BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0025.p (915 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00066007.1| hypothetical protein [Microbulbifer degradans... 105 5e-21 ref|NP_561962.1| hypothetical protein [Clostridium perfringens] ... 65 7e-09 gb|EAE94777.1| unknown [environmental sequence] 59 6e-07 gb|EAE94776.1| unknown [environmental sequence] 58 1e-06 ref|ZP_00066081.1| COG2273: Beta-glucanase/Beta-glucan synthetas... 57 2e-06 >ref|ZP_00066007.1| hypothetical protein [Microbulbifer degradans 2-40] Length = 557 Score = 105 bits (262), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 55/129 (42%), Positives = 82/129 (63%), Gaps = 4/129 (3%) Query: 786 LDRSEWKVSSNVNTQASNPANAIDGDRRTRWDTGKHQASGEYIQIDLGGVHSVEAIDLDY 845 LDRS+W VS++VN + AID TRWDT + Q +G+ ++DLG ++++ AI+LD Sbjct: 287 LDRSDWTVSASVN--GAEAEFAIDSSPNTRWDTAQSQRAGQSFEVDLGELNTLAAIELDS 344 Query: 846 TLSSYDYPRGYELYISDDARSW-NKIASGKGKLEMTKIVFPAVKTRYLRILQTGSGGNY- 903 S+ DYPRGY +Y+S+D +W + IASG T I F V R+++I QTG G+Y Sbjct: 345 AGSANDYPRGYAVYVSNDGSNWGSAIASGAAVSASTTIEFTPVSARFVKIEQTGGDGHYW 404 Query: 904 WSIQEMQVY 912 WSI + ++ Sbjct: 405 WSIHNLNIF 413 Score = 97.8 bits (242), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 53/133 (39%), Positives = 80/133 (60%), Gaps = 5/133 (3%) Query: 786 LDRSEWKVSSNVNTQASNPANAIDGDRRTRWDTGKHQASGEYIQIDLGGVHSVEAIDLDY 845 LD + W +++N A+ NAID ++ TRW TG+ Q G+ +IDL V + I LD Sbjct: 427 LDSTPWSLAANRRNSAA--GNAIDNNQSTRWTTGQTQRDGQTFEIDLSTVQTFSRIVLDS 484 Query: 846 TLSSYDYPRGYELYISDDARSW-NKIASGKGKLE-MTKIVFPAVKTRYLRILQTGS-GGN 902 S D+PR YELY+S+D +W + +A+G G +T I FP+V RY+ I Q GS + Sbjct: 485 AASDDDHPRNYELYVSNDGSNWGSPVATGAGDSSGVTVIDFPSVTARYVLIAQAGSDSSH 544 Query: 903 YWSIQEMQVYSRE 915 +WSI E+ +Y+ + Sbjct: 545 WWSIHELSIYNVQ 557 Score = 94.4 bits (233), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 58/156 (37%), Positives = 85/156 (54%), Gaps = 8/156 (5%) Query: 764 VRTAGSSVVSEKTQITAT-GLVP---LDRSEWKVSSNVNTQASNPANAIDGDRRTRWDTG 819 + +AG V+ K + + G P L+R W+VS++ + ++ A +DG TRW TG Sbjct: 121 IGSAGCDVIGPKWDLVSVYGPWPDGGLNRDAWEVSASNGSGSAQLA--LDGLASTRWATG 178 Query: 820 KHQASGEYIQIDLGGVHSVEAIDLDYTLSSYDYPRGYELYISDDARSWN-KIASGKGKLE 878 Q+ G+Y ID + ++I L T + DYPR YE+YIS + W + +G G Sbjct: 179 VFQSPGQYFDIDFQDALTFDSIVLATTENPEDYPRAYEVYISSNGSDWGVPVVTGAGNGS 238 Query: 879 MTKIVFPAVKTRYLRILQTGSGGN-YWSIQEMQVYS 913 T I V TRYLRILQTGS N +WSI E +++ Sbjct: 239 TTTIELDTVTTRYLRILQTGSSSNRWWSIHEFNIFN 274 >ref|NP_561962.1| hypothetical protein [Clostridium perfringens] dbj|BAB80752.1| hypothetical protein [Clostridium perfringens str. 13] Length = 1965 Score = 65.5 bits (158), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 58/220 (26%), Positives = 101/220 (45%), Gaps = 30/220 (13%) Query: 712 SVKLNWLEAPGASYEVLRADGSGSDLKVIATGIKQTEYTDGKLQEGTLYTYAVRTAG--- 768 S+KL W E GA+ + DG+ I IK TEY D L T Y+Y VR+ Sbjct: 886 SIKLTWDEVEGATTYDVEIDGT------IFKNIKNTEYLDTGLNYDTEYSYRVRSVNKDG 939 Query: 769 ----SSVVSEKTQITATGLVPLD-RSEWKVSSNVNTQASNPANAIDGDRRTRWDTGKHQA 823 S ++ KT + VP D ++EWK + P+ A+DGD +++ + Sbjct: 940 HSNWSELIKVKTDLDPYRNVPKDMKTEWKWGQ---YSSDEPSKAVDGDDSSQFHSKDSAI 996 Query: 824 SGEYIQIDLGGVHSVEAIDLDYTLSSYDYPRGYELYISDDARSWNKIASG---------- 873 +I ID+ +++E ++L + + + E+Y S D ++ K+ S Sbjct: 997 DKPFI-IDMQKAYTIEKLELLFRKNGNGSVKRAEIYSSLDGITYEKVFSNAEGSDIAPWT 1055 Query: 874 -KGKLEMTKIVFPAVKTRYLRILQTGSGGNYWSIQEMQVY 912 G+++ P +K RY +I+ S GN+ +++E + Y Sbjct: 1056 TDGEVKTINFNKP-IKARYFKIVTKESIGNFLAMREFRPY 1094 >gb|EAE94777.1| unknown [environmental sequence] Length = 206 Score = 58.9 bits (141), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 26/72 (36%), Positives = 45/72 (62%), Gaps = 2/72 (2%) Query: 787 DRSEWKVSSNVNTQASNPANAIDGDRRTRWDTGKHQASGEYIQIDLGGVHSVEAIDLDYT 846 DR +WK+S++ ++ +DG+ ++R+DTG Q G ++Q++L V + I LD Sbjct: 134 DRKKWKLSASHGSEKL--GGCVDGNGKSRYDTGTSQVPGMWVQVELPSVSKINRIQLDSK 191 Query: 847 LSSYDYPRGYEL 858 S+ DYPRGY++ Sbjct: 192 SSARDYPRGYQV 203 >gb|EAE94776.1| unknown [environmental sequence] Length = 75 Score = 57.8 bits (138), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 26/72 (36%), Positives = 45/72 (62%), Gaps = 2/72 (2%) Query: 787 DRSEWKVSSNVNTQASNPANAIDGDRRTRWDTGKHQASGEYIQIDLGGVHSVEAIDLDYT 846 DR +WK+S++ ++ +DG+ ++R+DTG Q G ++Q++L V + I LD Sbjct: 3 DRKKWKLSASHGSE--KLGGCVDGNGKSRYDTGTSQVPGMWVQVELPSVSKINRIQLDSK 60 Query: 847 LSSYDYPRGYEL 858 S+ DYPRGY++ Sbjct: 61 SSARDYPRGYQV 72 >ref|ZP_00066081.1| COG2273: Beta-glucanase/Beta-glucan synthetase [Microbulbifer degradans 2-40] Length = 1441 Score = 57.4 bits (137), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 58/225 (25%), Positives = 102/225 (45%), Gaps = 39/225 (17%) Query: 712 SVKLNWLEAPGASYEVLRAD---------------GSGSDLKVIATG--IKQTEYTDGKL 754 S+ L W A G+ Y++L +D G +L V ATG ++ G Sbjct: 1232 SINLTWENAYGSKYDILVSDDNVHWTLAYSEQAGDGGIDNLAVTATGQYVRLLGLERGIG 1291 Query: 755 QEGTLYTYAVRTAGSSVVSEKTQITATGLVPLDRSEWKVSSNVNTQASNP--ANAIDGDR 812 +L+++ V A VV A G K +S +++ + P ANA DG+ Sbjct: 1292 YGFSLFSFDVYGALKPVVVVPEGDLALG---------KPTSASSSEWAEPTAANATDGNT 1342 Query: 813 RTRWDTGKHQASGEYIQIDLGGVHSVEAIDLDYTLSSYDYPRGYELYISDDARSWNKI-- 870 TRW + E+I +D+G V+ V + L++ + Y +GY + S++ W + Sbjct: 1343 GTRWASA--WTDSEWISVDMGEVYPVGRVVLNWEGA---YGKGYNIQTSNNGADWTTVYT 1397 Query: 871 -ASGKGKLEMTKIVFPAVKTRYLRILQTGSGGNY-WSIQEMQVYS 913 +G G + +I+ P RY+R+L T G Y +S+ + ++Y+ Sbjct: 1398 ETAGDGGTD--EIILPFASGRYIRVLGTERGTGYGYSLWDFEIYA 1440 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.131 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,385,919,686 Number of extensions: 59750586 Number of successful extensions: 107057 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 107075 Number of HSP's successfully gapped: 12 Length of query: 915 Length of database: 757,237,578 Length adjustment: 135 Effective length of query: 780 Effective length of database: 390,699,213 Effective search space: 304745386140 Effective search space used: 304745386140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)