BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0008.p (326 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAE02636.1| Ycxc protein [Bacillus amyloliquefaciens] 228 2e-58 ref|ZP_00099177.1| COG0697: Permeases of the drug/metabolite tra... 225 9e-58 ref|NP_388237.1| similar to hypothetical proteins from B. subtil... 223 5e-57 gb|AAD04761.1| unknown protein [Bacillus licheniformis] 206 4e-52 ref|NP_348165.1| Predicted permease, YCXC B.subtilis ortholog [C... 204 2e-51 >emb|CAE02636.1| Ycxc protein [Bacillus amyloliquefaciens] Length = 378 Score = 228 bits (580), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 130/286 (45%), Positives = 184/286 (64%) Query: 28 IIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKPIGRALLLAAM 87 IIGFSF+F K+AL A P D L +RF A+F+ +PV FGW+K+S K + L LA + Sbjct: 78 IIGFSFIFVKIALRSANPFDILAHRFTAAFICAMLPVLFGWIKLSVSWKNVLAILPLALL 137 Query: 88 YPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKLCIFLSVFGVV 147 YP FF+ Q FGL +++S+E GI+ + P+ TM+ +S FLKE T QK LSV GVV Sbjct: 138 YPALFFSFQAFGLLYSSSSEAGIIQAAIPIFTMLFSSYFLKERPTWAQKGFTCLSVAGVV 197 Query: 148 FIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYLLMGIGFATFL 207 FIF MKG S + ++ G L+ L+ L+ A Y+ AR ++ FS EL++ + IGFA+F Sbjct: 198 FIFTMKGVSAESASFKGAFLILLSALSSALYNTAARRMTLRFSLGELTWCMSAIGFASFN 257 Query: 208 ILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSKIEASLMSVFS 267 ++L H A GT+G + P + FI + +YLG+++SL+T+L S Y LSKIEA MS F+ Sbjct: 258 CIALFRHGAEGTIGQYFLPFRDPGFIAAILYLGILSSLITSLMSNYALSKIEAFKMSAFN 317 Query: 268 NLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNRLGRKKTE 313 NLS IV++ AG L L E + YH G+ I+ GV+G+N GR+K Sbjct: 318 NLSVIVTMLAGVLVLHESLRWYHIAGAVCIVIGVLGSNASGRRKKS 363 >ref|ZP_00099177.1| COG0697: Permeases of the drug/metabolite transporter (DMT) superfamily [Desulfitobacterium hafniense] Length = 313 Score = 225 bits (574), Expect = 9e-58, Method: Compositional matrix adjust. Identities = 123/308 (39%), Positives = 191/308 (62%) Query: 16 KLAYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRG 75 ++AY+ A++ A IIG SF+F K+ L A PLDTL +RF +F+ ++ + F KV+ Sbjct: 5 RMAYLAAIIYAFIIGLSFMFVKLTLTVATPLDTLAHRFTVAFLAATLFIFFTKHKVNIGW 64 Query: 76 KPIGRALLLAAMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQ 135 + + LA +YP+ FF Q+FGL TS+E GI+ S P+ T+++A LKE Q Sbjct: 65 HDVAQIAPLATLYPILFFGFQIFGLARTTSSEAGIIQSTVPIFTLLLAVFILKEKVGRGQ 124 Query: 136 KLCIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELS 195 + +FLSVFGV+++ M G+ Q +N+ G VL+ L+ A Y+VLAR L++ +S L+ Sbjct: 125 LISVFLSVFGVIYLLAMSGAESQTANIVGSVLIILSAFTNALYNVLARRLTQRYSLLTLT 184 Query: 196 YLLMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYIL 255 Y++ GF F L++ +GT+G F PL F+++ +YLG+++SLVT+ + ++L Sbjct: 185 YIMTLFGFIAFNSLAIGSRVLAGTVGEFFQPLLHWQFVVAVLYLGILSSLVTSFLANFVL 244 Query: 256 SKIEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNRLGRKKTEAR 315 SKIEA+ MSVFSN++T+++I AG LFL E YH IG +II GV+GTN +G + Sbjct: 245 SKIEAAKMSVFSNVATLITILAGILFLQEAFHFYHVIGGMMIITGVVGTNLMGARSKAKE 304 Query: 316 PGGDRTVP 323 + V Sbjct: 305 KAKTKDVE 312 >ref|NP_388237.1| similar to hypothetical proteins from B. subtilis [Bacillus subtilis] sp|Q08794|YCXC_BACSU Hypothetical transport protein ycxC pir||I40491 conserved hypothetical protein ycxC - Bacillus subtilis emb|CAA49823.1| unnamed protein product [Bacillus subtilis] dbj|BAA08989.1| homologue of hypothetical protein of Buchnera aphidicola [Bacillus subtilis] emb|CAB12149.1| ycxC [Bacillus subtilis subsp. subtilis str. 168] Length = 312 Score = 223 bits (568), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 134/300 (44%), Positives = 195/300 (65%), Gaps = 1/300 (0%) Query: 18 AYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKP 77 AYI A+L + IIG SFLF K+AL A P D L +RF +F +VP+ FGWVK+S K Sbjct: 9 AYIAAILYSFIIGLSFLFVKIALQTAEPFDILAHRFTIAFAAATVPILFGWVKLSILVKD 68 Query: 78 IGRALLLAAMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKL 137 + L LA +YP FF+ Q FGL +++S+E GI+ + P+ TM+ A+ LKE T QK Sbjct: 69 VIDILPLALLYPALFFSFQAFGLVYSSSSEAGIIQAAIPIFTMVFAAYVLKERPTWTQKG 128 Query: 138 CIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYL 197 LSV GV+FIFVMKG ++ +++ G +L+ L+ L+ A Y+ AR +++ F EL+Y+ Sbjct: 129 FTVLSVAGVMFIFVMKGVDVESASLKGSLLILLSALSSAMYNTAARKMTQRFKLTELTYI 188 Query: 198 LMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSK 257 + IGF F ++L H A+GT+G + P + F+L+ VYLGV++SLVT+ S Y LS+ Sbjct: 189 MSAIGFVVFNAIALVRHGAAGTVGTYFLPFREPGFVLAIVYLGVLSSLVTSFLSNYTLSR 248 Query: 258 IEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNRLGRKKTEARPG 317 IEA MS F+++STIV++ AG + L E + YH G+ I+ GV+G+N KKT+ RPG Sbjct: 249 IEAFKMSAFNHVSTIVTMIAGFVILNESLAWYHLAGAVCIMIGVVGSNINLEKKTK-RPG 307 >gb|AAD04761.1| unknown protein [Bacillus licheniformis] Length = 344 Score = 206 bits (525), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 121/273 (44%), Positives = 172/273 (63%) Query: 31 FSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKPIGRALLLAAMYPL 90 FSF F K+AL A P+D L +RF +F+ P+ GWVK+ K + L LA YP+ Sbjct: 27 FSFFFVKLALTAANPVDILAHRFTVAFLAALFPLLLGWVKIGISMKDALKVLPLALFYPV 86 Query: 91 GFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKLCIFLSVFGVVFIF 150 FF QVFGL + +S+E GI+ + P+ TMI A+ FL E T Q LS GV IF Sbjct: 87 MFFAFQVFGLVYTSSSEAGIIQAAIPIFTMIFAAYFLNERFTTRQFFFTVLSAAGVACIF 146 Query: 151 VMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYLLMGIGFATFLILS 210 +MK + S++ G L+ L+ L+ AGYSVLAR ++R F+ EL+Y+ G GF F ++ Sbjct: 147 MMKSAGTGSSDLRGAALILLSALSSAGYSVLARKMTRTFNSLELTYVTTGAGFLFFNGIA 206 Query: 211 LTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSKIEASLMSVFSNLS 270 H A GTL GF +P FILS +YLGV++S T+ S Y LSKIEA+ +S+F++L+ Sbjct: 207 GISHMADGTLSGFFAPFTDPVFILSVLYLGVLSSFATSFLSNYALSKIEAAKISMFNHLA 266 Query: 271 TIVSIAAGALFLGEEITVYHWIGSFLIIAGVIG 303 T+V+I AG ++LGE + +H+ G+ +IIAG+IG Sbjct: 267 TLVTILAGIIWLGESLEWFHFAGTGMIIAGLIG 299 >ref|NP_348165.1| Predicted permease, YCXC B.subtilis ortholog [Clostridium acetobutylicum] pir||F97089 probable permease, YCXC B. subtilis ortholog [imported] - Clostridium acetobutylicum gb|AAK79505.1| Predicted permease, YCXC B.subtilis ortholog [Clostridium acetobutylicum] Length = 299 Score = 204 bits (519), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 121/297 (40%), Positives = 180/297 (60%) Query: 10 MQQQGLKLAYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWV 69 M + L YI A +++ I G SFLF+K+AL AGPL + RF +F++M++ V F + Sbjct: 1 MNGKQKILPYIAATVSSTIFGLSFLFSKMALKVAGPLSLVALRFLLAFIIMTILVLFRVI 60 Query: 70 KVSYRGKPIGRALLLAAMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKE 129 KV YR KPIG+ +LL P+ +F + +G+++ +S+ G++ + P+V I+ S FLKE Sbjct: 61 KVDYRNKPIGQLVLLGLAEPVIYFIFETYGVKNISSSMAGLMLALIPIVVTIMGSYFLKE 120 Query: 130 TTTLLQKLCIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHF 189 +L + I LSV GV I VM S ++ GI+ L YS+++R S HF Sbjct: 121 VPSLKRTSFIVLSVSGVALIGVMGSSGGSGNSPIGILFLLGAVTCAGAYSIISRKTSVHF 180 Query: 190 SPAELSYLLMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTL 249 P E++Y +M F I++ A G + + SPLKS TF++S +YLG+++S+V Sbjct: 181 KPVEITYFMMFFAAFCFNIMAAIQLLAEGKINEYFSPLKSSTFVISILYLGILSSIVAYF 240 Query: 250 TSTYILSKIEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNR 306 + + LSKI+AS SVFSNLSTIVSI AG + L E +YH +GS LI+ GV GTN+ Sbjct: 241 LTNFTLSKIQASRSSVFSNLSTIVSIIAGVIILKESFHLYHLVGSVLILTGVWGTNK 297 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.141 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 416,779,207 Number of extensions: 15828531 Number of successful extensions: 70998 Number of sequences better than 1.0e-05: 386 Number of HSP's gapped: 71279 Number of HSP's successfully gapped: 386 Length of query: 326 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 200 Effective length of database: 415,135,104 Effective search space: 83027020800 Effective search space used: 83027020800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 126 (53.1 bits)