BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0003.p (456 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC27790.1| NifB2 protein [Paenibacillus durus] 773 0.0 emb|CAD56228.1| NifB1 protein [Paenibacillus durus] 530 e-149 ref|ZP_00112314.1| COG0535: Predicted Fe-S oxidoreductases [Nost... 437 e-121 gb|AAD17269.1| NifB [Frankia sp. EuIK1] 434 e-120 ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena var... 433 e-120 >emb|CAC27790.1| NifB2 protein [Paenibacillus durus] Length = 458 Score = 773 bits (1995), Expect = 0.0, Method: Compositional matrix adjust. Identities = 373/452 (82%), Positives = 409/452 (90%), Gaps = 3/452 (0%) Query: 7 CISSEAEEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVSEV 66 C +S AE++ISRHPCYSEEAHRF+ARMHIPVAPACNIQC+YCNRKFDCVNESRPGVVSEV Sbjct: 8 CSTSAAEDDISRHPCYSEEAHRFFARMHIPVAPACNIQCNYCNRKFDCVNESRPGVVSEV 67 Query: 67 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLSTNG 126 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLAN ++TFDTFARVKK+VPDV CLSTNG Sbjct: 68 LTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANADKTFDTFARVKKHVPDVITCLSTNG 127 Query: 127 LTLYRHIDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRG 186 LTLYRHID IVELGI HVTITINAID DVG+ IYPWV DEGVRYEG+ AA LLISRQL+G Sbjct: 128 LTLYRHIDRIVELGIGHVTITINAIDPDVGKEIYPWVSDEGVRYEGREAAALLISRQLQG 187 Query: 187 LELLAKLGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQYEKDGRK 246 +E LAK GILVKVNSI+IPG+ND HL VSK+VKELGATLHNVTPLIIAPGSQYEKDGRK Sbjct: 188 VEELAKRGILVKVNSILIPGINDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRK 247 Query: 247 APRPKELHNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPVINAEAR 306 APRP+EL+ LQE L +GG K+MRHCRQCRADA+GLLGQDRN+DF LE MEADPVI+ +AR Sbjct: 248 APRPRELNQLQEQLSEGGTKLMRHCRQCRADAVGLLGQDRNQDFQLETMEADPVIDLDAR 307 Query: 307 ALFQGELDVKIRERVQAKEAR--LLRENAPKIRVAVATRGGDKVNQHFGHATEFLVYDTD 364 FQ ELD KIR+R+ AKE R +L EN K RVAVA+RGGDKVNQHFGHATEF+++DTD Sbjct: 308 EQFQQELDSKIRQRLIAKENRRSILDENK-KTRVAVASRGGDKVNQHFGHATEFMIFDTD 366 Query: 365 GAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLNKAG 424 GAEV+ +G+RKIQAYCHGTADCNGDK TLQEI SI+SDCRILL SGIGD+PRA+LNKAG Sbjct: 367 GAEVKFIGIRKIQAYCHGTADCNGDKGETLQEITSILSDCRILLSSGIGDAPRAALNKAG 426 Query: 425 VLPLVRKGGIQEAILESVKYSSYFENINISKG 456 VLPLVRKGGIQEAILESVKY SYFENI ISKG Sbjct: 427 VLPLVRKGGIQEAILESVKYGSYFENITISKG 458 >emb|CAD56228.1| NifB1 protein [Paenibacillus durus] Length = 320 Score = 530 bits (1365), Expect = e-149, Method: Compositional matrix adjust. Identities = 259/321 (80%), Positives = 285/321 (88%), Gaps = 3/321 (0%) Query: 138 ELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRGLELLAKLGILV 197 ELGI HVTITINAID DVG+ IYPWVFDEGVRYEG+ AA LLISRQL+G+E LAK GILV Sbjct: 1 ELGIGHVTITINAIDPDVGKEIYPWVFDEGVRYEGREAAALLISRQLQGVEELAKRGILV 60 Query: 198 KVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAPGSQYEKDGRKAPRPKELHNLQ 257 KVNSIMIPGVND HL VSK+VKELGATLHNVTPLIIAPGSQYEKDGRKAPRP+EL+ LQ Sbjct: 61 KVNSIMIPGVNDKHLVEVSKKVKELGATLHNVTPLIIAPGSQYEKDGRKAPRPRELNQLQ 120 Query: 258 ELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADPVINAEARALFQGELDVKI 317 E L +GG K+MRHCRQCRADA+GLLGQDRN+DF LE MEADPVI+ +AR FQ ELD+KI Sbjct: 121 EQLSEGGTKLMRHCRQCRADAVGLLGQDRNQDFQLETMEADPVIDLDAREQFQQELDIKI 180 Query: 318 RERVQAKEAR--LLRENAPKIRVAVATRGGDKVNQHFGHATEFLVYDTDGAEVQLLGVRK 375 R+R+ AKE R +L EN K RVAVA+RGGDKVNQHFGHATEF+++DTDG EV+ +G+RK Sbjct: 181 RQRLIAKENRRTILDEN-KKTRVAVASRGGDKVNQHFGHATEFMIFDTDGTEVKFIGIRK 239 Query: 376 IQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLNKAGVLPLVRKGGIQ 435 IQAYCHGTADCNGDK TLQEI SI+SDCRILL SGIGD+PRA LNKAGVLPLVRKGGIQ Sbjct: 240 IQAYCHGTADCNGDKDETLQEITSILSDCRILLSSGIGDAPRAVLNKAGVLPLVRKGGIQ 299 Query: 436 EAILESVKYSSYFENINISKG 456 EAILESVKY SYFENINISKG Sbjct: 300 EAILESVKYGSYFENINISKG 320 >ref|ZP_00112314.1| COG0535: Predicted Fe-S oxidoreductases [Nostoc punctiforme] Length = 495 Score = 437 bits (1123), Expect = e-121, Method: Compositional matrix adjust. Identities = 220/446 (49%), Positives = 298/446 (66%), Gaps = 15/446 (3%) Query: 13 EEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVSEVLTPEQA 72 +E I++HPCYSE+AH YARMH+ VAPACNIQC+YCNRK+DC NESRPGVVSE+LTPE+A Sbjct: 54 QERIAKHPCYSEDAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEA 113 Query: 73 ERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLSTNGLTLYRH 132 K + ++ Q++VVGIAGPGDPLANPE+TF TF + PD+ LCLSTNGL L + Sbjct: 114 AHKALVIGGKIPQMTVVGIAGPGDPLANPEKTFRTFELIAAQAPDIKLCLSTNGLMLPDY 173 Query: 133 IDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRGLELLAK 192 ID I +L I HVTITIN +D ++G IYPWV RY G A++L RQ+ GL+ L Sbjct: 174 IDRIKQLNIDHVTITINMVDPEIGAKIYPWVHYRRKRYRGLEGAKILHERQMEGLQALKD 233 Query: 193 LGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAP--GSQYEKDGRKAPRP 250 IL KVNS+MIPG+NDHHL V++ ++E GA LHN+ PLI AP G+ + G++ P P Sbjct: 234 ADILCKVNSVMIPGINDHHLVEVNRVIREKGAFLHNIMPLISAPEHGTHFGLIGQRGPTP 293 Query: 251 KELHNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEA-MEADPVINAEARALF 309 KEL +Q+ G MK+MRHCRQCRADA+GLLG+DR+++F E +E P + E RA Sbjct: 294 KELKEVQDNCS-GNMKMMRHCRQCRADAVGLLGEDRSQEFSKEKFLEMAPEYDMEQRATV 352 Query: 310 QGELDVKIRERVQAKEARLLR----ENAPKIRVAVATRGGDKVNQHFGHATEFLVYDTDG 365 ++ K +E ++ + + L N+PK+ VAVAT+GG VNQHFGHA EF +Y+ G Sbjct: 353 HEGIE-KFKEELKVAKDKALAGKKIANSPKVLVAVATKGGGLVNQHFGHAKEFQIYEVGG 411 Query: 366 AEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLNKAGV 425 EV+ +G RKI YC G + A+ + I+ ++DC+ +L S IG+ P+ L +AG+ Sbjct: 412 NEVRFIGHRKIDQYCQGGY----GEEASFEHIMKAIADCKAVLVSKIGNCPQEKLQEAGI 467 Query: 426 LPLVRKGGIQEAILESVKYSSYFENI 451 + I++ LE Y Y + + Sbjct: 468 KTVEAYDVIEKVALEF--YEQYVKEL 491 >gb|AAD17269.1| NifB [Frankia sp. EuIK1] Length = 658 Score = 434 bits (1117), Expect = e-120, Method: Compositional matrix adjust. Identities = 221/434 (50%), Positives = 292/434 (67%), Gaps = 11/434 (2%) Query: 5 SPCISSEAEEEISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVS 64 +P E E+I+ HPCYS+EAH++YARMH+ VAP CNIQC+YCNRK+DC NESRPGV S Sbjct: 37 TPATDPEIAEKIANHPCYSQEAHQYYARMHVAVAPGCNIQCNYCNRKYDCANESRPGVTS 96 Query: 65 EVLTPEQAERKVKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLST 124 ++LTPE A KVK VA+++ Q+SV+GIAGPGDPLANP++TF T V ++ PD+ LCLST Sbjct: 97 DLLTPEDALAKVKLVASEIKQMSVLGIAGPGDPLANPKRTFRTLELVARDCPDIKLCLST 156 Query: 125 NGLTLYRHIDEIVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQL 184 NGLTL H+D I L + HVTITIN ID +VG+ IYPWV +G +Y G+ +++L RQL Sbjct: 157 NGLTLPDHVDRIAALNVDHVTITINMIDPEVGELIYPWVAYKGKKYTGRERSKILSERQL 216 Query: 185 RGLELLAKLGILVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAP--GSQYEK 242 GL +LA+ IL KVNS+MIPGVND HL VSK V+ LGA LHNV PL+ AP G+ + Sbjct: 217 EGLAMLAERKILAKVNSVMIPGVNDEHLVEVSKTVRGLGAFLHNVMPLVSAPEHGTVFGL 276 Query: 243 DGRKAPRPKELHNLQELL-GQGG--MKVMRHCRQCRADAIGLLGQDRNRDFPLEAMEADP 299 G+ P P++L LQ+ G+ G M +MRHCRQCRADA+GLLG+DR +F E + Sbjct: 277 AGQPGPTPQQLKALQDRCEGEDGADMNMMRHCRQCRADAVGLLGEDRGDEFTPETYQGRE 336 Query: 300 V-INAEARALFQGELDVKIRERVQAKEARLLRENAPK----IRVAVATRGGDKVNQHFGH 354 + + E R E++ +E +E + A K + VAVAT+G VNQHFGH Sbjct: 337 IAYDLEGRQAAHPEIERWRQETRAERETVTVAGPATKPDEVVLVAVATKGSGVVNQHFGH 396 Query: 355 ATEFLVYDTDGAEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGD 414 ATEF +Y+ +L+ R +Q YC G +C GD A+ L + I ++SDC +LCS IG Sbjct: 397 ATEFWIYEAGRNWARLVQTRDVQRYCEGPTEC-GDGASVLDKTIEMLSDCSAVLCSKIGP 455 Query: 415 SPRASLNKAGVLPL 428 P +L AG+ P+ Sbjct: 456 GPYDALEAAGIEPV 469 >ref|ZP_00160867.1| hypothetical protein Avar237001 [Anabaena variabilis ATCC 29413] gb|AAA93017.1| NifB2 Length = 484 Score = 433 bits (1114), Expect = e-120, Method: Compositional matrix adjust. Identities = 221/435 (50%), Positives = 290/435 (66%), Gaps = 16/435 (3%) Query: 16 ISRHPCYSEEAHRFYARMHIPVAPACNIQCHYCNRKFDCVNESRPGVVSEVLTPEQAERK 75 I +HPCYSEEAH YARMH+ VAPACNIQC+YCNRK+DC NESRPGVVSE+LTPE+A K Sbjct: 45 IEKHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSELLTPEEAAHK 104 Query: 76 VKGVAAQLMQLSVVGIAGPGDPLANPEQTFDTFARVKKNVPDVSLCLSTNGLTLYRHIDE 135 V +A ++ Q++V+GIAGPGDPLANPE+TF TF + + PD+ LCLSTNGL L ++D Sbjct: 105 VLVIAGKIPQMTVLGIAGPGDPLANPEKTFRTFELIAEKAPDIKLCLSTNGLMLPDYVDR 164 Query: 136 IVELGIRHVTITINAIDADVGQAIYPWVFDEGVRYEGKAAAELLISRQLRGLELLAKLGI 195 I +L I HVTITIN +D ++G IYPWV RY+G AA++L +Q+ GL+ L + I Sbjct: 165 IKQLNIDHVTITINMVDPEIGTKIYPWVHYRRKRYKGIEAAQILHEKQMEGLQALQEADI 224 Query: 196 LVKVNSIMIPGVNDHHLPAVSKRVKELGATLHNVTPLIIAP--GSQYEKDGRKAPRPKEL 253 L KVNS+MIPG+ND HL V++ ++ GA LHN+ PLI AP G+ + G++ P KEL Sbjct: 225 LCKVNSVMIPGINDEHLVEVNQVIRSKGAFLHNIMPLISAPEHGTHFGLTGQRGPTAKEL 284 Query: 254 HNLQELLGQGGMKVMRHCRQCRADAIGLLGQDRNRDFPLEA-MEADPVINAEARALF--- 309 +Q+ G MK+MRHCRQCRADA+GLLG+DR+++F E ME P N E R Sbjct: 285 KQIQDNCA-GNMKMMRHCRQCRADAVGLLGEDRSQEFTKEKFMEMAPEYNLEQRQTVHAG 343 Query: 310 ----QGELDVKIRERVQAKEARLLRENAPKIRVAVATRGGDKVNQHFGHATEFLVYDTDG 365 Q E+ V ++E+V N+PKI VAVAT+GG VNQHFGHA EF++Y+ DG Sbjct: 344 IEKSQKEIQV-VKEKVVETLQTTSFNNSPKILVAVATKGGGLVNQHFGHAKEFMIYEVDG 402 Query: 366 AEVQLLGVRKIQAYCHGTADCNGDKAATLQEIISIVSDCRILLCSGIGDSPRASLNKAGV 425 + + RKI YC + ATL II +SDC+ +L S IG+ P+ L KAG+ Sbjct: 403 KSAKFVSHRKIDHYCQS----GYGEEATLDNIIHAISDCQAVLVSKIGNCPQEQLLKAGL 458 Query: 426 LPLVRKGGIQEAILE 440 + I++ LE Sbjct: 459 QTVEAYDVIEKVALE 473 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 660,094,177 Number of extensions: 27034131 Number of successful extensions: 63075 Number of sequences better than 1.0e-05: 100 Number of HSP's gapped: 62959 Number of HSP's successfully gapped: 100 Length of query: 456 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 327 Effective length of database: 406,989,807 Effective search space: 133085666889 Effective search space used: 133085666889 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)