BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0013.p (709 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00045566.1| COG2931: RTX toxins and related Ca2+-binding ... 92 4e-17 gb|EAD58774.1| unknown [environmental sequence] 69 3e-10 ref|ZP_00075268.1| hypothetical protein [Trichodesmium erythraeu... 67 2e-09 ref|ZP_00112416.1| COG2931: RTX toxins and related Ca2+-binding ... 67 2e-09 ref|ZP_00071725.1| COG2931: RTX toxins and related Ca2+-binding ... 66 3e-09 >ref|ZP_00045566.1| COG2931: RTX toxins and related Ca2+-binding proteins [Magnetococcus sp. MC-1] Length = 11699 Score = 92.4 bits (228), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 103/353 (29%), Positives = 155/353 (43%), Gaps = 53/353 (15%) Query: 38 FDTVMEDTLEPA-GQSVSVINSN----------GIGITEVDNTNGQWQYSAG-GNFWLSI 85 D V ED+ P G VS + S+ GI +T VD ++GQWQYS G W I Sbjct: 7531 IDDVQEDSTVPQNGTMVSNLLSDIYYDVDGTLSGIALTSVDESHGQWQYSWDYGATWQGI 7590 Query: 86 VAPDPGKIAVFGRDVMIRFVPKKDWNGTAQIRYRGWLSSGEQSSNYV---NVNAEYTGDA 142 + + IRF+P D+ G++ +RGW + S N V +V+A G Sbjct: 7591 DGLSESQALLLESSDSIRFIPNADYAGSSTFTFRGW----DTSDNGVAGTSVDATQNGGT 7646 Query: 143 ESYSESVQAAQIVVTPVNDAPYISELSGSTYLDFDGVGYVTIPDLNI-YSNSLTIETWVN 201 ++S Q+ V VND +S ++ S L FDGV V +++ S+S T+E WV Sbjct: 7647 TAFSAVSHTGQVNVIEVNDPSQLSAVAPSQVLSFDGVDDVASAAISLPPSSSFTLEAWVY 7706 Query: 202 ASSAPTWARIFDTSY-GPDNFNLHLTFEGSTGRLALEALPQKGPRVKSYLVRTTEQLPLN 260 +S + IF G F++ F+ S G+L + V + + + LN Sbjct: 7707 RNSGSSDDTIFSQGVEGTGTFSVG--FDAS-GQLNWN-IDSDTLSVNTADIDSNNVGMLN 7762 Query: 261 QWVHVAAVYNAVEKKAY--IYWNGILKGSGFMDLTDMAKGSAANSNIQRPYNYIGESTWS 318 QW HVAAVY+ + +Y +GI +A+ + S Y+G +T Sbjct: 7763 QWYHVAAVYDDMGGSPVRTLYVDGI----------QVAQSTTGPS-------YVGGNTLV 7805 Query: 319 QDANYVGG--------MRDYRIWNKAKTQAEIESQMNTSLTGTETNLMVNYKF 363 + GG + D RIW+ A+T EI N +T T TNL + F Sbjct: 7806 LGSYLAGGSGQDFDGKLDDVRIWSDARTAQEIAESANGVITET-TNLTTYFDF 7857 Score = 82.4 bits (202), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 90/323 (27%), Positives = 134/323 (41%), Gaps = 47/323 (14%) Query: 79 GNFWLSIVAPDPGKIAVFGRDVMIRFVPKKDWNGTAQIRYRGWLSSGEQSSNYVN----V 134 G WL I A G + V + + K + Y +SGE SNY + V Sbjct: 10182 GTLWLDINAN--GSLDVGTEEAFAGSIVTKAQLDAGLLHYTP--NSGESGSNYTSFSYYV 10237 Query: 135 NAEYTGDAESYSESVQAAQIVVTPVNDAP--YISELSGSTYLDFDGVGYVTIPDLNIYSN 192 N T A + + +V AP Y + G+ +T+ +NI ++ Sbjct: 10238 NDGTTNSAIANTITVDITTGSTGTTGSAPVVYTAAHQGAMQFSTGEADTLTVTGVNIANS 10297 Query: 193 SLTIETWVNASSAPT-WARIFDTS------------YGPDNFNLHLTF-EGSTGRLALEA 238 S T+E WVN S++ + W +F +GP+N LT G+TGR++ + Sbjct: 10298 SYTLEAWVNRSTSTSHWDMLFTQGTTTTDGQYQFFGFGPNN---DLTMGHGATGRISADI 10354 Query: 239 LPQKGPRVKSYLVRTTEQLPLNQWVHVAAVYNAVEKKAYIYWNGILKGSGFMDLTDMAKG 298 +G S LV +W HVAA Y+ + IY NG+L+ SG + Sbjct: 10355 SNFEG---TSNLV--------GEWYHVAASYDVTSGEGNIYINGVLQASGILGF------ 10397 Query: 299 SAANSNIQRPYNYIGESTWSQDANYVGGMRD-YRIWNKAKTQAEIESQMNTSLTGTETNL 357 A +N P IG STW+ + + G+ D RIW+ +T EI N + E NL Sbjct: 10398 --AFTNAADPDMSIGNSTWAAGIDGLDGLLDQVRIWSDVRTAQEIAESYNQAWGSNEENL 10455 Query: 358 MVNYKFNNPDDGAVAKDSSAGQR 380 + NY FN D V S+ G Sbjct: 10456 LANYDFNIVADNTVFDSSNNGNH 10478 Score = 67.4 bits (163), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 66/216 (30%), Positives = 103/216 (47%), Gaps = 22/216 (10%) Query: 154 IVVTPVNDAPYISELSGSTYLDFDGVGYVTIPDLNIY-SNSLTIETWVNASSAPTWARIF 212 + + PV+DAP +S + S L FDGV + L + S S T+E WV +S+ + IF Sbjct: 9141 LAIAPVDDAPALSAVQPSQVLSFDGVDDMASATLALAPSTSFTLEGWVYRNSSDSSDAIF 9200 Query: 213 DTSYGPDNFNLHLTFEGSTGRLALEALPQKGPRVKSYLVRTTEQLPLNQWVHVAAV--YN 270 S G + + +TG L+ + V + + + +NQW HVAAV YN Sbjct: 9201 --SQGFEGTGTFVVGFDATGHLSWN-IDSDTLSVNTADIDSNNVGMVNQWYHVAAVYDYN 9257 Query: 271 AVEKKAYIYWNGILKGSGFMDLTDMAKGSAANSNIQRPYNYIGE---STWSQDANYVGGM 327 A +Y +G+ +A+ +A NS IG S+ QD + G + Sbjct: 9258 AGAPVRTLYVDGV----------QVAQSTAGNSYAGGSTLVIGNYLASSSGQD--FDGKL 9305 Query: 328 RDYRIWNKAKTQAEIESQMNTSLTGTETNLMVNYKF 363 D RIW+ ++ AEI++ N +++ T TNLM Y F Sbjct: 9306 DDVRIWSDVRSAAEIQNSANGAVSDT-TNLMAYYTF 9340 Score = 55.5 bits (132), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 55/237 (23%), Positives = 94/237 (39%), Gaps = 32/237 (13%) Query: 157 TPVNDAPYISELSGSTYLDFDGVGYVTIPDLNIYSNSLTIETWVNASSAPTWARIF---D 213 T AP + + + FDG + +++ + S T+E WVN SA + Sbjct: 10979 TATGSAPVVYAAAHQGVMQFDGDDQIVAEGVSVANRSYTLEAWVNRQSADGTVDVVMAQG 11038 Query: 214 TSYGPDNF---------NLHLTFEGSTGRLALEALPQKGPRVKSYLVRTTEQLPLNQWVH 264 + G D L L +GS + P + + W H Sbjct: 11039 ATLGADQVMWFGFDSDQTLSLYHDGSGVSFDISGYNTAYPNM------------VGAWNH 11086 Query: 265 VAAVYNAVEKKAYIYWNGILKGSGFMDLTDMAKGSAANSNIQRPYNYIGESTWSQDANYV 324 +AA ++ +A +Y NG G+ M +D+ +A SN+ +IG W+ A+ Sbjct: 11087 IAASFDDTTNEATLYINGTQVGTSAMLSSDLVD--SAGSNL-----FIGNDEWTGAADGF 11139 Query: 325 GGMRD-YRIWNKAKTQAEIESQMNTSLTGTETNLMVNYKFNNPDDGAVAKDSSAGQR 380 GM D R+W ++ EI N + + NL+ +Y FN V ++S++G Sbjct: 11140 SGMVDTVRVWGDVRSAQEIADSYNQAWGSNDDNLLASYDFNIVAGNTVLENSNSGNH 11196 >gb|EAD58774.1| unknown [environmental sequence] Length = 304 Score = 69.3 bits (168), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 74/233 (31%), Positives = 109/233 (46%), Gaps = 34/233 (14%) Query: 168 LSGSTY-LDFDGVG-YVTIPD---LNIYSNSLTIETWVNASSAPTWARIFDTSY--GPDN 220 L+G Y L+FDG Y+ IPD L++ +N TIE W+ S A I + + Sbjct: 5 LNGQDYSLNFDGNNVYILIPDNASLDLTTN-YTIEAWIFPESFSWLAGIVSKYHTNAANG 63 Query: 221 FNLHLTFEGSTGRLALEALPQKGPRVKSYLVRTTEQLPLNQWVHVAAVYNAVEKKAYIYW 280 + L LTF+ L + V T L NQW HVAAV N +K Y+ Sbjct: 64 YMLRLTFQAPYTGLGFDER-----------VTNTGILSSNQWYHVAAVNNNGSRKLYV-- 110 Query: 281 NGILKGSGFMDLTDMAKGSAANSNIQRPYNYIGESTWSQDANYVGGMRDYRIWNKAKTQA 340 NG+ +L+ A +AN+N R IG S+ + G + + RIWN A+ Q Sbjct: 111 NGVE-----YNLSGSALNVSANNNPLR----IGSDYGSR--YFDGRIDEVRIWNIARGQD 159 Query: 341 EIESQMNTSLTGTETNLMVNYKFNNPDDGAVAKDSSAGQRNGNIISG-KWLSG 392 +I M++ L+G+E L+ Y FN G + D + NG ++ G +W SG Sbjct: 160 DIVETMDSVLSGSEAGLVAYYSFNEG-SGIILNDQTGNGHNGTLVGGPQWSSG 211 >ref|ZP_00075268.1| hypothetical protein [Trichodesmium erythraeum IMS101] Length = 201 Score = 66.6 bits (161), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 39/110 (35%), Positives = 60/110 (54%), Gaps = 5/110 (4%) Query: 58 SNGIGITEVDNTNGQWQYSAG-GNFWLSIVAPDPGKIAVFGRDVMIRFVPKKDWNGTAQI 116 S GI ITE+DN+ G W+YS G+ W+ + + D +RFVP+ D+ G I Sbjct: 86 SKGIAITEIDNSKGIWEYSVDDGDNWVEVSNSSTTLLTATDSD-RLRFVPETDFFGKKSI 144 Query: 117 RYRGWLSSGEQSSNYVNVNAEYTGDAESYSESVQAAQIVVTPVNDAPYIS 166 ++RGW ++ S+ TGD ++SE++ +A I+ VND P IS Sbjct: 145 KFRGWDTTDGSSNTTQLTEVLATGDTNAFSENIGSANIL---VNDIPEIS 191 >ref|ZP_00112416.1| COG2931: RTX toxins and related Ca2+-binding proteins [Nostoc punctiforme] Length = 1241 Score = 66.6 bits (161), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 38/102 (37%), Positives = 54/102 (52%), Gaps = 4/102 (3%) Query: 60 GIGITEVDNTNGQWQYSA-GGNFWLSIVAPDPGKIAVFGRDV-MIRFVPKKDWNGTAQIR 117 GI + VDNT+G WQYS GGN W++ A RD +RF+P +NGTA+I Sbjct: 57 GIAVIGVDNTHGIWQYSTNGGNNWINFAVS--AISATLLRDTEKVRFIPNAGYNGTAEIT 114 Query: 118 YRGWLSSGEQSSNYVNVNAEYTGDAESYSESVQAAQIVVTPV 159 +R W +S +S VN G +YS + + A I + P+ Sbjct: 115 FRAWDASDGNASGTTGVNPGVGGGTTAYSSATETASISILPL 156 >ref|ZP_00071725.1| COG2931: RTX toxins and related Ca2+-binding proteins [Trichodesmium erythraeum IMS101] Length = 2145 Score = 66.2 bits (160), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 39/110 (35%), Positives = 60/110 (54%), Gaps = 5/110 (4%) Query: 58 SNGIGITEVDNTNGQWQYSAG-GNFWLSIVAPDPGKIAVFGRDVMIRFVPKKDWNGTAQI 116 S GI ITE+DN+ G W+YS G+ W+ + + D +RFVP+ D+ G I Sbjct: 1407 SKGIAITEIDNSKGIWEYSVDDGDNWVEVSNSSTTLLTATDSD-RLRFVPETDFFGKKSI 1465 Query: 117 RYRGWLSSGEQSSNYVNVNAEYTGDAESYSESVQAAQIVVTPVNDAPYIS 166 ++RGW ++ S+ TGD ++SE++ +A I+ VND P IS Sbjct: 1466 KFRGWDTTDGSSNTTQLTEVLATGDTNAFSENIGSANIL---VNDIPEIS 1512 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.130 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,007,290,241 Number of extensions: 41143421 Number of successful extensions: 73789 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 73784 Number of HSP's successfully gapped: 64 Length of query: 709 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 576 Effective length of database: 396,129,411 Effective search space: 228170540736 Effective search space used: 228170540736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)