BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0034.p (251 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00062039.1| COG1131: ABC-type multidrug transport system,... 252 7e-66 ref|NP_782113.1| transporter [Clostridium tetani E88] >gi|282036... 241 1e-62 ref|ZP_00097674.1| COG1131: ABC-type multidrug transport system,... 224 2e-57 emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] 191 1e-47 ref|NP_842835.1| bacitracin ABC transporter, ATP-binding protein... 187 2e-46 >ref|ZP_00062039.1| COG1131: ABC-type multidrug transport system, ATPase component [Clostridium thermocellum ATCC 27405] Length = 241 Score = 252 bits (643), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 114/241 (47%), Positives = 170/241 (70%) Query: 1 MESIAKLTGVSKEFHNGRGISGVNLELNMGDIYGLLGPNGAGKTTLLKMLSGLIAPSFGS 60 ME++ ++ G++K + NGRGI +NLE+ GD++G LGPNGAGKTT +K+++GL+ P+ G Sbjct: 1 METVLEIKGLTKLYKNGRGIFDINLEVYRGDVFGFLGPNGAGKTTAMKIMTGLMHPNSGD 60 Query: 61 ISLFGQDLEQSFASSMRKVGCMIESADFYDYVTARQYLKLNASFYPELPPQRIDEVLEAV 120 + +FG + F +M+KVGC+IE+A+ Y Y+T + LKL A FY ++ +RIDEVLE Sbjct: 61 VKIFGHSVITEFEQAMKKVGCIIETAESYAYLTPYENLKLFARFYDDVDDKRIDEVLELT 120 Query: 121 GLAAFAKEKIRHFSTGMKQKLAMASSILHHPEFVIWDEPTNGLDIEGVVHFRALVKQLSG 180 G+ F KEK+++FS GMKQ+L +A++IL PE VI DEP NGLD+EG++ R L+K L+ Sbjct: 121 GILKFKKEKVKNFSLGMKQRLGLAAAILSRPELVILDEPLNGLDVEGMIEIRKLIKALAE 180 Query: 181 EQGITFLISSHMIHELEQLCNRVGILQQGVLVREGNVHELLADNTSLEQYYIAELQKGKE 240 + TF ISSH+IH++E CNR+GIL G LV +L + SLE Y+++E+ K + Sbjct: 181 NEKTTFFISSHLIHDVEITCNRIGILYDGKLVNVDTTENILKNFASLENYFVSEVGKNES 240 Query: 241 L 241 + Sbjct: 241 V 241 >ref|NP_782113.1| transporter [Clostridium tetani E88] gb|AAO36050.1| transporter [Clostridium tetani E88] Length = 241 Score = 241 bits (614), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 106/237 (44%), Positives = 166/237 (70%) Query: 1 MESIAKLTGVSKEFHNGRGISGVNLELNMGDIYGLLGPNGAGKTTLLKMLSGLIAPSFGS 60 ME + ++T ++K + NGRGI+ +NLE+N GDI+G LGPNGAGKTT +K+++GLI+ G Sbjct: 1 MEKVIEITNLNKVYKNGRGITDINLEINRGDIFGFLGPNGAGKTTTMKIMTGLISADSGD 60 Query: 61 ISLFGQDLEQSFASSMRKVGCMIESADFYDYVTARQYLKLNASFYPELPPQRIDEVLEAV 120 + + G + + F +M+KVGC+IE+A+ Y Y+TA + LK + +Y + +RIDEVLE Sbjct: 61 VKILGHSVIEDFEKAMKKVGCIIETAETYQYLTAYENLKQFSRYYKNVDDKRIDEVLELT 120 Query: 121 GLAAFAKEKIRHFSTGMKQKLAMASSILHHPEFVIWDEPTNGLDIEGVVHFRALVKQLSG 180 G+ F EK FS GMKQ+L +A++IL PE VI DEP NGLD+EG++ R ++K L+ Sbjct: 121 GIIKFKNEKAGKFSLGMKQRLGIAAAILSKPEVVILDEPFNGLDVEGMIDMRNIIKNLAK 180 Query: 181 EQGITFLISSHMIHELEQLCNRVGILQQGVLVREGNVHELLADNTSLEQYYIAELQK 237 E+G TF ISSH+IH++E C ++G+L G ++ +L ++ +LE Y+++E+++ Sbjct: 181 EEGNTFFISSHLIHDVELTCTKIGVLYGGKMLNVDTTKNILNNHATLESYFVSEVEQ 237 >ref|ZP_00097674.1| COG1131: ABC-type multidrug transport system, ATPase component [Desulfitobacterium hafniense] Length = 247 Score = 224 bits (571), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 101/250 (40%), Positives = 170/250 (68%), Gaps = 6/250 (2%) Query: 1 MESIAKLTGVSKEFHNGRGISGVNLELNMGDIYGLLGPNGAGKTTLLKMLSGLIAPSFGS 60 ME + ++ ++K + NGRGI+ ++L++ G+I+G LGPNGAGKTT +K+++GLIAP G Sbjct: 1 MEKVIEIQNLTKLYKNGRGINDLSLDIYEGEIFGFLGPNGAGKTTAMKIMTGLIAPDRGD 60 Query: 61 ISLFGQDLEQSFASSMRKVGCMIESADFYDYVTARQYLKLNASFYPELPPQRIDEVLEAV 120 + +FG + + + +M VGC+IE + Y Y++A L+ A FYP++ QRI+EVLE Sbjct: 61 VKIFGHSILEDYEKAMAHVGCIIEIPETYPYLSAYDNLRQLARFYPDVDGQRIEEVLELT 120 Query: 121 GLAAFAKEKIRHFSTGMKQKLAMASSILHHPEFVIWDEPTNGLDIEGVVHFRALVKQLSG 180 G+ + EK + FS GMKQ+L ++++IL P+ VI DEP NGLD+EG++ R L+ L+ Sbjct: 121 GMLRYKNEKPKRFSLGMKQRLGLSAAILSRPKVVILDEPLNGLDVEGMIDIRKLILYLAE 180 Query: 181 EQGITFLISSHMIHELEQLCNRVGILQQGVLVREGNVHELLADNTSLEQYYIAELQKGKE 240 ++ TF ISSH+IH++E CN++G++ G+L+ ++L + +LE Y+++E+++ Sbjct: 181 QERTTFFISSHLIHDVELTCNKIGVIYNGMLLNVQTTEDILKNYATLENYFVSEVER--- 237 Query: 241 LLDDGRLNRS 250 +GR++ S Sbjct: 238 ---NGRVSSS 244 >emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] Length = 311 Score = 191 bits (485), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 90/218 (41%), Positives = 146/218 (66%), Gaps = 1/218 (0%) Query: 1 MESIAKLTGVSKEFHNGRGISGVNLELNMGDIYGLLGPNGAGKTTLLKMLSGLIAPSFGS 60 +E I + +SK F + +S +NL++ G+IYG LGPNGAGKTT ++ML GL+ PS G+ Sbjct: 9 LEYIVQTQNLSKNFGKEQAVSNINLQIRKGEIYGFLGPNGAGKTTTIRMLLGLMKPSSGT 68 Query: 61 ISLFGQDLEQSFASSMRKVGCMIESADFYDYVTARQYLKLNASFYPELPPQRIDEVLEAV 120 I +F +D+ + + VG ++E+ +Y ++TA + L+ +P RIDEVL+ V Sbjct: 69 IKIFQKDIVKDRIQILANVGSLVENPSYYPHLTAYENLEALRKILG-VPKTRIDEVLQTV 127 Query: 121 GLAAFAKEKIRHFSTGMKQKLAMASSILHHPEFVIWDEPTNGLDIEGVVHFRALVKQLSG 180 L A +K++ FS GMKQ+L +A+++LH+PE +I DEPTNGLD G++ R L+K+L Sbjct: 128 RLKEAANKKVKGFSLGMKQRLGIAAALLHNPELLILDEPTNGLDPSGIIEIRNLIKRLPK 187 Query: 181 EQGITFLISSHMIHELEQLCNRVGILQQGVLVREGNVH 218 E G+T +ISSH++ E++Q+ +VGI+ +G L+ + ++ Sbjct: 188 EYGMTIIISSHLLSEIDQMATQVGIVSKGTLIFQDSIE 225 >ref|NP_842835.1| bacitracin ABC transporter, ATP-binding protein, putative [Bacillus anthracis str. Ames] gb|AAP24321.1| bacitracin ABC transporter, ATP-binding protein, putative [Bacillus anthracis str. Ames] Length = 309 Score = 187 bits (475), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 93/244 (38%), Positives = 153/244 (62%) Query: 1 MESIAKLTGVSKEFHNGRGISGVNLELNMGDIYGLLGPNGAGKTTLLKMLSGLIAPSFGS 60 + +I K T ++K + + + +N+ + G+IYG +G NGAGKTT ++ML GLI P+ G+ Sbjct: 4 INTIIKTTNLTKVYGKQKSVDNLNINVQQGEIYGFIGRNGAGKTTTIRMLLGLIKPTSGT 63 Query: 61 ISLFGQDLEQSFASSMRKVGCMIESADFYDYVTARQYLKLNASFYPELPPQRIDEVLEAV 120 I +FG+DL Q+ + ++G ++E FY+ +TA++ L +NA I++ LE V Sbjct: 64 IEIFGEDLFQNQKDILSRIGSIVEVPGFYENLTAKENLLINAKIIGVYKKNAIEDTLEIV 123 Query: 121 GLAAFAKEKIRHFSTGMKQKLAMASSILHHPEFVIWDEPTNGLDIEGVVHFRALVKQLSG 180 GL K+ + +S GMKQ+L +A ++LH+PE +I DEPTNGLD G+ R L+ L+ Sbjct: 124 GLQHETKKLVGKYSLGMKQRLGIARALLHYPELLILDEPTNGLDPIGIKEMRKLIHSLAQ 183 Query: 181 EQGITFLISSHMIHELEQLCNRVGILQQGVLVREGNVHELLADNTSLEQYYIAELQKGKE 240 E+ IT LISSH++ E+EQL +R+GI+ +G L+ E ++ +L N ++ + K Sbjct: 184 ERNITILISSHILAEVEQLVDRIGIIHEGRLLEEVSLDKLRKANRKYIEFQVNNENKAAM 243 Query: 241 LLDD 244 LL++ Sbjct: 244 LLEN 247 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 362,187,743 Number of extensions: 14471201 Number of successful extensions: 105267 Number of sequences better than 1.0e-05: 17865 Number of HSP's gapped: 91607 Number of HSP's successfully gapped: 18829 Length of query: 251 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 128 Effective length of database: 423,280,401 Effective search space: 54179891328 Effective search space used: 54179891328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)