BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0149.0038.p (315 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAE51043.1| unknown [environmental sequence] 161 2e-38 ref|NP_069647.1| phosphoglycerate dehydrogenase (serA) [Archaeog... 158 2e-37 ref|NP_276105.1| phosphoglycerate dehydrogenase [Methanothermoba... 153 6e-36 gb|EAJ95830.1| unknown [environmental sequence] 144 4e-33 ref|NP_613584.1| Predicted dehydrogenase related to phosphoglyce... 143 6e-33 >gb|EAE51043.1| unknown [environmental sequence] Length = 271 Score = 161 bits (408), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 96/267 (35%), Positives = 144/267 (53%), Gaps = 10/267 (3%) Query: 47 IPEHIKVDHSLLSIAKKLKLVQT-GAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMA 105 +P++++VD LS L +V + GAG+D +DV ACT G+ EH + Sbjct: 9 VPKYLQVDKQFLSKVPNLIVVSSSGAGYDTIDVEACTDAGVLVVNQTGGNAEGVAEHAVG 68 Query: 106 LILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRV-FDMN 164 +IL+ +K I D ++ +E ++ G +L VA C+V F+ N Sbjct: 69 MILNLFKRIGECDHALRRGWNEPRVSLMGKDLLNKNVGIIGLGNTGGRVAEICKVAFNCN 128 Query: 165 VLAYARNAVVQSDGFVKM----TDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNS 220 +LAY + + + F K T+ D LL+ SD++SVHIPLN++T +INK+ FKKMK Sbjct: 129 ILAY--DPYLSDEAFKKKNANKTELDILLAESDVVSVHIPLNKETNNMINKSWFKKMKKG 186 Query: 221 TLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGM 280 + F+ T+RG I NE DL D L G ++GA LDV+E EP P +L NVI +PHTAG+ Sbjct: 187 SYFVTTSRGSIHNEDDLYDILSEGHLAGAGLDVWEFEPPPSTHKLLKHENVIASPHTAGI 246 Query: 281 PDGRKFHKKRYDFFINNIKRIENGEEP 307 R K +F + I +G++P Sbjct: 247 T--RDSRNKMSEFVATQLLDIFDGKDP 271 >ref|NP_069647.1| phosphoglycerate dehydrogenase (serA) [Archaeoglobus fulgidus DSM 4304] sp|O29445|SERA_ARCFU D-3-phosphoglycerate dehydrogenase (PGDH) pir||E69351 phosphoglycerate dehydrogenase (serA) homolog - Archaeoglobus fulgidus gb|AAB90429.1| phosphoglycerate dehydrogenase (serA) [Archaeoglobus fulgidus DSM 4304] Length = 527 Score = 158 bits (399), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 91/250 (36%), Positives = 136/250 (54%), Gaps = 7/250 (2%) Query: 34 QEMLHHIEDCQ-VIIPEHIKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXX 91 +E++ + + +++ KVD ++ AK LK++ + G G DN+D+ A TQ GI Sbjct: 33 EELIREVPKYEAIVVRSQTKVDAEVIQAAKNLKIIGRAGVGVDNIDINAATQRGIVVVNA 92 Query: 92 XXXXXXXXXEHVMALILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXX 151 EH +AL+L+ + IP D +K E + + G EL Sbjct: 93 PGGNTISTAEHAIALMLAAARKIPQADRSVKEGKWERK-KFMGIELRGKTAGVIGLGRVG 151 Query: 152 XXVAAFCRVFDMNVLAY---ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQL 208 VA C+ +MNVLAY + G VK+ DFDTLL++SD+I+VH+P ++T L Sbjct: 152 FEVAKRCKALEMNVLAYDPFVSKERAEQIG-VKLVDFDTLLASSDVITVHVPRTKETIGL 210 Query: 209 INKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNL 268 I K F+KMK+ + +N ARGGIV+E L +A+K G ++ A LDV+E EP D+ L L Sbjct: 211 IGKGQFEKMKDGVIVVNAARGGIVDEAALYEAIKAGKVAAAALDVYEKEPPSPDNPLLKL 270 Query: 269 GNVILTPHTA 278 NV+ TPH A Sbjct: 271 DNVVTTPHIA 280 >ref|NP_276105.1| phosphoglycerate dehydrogenase [Methanothermobacter thermautotrophicus] sp|O27051|SERA_METTH D-3-phosphoglycerate dehydrogenase (PGDH) pir||H69229 phosphoglycerate dehydrogenase - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB85466.1| phosphoglycerate dehydrogenase [Methanothermobacter thermautotrophicus str. Delta H] Length = 525 Score = 153 bits (386), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 106/321 (33%), Positives = 157/321 (48%), Gaps = 20/321 (6%) Query: 1 MKILIVGYFNETSKSNIARYFPQDWNVVVVPPGQEMLHHIEDC-QVIIPEHIKVDHSLLS 59 MK+LI NE S + N + P +E+L I+D +++ KV ++ Sbjct: 4 MKVLIADSINEKGISELEEVAEVVVNTTITP--EELLDAIKDFDAIVVRSRTKVTREVIE 61 Query: 60 IAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMALILSYYKNIPFLD 118 A +LK++ + G G DNVDV A T GI EH + L+L+ + I D Sbjct: 62 AAPRLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIAD 121 Query: 119 TFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFCRVFDMNVLAY----ARNAVV 174 +K E + G EL V + F M+++ Y ++ A Sbjct: 122 RSVKEGKWEKN-RFMGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAE 180 Query: 175 QSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNSTLFINTARGGIVNE 234 + V +TD +TLL SDI+++H+PL +T+ LI++ FK MK++ +N ARGGI++E Sbjct: 181 EMG--VTVTDLETLLRESDIVTIHVPLTPETRHLISEDEFKLMKDTAFIVNCARGGIIDE 238 Query: 235 RDLIDALKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMPDGRKFHKKRYDFF 294 L ALK+G+I+GA LDVFE EP P S L L NV+LTPH G + + D Sbjct: 239 DALYRALKDGEIAGAALDVFEEEP-PEGSPLLELENVVLTPHI-----GASTSEAQRDAA 292 Query: 295 I---NNIKRIENGEEPESKLN 312 I N IK + G P + LN Sbjct: 293 IIVANEIKTVFQGGAPRNVLN 313 >gb|EAJ95830.1| unknown [environmental sequence] Length = 341 Score = 144 bits (362), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 95/272 (34%), Positives = 135/272 (49%), Gaps = 10/272 (3%) Query: 47 IPEHIKVDHSLLSIAKKLKLVQTG-AGFDNVDVPACTQLGIWXXXXXXXXXXXXXEHVMA 105 +PE +VD LS L LV +G AGFD +DV ACT+ GI EH + Sbjct: 58 VPEIFQVDEEFLSKTPNLLLVSSGGAGFDTIDVEACTKRGILVVNQTGGNAEAVAEHAVG 117 Query: 106 LILSYYKNIPFLDTFMKNKMDESQLDYTGSELXXXXXXXXXXXXXXXXVAAFC-RVFDMN 164 ++++ K I D F++ D + ++ G L VA C + F+ Sbjct: 118 MMINLLKKINESDRFLRQGWDGERTEFLGLNLFNKTIGVIGLGNTGGRVAEICIKGFNCK 177 Query: 165 VLA---YARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQLINKAVFKKMKNST 221 +L+ Y N G K + D LLS SDI++VHIPLN +TK +IN F KMK + Sbjct: 178 ILSFDPYVPNERFNEIG-AKKSKLDYLLSDSDIVTVHIPLNNETKNMINLEFFLKMKKGS 236 Query: 222 LFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRNLGNVILTPHTAGMP 281 F+ T+RG I NE DL + +G +SGA LDV++ EP +L NVI TPH AG+ Sbjct: 237 YFVTTSRGSIHNENDLEKVINSGHLSGAGLDVWDKEPPDASHKLLKYKNVIATPHIAGVT 296 Query: 282 -DGRKFHKKRYDFFINNIKRIENGEEPESKLN 312 D R +K +F + I +G P+ +N Sbjct: 297 IDSR---RKMSEFVAKQLLTIMSGGNPKRPVN 325 >ref|NP_613584.1| Predicted dehydrogenase related to phosphoglycerate dehydrogenase [Methanopyrus kandleri AV19] gb|AAM01514.1| Predicted dehydrogenase related to phosphoglycerate dehydrogenase [Methanopyrus kandleri AV19] Length = 522 Score = 143 bits (360), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 93/285 (32%), Positives = 140/285 (49%), Gaps = 10/285 (3%) Query: 34 QEMLHHIEDCQV-IIPEHIKVDHSLLSIAKKLKLV-QTGAGFDNVDVPACTQLGIWXXXX 91 +E+ H+ D ++ +V L+ AK LK++ + G G DN+DV A T+ GI Sbjct: 33 EEIREHVRDADAWVVRSGTRVTRELIEEAKNLKVIARAGVGVDNIDVKAATERGIIVVNA 92 Query: 92 XXXXXXXXXEHVMALILSYYKNIPFLD-TFMKNKMDESQLDYTGSELXXXXXXXXXXXXX 150 EH M LIL+ + IP D + + + D + + G EL Sbjct: 93 PESSSISVAEHTMGLILALARKIPQADRSVRRGEWDRKR--FMGVELAGKTLGLIGLGRI 150 Query: 151 XXXVAAFCRVFDMNVLAY---ARNAVVQSDGFVKMTDFDTLLSTSDIISVHIPLNQQTKQ 207 VA + F+M V AY V + G + + + LL +D++S+H+PL ++T+ Sbjct: 151 GQQVAKRAKAFEMEVTAYDPYIPEKVAEELGVELVDELEELLERADVVSIHVPLTEETEG 210 Query: 208 LINKAVFKKMKNSTLFINTARGGIVNERDLIDALKNGDISGACLDVFESEPLPIDSELRN 267 +I + K+MK+S +N ARG IV+E LI ALK G I+GA LDVF EP D L Sbjct: 211 MIGEEELKRMKSSAFLVNCARGKIVDEEALIKALKEGWIAGAALDVFAEEPPGEDHPLYE 270 Query: 268 LGNVILTPHTAGMPDGRKFHKKRYDFFINNIKRIENGEEPESKLN 312 L NV+LTPH G + + I+R+ GE PE+ +N Sbjct: 271 LDNVVLTPHIGGSTG--EAQRAAGLIVAREIERVLKGEIPENVVN 313 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 421,178,805 Number of extensions: 15906096 Number of successful extensions: 43241 Number of sequences better than 1.0e-05: 2215 Number of HSP's gapped: 42216 Number of HSP's successfully gapped: 2215 Length of query: 315 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 190 Effective length of database: 417,850,203 Effective search space: 79391538570 Effective search space used: 79391538570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)