BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0032.p (206 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_821305.1| putative integral membrane protein [Streptomyce... 186 3e-46 ref|NP_105324.1| putative integral membrane protein [Mesorhizobi... 184 2e-45 ref|ZP_00107251.1| COG4705: Uncharacterized membrane-anchored pr... 180 2e-44 ref|NP_630462.1| putative integral membrane protein [Streptomyce... 177 2e-43 ref|ZP_00025521.1| COG4705: Uncharacterized membrane-anchored pr... 160 2e-38 >ref|NP_821305.1| putative integral membrane protein [Streptomyces avermitilis MA-4680] dbj|BAC67840.1| putative integral membrane protein [Streptomyces avermitilis MA-4680] Length = 276 Score = 186 bits (472), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 89/153 (58%), Positives = 108/153 (70%) Query: 2 NERQLWNKVPQVTIFFWIIKIMATTVGETAADFLNFNLNWGXXXXXXXXXXXXXISLFFQ 61 R WNKVP+VT++FW+IK++ TTVGETAAD LN G + L Q Sbjct: 20 GHRLRWNKVPEVTVYFWVIKVLCTTVGETAADLLNEKAGLGLTGVSLLMSVLLAVILVVQ 79 Query: 62 MSATKYVPSMYWLAVVLISVVGTLITDNLVDNLGVPLETTTIFFSLALAMTFVAWYASEK 121 T Y P YW AV LISVVGTL++DNL DN+G+PLETTT F++ LA+ FVAWY SE+ Sbjct: 80 FRTTAYRPGAYWTAVALISVVGTLVSDNLTDNMGIPLETTTAVFAVLLAVVFVAWYRSER 139 Query: 122 MLSIHSITTKKREAFYWLAILFTFALGTAAGDL 154 LSIHSI T +RE+FYWLA+LFTFALGT+AGDL Sbjct: 140 TLSIHSIDTLRRESFYWLAVLFTFALGTSAGDL 172 >ref|NP_105324.1| putative integral membrane protein [Mesorhizobium loti MAFF303099] dbj|BAB51110.1| putative integral membrane protein [Mesorhizobium loti] Length = 309 Score = 184 bits (466), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 93/147 (63%), Positives = 110/147 (74%) Query: 8 NKVPQVTIFFWIIKIMATTVGETAADFLNFNLNWGXXXXXXXXXXXXXISLFFQMSATKY 67 N+VP+VT+ FW+IK+MA T+GETAAD+L NL G ++L Q + +Y Sbjct: 59 NRVPEVTLDFWLIKLMAVTMGETAADYLAVNLGLGLTVTSLIMTGVLIVALGLQFAQKRY 118 Query: 68 VPSMYWLAVVLISVVGTLITDNLVDNLGVPLETTTIFFSLALAMTFVAWYASEKMLSIHS 127 VP YWLAVVLISVVGTLITDNLVD GV L+TTTI FS+ALA TF WYASE+ LSIH+ Sbjct: 119 VPWAYWLAVVLISVVGTLITDNLVDTFGVRLQTTTIAFSVALAATFAVWYASERTLSIHT 178 Query: 128 ITTKKREAFYWLAILFTFALGTAAGDL 154 I T +RE FYWLAILFTF+LGTAAGDL Sbjct: 179 IFTTRREIFYWLAILFTFSLGTAAGDL 205 >ref|ZP_00107251.1| COG4705: Uncharacterized membrane-anchored protein conserved in bacteria [Nostoc punctiforme] Length = 255 Score = 180 bits (456), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 88/150 (58%), Positives = 109/150 (72%) Query: 5 QLWNKVPQVTIFFWIIKIMATTVGETAADFLNFNLNWGXXXXXXXXXXXXXISLFFQMSA 64 ++ N+VP+VTI+FWIIK++ATTVGETAADFL+ LN G I L Q Sbjct: 3 KMLNRVPEVTIYFWIIKVLATTVGETAADFLSATLNLGLGITSYIMSAVLLIVLLNQFKL 62 Query: 65 TKYVPSMYWLAVVLISVVGTLITDNLVDNLGVPLETTTIFFSLALAMTFVAWYASEKMLS 124 +YVP+ +W VVLIS+VGTLITD LVD LGV L TTT+ FS++L + F WY+SEK L+ Sbjct: 63 KRYVPASFWFVVVLISIVGTLITDRLVDELGVSLVTTTVIFSVSLLIVFALWYSSEKTLA 122 Query: 125 IHSITTKKREAFYWLAILFTFALGTAAGDL 154 +HSI T KRE FYW+A+LFTFALGTA GDL Sbjct: 123 MHSINTAKRELFYWVAVLFTFALGTATGDL 152 >ref|NP_630462.1| putative integral membrane protein [Streptomyces coelicolor A3(2)] pir||T34982 probable integral membrane protein - Streptomyces coelicolor emb|CAA20159.1| putative integral membrane protein [Streptomyces coelicolor A3(2)] Length = 279 Score = 177 bits (448), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 88/153 (57%), Positives = 103/153 (67%) Query: 2 NERQLWNKVPQVTIFFWIIKIMATTVGETAADFLNFNLNWGXXXXXXXXXXXXXISLFFQ 61 + R NKVP+VT +FWIIK++ TTVGETAAD LN G + L Q Sbjct: 22 SHRLRRNKVPEVTAYFWIIKVLCTTVGETAADLLNEKAGLGLTGVSVLMSALLAVVLVVQ 81 Query: 62 MSATKYVPSMYWLAVVLISVVGTLITDNLVDNLGVPLETTTIFFSLALAMTFVAWYASEK 121 Y +YWLAV LISVVGTLI+DNL DN+GVPLETTT F++ LA+ FV WY E+ Sbjct: 82 FRTKAYRAGVYWLAVALISVVGTLISDNLTDNMGVPLETTTAVFAVLLAVAFVVWYRRER 141 Query: 122 MLSIHSITTKKREAFYWLAILFTFALGTAAGDL 154 LSIHSI T REA+YWLA+LFTFALGTA GDL Sbjct: 142 TLSIHSIDTTSREAYYWLAVLFTFALGTAGGDL 174 >ref|ZP_00025521.1| COG4705: Uncharacterized membrane-anchored protein conserved in bacteria [Ralstonia metallidurans] Length = 282 Score = 160 bits (404), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 84/147 (57%), Positives = 97/147 (65%) Query: 8 NKVPQVTIFFWIIKIMATTVGETAADFLNFNLNWGXXXXXXXXXXXXXISLFFQMSATKY 67 NKVP+V + FWIIKIM+TTVGET ADFL N WG +LF Q+ +Y Sbjct: 36 NKVPEVALSFWIIKIMSTTVGETGADFLAVNAGWGQGVTRTVMAALLAAALFMQLRTRRY 95 Query: 68 VPSMYWLAVVLISVVGTLITDNLVDNLGVPLETTTIFFSLALAMTFVAWYASEKMLSIHS 127 P +YWL VVL+SVVGT ITD L D LGV L +T F++ALA F WY E+ LSIH Sbjct: 96 TPWIYWLTVVLVSVVGTQITDLLTDGLGVSLYISTSAFAVALAAIFFVWYRIERTLSIHD 155 Query: 128 ITTKKREAFYWLAILFTFALGTAAGDL 154 I T+ RE FYW AIL TFALGTAAGDL Sbjct: 156 IVTRSRELFYWAAILCTFALGTAAGDL 182 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.138 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 263,457,180 Number of extensions: 8498352 Number of successful extensions: 22613 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 22566 Number of HSP's successfully gapped: 27 Length of query: 206 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 86 Effective length of database: 431,425,698 Effective search space: 37102610028 Effective search space used: 37102610028 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)