BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0135.0002.p (207 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_693328.1| alkaline phosphatase [Oceanobacillus iheyensis ... 239 2e-62 sp|Q48630|APL_LACLC Alkaline phosphatase like protein >gi|541355... 211 9e-54 ref|NP_266869.1| alkaline phosphatase [Lactococcus lactis subsp.... 211 1e-53 ref|NP_471318.1| similar to alkaline phosphatase [Listeria innoc... 204 9e-52 ref|NP_465395.1| similar to alkaline phosphatase [Listeria monoc... 203 2e-51 >ref|NP_693328.1| alkaline phosphatase [Oceanobacillus iheyensis HTE831] dbj|BAC14363.1| alkaline phosphatase [Oceanobacillus iheyensis HTE831] Length = 202 Score = 239 bits (611), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 106/177 (59%), Positives = 143/177 (80%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M W+T IMEQ GY GIFLM+ALEN+FPPIPSE++LPFGGF+TT + LTI GV+ A+T G Sbjct: 1 MNDWVTGIMEQLGYLGIFLMMALENLFPPIPSEIVLPFGGFLTTYTNLTILGVITAATLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++G+ +LY IG L++ R+ER++DRYG+WIR+K SD+++A+ WF + GYWTV FCRMVP Sbjct: 61 SVIGSMVLYCIGTFLDIPRLERLIDRYGQWIRLKLSDVQRANDWFQRRGYWTVFFCRMVP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYS 177 ++RSLISIPAGM+ M F +F+ TT+GT+ WN +LI G ALGE+W+ I G++ YS Sbjct: 121 VIRSLISIPAGMTRMNFPIFIFLTTLGTILWNVILISFGVALGENWQQILGFMHVYS 177 >sp|Q48630|APL_LACLC Alkaline phosphatase like protein pir||S39339 alkaline phosphatase-like protein - Lactococcus lactis emb|CAA82306.1| alkaline phosphatase like protein [Lactococcus lactis] Length = 242 Score = 211 bits (537), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 104/179 (58%), Positives = 135/179 (75%), Gaps = 1/179 (0%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ I +M QFGY G+ ++ +ENIFPPIPSEVIL FGGFMTT S L I G++IA+T G Sbjct: 1 MQEIIIQVMNQFGYFGVAFLIMIENIFPPIPSEVILTFGGFMTTYSELGIIGMIIAATIG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVD-RYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMV 119 S+LGA ILY++GRLL V R+ER+V R GK +R+K DI KA+ WF K GY T+ FCR + Sbjct: 61 SVLGALILYFVGRLLSVERLERLVSGRLGKVLRLKPEDITKAEKWFLKRGYATIFFCRFI 120 Query: 120 PLVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 PL+RSLIS+PAG + MK F++ TT+GTL WN +L+ LGAALG++WE I G + +YSS Sbjct: 121 PLIRSLISVPAGSAKMKLPSFLILTTLGTLIWNIVLVSLGAALGDNWEMIAGILDSYSS 179 >ref|NP_266869.1| alkaline phosphatase [Lactococcus lactis subsp. lactis] sp|Q9CHL6|APL_LACLA Alkaline phosphatase like protein pir||A86714 alkaline phosphatase [imported] - Lactococcus lactis subsp. lactis (strain IL1403) gb|AAK04811.1| alkaline phosphatase [Lactococcus lactis subsp. lactis] Length = 214 Score = 211 bits (536), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 104/179 (58%), Positives = 135/179 (75%), Gaps = 1/179 (0%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+ I +M QFGY G+ ++ +ENIFPPIPSEVIL FGGFMTT S L I G++IA+T G Sbjct: 1 MQEIIIQVMNQFGYFGVAFLIMIENIFPPIPSEVILTFGGFMTTYSELGIIGMIIAATIG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVD-RYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMV 119 S+LGA ILY++GRLL V R+E++V R GK +R+K DI KA+ WF K GY T+ FCR + Sbjct: 61 SVLGALILYFVGRLLSVERLEKLVSGRLGKVLRLKPEDITKAEKWFLKRGYATIFFCRFI 120 Query: 120 PLVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 PL+RSLISIPAG + MK F++ TT+GTL WN +L+ LGAALG++WE I G + +YSS Sbjct: 121 PLIRSLISIPAGSAKMKLPSFLILTTLGTLIWNIVLVCLGAALGDNWEMIAGILDSYSS 179 >ref|NP_471318.1| similar to alkaline phosphatase [Listeria innocua] pir||AF1680 alkaline phosphatase homolog lin1984 [imported] - Listeria innocua (strain Clip11262) emb|CAC97214.1| lin1984 [Listeria innocua] Length = 207 Score = 204 bits (519), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 97/178 (54%), Positives = 133/178 (74%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+TWIT IM FGY GIFL++ +EN+FPPIPSE+IL FGGFMTT S L + V+I +T G Sbjct: 1 METWITSIMADFGYIGIFLLIMIENLFPPIPSEIILTFGGFMTTVSSLNVVMVIIVATLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA +LY + R+ IV +YG+ +R+K+SDI +A+++F KYG W V CRM+P Sbjct: 61 SVVGAILLYKVASYFGKERLTHIVLKYGRVLRLKESDIERAESFFLKYGSWAVFLCRMIP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 L+RSLISIPAGM+ MK F++ TT G+L WNT+LI LGA LGESW +I ++ ++S+ Sbjct: 121 LIRSLISIPAGMTKMKMSRFLILTTAGSLLWNTVLIGLGALLGESWGEIVVFMDSFST 178 >ref|NP_465395.1| similar to alkaline phosphatase [Listeria monocytogenes EGD-e] pir||AF1308 alkaline phosphatase homolog lmo1870 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99948.1| lmo1870 [Listeria monocytogenes] Length = 207 Score = 203 bits (517), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 95/178 (53%), Positives = 133/178 (74%) Query: 1 MKTWITDIMEQFGYAGIFLMLALENIFPPIPSEVILPFGGFMTTTSGLTIPGVLIASTAG 60 M+TWIT IM FGY GIF+++ +EN+FPPIPSE+IL FGGFMTT + L + V+I +T G Sbjct: 1 METWITSIMADFGYIGIFVLIMVENLFPPIPSEIILTFGGFMTTVTSLNVVMVIIVATLG 60 Query: 61 SLLGAAILYWIGRLLEVSRMERIVDRYGKWIRIKKSDIRKADAWFDKYGYWTVLFCRMVP 120 S++GA +LY + R+ +IV +YG+ +R+K+SDI +A+ +F KYG W V CRM+P Sbjct: 61 SVVGAILLYKVASYFGKERLTKIVLKYGRILRLKESDIERAENFFLKYGSWAVFLCRMIP 120 Query: 121 LVRSLISIPAGMSGMKFGLFMLFTTIGTLGWNTLLILLGAALGESWEDIGGYIGTYSS 178 L+RSLISIPAGM+ MK F++ TT G+L WNT+LI LGA LGESW +I ++ ++S+ Sbjct: 121 LIRSLISIPAGMTKMKMSKFLILTTAGSLLWNTVLIGLGAMLGESWSEIVVFMDSFST 178 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.143 0.455 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 284,787,022 Number of extensions: 10793342 Number of successful extensions: 39875 Number of sequences better than 1.0e-05: 244 Number of HSP's gapped: 39823 Number of HSP's successfully gapped: 244 Length of query: 207 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 87 Effective length of database: 431,425,698 Effective search space: 37534035726 Effective search space used: 37534035726 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 123 (52.0 bits)