BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0003.p (301 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_657543.1| DUF161, Uncharacterized BCR, YitT family COG128... 191 2e-47 ref|NP_388993.1| alternate gene name: yuxA~similar to hypothetic... 189 5e-47 pir||A49921 conserved hypothetical protein yitT - Bacillus subti... 185 1e-45 ref|NP_833368.1| hypothetical Membrane Spanning Protein [Bacillu... 183 4e-45 ref|NP_834689.1| hypothetical Membrane Spanning Protein [Bacillu... 175 1e-42 >ref|NP_657543.1| DUF161, Uncharacterized BCR, YitT family COG1284 [Bacillus anthracis A2012] ref|NP_845963.1| membrane protein, putative [Bacillus anthracis str. Ames] ref|NP_979965.1| membrane protein, putative [Bacillus cereus ATCC 10987] gb|AAP27449.1| membrane protein, putative [Bacillus anthracis str. Ames] gb|AAS42573.1| membrane protein, putative [Bacillus cereus ATCC 10987] Length = 269 Score = 191 bits (485), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 101/251 (40%), Positives = 153/251 (60%), Gaps = 2/251 (0%) Query: 50 RLFLIPHQXXXXXXXXXXXXIGYLTNPKYISLYYFAINLPILIWGFVAVGKKYIFYSLLS 109 LFLIPH+ +G +T P + F +NLPILI G++ +GKK IF +++S Sbjct: 19 NLFLIPHKILSSGIGGIAIILGIVT-PVNTGIINFVLNLPILILGYIGLGKKVIFNTIVS 77 Query: 110 VVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXXXXLRAGGSSGGFDILGSIITRKRD 169 V+ + L +P+ + DP+L GS+GGFDI+G +++RKRD Sbjct: 78 VIVLSVALYYVPVKVVATDPLLSSIFGGVIAGAGIGLVFNCNGSTGGFDIIGMLLSRKRD 137 Query: 170 IPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFVKSRVVDMIHIRHIKLTCFIITKER 229 I +G L +L+ +V++ GFF +WD AL ++L I+V +V+D IH +H K+T I+T E Sbjct: 138 IKLGGFLIILNAVVVIIAGFFFTWDVALTSLLSIYVTGKVIDAIHTKHRKVTLMIVTNEA 197 Query: 230 EKMLNR-LTCLPHGITVVNAEGGYSHEGNTMLMTVTTRYELAELRKTILETDPKSFVNVL 288 EKM + L+ + GIT+++ EG YS E +LMTV +R ELA ++ TI E DP +FVN+ Sbjct: 198 EKMKKQLLSTVVRGITLLDGEGAYSSEKKRVLMTVVSREELASMKLTISEIDPHAFVNIT 257 Query: 289 ETVEIVGRFRR 299 ETVE++G FR+ Sbjct: 258 ETVEVLGLFRK 268 >ref|NP_388993.1| alternate gene name: yuxA~similar to hypothetical proteins [Bacillus subtilis] sp|P39803|YITT_BACSU Hypothetical protein yitT emb|CAB01834.1| putative ORF [Bacillus subtilis] emb|CAA70630.1| YitT [Bacillus subtilis] emb|CAB12952.1| yitT [Bacillus subtilis subsp. subtilis str. 168] Length = 280 Score = 189 bits (481), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 101/278 (36%), Positives = 162/278 (58%), Gaps = 6/278 (2%) Query: 28 VAKQVKEVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTN---PKYISL--Y 82 V Q K++ I+I A L A+GL LFLIP + + + P YIS Sbjct: 2 VLDQTKKLLIVIIGALLNAAGLNLFLIPADVYASGFTGVAQLLSSVVDQYAPFYISTGTL 61 Query: 83 YFAINLPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXX 142 F +N+P+ I G++ VGK + YS+LSV TT F+ ++P L+ D +L Sbjct: 62 LFLLNIPVGILGWLKVGKSFTVYSILSVALTTLFMGILPETSLSHDILLNAVFGGVISAV 121 Query: 143 XXXXXLRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLC 202 L+ G S+GG DI+ ++ + +D P+GT F+L+G++IL+ G + W+ ALY ++ Sbjct: 122 GIGLTLKYGASTGGLDIVAMVLAKWKDKPVGTYFFILNGIIILTAGLLQGWEKALYTLVT 181 Query: 203 IFVKSRVVDMIHIRHIKLTCFIITKEREKMLNRLTC-LPHGITVVNAEGGYSHEGNTMLM 261 ++V +RV+D IH RH+KLT I+TK+ +++ + + GIT V A+G +++E M++ Sbjct: 182 LYVTTRVIDAIHTRHMKLTAMIVTKKADEIKEAIYGKMVRGITTVPAKGAFTNEQKEMMI 241 Query: 262 TVTTRYELAELRKTILETDPKSFVNVLETVEIVGRFRR 299 V TRYEL +L K + E DPK+F N+++T I G FR+ Sbjct: 242 IVITRYELYDLEKIVKEVDPKAFTNIVQTTGIFGFFRK 279 >pir||A49921 conserved hypothetical protein yitT - Bacillus subtilis dbj|BAA03818.1| unnamed protein product [Bacillus subtilis] Length = 280 Score = 185 bits (469), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 99/278 (35%), Positives = 161/278 (57%), Gaps = 6/278 (2%) Query: 28 VAKQVKEVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTN---PKYISL--Y 82 V Q K++ I+I A L A+GL LFLIP + + + P YIS Sbjct: 2 VLDQTKKLLIVIIGALLNAAGLNLFLIPADVYASGFTGVAQLLSSVVDQYAPFYISTGTL 61 Query: 83 YFAINLPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXX 142 F +N+P+ I G++ VGK + YS+LSV TT F+ ++P L+ D +L Sbjct: 62 LFLLNIPVGILGWLKVGKSFTVYSILSVALTTLFMGILPETSLSHDILLNAVFGGVISAD 121 Query: 143 XXXXXLRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLC 202 L+ G S+GG DI+ ++ + +D P+GT F+L+G++IL+ G + W+ ALY ++ Sbjct: 122 GIGLTLKYGASTGGLDIVAMVLAKWKDKPVGTYFFILNGIIILTAGLLQGWEKALYTLVT 181 Query: 203 IFVKSRVVDMIHIRHIKLTCFIITKEREKMLNRLTC-LPHGITVVNAEGGYSHEGNTMLM 261 ++V +RV+D IH RH+KLT I+TK+ +++ + + GIT + A+G +++E M++ Sbjct: 182 LYVTTRVIDAIHTRHMKLTAMIVTKKADEIKEAIYGKMVRGITTLPAKGAFTNEQKEMMI 241 Query: 262 TVTTRYELAELRKTILETDPKSFVNVLETVEIVGRFRR 299 V TRYEL +L K + E DPK+F N+++T I G F + Sbjct: 242 IVITRYELYDLEKIVKEVDPKAFTNIVQTTGIFGFFTK 279 >ref|NP_833368.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] gb|AAP10569.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] Length = 269 Score = 183 bits (465), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 101/263 (38%), Positives = 157/263 (59%), Gaps = 2/263 (0%) Query: 38 IIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTNPKYISLYYFAINLPILIWGFVA 97 +I + ++A LFLIPH+ +G +T P + F +NLPILI G++ Sbjct: 7 VIIGSIIIAVAFNLFLIPHKILSSGIGGIAIILGIVT-PVNTGIINFVLNLPILILGYIG 65 Query: 98 VGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXXXXXXXXXXXXXLRAGGSSGGF 157 +GKK IF +++SVV + L +P+ + DP+L GS+GGF Sbjct: 66 LGKKVIFNTVVSVVVLSVALYYVPVKVVATDPLLSSIFGGVIAGAGIGLVFNCNGSTGGF 125 Query: 158 DILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSALYAMLCIFVKSRVVDMIHIRH 217 DI+G +++RKRDI +G L +L+ +V++ GFF +WD AL ++L I+V +V+D +H +H Sbjct: 126 DIIGMLLSRKRDIKLGGFLIILNAVVVIIAGFFFTWDVALTSLLSIYVTGKVIDAVHTKH 185 Query: 218 IKLTCFIITKEREKMLNR-LTCLPHGITVVNAEGGYSHEGNTMLMTVTTRYELAELRKTI 276 K+T I+T E EKM + L+ + GIT+++ E YS E +LMTV +R EL+ ++ TI Sbjct: 186 RKVTLMIVTNEAEKMKKQLLSTVVRGITLLDGESAYSSEKKRVLMTVVSREELSSMKLTI 245 Query: 277 LETDPKSFVNVLETVEIVGRFRR 299 E DP +FVN+ ETVE++ FRR Sbjct: 246 SEIDPNAFVNITETVEVLRLFRR 268 >ref|NP_834689.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] gb|AAP11890.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] Length = 297 Score = 175 bits (443), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 98/287 (34%), Positives = 161/287 (56%), Gaps = 11/287 (3%) Query: 22 PLVSGPVAKQVK-----EVAIIIFSAFLVASGLRLFLIPHQXXXXXXXXXXXXIGYLTNP 76 +S P AK ++ +I ++ L A + +F IPH IGYL + Sbjct: 14 AFLSFPAAKTFHLEFMIQLVVIFIASILYAISMNMFFIPHNMISGGFAGVGMIIGYLMHY 73 Query: 77 KYISLYYFAINLPILIWGFVAVGKKYIFYSLLSVVATTWFLTVIPLIKLTKDPILXXXXX 136 I F +N+P+LI +GKK F + V ++ + +IP+ +++ D +L Sbjct: 74 N-IGALIFLLNIPLLILSHFYLGKKTTFLTAYFVAVSSLAMNIIPVHQVSDDILLSSVFG 132 Query: 137 XXXXXXXXXXXLRAGGSSGGFDILGSIITRKRDIPMGTVLFVLDGLVILSLGFFKSWDSA 196 R S+GGFD++G I+ + RD+ +G ++FV + +++++ GF WD Sbjct: 133 GVICGAASGIIFRFASSTGGFDVVGLIVAKYRDVSIGAIIFVFNLILLVAAGFIFGWDIT 192 Query: 197 LYAMLCIFVKSRVVDMIHIRHIKLTCFIITKEREKMLNRLTCLPHGI---TVVNAEGGYS 253 LY ++ FV S+V+D +H +HIKLT IT++ E++ + L L HGI T+V+A GGY+ Sbjct: 193 LYTLISRFVVSKVIDAVHTKHIKLTIMTITEKGEEIKSAL--LHHGIRGVTMVDAVGGYT 250 Query: 254 HEGNTMLMTVTTRYELAELRKTILETDPKSFVNVLETVEIVGRFRRL 300 + M+ TV TRYEL E+++ I + D K+F+N+ ETVEIVGRF+R+ Sbjct: 251 NHKKKMIYTVVTRYELGEMKRIIRQVDNKAFMNITETVEIVGRFKRI 297 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.143 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 376,800,886 Number of extensions: 13378563 Number of successful extensions: 40322 Number of sequences better than 1.0e-05: 137 Number of HSP's gapped: 40057 Number of HSP's successfully gapped: 137 Length of query: 301 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 176 Effective length of database: 417,850,203 Effective search space: 73541635728 Effective search space used: 73541635728 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 125 (52.8 bits)