BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0003.p (633 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermoce... 235 2e-60 ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetas... 235 2e-60 pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridiu... 227 6e-58 gb|AAD42774.1| Man26A [Cellulomonas fimi] 169 2e-40 sp|P49424|MANA_PSEFL Mannan endo-1,4-beta-mannosidase precursor ... 131 4e-29 >emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] emb|CAA61884.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] Length = 1321 Score = 235 bits (600), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 206/353 (58%), Gaps = 13/353 (3%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKVRVAGEGATAIR 312 A GI +G+ TL+ V V+G +++ GGG SIH + K+ VVVN+ VR+ G T++ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVNKT--GVRIVTSGNTSVE 296 Query: 313 RMEIESASIIETD---ADAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 SVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 349 >ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetase [Clostridium thermocellum ATCC 27405] Length = 1321 Score = 235 bits (600), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 127/353 (35%), Positives = 206/353 (58%), Gaps = 13/353 (3%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGGEHSIHMKDAKLALVVVNRPEGKVRVAGEGATAIR 312 A GI +G+ TL+ V V+G +++ GGG SIH + K+ VVVN+ VR+ G T++ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVNKT--GVRIVTSGNTSVE 296 Query: 313 RMEIESASIIETDA---DAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 SVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 349 >pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridium thermocellum Length = 1324 Score = 227 bits (579), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 126/355 (35%), Positives = 206/355 (58%), Gaps = 15/355 (4%) Query: 18 FKKGLTTLLTTIMLVSTAAPALAYVRQDDRLST---HWAKDSIAKWQGNGVIQGYPDGSF 74 +K+ L+++L ++L+S +P V+ D ++ HWA++ + KW G++ GY DG+ Sbjct: 2 YKRLLSSVLIIMLLLSAWSPIS--VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTI 59 Query: 75 RPDNKVTRAELASMVNKLFGXXXXXXXXXXDVPAGAWYAKDLSVAKQAGYYKGFPDNKAK 134 RPDN +TRAE +++NK+FG DV WY++++ A+ AGY G+ N K Sbjct: 60 RPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKARAAGYIAGYGSNVFK 119 Query: 135 ADTQVTRQDAAALLAAVFSLEPRAGAASASFTDNAAISSYAKEAVQAL--QGTLQGYPDG 192 D +TRQ+A ++A VF L+ + S F D + + YAK++V AL +G + GY DG Sbjct: 120 PDNYITRQEAVVIIAKVFELQSGSNYTS-KFKDGSLVKEYAKDSVSALVEKGYIAGYEDG 178 Query: 193 SFRPDSPVTRAETVSIIDRLVSRYYHTAGTADGGTVQGNVLVNHTGVILKDNVISGNLYL 252 +FRPD+ +TRAET+ I+++++ Y+ G V GN L+N GVILKD VI+G+LYL Sbjct: 179 TFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINTEGVILKDTVINGDLYL 238 Query: 253 APGIGSGEATLEKVTVQGDMYIAGGG--EHSIHMKDAKLALVVVNRPEGKVRVAGEGATA 310 A GI +G+ TL+ V V+G +++ GGG +H +K K+ VVVN+ VR+ G T+ Sbjct: 239 AQGIQNGDVTLDGVNVKGTVFVNGGGSDQHYHFIKLPKINRVVVNKT--GVRIVTSGNTS 296 Query: 311 IRRMEIESASIIETD---ADAVISEVVTGAGAAGTSIVGKGTISRLAVRSGPVVL 362 + + ++S + +E D + V +AG I+ G ++ V + +L Sbjct: 297 VESVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQVDVLADDALL 351 >gb|AAD42774.1| Man26A [Cellulomonas fimi] Length = 1010 Score = 169 bits (429), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 85/176 (48%), Positives = 111/176 (63%), Gaps = 1/176 (0%) Query: 437 TEPTPRVVNLADADASSATRSLFVYLDDIRGRGTLFGQQHATDEGQSITKRDGTESDVKN 496 T P + + DADA++ TRSL YLD +RG G LFG QH T G + DGT SDVKN Sbjct: 49 TAPADETIAIVDADATAETRSLLSYLDGVRGEGILFGHQHTTSFGLTTGPTDGTTSDVKN 108 Query: 497 AVGAHPALFGWDTLSLEGKEKPGVAGDVY-ASIDNLAASMKHAYEAGGVLTLSAHMPNFV 555 G PA+FGWDTL +EG E+PG+A + +I A ++ A GGV T+SAH+ NFV Sbjct: 109 VTGDFPAVFGWDTLIIEGNERPGLAENTRDENIALFADYIRKADAIGGVNTVSAHVENFV 168 Query: 556 TGGDFYDLKGSVVSHILPGGDKHGEFNTFLDNIALFANSLKDDGGRSIPVIFRPFH 611 TGG FYD G + +LPGG H E +LD+IA A++ + D G IP++FRP+H Sbjct: 169 TGGSFYDTSGDTLRAVLPGGSHHAELVAYLDDIAELADASRRDDGTLIPIVFRPWH 224 >sp|P49424|MANA_PSEFL Mannan endo-1,4-beta-mannosidase precursor (Mannanase A) pir||S53374 mannanase A - Pseudomonas fluorescens Length = 419 Score = 131 bits (330), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 75/179 (41%), Positives = 103/179 (57%), Gaps = 19/179 (10%) Query: 441 PRVVNLADADASSATRSLFVYLDDIRGRGTLFGQQHATDEGQSITKRDGTESDVKNAVGA 500 P V L D+ A+ TRSLF ++ + R +FG QH T +G +IT+ DGT+SD NAVG Sbjct: 44 PVTVKLVDSQATMETRSLFAFMQEQRRHSIMFGHQHETTQGLTITRTDGTQSDTFNAVGD 103 Query: 501 HPALFGWDTLSLEGKEKPGVAGDVYASIDNLAASMKHAYEAGGVLTLSAHMPNFVTG--- 557 A++GWDTLS+ P GD+ A + K AY GG++T+S+H N T Sbjct: 104 FAAVYGWDTLSIVA---PKAEGDIVAQV-------KKAYARGGIITVSSHFDNPKTDTQK 153 Query: 558 -----GDFYDLKGSVVSHILPGGDKHGEFNTFLDNIALFANSLKDDGGRSIPVIFRPFH 611 G +D +VV LPGG + N +LD +A +AN+LKD+ GR IPVIFR +H Sbjct: 154 GVWPVGTSWDQTPAVVDS-LPGGAYNPVLNGYLDQVAEWANNLKDEQGRLIPVIFRLYH 211 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 893,699,493 Number of extensions: 37094385 Number of successful extensions: 74702 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 74310 Number of HSP's successfully gapped: 147 Length of query: 633 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 501 Effective length of database: 398,844,510 Effective search space: 199821099510 Effective search space used: 199821099510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)