BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0157.0004.p (453 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098247.1| COG1253: Hemolysins and related proteins cont... 364 2e-99 ref|NP_348050.1| Uncharacterized CBS domain-containing protein, ... 349 8e-95 ref|NP_831812.1| Magnesium and cobalt efflux protein corC [Bacil... 346 7e-94 ref|NP_978441.1| CBS domain protein [Bacillus cereus ATCC 10987]... 344 2e-93 ref|NP_844460.1| CBS domain protein [Bacillus anthracis str. Ame... 342 1e-92 >ref|ZP_00098247.1| COG1253: Hemolysins and related proteins containing CBS domains [Desulfitobacterium hafniense] Length = 447 Score = 364 bits (935), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 193/413 (46%), Positives = 269/413 (65%), Gaps = 5/413 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 FVAAEFSLVKVR++RL +L G++ A AL V +LDAYLSA Q GITLASLGLGW+ Sbjct: 18 AFFVAAEFSLVKVRKTRLAELSESGSRKATLALAVTSQLDAYLSACQLGITLASLGLGWL 77 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEPAI+ LL PL + D L TVSVV+ F +ITFLHIVLGEL PKS+AIQ+ E + Sbjct: 78 GEPAIATLLA-PLFKNLVAWDGVLTHTVSVVIAFLLITFLHIVLGELVPKSIAIQRAESA 136 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 AL + PL FY +F P I LN AN +L+L G+ +R+L++ S + Sbjct: 137 ALWTAGPLKMFYKLFYPVIRFLNGLANLILKLGGISPANESDLAHTEEELRMLVDASQRH 196 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G++DK E KL+DN+FEFSD + EVM+PR DM LY S+EE + + + H+RYP+ Sbjct: 197 GILDKMEGKLLDNVFEFSDRVVSEVMVPRQDMMCLYIQDSMEEVLETVKTSGHTRYPLCD 256 Query: 265 EDKDRIIGFIHITDLLFAPLEQQNDLASLVRPILNVPESIEISHALRLMQKNKAQLTLVV 324 +DKD +IG +H+ DLL ++ L R IL VPE + ISH ++ M+ + L +VV Sbjct: 257 DDKDNVIGLVHMRDLLSLSESSVKNIGELKRDILIVPEGMPISHLVQKMRVQRTHLAVVV 316 Query: 325 DEYGGTAGLLTAEEILEEIVGDLHDEFED-ERPSVEHNG--DYISVEGRMLIEDVNDLTG 381 DE+GGTAG++T E+++EE+VG+++DEFE E+ + +G +Y+ + GR+L++D+++L Sbjct: 317 DEFGGTAGMVTIEDLIEELVGEIYDEFESAEQAEIIKSGESEYL-INGRVLLDDLSELLA 375 Query: 382 VVIEDDEVDSIGGWLFKELEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHR 434 V ED+ V +IGG++F L P KG R+ D TF V E RITR+ R Sbjct: 376 VEFEDETVSTIGGFVFNRLGRKPVKGDRIDFMDYTFTVMEVVGFRITRVKASR 428 >ref|NP_348050.1| Uncharacterized CBS domain-containing protein, YUGS B.subtilis ortholog [Clostridium acetobutylicum] pir||C97075 uncharacterized CBS domain-containing protein, YUGS B. subtilis ortholog [imported] - Clostridium acetobutylicum gb|AAK79390.1| Uncharacterized CBS domain-containing protein, YUGS B.subtilis ortholog [Clostridium acetobutylicum] Length = 428 Score = 349 bits (895), Expect = 8e-95, Method: Compositional matrix adjust. Identities = 182/409 (44%), Positives = 267/409 (65%), Gaps = 6/409 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 FVAAEFS+VK+R+SR+ LV +G+K A YAL V L++YLSA Q GITLASLGLGWV Sbjct: 18 AFFVAAEFSMVKIRKSRIETLVLDGDKNAKYALGVINNLNSYLSACQLGITLASLGLGWV 77 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEPA+S++L PL + + +I ++SVVVGF IIT HIVLGELAPKS+AI TE Sbjct: 78 GEPAVSKML-SPLFNLLKIPKQ-IIGSISVVVGFIIITGFHIVLGELAPKSVAILNTESI 135 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 A + PL FY P I N S + +L+L G+ IR+L+ +S K Sbjct: 136 AKATAIPLTLFYKFTYPIIVAFNKSTDLVLKLFGIKQVDEHEEAHTDEEIRMLVEESYKH 195 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G+IDK E+ +DN+F+FS+ +E+M+PRTDM ++ S ++ + + + +RYPV Sbjct: 196 GLIDKTELTFVDNVFDFSEKTVKEIMVPRTDMKCIFVEDSFDDIVGVTIDEHFTRYPVCE 255 Query: 265 EDKDRIIGFIHITDLLFAPLEQQND-LASLVRPILNVPESIEISHALRLMQKNKAQLTLV 323 E KD IIG IHI DL + ++++ + S++R I VPES+ IS R+ QK + Q+ +V Sbjct: 256 ESKDNIIGLIHIKDLYKLKINKEDEKIESIIREIKFVPESMSISELFRMFQKERMQMAIV 315 Query: 324 VDEYGGTAGLLTAEEILEEIVGDLHDEFEDERPSV--EHNGDYISVEGRMLIEDVNDLTG 381 +DEYGGT GL+T E+ILEEIVG++ DEF++E + NG Y+ V+G++LIED+N+L Sbjct: 316 IDEYGGTFGLVTIEDILEEIVGEIQDEFDEEMEEIIKTENGSYV-VDGKVLIEDINELLD 374 Query: 382 VVIEDDEVDSIGGWLFKELEGNPSKGKRVVVGDVTFEVEEATRLRITRI 430 + I ++ +D+IGGW++ +L+ P +++ G+ F + + R RI +I Sbjct: 375 IEIYEENIDTIGGWIYSKLKKYPKTNEKINYGNYQFIILKCDRRRIEKI 423 >ref|NP_831812.1| Magnesium and cobalt efflux protein corC [Bacillus cereus ATCC 14579] gb|AAP09013.1| Magnesium and cobalt efflux protein corC [Bacillus cereus ATCC 14579] Length = 435 Score = 346 bits (887), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 182/420 (43%), Positives = 273/420 (65%), Gaps = 18/420 (4%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 G FVA+EF++VKVR+SR+ QL +EGNK A A V LD YLSA Q GIT+ SLGLGW+ Sbjct: 17 GFFVASEFAVVKVRKSRIDQLANEGNKQALAARSVLSNLDVYLSACQLGITITSLGLGWL 76 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEP + +L PL +I +T T+ +T+S V+ FS+ITF H+VLGEL PKS AIQK E Sbjct: 77 GEPTVEHIL-RPLFEKINITG-TMANTLSFVIAFSVITFFHVVLGELVPKSFAIQKAEAI 134 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 L + PL+ F + PFIW+LN++A +L+G+ +R+++ +S KS Sbjct: 135 TLKFARPLILFDKIMYPFIWLLNSTAIFFTKLLGLEPAKESELAHSEEELRLILGESFKS 194 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I++ E K ++NIFEF D +A+E+M+PRT+M L + +LEENM I+ K++RYP+ Sbjct: 195 GEINQTEYKYVNNIFEFDDRVAKEIMVPRTEMICLSTENTLEENMDIVATEKYTRYPIIE 254 Query: 265 EDKDRIIGFIHITDL-------LFAPLEQQNDLASLVRPILNVPESIEISHALRLMQKNK 317 +DKD IIG I+ ++ ++ PLE S + P+L V E++ I L +QKN+ Sbjct: 255 KDKDDIIGMINTKEVFHDQTKGIYKPLE------SYIHPVLTVFETVPIRKTLVHLQKNR 308 Query: 318 AQLTLVVDEYGGTAGLLTAEEILEEIVGDLHDEFE-DERPSVEHNGDYISV-EGRMLIED 375 Q+ +V+DEYGGTAGLLT E+ILEEI+G++ DEF+ DE P +E ++V +G++LI + Sbjct: 309 VQMAIVMDEYGGTAGLLTMEDILEEIIGEIQDEFDADESPMIEKRTPKLTVLDGKVLISE 368 Query: 376 VNDLTGVVIEDDEVDSIGGWLFKE-LEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHR 434 VND+ G+ I+D ++D+IGGWL + ++ N G + F+V E ++ ++ +H+ Sbjct: 369 VNDMFGLHIDDSDLDTIGGWLLSQAVDLNIEAGYSIEYAGFQFKVLELDGHQVKKVAVHK 428 >ref|NP_978441.1| CBS domain protein [Bacillus cereus ATCC 10987] gb|AAS41049.1| CBS domain protein [Bacillus cereus ATCC 10987] Length = 435 Score = 344 bits (883), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 180/414 (43%), Positives = 269/414 (64%), Gaps = 6/414 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 G FVA+EF++VKVR+SR+ QL +EGNK A A V LD YLSA Q GIT+ SLGLGW+ Sbjct: 17 GFFVASEFAVVKVRKSRIDQLANEGNKQALAARSVISNLDVYLSACQLGITITSLGLGWL 76 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEP + LL PL +I +T T+ +T+S ++ FS+ITF H+VLGEL PKS AIQK E Sbjct: 77 GEPTVEHLL-RPLFEKINITG-TMANTLSFIIAFSVITFFHVVLGELVPKSFAIQKAEAI 134 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 L + PL+ F + PFIW+LN++A +L+G+ +R+++ +S KS Sbjct: 135 TLKFARPLILFDKIMYPFIWLLNSTAIFFTKLLGLEPAKENELAHSEEELRLILGESFKS 194 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I++ E K ++NIFEF D +A+E+M+PRT+M L + +LEENM I+ K++RYP+ Sbjct: 195 GEINQAEYKYVNNIFEFDDRVAKEIMVPRTEMICLSTENTLEENMDIVATEKYTRYPIIE 254 Query: 265 EDKDRIIGFIHITDLLFAPLEQ-QNDLASLVRPILNVPESIEISHALRLMQKNKAQLTLV 323 +DKD IIG I+ ++ + L S + P+L V E++ I L +QKN+ Q+ +V Sbjct: 255 KDKDDIIGMINTKEVFHDQTKGIHKPLESYIHPVLTVFETVPIRKTLVHLQKNRVQMAIV 314 Query: 324 VDEYGGTAGLLTAEEILEEIVGDLHDEFE-DERPSVEHNGDYISV-EGRMLIEDVNDLTG 381 +DEYGGTAGLLT E+ILEEI+G++ DEF+ DE P +E ++V +G++LI +VND+ G Sbjct: 315 MDEYGGTAGLLTMEDILEEIIGEIQDEFDADESPMIEKRTPKLTVLDGKVLISEVNDMFG 374 Query: 382 VVIEDDEVDSIGGWLFKE-LEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHR 434 + I+D ++D+IGGWL + ++ N G + F+ E ++ +I +H+ Sbjct: 375 LHIDDSDLDTIGGWLLSQAVDLNIEAGYSIEFAGFQFKALELDGHQVKKIAVHK 428 >ref|NP_844460.1| CBS domain protein [Bacillus anthracis str. Ames] gb|AAP25946.1| CBS domain protein [Bacillus anthracis str. Ames] Length = 435 Score = 342 bits (876), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 179/414 (43%), Positives = 269/414 (64%), Gaps = 6/414 (1%) Query: 25 GIFVAAEFSLVKVRQSRLTQLVSEGNKMAGYALKVNKKLDAYLSATQFGITLASLGLGWV 84 G FVA+EF++VKVR+SR+ QL +EGNK A A V LD YLSA Q GIT+ SLGLGW+ Sbjct: 17 GFFVASEFAVVKVRKSRIDQLANEGNKQALAARSVISNLDVYLSACQLGITITSLGLGWL 76 Query: 85 GEPAISELLVEPLMVQIGVTDHTLISTVSVVVGFSIITFLHIVLGELAPKSLAIQKTEGS 144 GEP + LL PL +I +T T+ +T+S ++ FS+ITF H+VLGEL PKS AIQK E Sbjct: 77 GEPTVEHLL-RPLFEKINITG-TMANTLSFIIAFSVITFFHVVLGELVPKSFAIQKAEAI 134 Query: 145 ALLLSAPLMFFYNVFLPFIWVLNASANALLRLVGVXXXXXXXXXXXXXXIRILMNQSAKS 204 L + PL+ F + PFIW+LN++A +L+G+ +R+++ +S KS Sbjct: 135 TLKFARPLILFDKIMYPFIWLLNSTAIFFTKLLGLEPAKENELAHSEEELRLILGESFKS 194 Query: 205 GVIDKDEMKLMDNIFEFSDLLAREVMLPRTDMDVLYSNLSLEENMRIITETKHSRYPVAF 264 G I++ E K ++NIFEF D +A+E+M+PRT+M L + +LEENM I+ K++RYP+ Sbjct: 195 GEINQTEYKYVNNIFEFDDRVAKEIMVPRTEMICLSTENTLEENMDIVATEKYTRYPIIE 254 Query: 265 EDKDRIIGFIHITDLLFAPLEQ-QNDLASLVRPILNVPESIEISHALRLMQKNKAQLTLV 323 +DKD IIG I+ ++ + L S + P+L V E++ I L +QKN+ Q+ +V Sbjct: 255 KDKDDIIGMINTKEVFHDQTKGIHKPLESYIHPVLTVFETVPIRKTLVHLQKNRVQMGIV 314 Query: 324 VDEYGGTAGLLTAEEILEEIVGDLHDEFE-DERPSVEHNGDYISV-EGRMLIEDVNDLTG 381 +DEYGGTAGLLT E+ILEEI+G++ DEF+ DE P +E ++V +G++LI +VND+ G Sbjct: 315 MDEYGGTAGLLTMEDILEEIIGEIQDEFDADESPMIEKRTPKLTVLDGKVLISEVNDMFG 374 Query: 382 VVIEDDEVDSIGGWLFKE-LEGNPSKGKRVVVGDVTFEVEEATRLRITRINIHR 434 + I++ ++D+IGGWL + ++ N G + F+ E ++ +I +H+ Sbjct: 375 LHIDESDLDTIGGWLLSQAVDVNIEAGYSIEYAGFQFKALELDGHQVKKIAVHK 428 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 559,254,485 Number of extensions: 22423501 Number of successful extensions: 67522 Number of sequences better than 1.0e-05: 936 Number of HSP's gapped: 65789 Number of HSP's successfully gapped: 940 Length of query: 453 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 324 Effective length of database: 406,989,807 Effective search space: 131864697468 Effective search space used: 131864697468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)