BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0034.p (400 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060918.1| COG0436: Aspartate/tyrosine/aromatic aminotra... 346 7e-94 ref|NP_622832.1| PLP-dependent aminotransferases [Thermoanaeroba... 338 1e-91 ref|NP_622986.1| PLP-dependent aminotransferases [Thermoanaeroba... 332 7e-90 ref|NP_831324.1| Aspartate aminotransferase [Bacillus cereus ATC... 320 4e-86 ref|NP_655447.1| aminotran_1_2, Aminotransferase class I and II ... 320 5e-86 >ref|ZP_00060918.1| COG0436: Aspartate/tyrosine/aromatic aminotransferase [Clostridium thermocellum ATCC 27405] Length = 395 Score = 346 bits (887), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 157/384 (40%), Positives = 236/384 (61%), Gaps = 1/384 (0%) Query: 15 VQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNFTGYTDTGGI 74 + PS T +++ + + EG + G G+PDF TPD K AAI AI FT YT G Sbjct: 10 ISPSSTLAIDAKAKKMRSEGIDIIGFGAGEPDFDTPDHIKKAAIDAINAGFTKYTPASGT 69 Query: 75 LPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIPIPYWVSFPE 134 L L++AICR RD GL Y+P +IV++ G KH+L NA+ +C PGDEV+IP P WVS+PE Sbjct: 70 LELKQAICRKFKRDNGLDYNPSNIVISNGAKHSLVNALQAICNPGDEVIIPTPAWVSYPE 129 Query: 135 QVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVYSAAELAKLA 194 VK A PV++ G+K T + L + + TR +I+N P+NP+G +YS EL +A Sbjct: 130 MVKLADGVPVYIHCSEEEGFKFTIDKLEKAITDKTRAIIINSPSNPTGMIYSEEELRAVA 189 Query: 195 EFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYGMTGWRIGYS 253 + + +++ISDE+Y ++ + SIASF +++ T+V+N VSKSY MTGWRIGY+ Sbjct: 190 DLAVSKGIYIISDEIYEKLIYDGYKHVSIASFNDKIKDLTIVVNGVSKSYAMTGWRIGYT 249 Query: 254 AAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRRSVLVEGVRR 313 A+ + M +QSH TSNP +GPQ+ ++++ E+ RR +V+ + Sbjct: 250 ASNEQIAKIMANVQSHATSNPNSIAQKAALAALEGPQEIIDEMSAEFVKRRDYMVDRINS 309 Query: 314 IKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVMPGTGFGSPY 373 + G+ C P+GAFYV ++ S G+ + G I+ +D A+ LL+KA VA++PG+GFG+ Sbjct: 310 MNGVSCIKPNGAFYVMMNISKLIGKEIAGMKITGSDSFAEALLEKANVALVPGSGFGTDI 369 Query: 374 HIRLSYAVSRDEMESGIAAMESIL 397 H+RLSYA S + + G+ +E L Sbjct: 370 HVRLSYATSMENIVEGLNRIEKFL 393 >ref|NP_622832.1| PLP-dependent aminotransferases [Thermoanaerobacter tengcongensis] gb|AAM24436.1| PLP-dependent aminotransferases [Thermoanaerobacter tengcongensis] Length = 395 Score = 338 bits (868), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 167/395 (42%), Positives = 246/395 (62%), Gaps = 2/395 (0%) Query: 6 IQLADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNF 65 + L+ ++ PS T + + + + EG V G+G+PDF TPD K AAI+AI+ + Sbjct: 1 MNLSQNALQITPSMTLEITAKARQLKAEGVDVIDFGVGEPDFDTPDYIKEAAIEAIKKGY 60 Query: 66 TGYTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIP 125 T YT GIL L++AIC L R+ GL Y+P IVV+ G KH+++NA+ + PGDEV+IP Sbjct: 61 TKYTPASGILELKKAICEKLKRENGLFYEPEQIVVSNGAKHSIYNALSAILNPGDEVIIP 120 Query: 126 IPYWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVY 185 +PYW+S+PE V+ A KPVFVQT + +K+T E LT +N T+ LILN PNNP+GAVY Sbjct: 121 VPYWLSYPEMVRLAYGKPVFVQTKEENNFKITAEELTAAINPKTKALILNSPNNPTGAVY 180 Query: 186 SAAELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYG 244 + EL +AE + ++VISDEVY +++ E + SIAS ++E T+V+N +SK+Y Sbjct: 181 TRKELQDIAEVVEETGIFVISDEVYEKLIYEGE-HVSIASLGEKIKELTIVVNGMSKAYA 239 Query: 245 MTGWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRR 304 MTGWRIGY+A+ V M +QSHTTSNP G ++ + E+ RR Sbjct: 240 MTGWRIGYTASSLDVAKVMANIQSHTTSNPNSIAQYASVTALTGDGVAIKRMVEEFNKRR 299 Query: 305 SVLVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVM 364 VE + ++KGL+ P GAFYV+V+ + G+ + G+ I + D A +L+++A VAV+ Sbjct: 300 LYAVERISKMKGLKAVRPQGAFYVFVNIEEYVGKKVNGRKIKGSLDFATLLIEEANVAVV 359 Query: 365 PGTGFGSPYHIRLSYAVSRDEMESGIAAMESILGS 399 P FG +IR+SYA S + +E G+ +E+ L Sbjct: 360 PALPFGMDNYIRISYATSMENIEKGLDRIENFLNK 394 >ref|NP_622986.1| PLP-dependent aminotransferases [Thermoanaerobacter tengcongensis] gb|AAM24590.1| PLP-dependent aminotransferases [Thermoanaerobacter tengcongensis] Length = 395 Score = 332 bits (852), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 167/393 (42%), Positives = 247/393 (62%), Gaps = 2/393 (0%) Query: 6 IQLADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNF 65 + L+ ++ PS T + + + + +G V G+G+PDF TP K AAI AI+ + Sbjct: 1 MNLSQNALQITPSMTLEITAKAKQLKAQGVDVIDFGVGEPDFDTPSYIKEAAIDAIKRGY 60 Query: 66 TGYTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIP 125 T YT + GI L++AIC LL+D GLSY P IVV+ G KH+++NA+ + PGDEV+IP Sbjct: 61 TKYTPSSGIPELKKAICEKLLKDNGLSYTPEQIVVSNGAKHSIYNALSAILNPGDEVIIP 120 Query: 126 IPYWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVY 185 +PYW+S+PE V+ A KPVFV T + +K+T E L + T+ +ILN PNNPSGAVY Sbjct: 121 VPYWLSYPEMVRLAYGKPVFVHTKEENDFKITAEELENAITPKTKAIILNSPNNPSGAVY 180 Query: 186 SAAELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASF-PGMRERTVVINAVSKSYG 244 + EL ++A + +++VISDE+Y +++ E + SIASF ++E T+V+N +SK+Y Sbjct: 181 TKEELEEIARVVEEANIFVISDEIYEKLIYEGE-HVSIASFGEKIKELTIVVNGISKAYA 239 Query: 245 MTGWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRR 304 MTGWRIGY+A+ V M +QSHTTSNP G ++++E ++ E+ RR Sbjct: 240 MTGWRIGYTASSLEVAKVMSNIQSHTTSNPNSIAQYASVAALQGGEEEIEKMKEEFNRRR 299 Query: 305 SVLVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVM 364 +VE V +IK L+CS P GAFYV V+ + G+ L G+TI+ + D A L+ A VAV+ Sbjct: 300 LYMVERVNKIKDLKCSTPKGAFYVMVNIEKYIGRELNGKTINGSLDFASALIDGANVAVV 359 Query: 365 PGTGFGSPYHIRLSYAVSRDEMESGIAAMESIL 397 P FG +IR+SYA S + ++ G+ +E L Sbjct: 360 PALPFGMDNYIRMSYATSLENIKKGLDRIEDFL 392 >ref|NP_831324.1| Aspartate aminotransferase [Bacillus cereus ATCC 14579] gb|AAP08525.1| Aspartate aminotransferase [Bacillus cereus ATCC 14579] Length = 395 Score = 320 bits (819), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 158/396 (39%), Positives = 238/396 (60%), Gaps = 5/396 (1%) Query: 6 IQLADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNF 65 ++LA V + PS T + + + EG V LG G+PDF TP+ AA +A+ + Sbjct: 1 MKLAKRVAALTPSSTLEITAKAQALKAEGHDVIGLGAGEPDFNTPEHIMDAAHKAMLEGH 60 Query: 66 TGYTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIP 125 T YT TGG+ L++ I + RD G++YDP +I+V G KHAL+ L GDEV+IP Sbjct: 61 TKYTPTGGLQSLKQEIVKKFTRDQGIAYDPSEIIVCNGAKHALYTLFQVLLDEGDEVIIP 120 Query: 126 IPYWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVY 185 PYWVS+PEQVK AG KPV+V+ + + YK+T + L + E T+ +I+N P+NP+G +Y Sbjct: 121 TPYWVSYPEQVKLAGGKPVYVEGLEDNEYKITAKQLREAITEKTKAVIINSPSNPTGMIY 180 Query: 186 SAAELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASFPG-MRERTVVINAVSKSYG 244 S EL +L E C + D+ ++SDE+Y ++ YTSIA ++E+T++IN VSKS+ Sbjct: 181 SKEELQQLGEVCLEHDILIVSDEIYEKLIYGGVEYTSIAQLSNALKEQTLIINGVSKSHS 240 Query: 245 MTGWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRR 304 MTGWRIGY+A + AM L SH+TSNP G Q+ +E +R + R Sbjct: 241 MTGWRIGYAAGNKQLIKAMTNLASHSTSNPTSIAQYGAIAAYTGSQEPVETMRQAFEERL 300 Query: 305 SVLVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVM 364 +++ + + +I G C P GAFY++ + L G +T DD A LL++ VA++ Sbjct: 301 NIIYDKLIQIPGFTCIKPQGAFYLFPNVK--EAVALSG--YATVDDWAKALLEEEKVALV 356 Query: 365 PGTGFGSPYHIRLSYAVSRDEMESGIAAMESILGSK 400 PGTGFG+P ++RLSYA S +++E + + + + SK Sbjct: 357 PGTGFGAPNNVRLSYATSLEQVEKALERIHTFMKSK 392 >ref|NP_655447.1| aminotran_1_2, Aminotransferase class I and II [Bacillus anthracis A2012] ref|NP_844018.1| aspartate aminotransferase [Bacillus anthracis str. Ames] gb|AAP25504.1| aspartate aminotransferase [Bacillus anthracis str. Ames] Length = 395 Score = 320 bits (819), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 158/396 (39%), Positives = 237/396 (59%), Gaps = 5/396 (1%) Query: 6 IQLADWVERVQPSPTALLESTVARYQREGRTVYRLGLGQPDFPTPDRAKAAAIQAIQDNF 65 ++LA V + PS T + + + EG V LG G+PDF TP+ AA +A+ + Sbjct: 1 MKLAKRVAALTPSATLEITAKAQALKAEGHDVIGLGAGEPDFNTPEHIMDAAHKAMLEGH 60 Query: 66 TGYTDTGGILPLREAICRALLRDMGLSYDPGDIVVTVGGKHALFNAICTLCRPGDEVLIP 125 T YT TGG+ L++ I + RD G++YDP +I+V G KHAL+ L GDEV+IP Sbjct: 61 TKYTPTGGLQALKQEIVKKFTRDQGIAYDPSEIIVCNGAKHALYTLFQVLLDEGDEVIIP 120 Query: 126 IPYWVSFPEQVKFAGAKPVFVQTVASSGYKVTPETLTPWVNESTRLLILNQPNNPSGAVY 185 PYWVS+PEQVK AG KPV+V+ + + YK+T E L + E T+ +I+N P+NP+G +Y Sbjct: 121 TPYWVSYPEQVKLAGGKPVYVEGLEGNEYKITAEQLREAITEKTKAVIINSPSNPTGMIY 180 Query: 186 SAAELAKLAEFCRQRDLWVISDEVYSAFVFKPEGYTSIASFP-GMRERTVVINAVSKSYG 244 S EL +L E C + D+ ++SDE+Y ++ YTSIA ++E+T++IN VSKS+ Sbjct: 181 SKEELQQLGEVCLEHDILIVSDEIYEKLIYGGAEYTSIAQLSNALKEQTLIINGVSKSHS 240 Query: 245 MTGWRIGYSAAPAAVTGAMLRLQSHTTSNPXXXXXXXXXXXXDGPQDDLEDIRGEYATRR 304 MTGWRIGY+A + AM L SH+TSNP G Q+ +E +R + R Sbjct: 241 MTGWRIGYAAGNKQLIKAMTNLASHSTSNPTSIAQYGAIAAYAGSQEPVETMRQAFEERL 300 Query: 305 SVLVEGVRRIKGLECSVPDGAFYVWVDASAWRGQTLEGQTISTADDLADILLKKAGVAVM 364 +++ + + +I G C P GAFY++ + L G T D+ A LL++ VA++ Sbjct: 301 NIIYDKLIQIPGFTCIKPQGAFYLFPNVK--EAVALSG--YETVDEWAKALLEEEKVALV 356 Query: 365 PGTGFGSPYHIRLSYAVSRDEMESGIAAMESILGSK 400 PGTGFG+P ++RLSYA S +++E + + + + SK Sbjct: 357 PGTGFGAPNNVRLSYATSLEQVEKALERIHTFMKSK 392 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 595,661,317 Number of extensions: 24479822 Number of successful extensions: 64187 Number of sequences better than 1.0e-05: 3033 Number of HSP's gapped: 59673 Number of HSP's successfully gapped: 3034 Length of query: 400 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 272 Effective length of database: 409,704,906 Effective search space: 111439734432 Effective search space used: 111439734432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)