BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0151.0001.p (792 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098440.1| COG0085: DNA-directed RNA polymerase, beta su... 109 3e-22 ref|NP_622513.1| conserved hypothetical protein [Thermoanaerobac... 105 3e-21 ref|ZP_00061389.1| hypothetical protein [Clostridium thermocellu... 90 2e-16 ref|ZP_00100195.1| COG3468: Type V secretory pathway, adhesin Ai... 61 9e-08 emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermoce... 60 2e-07 >ref|ZP_00098440.1| COG0085: DNA-directed RNA polymerase, beta subunit/140 kD subunit [Desulfitobacterium hafniense] Length = 742 Score = 109 bits (272), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 168/701 (23%), Positives = 299/701 (42%), Gaps = 85/701 (12%) Query: 114 WNISWSY--EIGGTSYGFSTQVDALTGDLINTYITYPFLQQDAYYPPKLTRADALEQARK 171 W+++W+ + GG+ F+ QVD TG++I+ P PK + A+A + A+ Sbjct: 87 WSLNWNSTDDAGGS---FNAQVDVSTGEIIHMNSWNPASSSSQTSVPKYSYAEAKDIAQG 143 Query: 172 FIAKAAPSLGPDDIQLS---DNEAMNPVGGSLFGTVQYNFYFKLLKNGLPSASDVLSVTV 228 + K LG Q+ +N + PV +G Y+ +K + NG+ S+ +SV V Sbjct: 144 LVNKI---LGDRLNQMELVPENTQITPVID--YGYTTYSVQWKRVANGVAFPSNGVSVQV 198 Query: 229 NG-NGDVLQFSKTAPGPEYPSAKPAISLESAQKKFTDGFAVGLYYIPVY--------KDN 279 N +G + +S P K IS + A++ F + L Y Y + + Sbjct: 199 NAYDGTITSYSLNWSKESIPDPKDVISADKAREAFVKNNLLQLQYFLNYGIRPLDSQEKD 258 Query: 280 TIEKWVLGWRPQEQSLYS-INAQTGKSIDNEGAE----ASALPITYEAVPQAKERFQAAG 334 + +L ++ QS I+AQTG+ + E + + AK A Sbjct: 259 AKKDALLVYKLSNQSFSGMIDAQTGEPLKLNSGEWLNSDAVMEAGGGMGGMAKSSENALA 318 Query: 335 TGKEITAAEAAKLVQQVVAIPAERK----PDNQSL-----GTGYPNTKQKVWRLSWRDNS 385 ++ +A+KL+ + AI + +K PD+ +L G ++++VW W Sbjct: 319 PAEQKEVDDASKLLTKEQAIESAKKWVEIPDSLTLRGANLGLEGGLSQKRVWSFDWSQEE 378 Query: 386 NTRMAAFPASSYAEVDAATGQIYQYQLEQFTGSKDVKPQPAPAGGKKLTQAEAKQQAILL 445 +T + YA VDAATG++ G P P P G + + AK A Sbjct: 379 STDGYNY---VYARVDAATGEVL--------GFSAYSPNPQPKGPDAIDRTAAKAIADAF 427 Query: 446 VNRLY-DQASSV--LKLAEHGGTWSVLPDGKGFRFQFVRYYNGIPVSDTGVTLAMDIYG- 501 + ++ D+ V L++ ++G + D F + R NG+ V G +++++ Sbjct: 428 IKKIQPDRVQQVEYLEVMDNGQKYP--EDQPNHDFSYERVVNGVHVPGNGFSVSVERNTQ 485 Query: 502 RLESYSA--ANPDFTTIQGQPTPAISQSEAMQSYKDRYAMKLQYNRIGGYNNIGYVEPQV 559 ++ SY+ AN +F I A+ Q + ++ + ++L+Y +I + + Sbjct: 486 KVVSYNMDFANVNFPDI----AQAMDQKQGEDTFLKKRPLELKYVQINKNGQLS----DI 537 Query: 560 KLVYSPLPVNEQNQYEVLDAATGKWVKVNAYSGQNAGTPAAV-DLKGHPAEQALTELVKY 618 +LVY P N ++DA TG+++ + P D+ G AE+ + L + Sbjct: 538 RLVYQPKMDNSFAVSNIMDAKTGEFLDWQGKPIKEQPRPYNFGDISGSFAEEEIRLLGQA 597 Query: 619 GVLVPDADGKINPDQEITVGDWFT--YIAAASTSSYQGYTNGTEQKPVAGVNPDSEYYKA 676 G D + PD+ +TV + IA SYQG + D E K Sbjct: 598 GAFGEYGD-QFRPDEHVTVKSLLSSMLIAKNGVWSYQGLS-------------DEELLKQ 643 Query: 677 VSFAANHEWISKDTVLQPDNKLSREQLAVQLASFLQYSKISVFLENDTAVSSFSDSASIK 736 V W+ ++ L ++++SRE A + LQ KI+ E A +D+ S Sbjct: 644 VKELG---WVKENLSL--NDQVSREFQAKLVVRMLQLEKIAQLEELFQAPYEDADTFSDG 698 Query: 737 NKGAVALAVKLGLMQGDNGKFNPQQNVTKAGVATVLMKLVE 777 + G +ALA LG+M + KF + +T+A A L+K ++ Sbjct: 699 SLGYIALAKGLGVMNIEGNKFESTKKMTRAEAAYALVKALQ 739 >ref|NP_622513.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24117.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 702 Score = 105 bits (263), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 178/740 (24%), Positives = 303/740 (40%), Gaps = 99/740 (13%) Query: 69 SKAKITEDQAVAKTRELFPVLKDATVSGVRLGEDNSWPPSSNQMIWNISWSYEIGGTSYG 128 +K + E +AKT+ +SG +S+ N +WN++W+ GT Sbjct: 30 TKISLKEAIEIAKTK--------LGISGSGYDFTSSYSEYVNYKVWNLTWNSTTKGT--- 78 Query: 129 FSTQVDALTGDLINTYITYPFLQQDAYYP--PKLTRADALEQARKFIAKAAPSLGPDDIQ 186 S V+A TG++++ P + Y P PK TR +A + A +F+ K P Q Sbjct: 79 ISVSVNANTGEIVSYSTWTP--TSEPYKPVIPKYTREEARKVAIEFLQKITPEKFK---Q 133 Query: 187 LSDNEAMNPVGGSLFGTVQYNFYFKLLKNGLPSASDVLSVTVNGNG-DVLQFSKTAPGPE 245 + P+G + Y+F F+ + NG+ ++ L V V+ N V + + Sbjct: 134 TKEIVPSYPLGA--YYDANYSFSFERIVNGISFPNNGLYVYVDKNTLKVTSYFQNWEDYS 191 Query: 246 YPSAKPAISLESAQKKFTDGFAVGLYYIPVYKDNTIEKWVLGWRPQEQSLYSINAQTGKS 305 +P + I+ E A K F + + L Y + E + ++L Sbjct: 192 FPKPENIITKEEAIKIFKEKIGLTLQYALILPQLEKESY--------KTLLVYGVYQNAP 243 Query: 306 IDNEGAEASALPITYEAVPQAK---------------ERFQAAGTGKEITAAEAAKLVQQ 350 ID E P+ Y +P + E+ + + K I+ +A ++ + Sbjct: 244 IDAFTGEVKK-PLYYYPMPGGRTAGPAGEKEMQLTPEEKKEIEASEKYISKDKALEIAKN 302 Query: 351 VVAIPAE-RKPDNQSLGTG--YPNTKQKVWRLSWRDNSNTRMAAFPASSYAEVDAATGQI 407 + E K ++ SL T Y K W W + + AS VDA TG++ Sbjct: 303 SLPFSLEGYKLESASLYTDTYYAPAKNPTWSFYWNLQKDNKYFYVNAS----VDAVTGKL 358 Query: 408 YQYQLEQFTGSKDVKPQPAPAGGKKLTQAEAKQQAILLVNRLYDQASSVLKLAEHGGTWS 467 Y + + +D QP K T E + A + R+ + K Sbjct: 359 -TYFSKTVSDIEDKDKQP------KYTSKELGEIAEKYLERIIPEKFKNTKYFNETSNPD 411 Query: 468 VLPDGKGFRFQFVRYYNGIPVSDTGVTLAMDIY-GRLESY--SAANPDFTTIQGQPTPAI 524 P+ + F ++R NGI +T+ ++ Y G + SY + N +FT + I Sbjct: 412 YSPNL--YTFTYLRTVNGILAPFDNITITLNPYTGEITSYYLNWTNVNFTPAEN----VI 465 Query: 525 SQSEAMQSYKDRYAMKLQYNRIGGYNNIGYVEPQVKLVYSPLPVNEQNQYEVLDAATGKW 584 + EA + D Y MKL Y Y + P+VKLVY + + Y +DA TG Sbjct: 466 TLDEAYKILLDNYEMKLNYILDYDYKSPD-APPKVKLVY------QLDFYGYIDAKTGTL 518 Query: 585 VKVNAYSGQNAGTPAAVDLKGHPAEQALTELVKYGVLVPDADGKINPDQEITVGDWFTYI 644 + D++G+ AE+ + L +YG++ + D K PD+EIT D+ + Sbjct: 519 LDGMGRPIVKESKVTFTDIEGNWAEKDIKLLAEYGIIEVEGD-KYYPDKEITQKDFIKML 577 Query: 645 AAASTSSYQGYTNGTEQKPVAGVNPDSEYYKAVSFAANHEWISKDTVL----QPDNKLSR 700 Y P+ + D E Y ++ ISK + PD+ ++R Sbjct: 578 VKTVQPPYYN--------PMPKSSKDYETYYTIA-------ISKKIIAPEEKNPDSPVTR 622 Query: 701 EQLAVQLASFLQYSKISVFLENDTAVSSFSDSASI-KNK-GAVALAVKLGLMQGDNGKFN 758 + A L + LQ I+ + N ++S F D I KN G VA+ L +M+G +G F Sbjct: 623 LEAAKMLVNALQVGYIAE-IPNIFSIS-FKDKNEIPKNMIGYVAIISGLNIMRGSDGYFY 680 Query: 759 PQQNVTKAGVATVLMKLVEL 778 PQ+ +T+A A VL++ +++ Sbjct: 681 PQRPLTRAEAAAVLVRYLQV 700 >ref|ZP_00061389.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 731 Score = 89.7 bits (221), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 158/711 (22%), Positives = 310/711 (43%), Gaps = 87/711 (12%) Query: 113 IWNISWSYEIGGTSYGFSTQVDALTGDLINTYITYPFLQQDAYYPP-KLTRADALEQARK 171 +WN+ W + G S VD+ TGD++N Y Q D+ +P ++R +A +A + Sbjct: 63 LWNLEWQTK-GDEGSIVSVSVDS-TGDILNYYYYNYMHQYDSKFPKISISRDEAKTKAEE 120 Query: 172 FIAKAAPSLGPDDIQLSDNEAMNPVGGSLFGTV-QYNFYFKLLK--NGLPSASDVLSVTV 228 I K P + +++ + + + ++ +YF+ ++ NG+P S+ +S+ + Sbjct: 121 IIEKLNPGIL---------DSLKFIQANQYTSIYDRAYYFRYIRTYNGIPIPSNDISIAI 171 Query: 229 NG-NGDVLQFSKT-APGPEYPSAKPAISLESAQKKFTDGFAVGLYYIPVYKDNTIEKWVL 286 + G+++ ++KT +PSA+ ISL+ AQ+ + + L Y V K++++ + Sbjct: 172 DKQTGELVSYNKTWNKDVIFPSAEKIISLKEAQEAYIKNLGLRLTYNAVIKNDSVRVFP- 230 Query: 287 GWRPQEQSLYSINAQTGKSIDNEGAEASALPITY-EAVPQAKERFQA-------AGTGKE 338 + P S Y I+A TG+ +GAE IT+ EAV ++ F + A T +E Sbjct: 231 AYTPIYGSGYYIDAFTGERT-MQGAE---FVITFNEAVKKSMSLFDSGMGSQGVALTPEE 286 Query: 339 ITAAE-AAKLVQQVVAIPAERK-----------PDNQSLGTGYPNTKQKVWRLSWRDNSN 386 I A E AA L+ Q A R+ D +L +P W L + N++ Sbjct: 287 IKAVEEAAGLITQEEAEKIARELKVLELDDTFVVDWANLYKEWPTRTNYNWDLRFVKNAD 346 Query: 387 TRMAAFPASSYAEVDAATGQIYQYQLEQFTGSKDVKPQPAPAGGKKLTQAEAKQ-QAILL 445 + +S ++A TG+I + + + VK A KK+ + K+ Q Sbjct: 347 KTGEDY-SSVDVSINAKTGEIVSFNINKANKEDTVKFDREAA--KKIVEKFLKEIQPEKF 403 Query: 446 VNRLYDQAS--SVLKLAEHGGTWSVLPDGKGFRFQFVRYYNGIPVSDTGVTLAMD-IYGR 502 YD+ + +++ AE + + P F + R NG+P G+T+ D + G+ Sbjct: 404 KETEYDETTDRAIILYAEKSYSQNEQP--AYHVFNYTRKVNGVPFPGNGITVGFDAVTGK 461 Query: 503 LESYSAA--NPDFTTIQGQPTPAISQSEAMQSYKDRYAMKLQYNRIGGYNNIGYVEP--- 557 +++Y + +F +++ A+ + + + + ++LQY G NN Y+E Sbjct: 462 IQNYKVEWYDIEFPSVE----KALEIEKIYEIFFEEIGLELQYIIDG--NNAVYIEKVAS 515 Query: 558 ----------QVKLVYSPLPVNEQNQYEVLDAATGKWVKVNAYSGQNAGTPAAVDLKGHP 607 + +LVY+ VN + +LDA TG + + + D+ H Sbjct: 516 DAVASPDNKTEARLVYA---VNTKKP-AILDAYTGTILYGDGTPYKEDKVLEYTDISDHY 571 Query: 608 AEQALTELVKYGVLVPDADGKINPDQEITVGDWFTYIAAASTSSYQGYTNGTEQKPVAGV 667 A++ + + + G+ + + K PD++I D+ I+ Y Y + ++ Sbjct: 572 AKKQIEMMAEMGIGLEGPELK--PDEKIKQKDFLLLISQV-LGEYPFYGKTS----LSSD 624 Query: 668 NPDSEYYKAVSFAANHEWISKDTVLQPDNKLSREQLAVQLASFLQYSKISVFLENDTAVS 727 + E YK + + I K+ P+ L+RE+ + L+Y K++ E Sbjct: 625 DETEELYKTLI----RQGIVKEGEKNPEAYLTREESVKFVIRALKYDKVADISEIFNCTF 680 Query: 728 SFSDSASIKNKGAVALAVKLGLMQGDNGKFNPQQNVTKAGVATVLMKLVEL 778 D + + G V +A L ++ G F P+ + +A ++ +++ Sbjct: 681 KDKDEINPELIGYVVIAKGLKIVNGHGEYFRPKDELKRADALIIIYNYLQI 731 >ref|ZP_00100195.1| COG3468: Type V secretory pathway, adhesin AidA [Desulfitobacterium hafniense] Length = 2207 Score = 61.2 bits (147), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 52/194 (26%), Positives = 93/194 (47%), Gaps = 29/194 (14%) Query: 599 AAVDLKGHPAEQALTELVKYGVLVPDADGKINPDQEITVGDWFTYIAAASTSSYQGYTNG 658 A D+ GH A++ + + G+ ++ K +P+ +T G + T + + + GY Sbjct: 1926 AFTDIAGHWAKEDIEFVASRGLFGGTSETKFSPNTAMTRGMFVTALGRLANADVSGY--- 1982 Query: 659 TEQKPVAGVNPDSEYYKAVSFAANHEWISKDTVLQ--------PDNKLSREQLAVQLASF 710 + + V D+ Y + EW SK+ ++ PD ++REQ+AV ++++ Sbjct: 1983 -AKSSFSDVQDDAYYMGYI------EWASKNNIVNGVGNGKFAPDQSITREQMAVIMSNY 2035 Query: 711 ---LQYSKISVFLENDTAVSSFSDSASIKN--KGAVALAVKLGLMQGDNGK-FNPQQNVT 764 + Y+ V +EN F+D+A I K AV G++ G NG ++PQ T Sbjct: 2036 AKTIGYTLPKVHIEN-----IFTDNAKISTYAKEAVKQMQMAGVISGKNGNLYDPQGTAT 2090 Query: 765 KAGVATVLMKLVEL 778 +A V+ VL + VEL Sbjct: 2091 RAEVSAVLRRFVEL 2104 >emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] emb|CAA61884.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] Length = 1321 Score = 60.1 bits (144), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 50/180 (27%), Positives = 85/180 (47%), Gaps = 17/180 (9%) Query: 602 DLKGHPAEQALTELVKYGVLVPDADGKINPDQEITVGDWFTYIAAASTSSYQGYTNGTEQ 661 D++GH AE+ L + ++ G+LV DG I PD IT ++ T I + Y EQ Sbjct: 32 DIRGHWAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKV----FGLYELSREQ 87 Query: 662 KPVAGVNPDSEYYKAVSFAANHEWIS--KDTVLQPDNKLSREQLAVQLASFLQYSKISVF 719 A V Y + + A +I+ V +PDN ++R++ V +A + S + Sbjct: 88 --FADVEDSKWYSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNY 145 Query: 720 LENDTAVSSFSDSASIKN--KGAVALAVKLGLMQG-DNGKFNPQQNVTKAGVATVLMKLV 776 S F D + +K K +V+ V+ G + G ++G F P +T+A +L K++ Sbjct: 146 ------TSKFKDGSLVKEYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILNKII 199 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.129 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,177,780,688 Number of extensions: 50357113 Number of successful extensions: 87149 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 87174 Number of HSP's successfully gapped: 11 Length of query: 792 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 658 Effective length of database: 393,414,312 Effective search space: 258866617296 Effective search space used: 258866617296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)