BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0006.p (519 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAD56231.1| dinitrogenase beta subunit [Paenibacillus durus] 654 0.0 sp|Q57118|NIFK_FRAAL NITROGENASE MOLYBDENUM-IRON PROTEIN BETA CH... 546 e-154 sp|O07643|NIFK_CYAA5 Nitrogenase molybdenum-iron protein beta ch... 544 e-153 gb|AAD17261.1| NifK [Frankia sp. EuIK1] 540 e-152 ref|ZP_00160872.1| hypothetical protein Avar237501 [Anabaena var... 539 e-152 >emb|CAD56231.1| dinitrogenase beta subunit [Paenibacillus durus] Length = 387 Score = 654 bits (1686), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/375 (82%), Positives = 340/375 (90%), Gaps = 1/375 (0%) Query: 1 MSRDRLEIPDYNSLFSEERYVQQRANKKQFEAPCSEQDTAEALAYSKSAEYMQKNFDRKA 60 MS++R+ IPDYN+LF+EERYVQQR NKKQFEAPCS+Q+ AEALAYS+SAEYM+KNF+RKA Sbjct: 1 MSKERMNIPDYNTLFTEERYVQQRENKKQFEAPCSDQEKAEALAYSQSAEYMEKNFERKA 60 Query: 61 VVINPHKACQPLGSVMAALGFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTED 120 VVINPHKACQPLGSVMAAL FEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTED Sbjct: 61 VVINPHKACQPLGSVMAALIFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTED 120 Query: 121 AAVFGGMNNLIDGLENSIALYKPEMIALCTTCMAEVIGDDLSSFIGNARIKGAITEDFPV 180 AAVFGGM+N+IDGLEN++ALYKPEM+A+CTTCMAEVIGDDLS+FIGNAR KGAI EDFPV Sbjct: 121 AAVFGGMSNMIDGLENAVALYKPEMVAVCTTCMAEVIGDDLSAFIGNARQKGAIAEDFPV 180 Query: 181 AFCNTPSFVGSHITGYDSMLKGILSYLYERSGVQANPGSGEEAGEKLNVMLGFEPYTGNF 240 F NTPSF+GSHITGYDSM+K ILS L++RSG A PG+GEE GEKLNV+LGFEPYTGNF Sbjct: 181 TFANTPSFIGSHITGYDSMIKSILSQLFDRSGQTAAPGAGEE-GEKLNVLLGFEPYTGNF 239 Query: 241 AEIRKILEAFDTKYTLLGDHSGNYDSPATXXXXXXXXXTKLADVPLAANALGTLSLQKYT 300 AEIRKIL AFDTKYT+LGDHSGNYDSPA TKLADVP AANALG+L LQKYT Sbjct: 240 AEIRKILNAFDTKYTILGDHSGNYDSPANGEYEYYYGGTKLADVPKAANALGSLVLQKYT 299 Query: 301 LKKTQDFISGTWNQQLTALSTPLGITGTDKLLDAVSELTGLPVPASLMEERGRVVDALTD 360 LKKTQ++I GTW QQ++ALSTPLG+ TDKLL+A+SELTGLPVPASL EERGRVVDA D Sbjct: 300 LKKTQEYIKGTWKQQVSALSTPLGVKATDKLLEAISELTGLPVPASLKEERGRVVDAYAD 359 Query: 361 SHPYLHGKRVALVGD 375 SHPYLHGKRVAL GD Sbjct: 360 SHPYLHGKRVALAGD 374 >sp|Q57118|NIFK_FRAAL NITROGENASE MOLYBDENUM-IRON PROTEIN BETA CHAIN (NITROGENASE COMPONENT I) (DINITROGENASE) gb|AAB53116.1| nitrogenase beta subunit gb|AAA96264.1| dinitrogenase beta subunit Length = 528 Score = 546 bits (1407), Expect = e-154, Method: Compositional matrix adjust. Identities = 263/514 (51%), Positives = 352/514 (68%), Gaps = 6/514 (1%) Query: 6 LEIPDYNSLFSEERYVQQRANKKQFEAPCSEQDTAEALAYSKSAEYMQKNFDRKAVVINP 65 L + D+N +F +E Y +Q K++FE +++ L +++ EY +KNF R+A+ +NP Sbjct: 13 LRVLDHNDIFRDEVYQKQFEGKREFENAAPKEEVQRVLDWTRGWEYREKNFAREALTVNP 72 Query: 66 HKACQPLGSVMAALGFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTEDAAVFG 125 KACQPLG+V+AALGFE T+P VHGSQGC +YFRSH +RHFKEP PA S+SMTEDAAVFG Sbjct: 73 AKACQPLGAVLAALGFEGTMPLVHGSQGCVAYFRSHFARHFKEPVPAASTSMTEDAAVFG 132 Query: 126 GMNNLIDGLENSIALYKPEMIALCTTCMAEVIGDDLSSFIGNARIKGAITEDFPVAFCNT 185 G+NNL++ +EN ALYKP+ IA+ TTCMAEVIG+DL ++IG AR + AIT+DFPV++ +T Sbjct: 133 GINNLVEAMENITALYKPKHIAVSTTCMAEVIGEDLFAYIGAAREQEAITQDFPVSYAHT 192 Query: 186 PSFVGSHITGYDSMLKGILSYLYERSGVQANPGSGEEAGEKLNVMLGFEPYTGNFAEIRK 245 P FVGSHITGYD MLKGIL L + A+ + + +LNV+ GF+ Y GN E ++ Sbjct: 193 PCFVGSHITGYDVMLKGILEMLAKSPDADASKVAKSDK-VRLNVIPGFDTYIGNHREYKR 251 Query: 246 ILEAFDTKYTLLGDHSGNYDSPATXXXXXXXXXTKLADVPLAANALGTLSLQKYTLKKTQ 305 ILE +LGDHS + DSPA T LA+ LA + +L LQ+ TL++T Sbjct: 252 ILELMGVDPLILGDHSSSLDSPADGEYDLYPGGTPLAEGALAKFSRASLVLQESTLRRTA 311 Query: 306 DFISGTWNQQLTALSTPLGITGTDKLLDAVSELTGLPVPASLMEERGRVVDALTDSHPYL 365 ++I W Q+ L TPLG+ GTD L AV+++ + VPA L ERGR+VDA+TDSH Y+ Sbjct: 312 EYIEKDWKQETVLLETPLGVKGTDAFLTAVAKVAEVEVPAELTAERGRLVDAITDSHAYV 371 Query: 366 HGKRVALVGDPDLLIGLIGFCLEIGMEPVHIVCSNGDVDFNEVKFKDEAEALLASSPYGS 425 HGKRVA+ GDPDL+I L F LE+GM PVHIV +N D FK E +L++S +G Sbjct: 372 HGKRVAIAGDPDLVIALTRFVLELGMIPVHIVSTNAD-----TTFKARMEKVLSASKFGE 426 Query: 426 EAALYIGNDLWHMRSLLINDPVDLAIGSSHLKFAAKDAGVPLVRVGFPILDRHHMHRYPI 485 EA ++ DLWH+RSL+ +PVDL IGS LK+ +++A VPLVRVGFPI DRHH+HR+PI Sbjct: 427 EATVWPEKDLWHLRSLVFTEPVDLLIGSMQLKYISREANVPLVRVGFPIFDRHHLHRFPI 486 Query: 486 IGYQGXXXXXXXXXXXXXDELDRNNSGFNYDLVR 519 IGY G DE+DRN+ +++L R Sbjct: 487 IGYTGGLHLLTQLVNTILDEMDRNDPDHSFELRR 520 >sp|O07643|NIFK_CYAA5 Nitrogenase molybdenum-iron protein beta chain (Nitrogenase component I) (Dinitrogenase) gb|AAB61284.1| dinitrogenase beta-subunit [Cyanothece sp. ATCC 51142] Length = 511 Score = 544 bits (1401), Expect = e-153, Method: Compositional matrix adjust. Identities = 266/524 (50%), Positives = 349/524 (66%), Gaps = 18/524 (3%) Query: 1 MSRDRLEIPDYNSLFSEERYVQQRANKKQFEAPCSEQDTAEALAYSKSAEYMQKNFDRKA 60 M+++ I D+ LF + Y + NKKQFE + + E ++KS EY +KNF R+A Sbjct: 1 MAQNVNNIKDHVDLFHQPEYQELFENKKQFEGMPTAEKVQEVAEWTKSWEYREKNFAREA 60 Query: 61 VVINPHKACQPLGSVMAALGFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTED 120 + +NP KACQPLG+++AA+GFE TLPFVHGSQGC +YFR+HL+RHFKEP AVSSSMTE+ Sbjct: 61 LTVNPAKACQPLGALLAAIGFEGTLPFVHGSQGCVAYFRTHLTRHFKEPVSAVSSSMTEN 120 Query: 121 AAVFGGMNNLIDGLENSIALYKPEMIALCTTCMAEVIGDDLSSFIGNARIKGAITEDFPV 180 AAVFGG+ N++DGL+NS ALYKP+MIA+CTTCMAEVIGDDL +F+GNAR G I +D PV Sbjct: 121 AAVFGGLKNMVDGLQNSYALYKPKMIAVCTTCMAEVIGDDLGAFLGNARQDGVIPDDLPV 180 Query: 181 AFCNTPSFVGSHITGYDSMLKGILSYLYERSGVQANPGSGEEAGEKLNVMLGFEPYTGNF 240 F +TPSFVGSHITGYDSM+K ILS L E + G K+N + GFE Y GN Sbjct: 181 PFAHTPSFVGSHITGYDSMMKSILSTLTEGKKKETTNG-------KINFIAGFETYIGNV 233 Query: 241 AEIRKILEAFDTKYTLLGDHSGNYDSPATXXXXXXXXXTKLADVPLAANALGTLSLQKYT 300 I+ I+ AFD + TLL D DSP T L D + NA T++LQ YT Sbjct: 234 RAIKNIISAFDLEGTLLSDTEMYLDSPNLGEFKMYHEGTSLEDAADSINAEATVTLQTYT 293 Query: 301 LKKTQDFISGTWNQQLTALSTPLGITGTDKLLDAVSELTGLPVPASLMEERGRVVDALTD 360 KT+++I W Q+ T P G+ GTD+ L +SELTG P+P RGR VDA+TD Sbjct: 294 TPKTREYIEKKWGQK-TYTYRPWGVKGTDEFLMGLSELTGKPIPKEFEIARGRAVDAMTD 352 Query: 361 SHPYLHGKRVALVGDPDLLIGLIGFCLEIGMEPVHIVCSNGDVDFNEVKFKDEAEALLAS 420 + ++HGKR A+ GDPDL++GL+ F LE+G+EPVH++ +N +F E EA+ALLA+ Sbjct: 353 TQAWVHGKRAAVYGDPDLVMGLLQFMLEMGIEPVHVLVNNSTKEFEE-----EAKALLAA 407 Query: 421 SPYGSEAALYIGNDLWHMRSLLINDPVDLAIGSSHLKFAAKDAGVPLVRVGFPILDRHHM 480 SPYG +A ++ G DLWHMRSLL +PVD +G+S+ K+ +D PL+R+G+PI DRHH+ Sbjct: 408 SPYGQQATVWGGKDLWHMRSLLFTEPVDFLVGNSYAKYLQRDTKTPLIRIGYPIFDRHHL 467 Query: 481 HRYPIIGYQGXXXXXXXXXXXXXDELDRNN-----SGFNYDLVR 519 HRY IGY+G DELDR + ++DLVR Sbjct: 468 HRYSTIGYEGAINLLNWIANGLMDELDRKTDTPSVTDISFDLVR 511 >gb|AAD17261.1| NifK [Frankia sp. EuIK1] Length = 517 Score = 540 bits (1391), Expect = e-152, Method: Compositional matrix adjust. Identities = 263/514 (51%), Positives = 346/514 (67%), Gaps = 9/514 (1%) Query: 6 LEIPDYNSLFSEERYVQQRANKKQFEAPCSEQDTAEALAYSKSAEYMQKNFDRKAVVINP 65 + D++ LF + Y +Q K +FE S + A L +++ EY +KNF R+A+ +NP Sbjct: 13 FRVLDHSELFKDVVYQKQFEGKAEFENGSSSAEVARVLEWTRGWEYREKNFAREALTVNP 72 Query: 66 HKACQPLGSVMAALGFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTEDAAVFG 125 KACQPLG+V+A LGF+ TLP VHGSQGC +YFRSH +RHFKEP PA S+SMTEDAAVFG Sbjct: 73 AKACQPLGAVLAGLGFQGTLPLVHGSQGCVAYFRSHFARHFKEPVPAASTSMTEDAAVFG 132 Query: 126 GMNNLIDGLENSIALYKPEMIALCTTCMAEVIGDDLSSFIGNARIKGAITEDFPVAFCNT 185 G+NNL++ LEN+ LYKP+M+A+ TTCMAEVIG+DL ++IG A+ KGAI+ D+PV + +T Sbjct: 133 GLNNLVEALENATRLYKPKMVAVSTTCMAEVIGEDLFAYIGAAKEKGAISTDYPVPYAHT 192 Query: 186 PSFVGSHITGYDSMLKGILSYLYERSGVQANPGSGEEAGEKLNVMLGFEPYTGNFAEIRK 245 PSFVGSHITGYDSMLKGIL L +S + P +G + +LN++ GFE YTGN E R+ Sbjct: 193 PSFVGSHITGYDSMLKGILRNL-TKSADRTEPAAGGKP--RLNIIPGFETYTGNLREYRR 249 Query: 246 ILEAFDTKYTLLGDHSGNYDSPATXXXXXXXXXTKLADVPLAANALGTLSLQKYTLKKTQ 305 +LE +LGDHS + DSPA T LAD A + T+ LQ+ +KT Sbjct: 250 VLELMGVDPLILGDHSDSLDSPADGEYDLYPGGTPLADAAKAKFSRATVLLQESATRKTT 309 Query: 306 DFISGTWNQQLTALSTPLGITGTDKLLDAVSELTGLPVPASLMEERGRVVDALTDSHPYL 365 + I W Q L TP+G GTD+ L +S L G+ +PA L ERGR+VDALTDSH YL Sbjct: 310 ELIRDVWKQDTLVLETPIGPGGTDQFLTEISRLAGVEIPAELTVERGRLVDALTDSHAYL 369 Query: 366 HGKRVALVGDPDLLIGLIGFCLEIGMEPVHIVCSNGDVDFNEVKFKDEAEALLASSPYGS 425 HGKRVA+ GDPDL++ L F LE+GM PVH++ +N D FK E +L++S +G Sbjct: 370 HGKRVAIAGDPDLVVALTRFVLELGMIPVHVLSTNAD-----TSFKARMEKVLSASKFGE 424 Query: 426 EAALYIGNDLWHMRSLLINDPVDLAIGSSHLKFAAKDAGVPLVRVGFPILDRHHMHRYPI 485 A ++ DLWH+RSL+ +PVDL IGS++LK+ A++A VPLVRVGFPI DRHH+HR+PI Sbjct: 425 AATVWPEKDLWHLRSLVFTEPVDLLIGSTYLKYIAREANVPLVRVGFPIFDRHHLHRFPI 484 Query: 486 IGYQGXXXXXXXXXXXXXDELDRNNSGFNYDLVR 519 IGY G DEL+ + +YD VR Sbjct: 485 IGYTGRAALVTQLVNTVLDELE-TAADHSYDAVR 517 >ref|ZP_00160872.1| hypothetical protein Avar237501 [Anabaena variabilis ATCC 29413] Length = 512 Score = 539 bits (1388), Expect = e-152, Method: Compositional matrix adjust. Identities = 272/518 (52%), Positives = 344/518 (66%), Gaps = 18/518 (3%) Query: 7 EIPDYNSLFSEERYVQQRANKKQFEAPCSEQDTAEALAYSKSAEYMQKNFDRKAVVINPH 66 +I D+ LF ++ Y + KK+FE + ++ ++K+ EY +KNF R+A+ +NP Sbjct: 8 KIKDHAELFQQQEYQELFQAKKEFECGHNPEEVTRIAEWTKTWEYREKNFARQALTVNPA 67 Query: 67 KACQPLGSVMAALGFEKTLPFVHGSQGCNSYFRSHLSRHFKEPTPAVSSSMTEDAAVFGG 126 KACQPLGS++AA+GFE TLPFVHGSQGC +YFRSHL+RHFKEP AVSSSMTEDAAVFGG Sbjct: 68 KACQPLGSILAAVGFEGTLPFVHGSQGCVAYFRSHLTRHFKEPFSAVSSSMTEDAAVFGG 127 Query: 127 MNNLIDGLENSIALYKPEMIALCTTCMAEVIGDDLSSFIGNARIKGAITEDFPVAFCNTP 186 + NL++GL S LYKP+MIA+CTTCMAEVIGDDL +FI A+ +GA+ EDFPV + +TP Sbjct: 128 LKNLVEGLAVSYNLYKPKMIAVCTTCMAEVIGDDLQAFIRTAKEEGAVPEDFPVPYAHTP 187 Query: 187 SFVGSHITGYDSMLKGILSYLYERSGVQANPGSGEEAGEKLNVMLGFEPYTGNFAEIRKI 246 SFVGSHI GYD+M+KGILS L R + G K+N + GFE Y GN EI++I Sbjct: 188 SFVGSHILGYDNMMKGILSNLTARKKKETTNG-------KINFIPGFETYIGNLREIKRI 240 Query: 247 LEAFDTKYTLLGDHSGNYDSPATXXXXXXXXXTKLADVPLAANALGTLSLQKYTLKKTQD 306 YTLL D+S DSP TKL D + NA T++LQ Y KT++ Sbjct: 241 ANVMGINYTLLADNSEYLDSPNNGEYNMYPGGTKLEDAADSINAEATIALQAYATTKTRE 300 Query: 307 FISGTWNQQLTALSTPLGITGTDKLLDAVSELTGLPVPASLMEERGRVVDALTDSHPYLH 366 +I W Q T ++ P+GI GTD+ L +S LTG P+P L +ERGR VDALTDS +LH Sbjct: 301 YIEHEW-QHKTYVNRPVGIRGTDEFLMKLSALTGKPIPQELEDERGRAVDALTDSQAWLH 359 Query: 367 GKRVALVGDPDLLIGLIGFCLEIGMEPVHIVCSNGDVDFNEVKFKDEAEALLASSPYGSE 426 GKRVA+ GDPDL+IGL F LE+G EPVHIV SN NE F+ E ALL SSP+G Sbjct: 360 GKRVAMYGDPDLVIGLTQFLLEVGAEPVHIVVSNS----NE-HFEAELRALLDSSPFGQG 414 Query: 427 AALYIGNDLWHMRSLLINDPVDLAIGSSHLKFAAKDAGVPLVRVGFPILDRHHMHRYPII 486 A ++ G DLWHMRSLL +PVDL IG+S+ K+ +D P VR+G+PI DRHH+HRY Sbjct: 415 ATIHGGRDLWHMRSLLFTEPVDLLIGNSYGKYLWRDTKTPFVRIGYPIFDRHHLHRYATY 474 Query: 487 GYQGXXXXXXXXXXXXXDELDRN-----NSGFNYDLVR 519 GYQG DELDRN + +YDL+R Sbjct: 475 GYQGTINLLNWIVNTILDELDRNTIIPSKTDISYDLIR 512 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 755,289,079 Number of extensions: 31468745 Number of successful extensions: 67653 Number of sequences better than 1.0e-05: 171 Number of HSP's gapped: 67249 Number of HSP's successfully gapped: 176 Length of query: 519 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 389 Effective length of database: 404,274,708 Effective search space: 157262861412 Effective search space used: 157262861412 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)