BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0031.p (457 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAS55729.1| mannose-1-phosphate guanylyltransferase [Aneurini... 599 e-170 ref|ZP_00038029.1| COG0836: Mannose-1-phosphate guanylyltransfer... 411 e-113 ref|NP_069926.1| mannose-6-phosphate isomerase/mannose-1-phospha... 194 6e-48 ref|NP_213412.1| mannose-6-phosphate isomerase/mannose-1-phospha... 193 9e-48 ref|NP_772559.1| GDP-mannose 1-phosphate guanylyltransferase [Br... 192 1e-47 >gb|AAS55729.1| mannose-1-phosphate guanylyltransferase [Aneurinibacillus thermoaerophilus] Length = 464 Score = 599 bits (1545), Expect = e-170, Method: Compositional matrix adjust. Identities = 277/450 (61%), Positives = 355/450 (78%), Gaps = 1/450 (0%) Query: 1 MKLVLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLA 60 MKL+LLSGGSGKRLWPLSNDSRSKQFLKVL++ E ESMVQRVW QL G+A+ SY+A Sbjct: 1 MKLILLSGGSGKRLWPLSNDSRSKQFLKVLKNDNNEFESMVQRVWNQLHTVGLAEDSYVA 60 Query: 61 TGRSQVEMIQSQLGAEVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPYV 120 T +SQV+M+ +QLG EVP+I+EPERRDTFPAIAL A YLYS+ GV + +++LPVDPYV Sbjct: 61 TSKSQVDMLHAQLGTEVPLIIEPERRDTFPAIALAATYLYSMEGVREDDVISVLPVDPYV 120 Query: 121 EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEA-AASGYMQVSHFQE 179 E+ FF+ V LE + +S A LAL+GV P SEKYGYI+P E + + V +F E Sbjct: 121 ESRFFDRVKDLETALVQSSAELALIGVKPTFPSEKYGYIVPKKGEHDVVANVLAVDYFVE 180 Query: 180 KPDRAQAEELISRGALWNCGVFAFRLGYLLDILQRKGLPLNYEELQKQYKLLSSISFDYE 239 KP ++A+EL+ + A WNCG+FAF+LGYL+ +L+ K LP +YEE+ Y L ISFDYE Sbjct: 181 KPSTSRAQELMKQQAFWNCGIFAFKLGYLISLLKEKKLPTDYEEMLHTYNQLPKISFDYE 240 Query: 240 VVEKEENIVVQPYAGFWKDLGTWNTLTEEMSSNHVGKGFVTADSEGTCLINELDIPVTVI 299 VVEK +NI++ PY G WKDLGTWNTLTEEM++N +GKG ++ E T LINELDIPVT++ Sbjct: 241 VVEKAQNIIMLPYEGEWKDLGTWNTLTEEMNTNLLGKGIISEGCENTHLINELDIPVTIL 300 Query: 300 GAKDLIIAASPDGILVTHKTESPRIKEVLKSFEQRPMYEERRWGHYKVIDYVKYDEGNEV 359 G + +IAASPDGILVT K SPRIK+++K+F QRPMYEERRWG Y+V+D+ K + GNEV Sbjct: 301 GISNAVIAASPDGILVTDKEASPRIKDIMKNFNQRPMYEERRWGWYRVLDHTKVENGNEV 360 Query: 360 LTKRIFISEGKNISYQLHHKRSEIWTIVSGEASIVLNEKMHNVKAGDVVRIPEGTKHAIL 419 LTKRI + GKN+SYQ+HHKRSE+WTI+SGE VLN+++++VK GDV++IP G KHAI Sbjct: 361 LTKRIGVHAGKNLSYQIHHKRSEVWTIISGEGQFVLNDQLYHVKPGDVLQIPVGAKHAIR 420 Query: 420 ALTDVEFIEVQTGSELVEEDNIRITLEWED 449 A+TD++F+EVQTGSEL+EED IR+ + WE+ Sbjct: 421 AITDLQFVEVQTGSELIEEDIIRLCMTWEE 450 >ref|ZP_00038029.1| COG0836: Mannose-1-phosphate guanylyltransferase [Enterococcus faecium] Length = 437 Score = 411 bits (1056), Expect = e-113, Method: Compositional matrix adjust. Identities = 204/447 (45%), Positives = 296/447 (66%), Gaps = 10/447 (2%) Query: 1 MKLVLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLA 60 M +VLLSGGSGKRLWPLSND RSKQF+K+ ++ G ESMVQRV+RQ+++ + +A Sbjct: 1 MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGY-ESMVQRVYRQIKKVDKDATVTIA 59 Query: 61 TGRSQVEMIQSQLGAEVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPYV 120 T ++QV I + LG +V + VEP RRDTFPAIAL AYL + GV+P E+V + PVDPYV Sbjct: 60 TSKTQVSAIHNHLGEDVCVSVEPCRRDTFPAIALATAYLVDVMGVNPEESVVVCPVDPYV 119 Query: 121 EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQVSHFQEK 180 E +FE + L + ANL LMG+ P SEKYGYIIP S + A V+ F+EK Sbjct: 120 EDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIPESAKQTAF----VNTFKEK 175 Query: 181 PDRAQAEELISRGALWNCGVFAFRLGYLLDILQRKGLPLNYEELQKQYKLLSSISFDYEV 240 P A A+E I++GALWN GVFA++L Y+++ + ++Y++L +Y+ L+ ISFDY V Sbjct: 176 PTEAVAKEYITQGALWNGGVFAYKLKYVMERAHQLIDFVDYQDLFNKYETLTKISFDYAV 235 Query: 241 VEKEENIVVQPYAGFWKDLGTWNTLTEEMSSNHVGKGFVTADSEGTCLINELDIPVTVIG 300 VE E+ I VQ +AG WKDLGTWNTLTE M + VGKG + + +G ++NEL +PV +G Sbjct: 236 VEHEDKIQVQRFAGMWKDLGTWNTLTEAMEESIVGKGVMNENCDGVHILNELAVPVLAMG 295 Query: 301 AKDLIIAASPDGILVTHKTESPRIKEVLKSFEQRPMYEERRWGHYKVIDYVKYDEGNEVL 360 D++I+ASP+GILV+ K +S IK + FEQ+ M+ E+ WG YKV+D E + Sbjct: 296 LHDVVISASPEGILVSDKEQSSYIKPFVDQFEQQIMFAEKSWGSYKVMDV-----ETESM 350 Query: 361 TKRIFISEGKNISYQLHHKRSEIWTIVSGEASIVLNEKMHNVKAGDVVRIPEGTKHAILA 420 T ++ ++ G +++Y H R E+W +++G +++ VKAGDV+ + G +H +LA Sbjct: 351 TIKVTLNAGHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQAVKAGDVITMSAGCRHTVLA 410 Query: 421 LTDVEFIEVQTGSELVEEDNIRITLEW 447 T+++ IEVQ G ++ +D + L++ Sbjct: 411 DTELKLIEVQLGKDINVKDKQKFELDY 437 >ref|NP_069926.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) [Archaeoglobus fulgidus DSM 4304] pir||H69386 mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) homolog - Archaeoglobus fulgidus gb|AAB90149.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanylyl transferase (manC) [Archaeoglobus fulgidus DSM 4304] Length = 448 Score = 194 bits (492), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 143/463 (30%), Positives = 222/463 (47%), Gaps = 47/463 (10%) Query: 1 MKLVLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLA 60 MK ++L+GG G RLWPLS + KQF+K+ ES+ Q+ ++ D Y+ Sbjct: 1 MKTLILAGGKGTRLWPLSRELMPKQFIKLFS------ESLFQKTVKRALYLSSPDEIYVI 54 Query: 61 TG---RSQVEMIQSQLGAEVP---IIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAIL 114 T R +V ++G +P II+EPE ++T PAI L AG A+L Sbjct: 55 TNKEYRFRVLDDLEEIGISIPEENIILEPEAKNTLPAICLGV----KAAGEG---KFAVL 107 Query: 115 PVDPYVEASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQV 174 P D ++A E + + + S + G+ P YGYI P + G +V Sbjct: 108 PSDHLIKADE-EYLNAFRSAEKLSENYIVTFGITPTRPHTGYGYIKPGKE---LEGGFEV 163 Query: 175 SHFQEKPDRAQAEELISRGALWNCGVFAFRLGYLLDILQRKGLPLN--YEELQKQYKLLS 232 F+EKP R AEE +S+G LWN G+F F ++ L+ EE ++ YK + Sbjct: 164 EQFKEKPSRELAEEYVSKGYLWNSGMFVFDSKVFVEELKELAPEFAKVLEEGEEAYKQIP 223 Query: 233 SISFDYEVVEKEENIVVQPYAGFWKDLGTWNTLTEEMSSNHVGKGF-----VTADSEGTC 287 SFDY ++EK + V P FW DLG ++++ E M + G + DSE Sbjct: 224 EASFDYAILEKSGRVAVVPIKTFWSDLGNFDSIYEVMEKDERGNAIKSESCIPVDSENNL 283 Query: 288 LINELDIPVTVIGAKDLIIAASPDGILVTHKTESPRIKEVLKSFEQR-------PMYEER 340 +I + +IG +DLI+ + D +LV + E+ +++EV + ++ R Sbjct: 284 VITQR--LTALIGLRDLIVIDTDDALLVARRGEAEKVREVYRLLAEKGDKAVEVHRTAHR 341 Query: 341 RWGHYKVIDYVKYDEGNEVLTKRIFISEGKNISYQLHHKRSEIWTIVSGEASIVLNEKMH 400 WG Y V++ E KRI + K +S Q H+ RSE W +V G A IV++ Sbjct: 342 PWGSYTVLE-----ENKSYKIKRITVKPKKRLSLQRHYHRSEHWVVVKGTARIVVDGNEI 396 Query: 401 NVKAGDVVRIPEGTKHAI--LALTDVEFIEVQTGSELVEEDNI 441 +++G+ +P G H I +E IE+Q G E +EED+I Sbjct: 397 LLRSGESTFVPAGAIHRIENPGKIPLEIIEIQIG-EYLEEDDI 438 >ref|NP_213412.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanyl transferase [Aquifex aeolicus] pir||H70352 mannose-6-phosphate isomerase/mannose-1-phosphate guanyl transferase - Aquifex aeolicus gb|AAC06811.1| mannose-6-phosphate isomerase/mannose-1-phosphate guanyl transferase [Aquifex aeolicus VF5] Length = 453 Score = 193 bits (490), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 135/460 (29%), Positives = 232/460 (50%), Gaps = 33/460 (7%) Query: 1 MKLVLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLA 60 MK+++LSGG G RLWPLS ++ KQF+++ S+ Q ++ ++ + + Sbjct: 1 MKILILSGGKGTRLWPLSRENFPKQFIRLFSK-----HSLFQETVKRAKKLAKDEDIVVI 55 Query: 61 TGRSQVEMIQSQL----GAEVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPV 116 TG +I+S+L EV ++VEPE R+T PAIAL YL + P ETV +LP Sbjct: 56 TGEKYEWIIRSELEEIDANEVRVVVEPEGRNTAPAIALGVKYLIH-SETPPSETVLVLPS 114 Query: 117 DPYVEAS--FFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQV 174 D ++ + F E V + E +E + L G P YGYI + G ++ Sbjct: 115 DHLIKDTEKFVEAVKKGEKYAKE--GYIVLFGEKPTYPETGYGYI--RLNGKLEEGVYEI 170 Query: 175 SHFQEKPDRAQAEELISRGA-LWNCGVFAFRLGYLL-DILQ-RKGLPLN--YEELQKQYK 229 F+EKPD +A+E +S G WNCG+F F L ++ D Q +P + +E+ K +K Sbjct: 171 EKFEEKPDYEKAKEYVSDGKHFWNCGIFLFTLDRIVKDYTQLMPEIPFHEKWEDFIKDFK 230 Query: 230 LLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNTLTEEMSSN---HVGKGFVTA-DSEG 285 ISFDY ++E+ + + V W D+G+W + + + + +V G V A D+E Sbjct: 231 NFEEISFDYAILERTKKLAVVKMDAGWSDVGSWKAVYDNLPKDEKRNVLIGDVKAMDTEC 290 Query: 286 TCLINELDIPVTVIGAKDLIIAASPDGILVTHKTESPRIKEVLKSFEQRPMYEERRWGHY 345 + L+++ + IG +D ++ + D L+ + S ++KE++K E+ +ER H Sbjct: 291 SLLLSQGKNLIACIGLEDFVVVGTEDATLIVKRDYSQKVKEIVKELEEEN--DERAIEHV 348 Query: 346 KVI----DYVKYDEGNEVLTKRIFISEGKNISYQLHHKRSEIWTIVSGEASIVLNEKMHN 401 K D G +++ + + K I ++HH R+ + ++ G A + + +K Sbjct: 349 TSFTPFGSITKLDRGERYKIRKVVVKKDKEIPLRMHHHRTVHFVVLKGTAKVKVGDKEEF 408 Query: 402 VKAGDVVRIPEGTKHAILALTDV--EFIEVQTGSELVEED 439 V + +P+ T + I+ + + E IEVQ+G L E+D Sbjct: 409 VHENESFFVPKSTPYKIINVGKIPLEMIEVQSGEYLGEDD 448 >ref|NP_772559.1| GDP-mannose 1-phosphate guanylyltransferase [Bradyrhizobium japonicum] dbj|BAC51184.1| GDP-mannose 1-phosphate guanylyltransferase [Bradyrhizobium japonicum USDA 110] Length = 472 Score = 192 bits (488), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 139/476 (29%), Positives = 214/476 (44%), Gaps = 46/476 (9%) Query: 4 VLLSGGSGKRLWPLSNDSRSKQFLKVLQSPAGEPESMVQRVWRQLQETGMADSSYLATGR 63 +++ GG+G RLWP S + R KQFL + S S Q ++ + + D + T Sbjct: 10 LIMCGGAGTRLWPASREVRPKQFLPLFGS-----RSTFQDTLLRVSDASLFDRPIVITNA 64 Query: 64 SQVEMIQSQL---GAEVPIIVEPERRDTFPAIALTAAYLYSIAGVSPRETVAILPVDPYV 120 S M+ QL G E +I+EP RRD+ PAIA A + + A V L D V Sbjct: 65 SYRFMVLEQLAEIGIEADVILEPMRRDSGPAIAAGAVFAQNRAS---EAIVLALAADHVV 121 Query: 121 EASFFETVVQLEATIQESGANLALMGVVPELASEKYGYIIPTSDEAAASGYMQVSHFQEK 180 + E S + GV PE + +YGYI + E + V+ F EK Sbjct: 122 QDKAAFVAACREGLTAASAGRIVTFGVKPERPATEYGYI--SLGEVISGEVHAVARFVEK 179 Query: 181 PDRAQAEELISRGALWNCGVFAFRLGYLLD---------------ILQRKGLPLNYEELQ 225 PD +A + ++ G LWN G F F LLD + G L + L+ Sbjct: 180 PDAVKAADYVNSGYLWNSGNFMFPATVLLDEYRKVDAASVEAVSNAVTNAGRDLGFVTLE 239 Query: 226 KQ-YKLLSSISFDYEVVEKEENIVVQPYAGFWKDLGTWNT---LTEEMSSNHVGKGFVTA 281 + + +IS DY V+EK V P + W D+G+W+ L+E+ + + G Sbjct: 240 PEAFGSAKAISIDYAVMEKTSRAAVVPVSCGWSDVGSWHAVWELSEKDAQGNAAHGAAVF 299 Query: 282 DSEGTCLINELDIPVTVIGAKDLIIAASPDGILVTHKTES-------PRIKEVLKSFEQR 334 + C + V + G DL++ A+ D +LV+ + ++ ++K V + Sbjct: 300 EDSRNCNVTTDSALVALEGVDDLVVVATADAVLVSRQKDANGLKRLVTKLKAVAPKVTEE 359 Query: 335 PMYEERRWGHYKVIDYVKYDEGNEVLTKRIFISEGKNISYQLHHKRSEIWTIVSGEASIV 394 + R WG Y+ +D G KRI + G +S Q HH R+E W +V G A + Sbjct: 360 HLKVHRPWGSYQSVD-----NGERHQVKRIVVKPGGRLSLQKHHHRAEHWIVVRGAARVT 414 Query: 395 LNEKMHNVKAGDVVRIPEGTKHAILALTDV--EFIEVQTGSELVEEDNIRITLEWE 448 +NE + V + + IP G H + + E IEVQTGS L E+D IRI +++ Sbjct: 415 VNETVKTVHENESIYIPMGAVHRMENPGKIMLELIEVQTGSYLGEDDIIRIEDDYQ 470 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 662,009,252 Number of extensions: 27199553 Number of successful extensions: 84761 Number of sequences better than 1.0e-05: 636 Number of HSP's gapped: 83156 Number of HSP's successfully gapped: 672 Length of query: 457 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 328 Effective length of database: 406,989,807 Effective search space: 133492656696 Effective search space used: 133492656696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)