BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0154.0002.p (170 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAS55728.1| putative transposase [Aneurinibacillus thermoaero... 183 1e-45 ref|NP_243387.1| transposase (21) [Bacillus halodurans] >gi|2549... 181 4e-45 pir||S31840 probable transposase - Bacillus stearothermophilus >... 178 4e-44 ref|ZP_00089628.1| COG3335: Transposase and inactivated derivati... 67 1e-10 ref|NP_069512.1| ISA1083-1, ISORF2 [Archaeoglobus fulgidus DSM 4... 64 1e-09 >gb|AAS55728.1| putative transposase [Aneurinibacillus thermoaerophilus] Length = 172 Score = 183 bits (465), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 91/166 (54%), Positives = 118/166 (71%) Query: 3 HTASFQEIQTAMKQAKKRRMYERYQTLYLYLQGTEIEEIAHTIDRSAKTVKGYIGAYETG 62 H Q +Q AMKQ K +RMYERYQ + L+LQG + E+I+ I R+ KTV Y+ AY Sbjct: 4 HPTELQTVQQAMKQTKDKRMYERYQAISLFLQGYKYEQISAIIGRNKKTVGTYVKAYREQ 63 Query: 63 GLSGLQMNHSPGAPVRLTKEQQEKLKQTIVGSVPHDVGFTARHNWTLEIIAALIKREFGV 122 GL GL + SPGAP +LT EQ+++L IV +P DVGF AR+NWTL +I AL+++++ Sbjct: 64 GLEGLVRHASPGAPHKLTPEQEQELVDIIVNHLPVDVGFEARYNWTLALIVALVEQKWQK 123 Query: 123 TYSLRGISKMMQRQGLSYTKPTYTLAAADEEKQRFFTETTFPNLKK 168 TY+LRG+S+++ GLSYTKPTYTLA AD EKQR F E TFP LKK Sbjct: 124 TYTLRGMSRLLGSLGLSYTKPTYTLAQADPEKQRGFREETFPALKK 169 >ref|NP_243387.1| transposase (21) [Bacillus halodurans] pir||A83965 transposase (21) BH2521 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06240.1| transposase (21) [Bacillus halodurans] Length = 173 Score = 181 bits (460), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 87/159 (54%), Positives = 112/159 (70%) Query: 12 TAMKQAKKRRMYERYQTLYLYLQGTEIEEIAHTIDRSAKTVKGYIGAYETGGLSGLQMNH 71 TAMK K +RMYERYQ +Y YLQG +EI I RS KT+ Y+ AYE GL GL M Sbjct: 14 TAMKTTKDKRMYERYQAIYFYLQGYSKKEITTMIGRSEKTIYNYVNAYEEKGLDGLTMGQ 73 Query: 72 SPGAPVRLTKEQQEKLKQTIVGSVPHDVGFTARHNWTLEIIAALIKREFGVTYSLRGISK 131 SPGAP +LT EQ+++L Q I +P DVGF A++NWTL+IIA+ I++++ Y+LRG+S+ Sbjct: 74 SPGAPRKLTPEQEQELAQVIATKLPVDVGFPAKYNWTLKIIASFIEKKWNQPYTLRGVSR 133 Query: 132 MMQRQGLSYTKPTYTLAAADEEKQRFFTETTFPNLKKDI 170 ++ GLSYTKPTYTLA AD KQ+ F E TFP KK + Sbjct: 134 LLHDLGLSYTKPTYTLAKADPLKQKEFVEETFPAYKKTL 172 >pir||S31840 probable transposase - Bacillus stearothermophilus emb|CAA79750.1| putative transposase [Geobacillus stearothermophilus] Length = 160 Score = 178 bits (452), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 89/157 (56%), Positives = 114/157 (72%) Query: 2 DHTASFQEIQTAMKQAKKRRMYERYQTLYLYLQGTEIEEIAHTIDRSAKTVKGYIGAYET 61 +H +++ TAMK+AK +R YERYQ +YL+LQG EIA I RS KT+ YI AY Sbjct: 4 NHKDEIEKLSTAMKEAKSKRAYERYQAIYLHLQGYTKGEIATIIGRSKKTIYNYIHAYAQ 63 Query: 62 GGLSGLQMNHSPGAPVRLTKEQQEKLKQTIVGSVPHDVGFTARHNWTLEIIAALIKREFG 121 GL GL+M +SPGAP RLT EQ+++L I +P DVGF A++NWTL IIA LI++++G Sbjct: 64 RGLDGLEMKYSPGAPRRLTPEQEKELALIIEHQLPVDVGFEAKYNWTLAIIAELIQQKWG 123 Query: 122 VTYSLRGISKMMQRQGLSYTKPTYTLAAADEEKQRFF 158 TY+LRG S ++ R GLSYTKPTYTLA ADEEKQ+ F Sbjct: 124 PTYTLRGTSDILHRLGLSYTKPTYTLANADEEKQKEF 160 >ref|ZP_00089628.1| COG3335: Transposase and inactivated derivatives [Azotobacter vinelandii] Length = 366 Score = 67.0 bits (162), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 40/140 (28%), Positives = 66/140 (47%), Gaps = 1/140 (0%) Query: 25 RYQTLYLYLQGTEIEEIAHTIDRSAKTVKGYIGAYETGGLSGLQMNHSPGAPVRLTKEQQ 84 R Q + +G ++ +A + ++V ++ + GG + L PG P R++ E+ Sbjct: 76 RQQAIKAVREGQTVQSVAAAFGVNIRSVFRWLSDFANGGQNALLAKPVPGRPPRISAEEM 135 Query: 85 EKLKQTIVGSVPHDVGFTARHNWTLEIIAALIKREFGVTYSLRGISKMMQRQGLSYTKPT 144 L Q + P F WTL +IA LI+R+FG T SL +S++M+ G + KP Sbjct: 136 RWLAQAVRDHSPLQYRFEF-GLWTLSLIAELIRRQFGKTLSLSAVSRIMKLLGFTAQKPL 194 Query: 145 YTLAAADEEKQRFFTETTFP 164 Y D R + T+P Sbjct: 195 YRAWQQDAALVRQWESETYP 214 >ref|NP_069512.1| ISA1083-1, ISORF2 [Archaeoglobus fulgidus DSM 4304] pir||F69334 ISA1083-1, ISORF2 homolog - Archaeoglobus fulgidus gb|AAB90563.1| ISA1083-1, ISORF2 [Archaeoglobus fulgidus DSM 4304] Length = 160 Score = 64.3 bits (155), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 38/107 (35%), Positives = 57/107 (53%), Gaps = 17/107 (15%) Query: 44 TIDRSAKTVK-----GYIGA--YETGGLSGLQMNHSPGAPVRLTKEQQEKLKQTIVGSVP 96 T+ ++AK V GY+ + GL GL+ N+ G P L+ EQ+E+LK + Sbjct: 46 TVPQAAKEVGVSKVIGYVWLERWNKQGLEGLKPNYGGGRPSELSDEQKEELKSIL----- 100 Query: 97 HDVGFTARHNWTLEIIAALIKREFGVTYSLRGISKMMQRQGLSYTKP 143 R NWT + + LIK FGV YSLR +S++++ G+ Y KP Sbjct: 101 -----KERDNWTTKEVRELIKENFGVEYSLRHVSRLLKSFGMKYAKP 142 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 239,275,216 Number of extensions: 9019057 Number of successful extensions: 23174 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 23190 Number of HSP's successfully gapped: 25 Length of query: 170 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 53 Effective length of database: 439,570,995 Effective search space: 23297262735 Effective search space used: 23297262735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)