BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0005.p (488 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAD56230.1| dinitrogenase alpha subunit [Paenibacillus durus] 917 0.0 ref|ZP_00014414.1| COG2710: Nitrogenase molybdenum-iron protein,... 688 0.0 ref|NP_949953.1| nitrogenase molybdenum-iron protein alpha chain... 682 0.0 gb|AAM63412.2| dinitrogenase alpha-subunit [Frankia sp. Mrp182] 678 0.0 pir||A42624 nitrogenase (EC 1.18.6.1) molybdenum-iron protein al... 677 0.0 >emb|CAD56230.1| dinitrogenase alpha subunit [Paenibacillus durus] Length = 485 Score = 917 bits (2370), Expect = 0.0, Method: Compositional matrix adjust. Identities = 433/488 (88%), Positives = 466/488 (95%), Gaps = 3/488 (0%) Query: 1 MGLNIEENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPR 60 MGL+IE NKK+VE++LEAYP KAKKDR+KHFQINTEE + +C+LKSNIKSRPGVMTPR Sbjct: 1 MGLDIEANKKLVEDILEAYPDKAKKDRKKHFQINTEETKQSCSCSLKSNIKSRPGVMTPR 60 Query: 61 GCSFAGSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSD 120 GCS+AGSKGVVWGPIKDMVHISHGP+G GQ SWGTRRNYANGTLGIDNFTAMQITSD Sbjct: 61 GCSYAGSKGVVWGPIKDMVHISHGPVGCGQY---SWGTRRNYANGTLGIDNFTAMQITSD 117 Query: 121 FQETDIVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMP 180 FQETDIVFGGDKKLE+I REI EMFPLAKGISVQSECP+GLIGDDIEAVSKKMAKEL+MP Sbjct: 118 FQETDIVFGGDKKLEIICREIKEMFPLAKGISVQSECPIGLIGDDIEAVSKKMAKELEMP 177 Query: 181 IVPVRCEGFRGVSQSLGHHIANDAIRDFVLGKAELAETGPYDVNIIGDYNIGGDAWASRI 240 +VPVRCEGFRGVSQSLGHHIANDAIRDFV+GKA+LAETGPYDVNIIGDYNIGGDAWASRI Sbjct: 178 VVPVRCEGFRGVSQSLGHHIANDAIRDFVMGKADLAETGPYDVNIIGDYNIGGDAWASRI 237 Query: 241 LLEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFF 300 LLEEMGLRVIAQWSGDGTLNELEIAHKAK+NLIHCHRSMNYMVEHMEKAY IPWMEYNFF Sbjct: 238 LLEEMGLRVIAQWSGDGTLNELEIAHKAKMNLIHCHRSMNYMVEHMEKAYGIPWMEYNFF 297 Query: 301 GPSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRS 360 GP+KTYESLRAIAA+FDATIQ+NCEK+IAK+KP MDAII KY+PRLE KKV+L+IGGLRS Sbjct: 298 GPTKTYESLRAIAAKFDATIQENCEKVIAKYKPQMDAIIGKYRPRLEGKKVMLLIGGLRS 357 Query: 361 RHTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDL 420 RHTIGAYEDLGM+IVA+GYEFAHKDDYEKTFP MKEG+IIMDDPTAYELEELAQKLN+DL Sbjct: 358 RHTIGAYEDLGMEIVATGYEFAHKDDYEKTFPDMKEGSIIMDDPTAYELEELAQKLNIDL 417 Query: 421 VGSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLIRKKEK 480 +GSGVKEKYVYHKMG+PFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVN+PVW+LI KKEK Sbjct: 418 MGSGVKEKYVYHKMGIPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNNPVWSLIDKKEK 477 Query: 481 AQKEGAGV 488 Q EGA V Sbjct: 478 VQPEGASV 485 >ref|ZP_00014414.1| COG2710: Nitrogenase molybdenum-iron protein, alpha and beta chains [Rhodospirillum rubrum] Length = 488 Score = 688 bits (1776), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/475 (68%), Positives = 386/475 (81%), Gaps = 6/475 (1%) Query: 4 NIEENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCS 63 + E ++K +EEVLEAYP+KA K R+KH E + +C +KSN+K+ PGVMT RGC+ Sbjct: 8 DAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEG-SSCGVKSNVKAIPGVMTIRGCA 66 Query: 64 FAGSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQE 123 +AGSKGVVWGP+KDMVHISHGP+G GQ SW RRNY G +G+D+F MQ TSDFQE Sbjct: 67 YAGSKGVVWGPVKDMVHISHGPVGCGQY---SWSQRRNYFTGQVGVDSFVTMQFTSDFQE 123 Query: 124 TDIVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVP 183 DIVFGGDKKLE +I EI +FPL +GIS+QSECP+GLIGDDIEAV++K AK++ +PI+P Sbjct: 124 KDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIGLIGDDIEAVARKKAKDVGLPIIP 183 Query: 184 VRCEGFRGVSQSLGHHIANDAIRDFVLGK-AELA-ETGPYDVNIIGDYNIGGDAWASRIL 241 VRCEGFRGVSQSLGHHIANDAIRD+V + +E A ET PYDVNIIGDYNIGGDAWASRIL Sbjct: 184 VRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWASRIL 243 Query: 242 LEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFFG 301 LEEMGLRVIAQWSGD T+ E+E A KAKLNLIHC+RSMNY+ HME+ + +PWMEYNFFG Sbjct: 244 LEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYNFFG 303 Query: 302 PSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRSR 361 PS+ +SLRAIAA FD TIQ+ E++IA H+P +DA+INKYK RLE K+V+L +GGLR R Sbjct: 304 PSQIEKSLRAIAANFDETIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGLRPR 363 Query: 362 HTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDLV 421 H + AYEDLGM I +GYEFAH DDY++T KEGT+I DD T YELE +KL DLV Sbjct: 364 HVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGTLIYDDLTGYELERFIEKLRPDLV 423 Query: 422 GSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLIR 476 GSG+KEKY KMGVPFRQMHSWDYSGPYHG+DGF IFA+DMDM +N+PVW L++ Sbjct: 424 GSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLK 478 >ref|NP_949953.1| nitrogenase molybdenum-iron protein alpha chain, nifD [Rhodopseudomonas palustris CGA009] emb|CAE30059.1| nitrogenase molybdenum-iron protein alpha chain, nifD [Rhodopseudomonas palustris CGA009] Length = 487 Score = 682 bits (1761), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/476 (68%), Positives = 385/476 (80%), Gaps = 11/476 (2%) Query: 6 EENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCSFA 65 E NKK++ EVLEAYP K+ K R KH +NT +A+ C++KSNIKS PGVMT RGC++A Sbjct: 14 ERNKKLIGEVLEAYPDKSAKRRAKH--LNTYDAEKA-ECSVKSNIKSIPGVMTIRGCAYA 70 Query: 66 GSKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQETD 125 GSKGVVWGPIKDMVHISHGP+G GQ SWG+RRNY GT G+D F MQ TSDFQE D Sbjct: 71 GSKGVVWGPIKDMVHISHGPVGCGQY---SWGSRRNYYKGTTGVDTFGTMQFTSDFQEKD 127 Query: 126 IVFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELD-MPIVPV 184 IVFGGDKKL II EI E+FPL+KGISVQSECP+GLIGDDIEAVSK +K+ D PI+PV Sbjct: 128 IVFGGDKKLGKIIDEIQELFPLSKGISVQSECPIGLIGDDIEAVSKAKSKQYDGKPIIPV 187 Query: 185 RCEGFRGVSQSLGHHIANDAIRDFVLGKAELAETG----PYDVNIIGDYNIGGDAWASRI 240 RCEGFRGVSQSLGHHIAND IRD+V KA G PYDV IIGDYNIGGDAWASRI Sbjct: 188 RCEGFRGVSQSLGHHIANDVIRDWVFDKAGEKNAGFQSTPYDVAIIGDYNIGGDAWASRI 247 Query: 241 LLEEMGLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFF 300 LLEEMGLRVIAQWSGDGT+ ELE KAKLN++HC+RSMNY+ HME+ + IPW+EYNFF Sbjct: 248 LLEEMGLRVIAQWSGDGTIAELENTPKAKLNILHCYRSMNYITRHMEEKFGIPWVEYNFF 307 Query: 301 GPSKTYESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRS 360 GP+K SLR IA++FD I++ E++IAK+KP M+A+I KY+PRLE KKV+L +GGLR Sbjct: 308 GPTKIEASLREIASKFDDKIKEGAERVIAKYKPQMEAVIAKYRPRLEGKKVMLYVGGLRP 367 Query: 361 RHTIGAYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDL 420 RH IGAYEDLGM++V +GYEF H DDY++T +K+GT+I DD T YE E+ +K+ DL Sbjct: 368 RHVIGAYEDLGMEVVGTGYEFGHNDDYQRTTHYVKDGTLIYDDVTGYEFEKFVEKVRPDL 427 Query: 421 VGSGVKEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLIR 476 VGSGVKEKY++ KMGVPFRQMHSWDYSGPYHG+DGF IFA+DMD+ +N+PVW L + Sbjct: 428 VGSGVKEKYIFQKMGVPFRQMHSWDYSGPYHGYDGFGIFARDMDIAINAPVWKLTK 483 >gb|AAM63412.2| dinitrogenase alpha-subunit [Frankia sp. Mrp182] Length = 486 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/470 (67%), Positives = 378/470 (80%), Gaps = 6/470 (1%) Query: 7 ENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCSFAG 66 E + ++ EVL YPKKA K R KH + N E C +KSNIKSRPGVMT RGC++AG Sbjct: 11 ETEAMIAEVLSQYPKKAAKFRAKHLKANDPEGSK--ECEVKSNIKSRPGVMTIRGCAYAG 68 Query: 67 SKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQETDI 126 SKGVVWGP+KDMV ISHGP+G GQ SW TRRNYA+G LG+DNFTAMQIT+DFQE DI Sbjct: 69 SKGVVWGPVKDMVTISHGPVGCGQY---SWATRRNYAHGHLGVDNFTAMQITTDFQEKDI 125 Query: 127 VFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVPVRC 186 VFGGD KLE + EI E+FPLAKGISVQSECP+GLIGDDIEAV+KK AK+LD+P++PVRC Sbjct: 126 VFGGDPKLEQVCDEIVELFPLAKGISVQSECPIGLIGDDIEAVAKKSAKKLDIPVIPVRC 185 Query: 187 EGFRGVSQSLGHHIANDAIRDFVLGKA-ELAETGPYDVNIIGDYNIGGDAWASRILLEEM 245 EGFRGVSQSLGHHIANDA+RD VLG + E YDV +IGDYNIGGDAWASR +LEEM Sbjct: 186 EGFRGVSQSLGHHIANDAVRDHVLGTGGDSFERTDYDVALIGDYNIGGDAWASRRILEEM 245 Query: 246 GLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFFGPSKT 305 GLRVIAQWSGDGT+NE+ H +KLNLIHC+RSMNY+ ME+ + PW E+NFFGP+K Sbjct: 246 GLRVIAQWSGDGTINEMASTHLSKLNLIHCYRSMNYICTTMEERFGTPWTEFNFFGPTKI 305 Query: 306 YESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRSRHTIG 365 S+R IA FD I+ E IA+++ D I ++PRLE K+V+L +GGLR RHTIG Sbjct: 306 ISSMRKIAEFFDDEIKAKTEAAIARYQVRFDEITKAFRPRLEGKRVMLAVGGLRPRHTIG 365 Query: 366 AYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDLVGSGV 425 AYEDLGM++V +GYEFAHKDDY +T+ M+KEGT++ DDPTA+ELEE A+ L DL+G+GV Sbjct: 366 AYEDLGMEVVGTGYEFAHKDDYTRTYGMLKEGTVLYDDPTAFELEEFAKFLKPDLMGAGV 425 Query: 426 KEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLI 475 KEKYV+HKMG+PFRQMHSWDYSGPYHG DGF +FA+DMD+ +NSP W+L+ Sbjct: 426 KEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPAWDLL 475 >pir||A42624 nitrogenase (EC 1.18.6.1) molybdenum-iron protein alpha chain - Frankia alni (strain ArI3) gb|AAA96263.1| dinitrogenase alpha subunit Length = 486 Score = 677 bits (1747), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/470 (66%), Positives = 378/470 (80%), Gaps = 6/470 (1%) Query: 7 ENKKIVEEVLEAYPKKAKKDREKHFQINTEEAQSCGTCALKSNIKSRPGVMTPRGCSFAG 66 E + ++ EVL YPKKA K R KH + N E C +KSNIKSRPGVMT RGC++AG Sbjct: 11 ETEAMIAEVLSQYPKKAAKFRAKHLKANDPEGSK--ECEVKSNIKSRPGVMTIRGCAYAG 68 Query: 67 SKGVVWGPIKDMVHISHGPIGWGQXXXXSWGTRRNYANGTLGIDNFTAMQITSDFQETDI 126 SKGVVWGP+KDMV ISHGP+G GQ SW TRRNYA+G LG+DNFTAMQIT+DFQE DI Sbjct: 69 SKGVVWGPVKDMVTISHGPVGCGQY---SWATRRNYAHGHLGVDNFTAMQITTDFQEKDI 125 Query: 127 VFGGDKKLEVIIREITEMFPLAKGISVQSECPVGLIGDDIEAVSKKMAKELDMPIVPVRC 186 VFGGD KLE + EI E+FPLAKGISVQSECP+GLIGDDIEAV+KK +K+LD+P++PVRC Sbjct: 126 VFGGDPKLEQVCDEIVELFPLAKGISVQSECPIGLIGDDIEAVAKKSSKKLDIPVIPVRC 185 Query: 187 EGFRGVSQSLGHHIANDAIRDFVLGKA-ELAETGPYDVNIIGDYNIGGDAWASRILLEEM 245 EGFRGVSQSLGHHIANDA+RD VLG + E YDV +IGDYNIGGDAWASR +LEEM Sbjct: 186 EGFRGVSQSLGHHIANDAVRDHVLGTGGDSFEQTDYDVALIGDYNIGGDAWASRRILEEM 245 Query: 246 GLRVIAQWSGDGTLNELEIAHKAKLNLIHCHRSMNYMVEHMEKAYDIPWMEYNFFGPSKT 305 GLRVIAQWSGDGT+NE+ H +KLNLIHC+RSMNY+ ME+ + PW E+NFFGP+K Sbjct: 246 GLRVIAQWSGDGTINEMASTHLSKLNLIHCYRSMNYICTTMEERFGTPWTEFNFFGPTKI 305 Query: 306 YESLRAIAARFDATIQQNCEKMIAKHKPAMDAIINKYKPRLENKKVLLMIGGLRSRHTIG 365 S+R IA FD I+ E IA+++ D I ++PRLE K+V+L +GGLR RHTIG Sbjct: 306 ISSMRKIAEFFDDEIKAKTEAAIARYQVRFDEITKAFRPRLEGKRVMLAVGGLRPRHTIG 365 Query: 366 AYEDLGMDIVASGYEFAHKDDYEKTFPMMKEGTIIMDDPTAYELEELAQKLNVDLVGSGV 425 AYEDLGM++V +GYEFAHKDDY +T+ M+KEGT++ DDPTA+ELEE A+ L DL+G+GV Sbjct: 366 AYEDLGMEVVGTGYEFAHKDDYTRTYSMLKEGTVLYDDPTAFELEEFAKFLKPDLMGAGV 425 Query: 426 KEKYVYHKMGVPFRQMHSWDYSGPYHGFDGFKIFAKDMDMTVNSPVWNLI 475 KEKYV+HKMG+PFRQMHSWDYSGPYHG DGF +FA+DMD+ +NSP W+L+ Sbjct: 426 KEKYVFHKMGIPFRQMHSWDYSGPYHGVDGFAVFARDMDIAINSPAWDLL 475 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 757,606,111 Number of extensions: 32754125 Number of successful extensions: 79421 Number of sequences better than 1.0e-05: 310 Number of HSP's gapped: 78505 Number of HSP's successfully gapped: 320 Length of query: 488 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 358 Effective length of database: 404,274,708 Effective search space: 144730345464 Effective search space used: 144730345464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)