BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0024.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] 337 2e-91 emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] 326 4e-88 ref|NP_241311.1| ABC transporter (ATP-binding protein) [Bacillus... 324 1e-87 pir||JC5348 cdd4 protein - Clostridium difficile >gi|1770140|emb... 300 4e-80 ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system,... 286 4e-76 >emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] Length = 311 Score = 337 bits (864), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 175/310 (56%), Positives = 234/310 (75%), Gaps = 5/310 (1%) Query: 1 MLVTEVKKMTETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLL 60 M+V EV+++ E +++T++L K + AV +NL I KGEIYGFLGPNGAGKTTTIRMLL Sbjct: 1 MIVPEVRRL-EYIVQTQNLSKNFGKEQAVSNINLQIRKGEIYGFLGPNGAGKTTTIRMLL 59 Query: 61 GLIKPTSGSINIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSR 120 GL+KP+SG+I IF +D+ ++++QIL VGSLVE+PSYY HL+A +NLEA+R+IL VPK+R Sbjct: 60 GLMKPSSGTIKIFQKDIVKDRIQILANVGSLVENPSYYPHLTAYENLEALRKILGVPKTR 119 Query: 121 IAEVLEIVSLTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIR 180 I EVL+ V L + VKGFSLGMKQRLGIAAALL +PELLILDEPTNGLDPSGI EIR Sbjct: 120 IDEVLQTVRLKEAANKKVKGFSLGMKQRLGIAAALLHNPELLILDEPTNGLDPSGIIEIR 179 Query: 181 SLIKRLPVEEGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAV 240 +LIKRLP E G+T+++SSHLLSEI+QMA VGI+ G +++QD+I +++ A + V Sbjct: 180 NLIKRLPKEYGMTIIISSHLLSEIDQMATQVGIVSKGTLIFQDSIEGMRKFAQPKVTFKV 239 Query: 241 SEPEEAL--ILANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSL 298 S E+ +LAN G Q+ I L SD +VA +++ LV+ G +++RVEE ++SL Sbjct: 240 SNGEKGWRSLLAN--GIKANYQDDVIFLEECSDEKVAHIVQVLVQEGISVFRVEEEKRSL 297 Query: 299 EEFFLQVVEG 308 E+ FLQ+ G Sbjct: 298 EDIFLQMTAG 307 >emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] Length = 303 Score = 326 bits (836), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 166/298 (55%), Positives = 224/298 (75%), Gaps = 1/298 (0%) Query: 9 MTETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSG 68 M + ++ T DL K+Y+ +V+ LNL I +GEIYGFLGPNGAGKTTTIRMLLGLIKPT G Sbjct: 1 MNDQIVVTHDLTKKYKKHTSVDGLNLRIRRGEIYGFLGPNGAGKTTTIRMLLGLIKPTKG 60 Query: 69 SINIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSRIAEVLEIV 128 +I IFGQ+L +N+LQIL+++GSLVESP+YYG+L+ +NLEA+RR+ +P+ ++ EVLE V Sbjct: 61 NIEIFGQNLNKNRLQILQRIGSLVESPTYYGNLTGYENLEAVRRLRGLPEQQVNEVLETV 120 Query: 129 SLTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPV 188 L+ R K +SLGMKQRLGIA ALL SP+LLILDEPTNGLDPSGIQEIR LIK LP Sbjct: 121 RLSKVANRLTKEYSLGMKQRLGIAVALLSSPDLLILDEPTNGLDPSGIQEIRELIKELP- 179 Query: 189 EEGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALI 248 + G++V+VSSHLLSEI+QMA VGII G+M++QD+IA L Q+ L++ VS+ EA Sbjct: 180 KSGMSVIVSSHLLSEIDQMATQVGIINNGKMIFQDSIASLHQKRKPLLKVGVSDVIEAKT 239 Query: 249 LANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQVV 306 + NR+G LQ+ + L + V+ + L+ +G +++R+EE +SLE+ FL++ Sbjct: 240 ILNRKGLKVDLQKNYLWLSQTEPEFVSEINSILLHSGLSVFRLEEKTRSLEDIFLELT 297 >ref|NP_241311.1| ABC transporter (ATP-binding protein) [Bacillus halodurans] pir||E83705 ABC transporter (ATP-binding protein) BH0445 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04164.1| ABC transporter (ATP-binding protein) [Bacillus halodurans] Length = 306 Score = 324 bits (831), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 172/303 (56%), Positives = 224/303 (73%) Query: 10 TETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGS 69 T +IET +L Y+ AV+++NL + +GEIYGFLGPNGAGKTTTIRMLLGLIK + GS Sbjct: 4 TNYLIETNNLTMTYKHVHAVDRINLKVKEGEIYGFLGPNGAGKTTTIRMLLGLIKASHGS 63 Query: 70 INIFGQDLKRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSRIAEVLEIVS 129 I IFG+DLK+++L IL+ +G+LVESPSYY HLS DNLE +R+I+ VPKSRIAEVLE+V Sbjct: 64 IKIFGKDLKQHRLDILKNIGALVESPSYYPHLSGEDNLETVRKIVKVPKSRIAEVLELVR 123 Query: 130 LTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVE 189 LT R V+ +SLGMKQRL IAAALL +P L+ILDEPTNGLDP+GI EIR+LI RLP E Sbjct: 124 LTKVADRKVQEYSLGMKQRLAIAAALLANPRLVILDEPTNGLDPAGIIEIRNLILRLPKE 183 Query: 190 EGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALIL 249 GITVL+SSHLLSE+EQ+A VGII G++++QD+I +L+++A + L+ S PE A + Sbjct: 184 YGITVLLSSHLLSEVEQVASQVGIIAKGKLIFQDSIEELRKKANRKIFLSTSHPEVAATI 243 Query: 250 ANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQVVEGG 309 RG Q + + SD +A L+K VE +YR+ + SLEE FLQ+ +GG Sbjct: 244 LQERGINVDTQARGLVMNDRSDEEIAQLVKAFVEKDIDVYRISDETSSLEEIFLQLTKGG 303 Query: 310 QSV 312 Q++ Sbjct: 304 QTL 306 >pir||JC5348 cdd4 protein - Clostridium difficile emb|CAA63569.1| cdd4 [Clostridium difficile] Length = 301 Score = 300 bits (767), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 145/297 (48%), Positives = 215/297 (72%), Gaps = 1/297 (0%) Query: 11 ETVIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGSI 70 E +I T+ L KEY V+ ++L + +G +YGFLGPNGAGK+TT++MLLGL KPT G+I Sbjct: 2 EMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKSTTLKMLLGLAKPTQGNI 61 Query: 71 NIFGQDL-KRNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSRIAEVLEIVS 129 NI G++L ++N++ IL+ +GSL+ESPSYYGHL+ L+N+ ++R+LD+PK + E L+IV Sbjct: 62 NILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIMQRLLDLPKKNVNEALKIVR 121 Query: 130 LTGEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVE 189 L ++ + V +SLGMKQRLGIA A++ P+LLILDEPTNGLDP+GI+EIR LIK LP + Sbjct: 122 LENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPTNGLDPAGIEEIRELIKSLPEK 181 Query: 190 EGITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALIL 249 G+TVL+SSHLLSEI+Q+A ++GII G +V+QD+I +L + + + A L Sbjct: 182 YGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIKELHNRGQSQIAIKTKNVVSAQKL 241 Query: 250 ANRRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQVV 306 N +G +Q+G +T+ + D VA + LV + ++R+EEH++SLE+ FL+++ Sbjct: 242 LNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHSNIDVFRIEEHKKSLEDIFLELL 298 >ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system, ATPase component [Desulfitobacterium hafniense] Length = 304 Score = 286 bits (732), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 143/295 (48%), Positives = 202/295 (68%), Gaps = 1/295 (0%) Query: 13 VIETRDLLKEYRGRAAVEQLNLNIGKGEIYGFLGPNGAGKTTTIRMLLGLIKPTSGSINI 72 +I+T+ L K Y V L+L++ +G +YGFLGPNGAGK+TT++M+LGL+KPT+GSI + Sbjct: 4 IIKTQGLCKHYGKTPGVSDLDLSVAEGAVYGFLGPNGAGKSTTLKMILGLVKPTAGSITV 63 Query: 73 FGQDLK-RNKLQILRKVGSLVESPSYYGHLSALDNLEAIRRILDVPKSRIAEVLEIVSLT 131 FG+ + R+++ IL+ VGSL+ESPSYYGHLS +NL I + P+ I VLEIV L Sbjct: 64 FGKTMNHRSRMSILKDVGSLIESPSYYGHLSGYENLRIIATLKGTPEKDIGRVLEIVRLE 123 Query: 132 GEEKRPVKGFSLGMKQRLGIAAALLGSPELLILDEPTNGLDPSGIQEIRSLIKRLPVEEG 191 ++ + +SLGMKQRLG+A ALLG+P LLILDEPTNGLDP+GIQE+R LI+ LP E G Sbjct: 124 NQKNKKTNQYSLGMKQRLGLACALLGNPRLLILDEPTNGLDPAGIQEMRELIRSLPKEYG 183 Query: 192 ITVLVSSHLLSEIEQMAGTVGIIRAGRMVYQDTIAQLQQQAAGDLRLAVSEPEEALILAN 251 ITVLVSSHLLSEIEQMA +GII GR+V+Q ++ L Q+++ + + + A + Sbjct: 184 ITVLVSSHLLSEIEQMATNLGIISKGRLVFQSSLGALHQKSSPSIAIRTLDNHRAAAVLR 243 Query: 252 RRGCGGTLQEGRITLPRMSDVRVALLIKELVENGHAIYRVEEHRQSLEEFFLQVV 306 +G Q+ + LP +D ++ L L E + R+E+ +SLE+ FL++ Sbjct: 244 EQGIAVQCQQDYLLLPAFADGELSRLTLNLFEQKVGVVRIEQREKSLEDIFLELT 298 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 431,532,188 Number of extensions: 17767540 Number of successful extensions: 120710 Number of sequences better than 1.0e-05: 20079 Number of HSP's gapped: 101203 Number of HSP's successfully gapped: 21423 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)