BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0021.p (1122 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_810681.1| beta-galactosidase [Bacteroides thetaiotaomicro... 225 6e-57 ref|NP_630542.1| putative beta-galactosidase [Streptomyces coeli... 141 9e-32 sp|O52847|BGAL_BACME BETA-GALACTOSIDASE (LACTASE) >gi|7474365|pi... 126 3e-27 dbj|BAA34817.1| beta-galactosidase [Saccharopolyspora rectivirgula] 125 6e-27 sp|Q56307|BGAL_THEMA Beta-galactosidase (Lactase) 125 7e-27 >ref|NP_810681.1| beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76875.1| beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482] Length = 1085 Score = 225 bits (573), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 179/671 (26%), Positives = 298/671 (44%), Gaps = 80/671 (11%) Query: 1 LNGEWDFMPL-------YGQPLNRTLP---ERLVYEARKVQVPSSWRHGYPQPSGKKFGE 50 +NG WDF P+ G+ + LP + ++++PS W Sbjct: 63 INGYWDFQPVRLPKEYVQGKGIAPELPLPENDSEWSKTRIKIPSPWNI------------ 110 Query: 51 VAEHGFAPLDVYG--------YPREWDAAEAGVLHRSFQVPETMLGQRIVLRFDGIMQKA 102 + FA D+ G YP+EW+ + + ++ +P GQ+I L F+ + + Sbjct: 111 ---NAFAYRDLEGPDHRNYPSYPKEWEQVKMAWMKKNVTIPADWSGQQIKLHFEAVAGYS 167 Query: 103 AVYLDRERIAVWEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPS----GDRKITG 158 VY+++E++ D +LP DI+ +V GE ++ ++ G Q++ + G R + G Sbjct: 168 EVYVNKEKVGENFDLFLPFSFDITDKVTAGE--NIEILVGVRSQSLFENNATIGRRIVPG 225 Query: 159 LAGSWFGRMARGIWQDVFLESYPLVTLEDITIRTLFRQGLLEVDALIGTESGSAYAEPLR 218 GS +G GIWQDV+L + P + LED+ ++ L + LE++ + + L+ Sbjct: 226 --GSMWGYQINGIWQDVYLLALPKIHLEDVYVKPLVSKNTLEIEVTLQNRTEKKTDVQLQ 283 Query: 219 ----VFLKVNDRKPGALPVLEAEAGAEAVPLQAGEGRQLCENADNREWQKARFVLSWPDA 274 ++ + PV G EA+ + +L A + + + Sbjct: 284 GNICEWVNCAGTDVNSAPVPNWTLGNEALKVAP---TKLSLPAKASQKVTLQVPVDKDIL 340 Query: 275 RLWSPETPNLYELELELRAGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWHFQ 334 + W+PE PNLY L L + +T+D + FG+RE+ +G LNG P L GDSWHF Sbjct: 341 KYWTPEHPNLYALLLSVNNQKQTVDTKYERFGWREWTLQGTTQYLNGEPYALHGDSWHFM 400 Query: 335 GAAQQTEGYIRNWYRMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYGSSKSM 394 G Q T Y W+ + N++R HA+ YP YL +ADE G+ +++ETA + S Sbjct: 401 GIPQMTRRYAWAWFTAIKGMNANAVRPHAQVYPRFYLDMADEMGICVLNETANWASDGGP 460 Query: 395 DANHPDFIANCRAHVQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMK----- 449 + F + H++R V RD+NH SV WS+ NE + V + + + K Sbjct: 461 KLDSDLFWEASKEHLKRFVLRDRNHASVFGWSISNENKPVILHVYNRPELMPVQKKAWEE 520 Query: 450 ---LMRDLDSTRP-IMVEGDNR---LLPKELTEVESRHYNIDGTISQW-DRTVPLTFGEH 501 ++ D TRP I +G++ +LP V HY ++ +W + P GEH Sbjct: 521 WRDIVHQYDPTRPWISADGEDDGDGILP-----VTVGHYGDINSMKRWIEIGKPWGIGEH 575 Query: 502 GGWWYICPQNSSMYVGLGAYRDTDASAAGLAQKERLFVEYARRQGVSGISTFNFVHYFMR 561 +Y P+ + Y G AY + GLA + + R+ G S + FN Y ++ Sbjct: 576 SMAYYGTPEQVAKYNGERAYESQEGRMEGLANECYNLIVNQRQMGASYSTVFNMAWYALK 635 Query: 562 AMP--DRDIALPPA---------DLTE--PGPKPVLIPAYSLSLNNGLLPEEYPAYRVNP 608 +P +D + P D E PG +P + Y + N G P P Y+ P Sbjct: 636 PLPLGKKDKSKAPTVEADGVFFGDYKEGVPGVQPERVGPYCTTFNPGYDP-TLPLYQEWP 694 Query: 609 SFDIMAEAFKP 619 + + A P Sbjct: 695 MYSALRAANAP 705 >ref|NP_630542.1| putative beta-galactosidase [Streptomyces coelicolor A3(2)] pir||T35944 probable beta-galactosidase - Streptomyces coelicolor emb|CAA22766.1| putative beta-galactosidase [Streptomyces coelicolor A3(2)] Length = 1307 Score = 141 bits (356), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 118/405 (29%), Positives = 179/405 (44%), Gaps = 43/405 (10%) Query: 74 GVLHRSFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAVWEDGYLPLRLDISSRVKPGE 133 G R+F VP G+R L F+G+ V+++ E ED Y P D++ +KPG Sbjct: 181 GQYRRTFTVPRDWRGRRTFLHFEGVKSAHYVWINGELAGYDEDSYTPSEYDVTDLLKPGT 240 Query: 134 EQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARGIWQDVFLESYPLVTLEDITIRTL 193 Q V D + D+ + L+G I++ V L S P V L D + T Sbjct: 241 NQIAVEVYRYSDGDWME--DQDMIRLSG---------IFRSVHLYSTPAVHLRDFKLDTP 289 Query: 194 FRQGLLEVDALIGTESGSAYAEP----LRVFLKVNDRKPGALPVLEAEAGAEAVPLQAGE 249 + + T S AY E V ++ D + A+ + + +AGE Sbjct: 290 LDDTYTGAELAV-TASVRAYGEGGTGRYTVETQLYDARGHAVWSRPLQQPVDVGGARAGE 348 Query: 250 GRQLCENADNREWQKARFVLSWPDARLWSPETPNLYELELELR-AGGETLDRRTGTFGFR 308 + R + P RLWS E P+LY L LR G+ + + GFR Sbjct: 349 DVTV------------RAAKAVPKPRLWSAEHPDLYTAVLRLRDPAGKVTETLSHRVGFR 396 Query: 309 EFWCEGPQFMLNGTPVNLRGDSWH----FQGAAQQTEGYIRNWYRMCRQAGVNSIRLHAE 364 EF + +NG PV++RG + H +G A E + + ++ ++ +NS+R Sbjct: 397 EFALKDGLMRINGEPVSIRGTNRHEMHPARGTALTREDMVED-IKIIKRMNMNSVRTSHY 455 Query: 365 PYPSDYLRIADEEGMLIVDETAI--YGSSKSMDANHPDFIANCRAHVQRLVQRDKNHPSV 422 P +L +ADE G+ +VDET + +G +HPD+ A C A Q +V RDKNH SV Sbjct: 456 PNNPYWLELADEYGLYLVDETNLETHGIRGEYPGDHPDWTAACVARAQNMVHRDKNHASV 515 Query: 423 ILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRPIMVEGDNR 467 ++WS+ NE G + +R D TR I EGD+R Sbjct: 516 VIWSLGNEA-------GGGSTFSAMHDWIRSYDDTRVIQYEGDDR 553 >sp|O52847|BGAL_BACME BETA-GALACTOSIDASE (LACTASE) pir||T30574 beta-galactosidase - Bacillus megaterium emb|CAA04267.1| beta-galactosidase [Bacillus megaterium] Length = 1034 Score = 126 bits (316), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 136/510 (26%), Positives = 217/510 (42%), Gaps = 74/510 (14%) Query: 1 LNGEWDFM---PLYGQPLNRTLPERLVYEARKVQVPSSWR---HGYPQPSGKKF----GE 50 LNG W F G+ N PE + + VPS W+ + YPQ + + E Sbjct: 61 LNGSWYFHFAENADGRVKNFFAPEFSYEKWDSISVPSHWQLQGYDYPQYTNVTYPWVENE 120 Query: 51 VAEHGFAPLDVYGYPREWDAAEAGVLHRSFQVPETMLGQRIVLRFDGIMQKAAVYLDRER 110 E FAP G R+F Q + + F G+ V+++ E Sbjct: 121 ELEPPFAPTKY---------NPVGQYVRTFTPKSEWKDQPVYISFQGVESAFYVWINGEF 171 Query: 111 IAVWEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARG 170 + ED + P DI+S ++ GE V D + L D F RM+ G Sbjct: 172 VGYSEDSFTPAEFDITSYLQEGENTIAVEVYRWSDASWLEDQD----------FWRMS-G 220 Query: 171 IWQDVFLESYPLVTLEDITIRTL----FRQGLLEVDALIGTESGSAYAEPLRVFLKVNDR 226 I++DV+L S P V + D ++R+ + G L V A I L F +D Sbjct: 221 IFRDVYLYSTPQVHIYDFSVRSSLDNNYEDGELSVSADI-----------LNYF--EHDT 267 Query: 227 KPGALPVLEAEAGAEAVPLQAGEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYE 286 + V+ +A A+ V LQA L +D R + S WS E+PNLY Sbjct: 268 QDLTFEVMLYDANAQEV-LQAPLQTNLSV-SDQRTVSLRTHIKS---PAKWSAESPNLYT 322 Query: 287 LELELR-AGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWH----FQGAAQQTE 341 L L L+ A G ++ + GFR F + +NG + LRG + H +G A T Sbjct: 323 LVLSLKNAAGSIIETESCKVGFRTFEIKNGLMTINGKRIVLRGVNRHEFDSVKGRAGITR 382 Query: 342 GYIRNWYRMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIY----------GSS 391 + + + +Q +N++R P S + + +E G+ ++DET + G Sbjct: 383 EDMIHDILLMKQHNINAVRTSHYPNDSVWYELCNEYGLYVIDETNLETHGTWTYLQEGEQ 442 Query: 392 KSMDANHPDFIANCRAHVQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKLM 451 K++ + P++ N + + +RDKNHPS+I+WS+ NE G + + QH + Sbjct: 443 KAVPGSKPEWKENVLDRCRSMYERDKNHPSIIIWSLGNESF---GGENF-QH---MYTFF 495 Query: 452 RDLDSTRPIMVEGDNRLLPKELTEVESRHY 481 ++ DSTR + EG + +++ES Y Sbjct: 496 KEKDSTRLVHYEGIFHHRDYDASDIESTMY 525 >dbj|BAA34817.1| beta-galactosidase [Saccharopolyspora rectivirgula] Length = 1251 Score = 125 bits (314), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 133/496 (26%), Positives = 203/496 (40%), Gaps = 87/496 (17%) Query: 1 LNGEWDFMPLYGQPLNRTLP---ERLVYEARKVQVPSSWRH-GYPQPSGKKFG-EVAEHG 55 LNG W F Y R LP +R E V VP W+ GY +P + E+A Sbjct: 91 LNGRWQFQ--YADHY-RDLPAGWQRGSGEWDAVDVPGVWQQQGYDRPIYRNVSSEIAP-- 145 Query: 56 FAPLDVYGYPREWDAAEAGVLHRSFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAVWE 115 + P +V D G + F++PE G+R +LRF+G+ V+++ + + Sbjct: 146 YRPPEVPD-----DINPTGAYRKQFELPEHWAGRRQLLRFEGVTSGYFVWVNGHYVGYDQ 200 Query: 116 DGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMA----RGI 171 GY P D++ ++PG T+ R +G S+ M GI Sbjct: 201 GGYTPAEFDVTPHLRPGT------------NTIAVQVHRWSSG---SYLENMDFWHFSGI 245 Query: 172 WQDVFLESYPLVTLEDITIRTLFRQGLLEVDALIGTE-----SGSAYAEPLRVFLKVNDR 226 +++V L S P LED+T+RT + +G E G+ LR L D Sbjct: 246 FREVHLYSVPRTHLEDVTVRTELDDTYTDATLRLGAELRHLPGGTTGRHELRATLYDPD- 304 Query: 227 KPGALPVLEAEAGAEAVPLQAGEGRQLCENAD----NREWQKARFVLSWPDARLWSPETP 282 G ++ E ++ +RE A RLWS ETP Sbjct: 305 -----------------------GNRVAEVSEPVVVDRETASAELAQHVAAPRLWSDETP 341 Query: 283 NLYELELELRAGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWHFQ----GAAQ 338 +LY LEL G+ + GFR Q +LNG PV LRG + H G A Sbjct: 342 HLYTAVLELLHNGQVVHTTQQPVGFRSVEVRDEQLLLNGKPVELRGVNRHEHDPRTGRAV 401 Query: 339 QTEGYIRNWYRMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYG------SSK 392 + ++ + RQ +N++R P + R+AD G+L+ DE + S+ Sbjct: 402 SRQRQQQD-VELLRQHNINAVRTSHYPNDPYWYRLADHHGILLADEVDVETHYREDCSNP 460 Query: 393 SMD--ANHPDFIANCRAHVQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKL 450 + D A+ P++ A L++RDKNHPSVI+W NE G + + Sbjct: 461 ADDCLADRPEWQAAFADRFHALLERDKNHPSVIIWDTGNEA-------GLGAAHYAMAEH 513 Query: 451 MRDLDSTRPIMVEGDN 466 R D TRP+ + ++ Sbjct: 514 ARATDPTRPLYHQSNS 529 >sp|Q56307|BGAL_THEMA Beta-galactosidase (Lactase) Length = 1084 Score = 125 bits (314), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 130/486 (26%), Positives = 212/486 (43%), Gaps = 75/486 (15%) Query: 1 LNGEWDFMPLYGQPLNRTLPERLVYEA------RKVQVPSSWR-HGYPQPSGKKFGEVAE 53 LNG W F+ P +PE E +++VPS+W GY GK Sbjct: 42 LNGNWRFL-FAKNPFE--VPEDFFSEKFDDSNWDEIEVPSNWEMKGY----GKPIYTNVV 94 Query: 54 HGFAPLDVYGYPREWDAAEAGVLHRSFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAV 113 + F P + P+ D GV R ++PE + I L F+G+ ++++ ++I Sbjct: 95 YPFEPNPPF-VPK--DDNPTGVYRRWIEIPEDWFKKEIFLHFEGVRSFFYLWVNGKKIGF 151 Query: 114 WEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARGIWQ 173 +D P ++ ++PG+ V D + L D WF GI++ Sbjct: 152 SKDSCTPAEFRLTDVLRPGKNLITVEVLKWSDGSYLEDQDM-------WWFA----GIYR 200 Query: 174 DVFLESYPLVTLEDITIRTLFRQGLLEVDALIGTESGSAYAEPLRVFLKVNDRKPGALPV 233 DV+L + P + D+ +RT D +G ++FL V R G Sbjct: 201 DVYLYALPKFHIRDVFVRT---------DLDENYRNG-------KIFLDVEMRNLGE--- 241 Query: 234 LEAEAGAEAVPLQA-GEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYELELELR 292 E E E + G+ + L + E + F D + WS ETP+LY L+L+L Sbjct: 242 -EEEKDLEVTLITPDGDEKTLVKETVKPEDRVLSFAFDVKDPKKWSAETPHLYVLKLKL- 299 Query: 293 AGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWHF----QGAAQQTEGYIRNWY 348 GE D + FGFR+ + + NG P+ ++G + H +G A E I++ Sbjct: 300 --GE--DEKKVNFGFRKIEIKDGTLLFNGKPLYIKGVNRHEFDPDRGHAVTVERMIQD-I 354 Query: 349 RMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYGSSKSMDANHPDFIANC--- 405 ++ +Q +N++R P + + + D G+ ++DE I S +D + +AN Sbjct: 355 KLMKQHNINTVRTSHYPNQTKWYDLCDYFGLYVIDEANI--ESHGIDWDPEVTLANRWEW 412 Query: 406 -RAH---VQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRPIM 461 +AH ++R+V+RDKNHPS+I WS+ NE DG L ++ D+TR I Sbjct: 413 EKAHFDRIKRMVERDKNHPSIIFWSLGNE-----AGDGVNFEKAALW--IKKRDNTRLIH 465 Query: 462 VEGDNR 467 EG R Sbjct: 466 YEGTTR 471 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,717,646,277 Number of extensions: 75761322 Number of successful extensions: 146076 Number of sequences better than 1.0e-05: 335 Number of HSP's gapped: 145388 Number of HSP's successfully gapped: 341 Length of query: 1122 Length of database: 757,237,578 Length adjustment: 137 Effective length of query: 985 Effective length of database: 385,269,015 Effective search space: 379489979775 Effective search space used: 379489979775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)