BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0008.p (442 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00160873.1| hypothetical protein Avar237701 [Anabaena var... 324 3e-87 gb|AAA93024.1| NifEN2 323 4e-87 ref|ZP_00112341.1| COG2710: Nitrogenase molybdenum-iron protein,... 306 7e-82 ref|ZP_00177001.1| hypothetical protein Cwat299801 [Crocosphaera... 300 4e-80 sp|O07356|NIFN_SYNP8 Nitrogenase iron-molybdenum cofactor biosyn... 298 2e-79 >ref|ZP_00160873.1| hypothetical protein Avar237701 [Anabaena variabilis ATCC 29413] Length = 905 Score = 324 bits (830), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 182/441 (41%), Positives = 251/441 (56%), Gaps = 12/441 (2%) Query: 3 VNRRRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQ 62 V K +VNP+K Q LGA LA G MPL HGSQGC+AF K +L RHFRE I + Sbjct: 475 VQNSHKSVAVNPLKQSQPLGAALAFLGLKGVMPLFHGSQGCTAFAKVMLVRHFREAIPLS 534 Query: 63 TSALQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSY-KRER 121 T+A+ E+ I N+E+A+ ++ K +P+IIG++ T LT+ G D + +L+S KR Sbjct: 535 TTAMTEVTTILGGEDNIEQAILTLVEKSKPEIIGLLTTGLTETRGDDMEGILRSIRKRHP 594 Query: 122 NMRGGLAFSVVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLT 181 + PDF+G+L+ GF+ AVES++ E+ Q R QI +L S T Sbjct: 595 ELYDLPIIFASTPDFQGALQDGFATAVESIVKEIPQPGETR------LDQINILVSSAFT 648 Query: 182 PADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIA 241 P D+ E+KE++S+FG E I +PD STSL GHL +S +T GG L + Q S T+A Sbjct: 649 PGDIQEIKEIVSAFGLETIVVPDHSTSLDGHLDDSYSAVTGGGTTLAELRQMGSSVFTLA 708 Query: 242 IGGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLD 301 +G SM R A L G PY+VF GL A D L L ISG VP +Y QR L D Sbjct: 709 LGESMRRAAESLQTQFGIPYEVFPQLTGLDAVDNFLQGLVDISGNAVPEKYRHQRRQLQD 768 Query: 302 SMLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGMERE-VWIG 360 +MLD HF F ALEPD L S++ +L +G E A + + ++PL+ + E V IG Sbjct: 769 AMLDTHFYFGRKRVSLALEPDLLWSIASFLASMGAEIHAAVTTTKSPLLEKLPVEKVTIG 828 Query: 361 DLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWS 420 DL+D E L G+DL I+NSHG A ++R+ +F GFP+++ LG +VGYRG + Sbjct: 829 DLEDFERLAVGSDLVIANSHGKAISRRLQTSFYRLGFPIFDRLGNGQRCTVGYRGTTQLL 888 Query: 421 NKAGNLLMQREAGHRESSVRH 441 GNL ++ E E +H Sbjct: 889 FDIGNLFLEAE----EEKAKH 905 >gb|AAA93024.1| NifEN2 Length = 905 Score = 323 bits (829), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 182/441 (41%), Positives = 251/441 (56%), Gaps = 12/441 (2%) Query: 3 VNRRRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQ 62 V K +VNP+K Q LGA LA G MPL HGSQGC+AF K +L RHFRE I + Sbjct: 475 VQNSHKSVAVNPLKQSQPLGAALAFLGLKGVMPLFHGSQGCTAFAKVMLVRHFREAIPLS 534 Query: 63 TSALQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSY-KRER 121 T+A+ E+ I N+E+A+ ++ K +P+IIG++ T LT+ G D + +L+S KR Sbjct: 535 TTAMTEVTTILGGEDNIEQAILTLVEKSKPEIIGLLTTGLTETRGDDMEGILRSIRKRHP 594 Query: 122 NMRGGLAFSVVLPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLT 181 + PDF+G+L+ GF+ AVES++ E+ Q R QI +L S T Sbjct: 595 ELYDLPIIFASTPDFQGALQDGFATAVESIVKEIPQPGETR------LDQINILVSSAFT 648 Query: 182 PADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIA 241 P D+ E+KE++S+FG E I +PD STSL GHL +S +T GG L + Q S T+A Sbjct: 649 PGDIQEIKEIVSAFGLETIVVPDHSTSLDGHLDDSYSAVTGGGTTLAELRQMGSSVFTLA 708 Query: 242 IGGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLD 301 +G SM R A L G PY+VF GL A D L L ISG VP +Y QR L D Sbjct: 709 LGESMRRAAESLQTQFGIPYEVFPQLTGLDAVDNFLQGLVDISGNAVPEKYRHQRRQLQD 768 Query: 302 SMLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGMERE-VWIG 360 +MLD HF F ALEPD L S++ +L +G E A + + ++PL+ + E V IG Sbjct: 769 AMLDTHFYFGRKRVSLALEPDLLWSIASFLASMGAEIHAAVTTTKSPLLEKLPVEKVTIG 828 Query: 361 DLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWS 420 DL+D E L G+DL I+NSHG A ++R+ +F GFP+++ LG +VGYRG + Sbjct: 829 DLEDFERLAVGSDLDIANSHGKAISRRLQTSFYRLGFPIFDRLGNGQRCTVGYRGTTQLL 888 Query: 421 NKAGNLLMQREAGHRESSVRH 441 GNL ++ E E +H Sbjct: 889 FDIGNLFLEAE----EEKAKH 905 >ref|ZP_00112341.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Nostoc punctiforme] Length = 445 Score = 306 bits (784), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 171/431 (39%), Positives = 242/431 (56%), Gaps = 8/431 (1%) Query: 3 VNRRRKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQ 62 V K +VNP+K Q+LGA LA G MPL HGSQGC+AF K +L RHFRE I + Sbjct: 4 VTASNKALTVNPLKQSQALGATLAFLGLKGTMPLFHGSQGCTAFAKVVLVRHFREAIPLA 63 Query: 63 TSALQEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERN 122 T+A+ E+ I N+E+A+ ++ K P+IIG+ T LT+ G D + LK + Sbjct: 64 TTAMTEVTTILGGEENVEQAILTLVEKANPEIIGLCSTGLTETRGDDIEGFLKEIRDRHP 123 Query: 123 MRGGLAFSVV-LPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTRQITLLPGSFLT 181 LA PDF+G+L+ GF+ AVES++ E+ + G R T Q+T+L GS T Sbjct: 124 ELNDLAIVFAPTPDFKGALQDGFAVAVESIVKEIPRAGGLR------TEQVTILAGSAFT 177 Query: 182 PADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIA 241 P DV E+KE+++SFG I +PD+ SL GHL +S +T G L + + S T+A Sbjct: 178 PGDVQEIKEIVTSFGLVPIFVPDLGASLDGHLEDNYSAVTVSGTTLKQLREVGSSAFTLA 237 Query: 242 IGGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGEPVPLRYCWQRENLLD 301 +G SM A+ L GTPY+VF GL+ DE + L +SG VP +Y QR L D Sbjct: 238 LGESMRGAAKILEERFGTPYEVFGELTGLEPVDEFIQALAILSGNSVPEKYRRQRRQLQD 297 Query: 302 SMLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGME-REVWIG 360 +MLD HF F ALEPD L S +L+ +G + A++ + + L+ + + + IG Sbjct: 298 AMLDTHFYFGAKRVSLALEPDLLWSTVHFLQSMGSQIHAVVTTTRSHLLEKLPVKSITIG 357 Query: 361 DLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWS 420 DL+D E L G+DL I NS+ A A+R++ G P+++ LG VGYRG ME Sbjct: 358 DLEDFESLAGGSDLLIGNSNVGAIAKRLSIPLYRLGLPIYDRLGNGQFTKVGYRGTMELL 417 Query: 421 NKAGNLLMQRE 431 GNL ++ E Sbjct: 418 FGIGNLFLEAE 428 >ref|ZP_00177001.1| hypothetical protein Cwat299801 [Crocosphaera watsonii WH 8501] Length = 437 Score = 300 bits (769), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 175/430 (40%), Positives = 243/430 (56%), Gaps = 15/430 (3%) Query: 19 QSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTSALQEMDVIFDANRN 78 Q LGA LA G + MPL HGSQGC+AF K +L RHFRE I + T+A+ E+ I N Sbjct: 3 QPLGAALAFLGLRKMMPLFHGSQGCTAFAKVVLVRHFRESIPLSTTAMTEVSTILGGEDN 62 Query: 79 LEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERN--MRGGLAFSVVLPDF 136 +E+A+ +L K+ P IIG++ T LT+ G D +LK Y RE++ + SV PD+ Sbjct: 63 IEKAILTILEKYNPQIIGLLTTGLTETRGDDMPRILK-YFREKHPELENFPVISVSTPDY 121 Query: 137 RGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTR-QITLLPGSFLTPADVMELKEMISSF 195 +GSL+ G++AAVE ++ V V + Q+T+L GS+++P DV E+K +I F Sbjct: 122 KGSLQDGYAAAVEGIVSADYSSVADDEPPMVGMKPQVTVLCGSYMSPGDVEEIKAVIEGF 181 Query: 196 GFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIAIGGSMERPARRLHN 255 G I +PD+S SL GHL + +T GG L + Q +S LT+AIG SM + A L Sbjct: 182 GITPIMIPDLSRSLDGHLEDDYHTVTTGGTSLTQLKQLHRSCLTLAIGESMAKAASILQE 241 Query: 256 ALGTPYKVFTHAMGLKASDELLHFLHQISG----------EPVPLRYCWQRENLLDSMLD 305 GT YK+F GL A D LL L QI VP QR L D +LD Sbjct: 242 RFGTKYKIFPRLAGLDAVDSLLWELSQILTARCDHHFPIVPNVPAHLQRQRRQLQDVILD 301 Query: 306 AHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAGMERE-VWIGDLDD 364 HF F G ALEPD L + L E+G +A + + ++P++ + E V IGDL+D Sbjct: 302 THFYFGGKKVALALEPDLLYQTAWLLTEMGANIQAAVTTTKSPILEKLPIETVTIGDLED 361 Query: 365 AEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSVGYRGAMEWSNKAG 424 E L G+DL I+NSHGI+ A+R+ + G+PV++ LG VGYRG M++ G Sbjct: 362 LEELAVGSDLIITNSHGISLAKRLKSPLYRMGYPVFDRLGLGQRCLVGYRGTMKFLFDVG 421 Query: 425 NLLMQREAGH 434 N+LM+ EA H Sbjct: 422 NILMEEEAKH 431 >sp|O07356|NIFN_SYNP8 Nitrogenase iron-molybdenum cofactor biosynthesis protein nifN gb|AAC35195.1| NifN [Cyanothece sp. PCC 8801] Length = 461 Score = 298 bits (762), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 170/444 (38%), Positives = 248/444 (55%), Gaps = 15/444 (3%) Query: 7 RKPASVNPIKTGQSLGAVLALQGCYRAMPLLHGSQGCSAFPKALLTRHFREPIAVQTSAL 66 +KP SVNP+K Q LGA LA G MPL HG+QGC+AF K +L RHFRE I + T+A+ Sbjct: 8 KKPLSVNPLKMSQPLGASLAFLGLKGMMPLFHGAQGCTAFAKVVLVRHFRESIPLSTTAM 67 Query: 67 QEMDVIFDANRNLEEALNLVLSKHRPDIIGIIGTELTDVAGVDYQSMLKSYKRERNMRGG 126 E+ I ++E+A+ ++ K++P+IIG++ T LT+ G D + +LK +++ Sbjct: 68 TEVSTILGGQDHVEQAILTIVDKNKPEIIGLLTTGLTETRGDDMEGILKDIRQKHPQLKN 127 Query: 127 LAFSVV-LPDFRGSLESGFSAAVESMIDEMIQQVGHRGSRSVNTR---QITLLPGSFLTP 182 L V PD++GSL+ ++A VE ++ +RS Q+T+L GS L+P Sbjct: 128 LPIVFVSTPDYKGSLQDCYAATVEQIVATDYNAFIAENARSAVIYPQPQVTVLAGSSLSP 187 Query: 183 ADVMELKEMISSFGFEVIALPDISTSLSGHLLTGFSPLTRGGVPLDSMLQSLQSGLTIAI 242 D+ E+K +I +FG + +PD+S SL GHL G+ +T GG L + S T+AI Sbjct: 188 GDIQEIKSIIEAFGLMPLVIPDLSRSLDGHLEDGYQSITGGGTTLPQLRSLPHSCYTLAI 247 Query: 243 GGSMERPARRLHNALGTPYKVFTHAMGLKASDELLHFLHQISGE----------PVPLRY 292 G SM A L + GT Y+VF GL+A D L L QI +P + Sbjct: 248 GESMRGAAEILKDRFGTNYEVFPRLAGLEAVDTFLWRLSQIVTSRCDHHFPFVPNIPALF 307 Query: 293 CWQRENLLDSMLDAHFQFAGMSAIAALEPDHLCSVSGWLEELGVEQKALIASYETPLVAG 352 QR L D++LD HF F G ALEPD L + L E+G + +A + + ++PL+ Sbjct: 308 ERQRRQLQDAILDTHFYFGGKKVALALEPDLLHQTAWLLTEMGAKIQAAVTTTKSPLLED 367 Query: 353 MERE-VWIGDLDDAEVLGKGADLWISNSHGIAGAQRIAAAFMPAGFPVWNELGAYMSVSV 411 + + V IGDL+D E L G DL I+NSHG A AQR+ A G+PV+++LG V Sbjct: 368 LPVDTVTIGDLEDLEDLSAGVDLIITNSHGTAMAQRLNAPLYRMGYPVFDQLGNGQRCLV 427 Query: 412 GYRGAMEWSNKAGNLLMQREAGHR 435 GYRG +++ GN+L+ EA H Sbjct: 428 GYRGTIQFLFDVGNILLAEEANHN 451 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 632,765,168 Number of extensions: 26016226 Number of successful extensions: 64224 Number of sequences better than 1.0e-05: 148 Number of HSP's gapped: 63898 Number of HSP's successfully gapped: 150 Length of query: 442 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 313 Effective length of database: 406,989,807 Effective search space: 127387809591 Effective search space used: 127387809591 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)