BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0153.0001.p (183 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00116969.1| COG0262: Dihydrofolate reductase [Cytophaga h... 162 5e-39 ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga h... 135 3e-31 gb|AAS69280.1| conserved hypothetical protein [Leptospira interr... 128 6e-29 ref|NP_832899.1| Pyrimidine Reductase [Bacillus cereus ATCC 1457... 128 7e-29 ref|NP_713718.1| conserved hypothetical protein [Leptospira inte... 126 3e-28 >ref|ZP_00116969.1| COG0262: Dihydrofolate reductase [Cytophaga hutchinsonii] Length = 186 Score = 162 bits (409), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 82/182 (45%), Positives = 112/182 (61%), Gaps = 2/182 (1%) Query: 1 MGQVILMMNVTLDGCCDHTQVIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAWPAIAS 60 M ++I +N+TLDG CDHT VIADEE+HQ+ DL + +FGR YQLMES WP I Sbjct: 1 MRKLIASINMTLDGFCDHTAVIADEEMHQHVNDLFKTADTTIFGRVTYQLMESGWPPIVK 60 Query: 61 SGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GKNLVVN 119 G + + +FA +D K VFS TL ++W+NS L KG + E+ RL + GKN++V Sbjct: 61 EPTGVKAIDEFAILIDTIHKIVFSHTLKGLTWKNSTLAKGELKAEIIRLKQQPGKNILV- 119 Query: 120 GGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGVVVLRY 179 G P L L Q GL+DEY +QPI+ G+G L + DR++LK T + SG ++ RY Sbjct: 120 GSPSLIVALTQFGLIDEYQLCIQPIILGKGLILFKHMQDRIDLKCIRTETYASGAILARY 179 Query: 180 TP 181 P Sbjct: 180 EP 181 >ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga hutchinsonii] Length = 182 Score = 135 bits (341), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 71/184 (38%), Positives = 107/184 (58%), Gaps = 3/184 (1%) Query: 1 MGQVILMMNVTLDGCCDHTQVIADEELHQYTVDLMDQSAGLLFGRKIYQLMESAWPAIAS 60 M +VI +N+T+DG CDHT V D E+HQ+ DL+ + ++GR YQLM+ W +I + Sbjct: 1 MRKVIAAINMTIDGYCDHTAVNPDAEIHQHYADLLHNTGVAVYGRVTYQLMQY-WQSIRA 59 Query: 61 SGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GKNLVVN 119 + G + +FA +DR PK VFS TL W ++ L KG + EE+ L + GK++ V Sbjct: 60 NPTGKKATDEFAAAIDRVPKLVFSHTLKDTGWNSATLAKGSLEEELTALKQQPGKDIYV- 118 Query: 120 GGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGVVVLRY 179 PG+ L L+DE+ V P+VAG G L + D+ +L +T+ F SG V+L Y Sbjct: 119 CSPGMIVAAMNLNLIDEFQLCVHPVVAGSGLPLFKNIQDKPMFRLVQTKNFTSGAVILYY 178 Query: 180 TPVR 183 P++ Sbjct: 179 EPLK 182 >gb|AAS69280.1| conserved hypothetical protein [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 182 Score = 128 bits (322), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 69/188 (36%), Positives = 111/188 (59%), Gaps = 12/188 (6%) Query: 1 MGQVILMMNVTLDGCCD--HTQV---IADEELHQYTVDLMDQSAGLLFGRKIYQLMESAW 55 M ++IL ++++G + ++ + + DEE ++YT+D +DQ LLFGR Y+LM + W Sbjct: 1 MRKIILQTMLSMNGYFEGPNSTIDWHVVDEEFNKYTIDFLDQVDVLLFGRITYELMANYW 60 Query: 56 PAIASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GK 114 P + D P A K++ K VFS+TL +V W+NS L+ V +EV +L + GK Sbjct: 61 PT--AIVDNP----IIATKMNEITKIVFSKTLANVKWKNSILVYNNVKKEVAKLKKQQGK 114 Query: 115 NLVVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGV 174 ++ + G L +TL + L+DE + PIV G G L G+ +R++LKL +T+ F SG Sbjct: 115 DIAIFGSSSLAATLIEQKLIDEVRIFINPIVLGEGKTLFQGIQNRISLKLIQTKTFQSGN 174 Query: 175 VVLRYTPV 182 V+L Y P+ Sbjct: 175 VLLYYQPL 182 >ref|NP_832899.1| Pyrimidine Reductase [Bacillus cereus ATCC 14579] gb|AAP10100.1| Pyrimidine Reductase [Bacillus cereus ATCC 14579] Length = 181 Score = 128 bits (321), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 74/187 (39%), Positives = 108/187 (57%), Gaps = 12/187 (6%) Query: 1 MGQVILMMNVTLDGCCDHTQVIA---DEELHQYTVDLMDQSAGLLFGRKIYQLMESAWPA 57 MGQ+I+ M ++LDG + A +EE+ ++ DL+ +S LL GR YQ +AWP+ Sbjct: 1 MGQIIVSMYLSLDGVMEEPAWTAPYFNEEVAKFQSDLLFESEALLLGRVTYQGFAAAWPS 60 Query: 58 IASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GKNL 116 + +G FA K++ PK+V S TL+ W N+ L+KG + EE+ +L + G+NL Sbjct: 61 MTDE-EG------FADKMNSIPKFVASTTLEKTEW-NANLIKGNIVEEISKLKEQPGQNL 112 Query: 117 VVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGVVV 176 ++ G L TL Q L+DEYHF+V P++ G G L D LKL ETR SGVV+ Sbjct: 113 LIYGSGELIQTLMQHDLIDEYHFMVNPVIVGSGKHLFKTGNDTKALKLIETRTTSSGVVI 172 Query: 177 LRYTPVR 183 L Y P + Sbjct: 173 LSYQPEK 179 >ref|NP_713718.1| conserved hypothetical protein [Leptospira interrogans serovar lai str. 56601] gb|AAN50736.1| conserved hypothetical protein [Leptospira interrogans serovar lai str. 56601] Length = 182 Score = 126 bits (316), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 68/188 (36%), Positives = 111/188 (59%), Gaps = 12/188 (6%) Query: 1 MGQVILMMNVTLDGCCD--HTQV---IADEELHQYTVDLMDQSAGLLFGRKIYQLMESAW 55 M ++IL ++++G + ++ + + DEE ++YT+D +DQ LLFGR Y+LM + W Sbjct: 1 MRKIILQTMLSMNGYFEGPNSTIDWHVVDEEFNKYTIDFLDQVDVLLFGRITYELMANYW 60 Query: 56 PAIASSGDGPQFMVDFARKLDRKPKYVFSRTLDHVSWQNSFLLKGPVSEEVPRLTAE-GK 114 P + D P A K++ K VFS+TL +V W+NS L+ V +EV +L + GK Sbjct: 61 PT--AIVDNP----IIATKMNEITKIVFSKTLANVKWKNSILVYNNVKKEVAKLKKQQGK 114 Query: 115 NLVVNGGPGLGSTLAQLGLVDEYHFLVQPIVAGRGPRLLGGVLDRLNLKLAETRPFGSGV 174 ++ + G L +TL + L+DE + P+V G G L G+ +R++LKL +T+ F SG Sbjct: 115 DIAIFGSSSLVATLIEQKLIDEVRIFINPVVLGEGKTLFQGIQNRISLKLIQTKTFQSGN 174 Query: 175 VVLRYTPV 182 V+L Y P+ Sbjct: 175 VLLYYQPL 182 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 280,439,304 Number of extensions: 11798802 Number of successful extensions: 25761 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 25718 Number of HSP's successfully gapped: 35 Length of query: 183 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 65 Effective length of database: 436,855,896 Effective search space: 28395633240 Effective search space used: 28395633240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 122 (51.6 bits)