPIPT-LEGKQPRSFSTVRLAVVSSYSKSPKAAQLFADYLSSDEMLMK 324 Query: 365 NYKMTGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAFVSLW 424 Y+MT +IP + T +Q HS MP+I E+ +W PL A +W Sbjct: 325 RYEMTESIPPVPNLMNKILPTANEATSAIIKQGLHSDAMPSIPEMGYLWSPLANAITDMW 384 Query: 425 -ND----PGLD-VKQTMDQTVK 440 ND LD + +D+ +K Sbjct: 385 INDQTPKAALDRARNIIDEQIK 406 >dbj|BAB40635.1| periplasmic binding protein [Thermoactinomyces vulgaris] Length = 417 Score = 216 bits (551), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 142/411 (34%), Positives = 216/411 (52%), Gaps = 34/411 (8%) Query: 18 MNAVSKIVVLCMLLVMTATACSSNSPNTAPSPKGGNNQTAETSAPENTADPEMEPEQGAK 77 M + ++ L++ TAC P P K G + E +P+ K Sbjct: 1 MRKFLSLALISALVIGLLTACG---PKRDPYAKAGKS--------------EGKPD---K 40 Query: 78 LMVWEAKE---QLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTL 134 L+VWE + QL K A +F KK G+ V V +A Q +L DGPA AD++T Sbjct: 41 LVVWENADDGVQLNNTKKWAGEFTKKTGIQVEVVPVALLKQQEKLTLDGPAGKGADLVTW 100 Query: 135 PHDQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDL 194 PHD++ +AV GLL P V + K + A++A +Y G LYG PK++E+ AL YNK L Sbjct: 101 PHDRLGEAVTKGLLQPIQV-DNSVKNQFDDVAMKALTYGGKLYGLPKAIESVALIYNKKL 159 Query: 195 VPEAPKTWNDIIAFANTYNKPKENKFAIMWEFVG-YYTYPFIGSFGGYIFG--DSNSNPK 251 + + P T++++ +A NKP E K+ +++E YYTY + G +F D +P Sbjct: 160 MGQVPATYDELFQYAKANNKPDEQKYGVLFEANNFYYTYFLFAAKGAAVFKEQDGTLDPN 219 Query: 252 DIGLNNEGTIKGFTFLQS--LKKILPLNAGDITFDVKTQLFQEGKLAMNIDGPWSTGSFK 309 +IGLN+ ++G +Q + LP + + D LF+ GK+A I+GPW+ ++ Sbjct: 220 EIGLNSPEAVQGMNEVQKWFTEARLPQS---LKADTVNGLFKSGKVAAVINGPWAIKDYQ 276 Query: 310 DH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYKM 368 +N GV PLPK +G++ +F GVK +Y+++Y+ YP A F +SK+ +K Sbjct: 277 AAGINVGVAPLPK-IDGKDAQTFIGVKGWYLSAYSKYPKYATELMQFLTSKEALASRFKE 335 Query: 369 TGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAA 419 TG IP E IKN+P+ GF +Q VPMP+I E+ VWEP+ A Sbjct: 336 TGEIPPQKELLNDPMIKNNPVVNGFAKQASKGVPMPSIPEMGVVWEPINNA 386 >ref|NP_391296.1| similar to maltose/maltodextrin-binding protein [Bacillus subtilis] pir||C70038 maltose/maltodextrin-binding protein homolog yvfK - Bacillus subtilis emb|CAB08005.1| hypothetical protein [Bacillus subtilis] emb|CAB15421.1| yvfK [Bacillus subtilis subsp. subtilis str. 168] Length = 421 Score = 207 bits (528), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 136/382 (35%), Positives = 200/382 (52%), Gaps = 12/382 (3%) Query: 77 KLMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPH 136 +L+VWE KE+ +K A FEK++ V V V E Q L DGPA DVLT+P Sbjct: 39 ELVVWEDKEKSNGIKDAVAAFEKEHDVKVKVVEKPYAKQIEDLRMDGPAGTGPDVLTMPG 98 Query: 137 DQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVP 196 DQI AV GLL V +++ ++ + ++Q+ D LYG PK+VET LFYNKDL+ Sbjct: 99 DQIGTAVTEGLLKELHV-KKDVQSLYTDASIQSQMVDQKLYGLPKAVETTVLFYNKDLIT 157 Query: 197 EA--PKTWNDIIAFANTYNKPKENKFAIMWEFVGYYTYPFIGSFGGYIFG---DSNSNPK 251 E PKT + ++ + F +++ + YY + +GGYIFG D + NP Sbjct: 158 EKELPKTLEEWYDYSKKTADGSKFGFLALFDQI-YYAESVMSGYGGYIFGKAKDGSYNPS 216 Query: 252 DIGLNNEGTIKGFTFLQSLKK--ILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWSTGSF 308 DIG+NNEG +KG +Q K + P G+ +V LF EGK A I GPW+ +F Sbjct: 217 DIGINNEGAVKGAALIQKFYKDGLFPAGIIGEQGINVLESLFTEGKAAAIISGPWNVEAF 276 Query: 309 KDH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYK 367 + +N+G+ LPK NG+ M SF GVKSY V++++ A+ A F +++ N Y+ Sbjct: 277 SNAGINYGITKLPKLENGKNMSSFIGVKSYNVSAFSKNEELAQELAVFLANEKNSKTRYE 336 Query: 368 MTGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAFVSLWNDP 427 T +PA I EQ + S P P I E+N +W P +A ++ Sbjct: 337 ETKEVPAVKSLANDPAIMKSEAARAVTEQSRFSEPTPNIPEMNEIWTPADSALQTVATGK 396 Query: 428 GLDVKQTMDQTVKTIQSDLESK 449 D KQ +DQ +T + +++K Sbjct: 397 A-DPKQALDQAAETAKGQIKAK 417 >ref|NP_404477.1| putative sugar-binding protein [Yersinia pestis] ref|NP_670540.1| putative periplasmic binding protein [Yersinia pestis KIM] ref|NP_994828.1| putative sugar-binding protein [Yersinia pestis biovar Mediaevails str. 91001] pir||AD0105 probable sugar-binding protein YPO0856 [imported] - Yersinia pestis (strain CO92) emb|CAC89703.1| putative sugar-binding protein [Yersinia pestis CO92] gb|AAM86791.1| putative periplasmic binding protein [Yersinia pestis KIM] gb|AAS63705.1| putative sugar-binding protein [Yersinia pestis biovar Mediaevails str. 91001] Length = 420 Score = 197 bits (500), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 135/383 (35%), Positives = 201/383 (52%), Gaps = 18/383 (4%) Query: 77 KLMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPH 136 +L VWE ++ +K ADFEK++ V VNV E+ Q +L DGPA + DVL +P+ Sbjct: 39 QLTVWEDIKKSAGIKEAIADFEKQHQVKVNVLEMPYAQQIEKLRLDGPAGIGPDVLVIPN 98 Query: 137 DQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVP 196 DQ+ AV GLL P V + + + ++ A + D LYG PK+VET + YNKD++P Sbjct: 99 DQLGGAVVQGLLTPLSV-DPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLP 157 Query: 197 EAPKTWNDIIAFANTYNKPKENKFAIMWEFVG-YYTYPFIGSFGGYIFG-DSNSNPK--D 252 T ++ AF+ + ENK+ ++ +F YY++ I GGYIFG D+N + K D Sbjct: 158 TPLATLDEYAAFSK--KQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKAND 215 Query: 253 IGLNNEGTIKGFTFLQSL--KKILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWSTGSFK 309 IGLN G ++ T+L++ + P+ GD + LF E K A I+GPW+ ++ Sbjct: 216 IGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYE 275 Query: 310 DH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYKM 368 +NFGV PLP PNG++M SF GVK Y V++++ A+ F F + Y++ Sbjct: 276 AAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYVKTRYQV 335 Query: 369 TGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAF---VSLWN 425 T IPA + IKND Q + MP I E+ VW P +A V+ Sbjct: 336 TKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQ 395 Query: 426 DPGLDVKQTMDQTVKTIQSDLES 448 +P K +D VK I +E+ Sbjct: 396 EP----KVALDNAVKQINMQIEA 414 >ref|NP_980358.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus cereus ATCC 10987] gb|AAS42966.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus cereus ATCC 10987] Length = 419 Score = 193 bits (491), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 126/377 (33%), Positives = 205/377 (54%), Gaps = 9/377 (2%) Query: 78 LMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPHD 137 L+VWE ++ ++ FE+KY V V V E+ DQ +L DGPA DV+TLPHD Sbjct: 40 LLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKKLRLDGPAGTGPDVVTLPHD 99 Query: 138 QISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVPE 197 QI AV GLL ++ K+ +++++A +Y+G LYG PK++ET YNK L+P+ Sbjct: 100 QIGNAVTEGLLSEVKA-DDSIKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMPK 158 Query: 198 APKTWNDIIAFANTYNKPKENKFAIMWEFVGYYTYPFIGSFGGYIFGDSNSNP--KDIGL 255 AP+T +++ F+ + K + F + + Y+ F+ GGY+FG+ + P D+GL Sbjct: 159 APETMDELFNFSKEFTKDGKYGFLALGDNF-YFANAFMAGMGGYVFGEKDGKPNASDVGL 217 Query: 256 NNEGTIKGFTFLQSL--KKILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWSTGSF-KDH 311 NN G ++G +LQ +K+ P G+ LF EGK A ++GPW+ + K+ Sbjct: 218 NNSGAVQGAEYLQKWYKEKLFPKGIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEKNG 277 Query: 312 VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYKMTGA 371 +++GV P+PK PNG+ M +F GVK ++V S++ A F + ++++N ++ T Sbjct: 278 IDYGVAPMPKLPNGQPMKTFVGVKGWHVTSFSKQNDLATKFTEWVTNEENAKIRFEKTKE 337 Query: 372 IPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAFVSLWNDPGLDV 431 IP E IK++ Q ++ +PMP I E+ VW+P A + D Sbjct: 338 IPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKEAP- 396 Query: 432 KQTMDQTVKTIQSDLES 448 K +D VK I+ ++E+ Sbjct: 397 KAALDNAVKQIKGNIEA 413 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.131 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 710,687,734 Number of extensions: 31604169 Number of successful extensions: 71899 Number of sequences better than 1.0e-05: 194 Number of HSP's gapped: 71495 Number of HSP's successfully gapped: 194 Length of query: 449 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 320 Effective length of database: 406,989,807 Effective search space: 130236738240 Effective search space used: 130236738240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)