BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0164.0014.p (351 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244642.1| peptide chain release factor 1 in translation [... 472 e-132 ref|NP_847729.1| peptide chain release factor 1 [Bacillus anthra... 467 e-130 ref|NP_834991.1| Bacterial Peptide Chain Release Factor 1 (RF-1)... 466 e-130 ref|NP_391582.1| peptide chain release factor 1 [Bacillus subtil... 447 e-124 ref|NP_466066.1| highly similar to peptide chain release factor ... 445 e-124 >ref|NP_244642.1| peptide chain release factor 1 in translation [Bacillus halodurans] sp|Q9K6F4|RF1_BACHD Peptide chain release factor 1 (RF-1) pir||G84121 peptide chain release factor 1 in translation prfA [imported] - Bacillus halodurans (strain C-125) dbj|BAB07494.1| peptide chain release factor 1 in translation [Bacillus halodurans] Length = 356 Score = 472 bits (1215), Expect = e-132, Method: Compositional matrix adjust. Identities = 223/351 (63%), Positives = 286/351 (81%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQSL +RYE+L+ELL DP+V SDS KLR+YSKEQ+D++ + Y EYK VM++ + AK M Sbjct: 5 LQSLDERYERLNELLSDPEVISDSNKLREYSKEQADMEETVQVYREYKTVMDQYQDAKEM 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 +KLDDEM MVK EI++L++R+ LE K++ VIVE+RGAAGGDEA Sbjct: 65 LEDKLDDEMYAMVKEEIDELATRKEDLEAKLKVLLLPKDPNDDKNVIVEVRGAAGGDEAQ 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA+DLY+MY RYA+AQGW++E+++ ++ +LGG+KE+IF +NG+GAYSK+K+E+GAHRVQ Sbjct: 125 LFAADLYKMYARYAEAQGWKIEVIEASSTELGGYKEIIFSVNGKGAYSKLKYENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE E++I +KDIRVDTF SSG GGQSVNTT SAVR+ Sbjct: 185 RVPQTESGGRIHTSTATVAVLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTGIV +CQD KSQ NKEKA++VLRARI D +QE +A+Y RKS VGTGDRSER Sbjct: 245 THLPTGIVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQSRVTDHRIGLTL +L+Q++ G + EII +L + EQAELM++ E Sbjct: 305 IRTYNFPQSRVTDHRIGLTLQKLEQILQGKLDEIIESLIVHEQAELMKQQE 355 >ref|NP_847729.1| peptide chain release factor 1 [Bacillus anthracis str. Ames] ref|NP_981748.1| peptide chain release factor 1 [Bacillus cereus ATCC 10987] gb|AAP29215.1| peptide chain release factor 1 [Bacillus anthracis str. Ames] gb|AAS44356.1| peptide chain release factor 1 [Bacillus cereus ATCC 10987] Length = 355 Score = 467 bits (1202), Expect = e-130, Method: Compositional matrix adjust. Identities = 219/351 (62%), Positives = 281/351 (80%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ +RYEKL+ELL DP + SDS KLR+YSKEQSD+Q E Y EYK+V E+L+ AK M Sbjct: 5 LQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAM 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 +KLD EM+EMVK E+ +L S++ L E+++ VIVE+RGAAGGDEAA Sbjct: 65 LEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAA 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DLYRMY+RYA+ QGW+ E+++ + +LGG+KE+IFMING+GA++K+KFE+GAHRVQ Sbjct: 125 LFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE EI+I +KD+RVDTF SSG GGQSVNTT SAVR+ Sbjct: 185 RVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTG+V +CQD KSQ NKEKA++VLRAR+ D RQE +A+Y RK VGTGDRSER Sbjct: 245 THLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQ+RVTDHRIGLT+ +LDQ++ G + + I+AL + +QA+ ME E Sbjct: 305 IRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE 355 >ref|NP_834991.1| Bacterial Peptide Chain Release Factor 1 (RF-1) [Bacillus cereus ATCC 14579] gb|AAP12192.1| Bacterial Peptide Chain Release Factor 1 (RF-1) [Bacillus cereus ATCC 14579] Length = 358 Score = 466 bits (1200), Expect = e-130, Method: Compositional matrix adjust. Identities = 219/351 (62%), Positives = 280/351 (79%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ +RYEKL+ELL DP + SDS KLR+YSKEQSD+Q E Y EYK+V E+L+ AK M Sbjct: 8 LQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAM 67 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 +KLD EM+EMVK E+ +L S+ L E+++ VIVE+RGAAGGDEAA Sbjct: 68 LEDKLDAEMREMVKEEVSELESQDKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAA 127 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DLYRMY+RYA+ QGW+ E+++ + +LGG+KE+IFMING+GA++K+KFE+GAHRVQ Sbjct: 128 LFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGAHRVQ 187 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+V+PEAEE EI+I +KD+RVDTF SSG GGQSVNTT SAVR+ Sbjct: 188 RVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMSAVRL 247 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTG+V +CQD KSQ NKEKA++VLRAR+ D RQE +A+Y RK VGTGDRSER Sbjct: 248 THLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSER 307 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQ+RVTDHRIGLT+ +LDQ++ G + + I+AL + +QA+ ME E Sbjct: 308 IRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQKMEAAE 358 >ref|NP_391582.1| peptide chain release factor 1 [Bacillus subtilis] sp|P45872|RF1_BACSU Peptide chain release factor 1 (RF-1) pir||S55437 translation releasing factor RF-1 - Bacillus subtilis emb|CAA89884.1| peptide chain release factor 1 [Bacillus subtilis] emb|CAB15718.1| peptide chain release factor 1 [Bacillus subtilis subsp. subtilis str. 168] Length = 356 Score = 447 bits (1151), Expect = e-124, Method: Compositional matrix adjust. Identities = 211/351 (60%), Positives = 273/351 (77%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 L+S+ +RYEKL+ELL DP+V +D KKLR+YSKEQSD+Q + Y +Y++ E+L AK M Sbjct: 5 LKSIEERYEKLNELLSDPEVVNDPKKLREYSKEQSDIQETVDVYRQYRDASEQLADAKAM 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 EKLD EM++MVK EI +L L E+++ VI+EIRGAAGG+EAA Sbjct: 65 LEEKLDAEMRDMVKEEISELQKETETLSERLKVLLIPKDPNDDKNVIMEIRGAAGGEEAA 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA +LYRMY+RYA+ QGW+ E+M+ N GG+KE+IFMI G GAYSK+K+E+GAHRVQ Sbjct: 125 LFAGNLYRMYSRYAELQGWKTEVMEANVTGTGGYKEIIFMITGSGAYSKLKYENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+ +PEAEE E++I +KDIRVDTF SSG GGQSVNTT SAVR+ Sbjct: 185 RVPETESGGRIHTSTATVACLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTG+V +CQD KSQ NKEKA++VLRARI D +QE +A+Y RKS VG+GDRSER Sbjct: 245 THLPTGVVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGSGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELMEKGE 351 IRTYNFPQ+RVTDHRIGLT+ +LDQ++ G + E++ AL + +QA +++ E Sbjct: 305 IRTYNFPQNRVTDHRIGLTIQKLDQILEGKLDEVVEALIVEDQASKLQQSE 355 >ref|NP_466066.1| highly similar to peptide chain release factor 1 [Listeria monocytogenes EGD-e] sp|Q8Y4A8|RF1_LISMO Peptide chain release factor 1 (RF-1) pir||AG1392 peptide chain release factor 1 homolog prf1 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00621.1| prf1 [Listeria monocytogenes] Length = 358 Score = 445 bits (1145), Expect = e-124, Method: Compositional matrix adjust. Identities = 210/348 (60%), Positives = 269/348 (77%) Query: 1 LQSLADRYEKLSELLCDPDVASDSKKLRDYSKEQSDLQPAFEAYAEYKNVMEELEAAKMM 60 LQ++ DRY++L+ELL DPDV SD K+LRD SKEQS + E Y EYKNV E++ K + Sbjct: 5 LQAVEDRYDELNELLSDPDVVSDPKRLRDLSKEQSGITATVETYREYKNVNEQINETKEL 64 Query: 61 QGEKLDDEMKEMVKMEIEDLSSRQTALEEKIRXXXXXXXXXXXXXVIVEIRGAAGGDEAA 120 GEKLDDEM+EM K E +L +T LEE+++ VI+EIRGAAGGDEAA Sbjct: 65 LGEKLDDEMREMAKEEFAELQKEKTDLEERLKLLLVPKDPNDDKNVILEIRGAAGGDEAA 124 Query: 121 LFASDLYRMYTRYADAQGWRVELMDVNTNDLGGFKEVIFMINGRGAYSKMKFESGAHRVQ 180 LFA DL+RMY++YA+++GW+VE+MD N +GG+KE+I M+NG A+S+MK+E+GAHRVQ Sbjct: 125 LFAGDLFRMYSKYAESRGWKVEIMDANPTGIGGYKEIIAMMNGNDAFSRMKYENGAHRVQ 184 Query: 181 RIPTTESGGRIHTSTSTVSVMPEAEEFEIEILDKDIRVDTFCSSGAGGQSVNTTKSAVRV 240 R+P TESGGRIHTST+TV+++PEAEE EIE+ DKDIR DTF S+GAGGQSVNTT SAVR+ Sbjct: 185 RVPETESGGRIHTSTATVAILPEAEEVEIELHDKDIRTDTFASTGAGGQSVNTTMSAVRL 244 Query: 241 THVPTGIVATCQDGKSQNSNKEKALQVLRARISDMKRQEEEAKYAGERKSKVGTGDRSER 300 TH+PTGIV + QD +SQ NK+KA++VLRAR+ D +E +Y RKS VGTGDRSER Sbjct: 245 THIPTGIVVSMQDERSQLKNKDKAMKVLRARVYDKFEREAREEYDANRKSAVGTGDRSER 304 Query: 301 IRTYNFPQSRVTDHRIGLTLHRLDQVMNGDITEIISALSIAEQAELME 348 IRTYN+PQ+RVTDHRIGLT+ +LDQ+M G + EII AL + +Q +E Sbjct: 305 IRTYNYPQNRVTDHRIGLTIQKLDQIMEGKLDEIIDALILEDQTSKLE 352 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.129 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 457,921,108 Number of extensions: 17984837 Number of successful extensions: 51434 Number of sequences better than 1.0e-05: 1399 Number of HSP's gapped: 50207 Number of HSP's successfully gapped: 1400 Length of query: 351 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 225 Effective length of database: 415,135,104 Effective search space: 93405398400 Effective search space used: 93405398400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 126 (53.1 bits)