BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0147.0011.p (373 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098913.1| COG1613: ABC-type sulfate transport system, p... 436 e-121 ref|ZP_00023867.1| COG1613: ABC-type sulfate transport system, p... 432 e-120 ref|NP_977518.1| sulfate ABC transporter, sulfate/thiosulfate-bi... 429 e-119 ref|NP_756723.1| Sulfate-binding protein precursor [Escherichia ... 424 e-117 ref|ZP_00060257.1| COG1613: ABC-type sulfate transport system, p... 423 e-117 >ref|ZP_00098913.1| COG1613: ABC-type sulfate transport system, periplasmic component [Desulfitobacterium hafniense] Length = 377 Score = 436 bits (1122), Expect = e-121, Method: Compositional matrix adjust. Identities = 205/310 (66%), Positives = 251/310 (80%), Gaps = 1/310 (0%) Query: 60 KDPVELLNVSYDPTRELYENYNKAFSAYWEKETGQKVTVKQSHGGSGAQSRAVLEGLEAD 119 K PV LLNVSYDPTRELY+ YN AFS YW+++TGQ VT++QSHGGSG+Q+R V++G EAD Sbjct: 65 KPPVSLLNVSYDPTRELYQEYNIAFSKYWKEKTGQDVTIQQSHGGSGSQARTVIDGNEAD 124 Query: 120 VVTLALGYDIDALKD-KGLINEGWESKFEHNSSPYTSTIVFLVRKGNPKGIKDWPDLLKE 178 VVTLAL YDID++ K L+N W+ + +NS+PYTSTIVFLV+KGNPK IKDW DL++ Sbjct: 125 VVTLALAYDIDSINSQKSLLNPEWQKRLPNNSTPYTSTIVFLVKKGNPKNIKDWNDLVQP 184 Query: 179 GVEVITPNPKTSGGARWNYLAAWGYALDHNNNDEAKAQEFVQELFKHVPVLDTGARGATT 238 GV VITPNPKTSGGARWNYLAAW YAL+HNNND KA+EFV+ L+K+VPVLD+GARG+TT Sbjct: 185 GVSVITPNPKTSGGARWNYLAAWAYALEHNNNDAQKAKEFVEALYKNVPVLDSGARGSTT 244 Query: 239 TFVERGIGDVLIAWENEAYLSIKELGPDKFEIVNPSESILXXXXXXXXXXXXXXRGTREA 298 TFVERGIGDVL+AWENEA+LS++E G DKFEIV PS SIL +GTRE Sbjct: 245 TFVERGIGDVLLAWENEAFLSLREFGEDKFEIVVPSLSILAEPPVTVVDSVVDKKGTREV 304 Query: 299 AEAYLKYLYTEEGQKIAADNYYRPTLESVKEEYKDKFPEIKLFTLADKFGTWKETQEKHF 358 AEAYL YLY++EGQ+IAA N+YRP E+V ++Y +FP+I LFT+ D FG W E Q++HF Sbjct: 305 AEAYLDYLYSDEGQEIAAKNFYRPRNEAVAQKYAAQFPQINLFTIDDVFGGWAEAQQEHF 364 Query: 359 NDGGIFDKIY 368 DGG+FD+IY Sbjct: 365 ADGGVFDQIY 374 >ref|ZP_00023867.1| COG1613: ABC-type sulfate transport system, periplasmic component [Ralstonia metallidurans] Length = 362 Score = 432 bits (1112), Expect = e-120, Method: Compositional matrix adjust. Identities = 205/309 (66%), Positives = 246/309 (79%) Query: 65 LLNVSYDPTRELYENYNKAFSAYWEKETGQKVTVKQSHGGSGAQSRAVLEGLEADVVTLA 124 LLNVSYDPTRELY + N AF+ ++ ++G VT++QSHGGSG Q+R+V++GL+ADVVTLA Sbjct: 54 LLNVSYDPTRELYVDVNAAFAKAYKAQSGDAVTIRQSHGGSGKQARSVIDGLDADVVTLA 113 Query: 125 LGYDIDALKDKGLINEGWESKFEHNSSPYTSTIVFLVRKGNPKGIKDWPDLLKEGVEVIT 184 LGYDIDA+ DKGLI W+ + HN+SPYTSTIVFLVRKGNPK IKDW DL+K GV+VIT Sbjct: 114 LGYDIDAIADKGLIKPDWQKRLPHNASPYTSTIVFLVRKGNPKQIKDWNDLIKPGVQVIT 173 Query: 185 PNPKTSGGARWNYLAAWGYALDHNNNDEAKAQEFVQELFKHVPVLDTGARGATTTFVERG 244 PNPKTSGGARWNYLAAWGYAL +EAKA+EFV L K+VPVLD+GARGATTTFVERG Sbjct: 174 PNPKTSGGARWNYLAAWGYALRQPGGNEAKAKEFVGALLKNVPVLDSGARGATTTFVERG 233 Query: 245 IGDVLIAWENEAYLSIKELGPDKFEIVNPSESILXXXXXXXXXXXXXXRGTREAAEAYLK 304 +GDVLIAWENEA L+IKELGPDKF+IV PS SIL +GTR+AAEAYL+ Sbjct: 234 LGDVLIAWENEAILAIKELGPDKFDIVAPSVSILAEPPVAVVDKVVDKKGTRKAAEAYLQ 293 Query: 305 YLYTEEGQKIAADNYYRPTLESVKEEYKDKFPEIKLFTLADKFGTWKETQEKHFNDGGIF 364 +LYTEEGQ+IAA NYYRP E V +Y FP++KLFT+ + FG W + Q+ HF DGG F Sbjct: 294 FLYTEEGQEIAAKNYYRPISEKVAAKYAANFPKVKLFTIDEVFGGWAKAQKAHFADGGSF 353 Query: 365 DKIYVPGAK 373 D++Y PG + Sbjct: 354 DQVYQPGKR 362 >ref|NP_977518.1| sulfate ABC transporter, sulfate/thiosulfate-binding protein [Bacillus cereus ATCC 10987] gb|AAS40126.1| sulfate ABC transporter, sulfate/thiosulfate-binding protein [Bacillus cereus ATCC 10987] Length = 351 Score = 429 bits (1104), Expect = e-119, Method: Compositional matrix adjust. Identities = 206/309 (66%), Positives = 249/309 (80%) Query: 60 KDPVELLNVSYDPTRELYENYNKAFSAYWEKETGQKVTVKQSHGGSGAQSRAVLEGLEAD 119 K PVELLNVSYDPTRELY+++NK F+ YW+++ GQ V VKQSHGGSG+Q+R+V++GLEAD Sbjct: 41 KKPVELLNVSYDPTRELYQDFNKDFAKYWKEKHGQTVNVKQSHGGSGSQARSVIDGLEAD 100 Query: 120 VVTLALGYDIDALKDKGLINEGWESKFEHNSSPYTSTIVFLVRKGNPKGIKDWPDLLKEG 179 VVTLAL YDIDA+ K + E W+ + NS+PYTSTIVFLVRKGNPKGIKDW DL K+G Sbjct: 101 VVTLALAYDIDAISKKKQLAEDWQKRLADNSTPYTSTIVFLVRKGNPKGIKDWDDLTKKG 160 Query: 180 VEVITPNPKTSGGARWNYLAAWGYALDHNNNDEAKAQEFVQELFKHVPVLDTGARGATTT 239 V VITPNPKTSGGARWNYL AWGYAL+ NN E KA+EFV +++K+V VLD+GARGATTT Sbjct: 161 VSVITPNPKTSGGARWNYLGAWGYALEKYNNSEDKAKEFVGQIYKNVEVLDSGARGATTT 220 Query: 240 FVERGIGDVLIAWENEAYLSIKELGPDKFEIVNPSESILXXXXXXXXXXXXXXRGTREAA 299 FVE+GIGDVLIAWENEA LS KELG DKFEIV P SIL +GTR+ A Sbjct: 221 FVEKGIGDVLIAWENEALLSQKELGKDKFEIVTPPISILAEPPVAVVDKVVDKKGTRKVA 280 Query: 300 EAYLKYLYTEEGQKIAADNYYRPTLESVKEEYKDKFPEIKLFTLADKFGTWKETQEKHFN 359 E YL+YLY+E+GQ+IAA N+YRP E V E+Y +FP+++LFT+ + FG WK+ QEKHFN Sbjct: 281 EGYLEYLYSEKGQEIAAKNFYRPRNEKVAEKYASQFPKVQLFTVDELFGGWKKAQEKHFN 340 Query: 360 DGGIFDKIY 368 DGG+FD+IY Sbjct: 341 DGGVFDQIY 349 >ref|NP_756723.1| Sulfate-binding protein precursor [Escherichia coli CFT073] gb|AAN83297.1| Sulfate-binding protein precursor [Escherichia coli CFT073] Length = 329 Score = 424 bits (1089), Expect = e-117, Method: Compositional matrix adjust. Identities = 198/305 (64%), Positives = 246/305 (80%) Query: 63 VELLNVSYDPTRELYENYNKAFSAYWEKETGQKVTVKQSHGGSGAQSRAVLEGLEADVVT 122 ++LLNVSYDPTRELYE YNKAFSA+W+++TG V ++QSHGGSG Q+ +V+ G+EADVVT Sbjct: 22 IQLLNVSYDPTRELYEQYNKAFSAHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVT 81 Query: 123 LALGYDIDALKDKGLINEGWESKFEHNSSPYTSTIVFLVRKGNPKGIKDWPDLLKEGVEV 182 LAL YD+DA+ ++G I++ W + NS+PYTSTIVFLVRKGNPK I DW DL+K GV V Sbjct: 82 LALAYDVDAIAERGRIDKEWIKRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSV 141 Query: 183 ITPNPKTSGGARWNYLAAWGYALDHNNNDEAKAQEFVQELFKHVPVLDTGARGATTTFVE 242 ITPNPK+SGGARWNYLAAWGYAL HNNND+AKAQ+FV+ L+K+V VLD+GARG+T TFVE Sbjct: 142 ITPNPKSSGGARWNYLAAWGYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVE 201 Query: 243 RGIGDVLIAWENEAYLSIKELGPDKFEIVNPSESILXXXXXXXXXXXXXXRGTREAAEAY 302 RGIGDVLIAWENEA L+ ELG DKFEIV PSESIL +GT+E AEAY Sbjct: 202 RGIGDVLIAWENEALLAANELGKDKFEIVTPSESILAEPTVSVVNKVVEKKGTKEVAEAY 261 Query: 303 LKYLYTEEGQKIAADNYYRPTLESVKEEYKDKFPEIKLFTLADKFGTWKETQEKHFNDGG 362 LKYLY+ EGQ+IAA NYYRP V ++Y++ FP++KLFT+ ++FG W + Q++HF +GG Sbjct: 262 LKYLYSPEGQEIAAKNYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGG 321 Query: 363 IFDKI 367 FD+I Sbjct: 322 TFDQI 326 >ref|ZP_00060257.1| COG1613: ABC-type sulfate transport system, periplasmic component [Clostridium thermocellum ATCC 27405] Length = 648 Score = 423 bits (1088), Expect = e-117, Method: Compositional matrix adjust. Identities = 198/309 (64%), Positives = 254/309 (82%), Gaps = 1/309 (0%) Query: 61 DPVELLNVSYDPTRELYENYNKAFSAYWEKETGQKVTVKQSHGGSGAQSRAVLEGLEADV 120 +PV +LNVSYDPTRELY++YN+AF YW+++TG+ + ++QSHGGSG+Q+RAV++GLEADV Sbjct: 337 EPVTILNVSYDPTRELYQDYNEAFRKYWKEKTGKDIVIQQSHGGSGSQARAVIDGLEADV 396 Query: 121 VTLALGYDIDAL-KDKGLINEGWESKFEHNSSPYTSTIVFLVRKGNPKGIKDWPDLLKEG 179 VTLAL YDID++ K+K ++++ W+ + +NS+PYTSTIVFLVRKGNPK IKDW DL + G Sbjct: 397 VTLALAYDIDSINKNKEILSKDWQKRLPYNSTPYTSTIVFLVRKGNPKNIKDWDDLARPG 456 Query: 180 VEVITPNPKTSGGARWNYLAAWGYALDHNNNDEAKAQEFVQELFKHVPVLDTGARGATTT 239 VEVITPNPKTSGGARWNYLAAWGYAL ND KA+EFV+ ++ +VPVLD+GARG+TTT Sbjct: 457 VEVITPNPKTSGGARWNYLAAWGYALKKYGNDPEKAKEFVKAIYANVPVLDSGARGSTTT 516 Query: 240 FVERGIGDVLIAWENEAYLSIKELGPDKFEIVNPSESILXXXXXXXXXXXXXXRGTREAA 299 FVERG+GDVLIAWENEA+LS+ ELG DKFEIV PS SIL +GTRE A Sbjct: 517 FVERGLGDVLIAWENEAFLSLNELGKDKFEIVVPSVSILAEPPVAVVDSVVDKKGTREVA 576 Query: 300 EAYLKYLYTEEGQKIAADNYYRPTLESVKEEYKDKFPEIKLFTLADKFGTWKETQEKHFN 359 EAYL+YLY++EGQ+IAA NYYRP E +K++Y +F E++LFT+ + FG W + Q++HF+ Sbjct: 577 EAYLEYLYSDEGQEIAAKNYYRPRKEEIKQKYASQFAEVELFTIDEVFGGWDKAQKEHFD 636 Query: 360 DGGIFDKIY 368 DGGIFD+IY Sbjct: 637 DGGIFDQIY 645 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 501,700,422 Number of extensions: 20344758 Number of successful extensions: 40474 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 40262 Number of HSP's successfully gapped: 140 Length of query: 373 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 246 Effective length of database: 412,420,005 Effective search space: 101455321230 Effective search space used: 101455321230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 127 (53.5 bits)