BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0128.0011.p (462 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097676.1| COG2931: RTX toxins and related Ca2+-binding ... 167 7e-40 gb|AAP48997.1| cellulosomal bifunctional enzyme-binding/scaffold... 155 3e-36 ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clo... 154 3e-36 ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellu... 153 9e-36 ref|ZP_00061799.1| hypothetical protein [Clostridium thermocellu... 149 1e-34 >ref|ZP_00097676.1| COG2931: RTX toxins and related Ca2+-binding proteins [Desulfitobacterium hafniense] Length = 1549 Score = 167 bits (422), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 86/242 (35%), Positives = 141/242 (58%), Gaps = 6/242 (2%) Query: 4 RITVKVESGQLGLGSVLAETVIQRTKDSGGILHDVVNFTMDRVDEVVRKITGTLDNTARI 63 +ITV V+ G S +A+ ++R S G D V + + E V+K+ +TARI Sbjct: 998 QITVDVKQGNTD--STVAQITVERRTGSDGKKTDTVTYQESKAAETVKKLKEENKDTARI 1055 Query: 64 MIPDEKDQVADINVNVPKDAIKTLGDSNVNLEIFTDNVRILIAQSSLDTFTE----DLFF 119 +IPDEKD+VA+ VN+P A++ L +NL+I T+ +I I+++++ ++ DL+F Sbjct: 1056 VIPDEKDEVAETKVNIPVQALEVLAQGGINLQIDTEEAKIDISKNTITGISQEVEGDLYF 1115 Query: 120 HVVPIKDQSLRKXXXXXXXXXXXXXXXXXXKQIDVVARPMTIETNMQSRPVTITLPLRDV 179 +VP+KD++ +K + +V P+TIETNM S ITLPL + Sbjct: 1116 RLVPVKDEAQKKSTIDQALLKAALVSGNTDSSLSIVGNPVTIETNMSSTEADITLPLTGM 1175 Query: 180 QLPATLPEREDFLSNLVIFIEHSDGDKELKKAKIVDYKPGELGLQFNVDKFSTFTILNME 239 LP+ E+ L L ++IEHSDGDKEL + ++V+YK G G++F+++KFS FT++ + Sbjct: 1176 TLPSNPAEKAALLQQLAVYIEHSDGDKELVQGELVEYKEGVYGIRFHINKFSIFTVVRTD 1235 Query: 240 GW 241 + Sbjct: 1236 AF 1237 >gb|AAP48997.1| cellulosomal bifunctional enzyme-binding/scaffoldin-anchoring protein D; ScaD [Acetivibrio cellulolyticus] Length = 852 Score = 155 bits (391), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 81/200 (40%), Positives = 112/200 (56%), Gaps = 4/200 (2%) Query: 262 KAFITGYSDGTFKPNHSITRAEMAAILARNLGYDASAAPAVSSYPDVRDSHWAKGAIEFV 321 K+++ GY+D F+P ITRAE A I+A+ + +SA ++PDV + HWAK +IE V Sbjct: 654 KSYLKGYTDNCFRPKKEITRAEAAVIIAKFVDVGSSANSNNVNFPDVTNKHWAKESIELV 713 Query: 322 KEAGLMQGNEKGRFLPDAPISRGEMAAIASRYKKLDTTAATS-GGGFKDAAGHWAAKDIE 380 + GL +G G F PD I RGE AA+ Y LD A+T F D HWA I Sbjct: 714 TKVGLFKGYPDGSFKPDQTIKRGEFAAVI--YNLLDLDASTGLSNNFTDIDDHWAKSFIL 771 Query: 381 AASKAGIINGYEDGTYRPGGNLTRAEAVKVVNRLFGRGPLYGAATPSWPDEPATNWAYNE 440 + INGY DGT++P + R E V +VNR RGPL G AT + D P WA+ + Sbjct: 772 KLANLRYINGYSDGTFKPDNKIKRDECVVLVNRALNRGPLNG-ATLDFTDVPKNYWAFKD 830 Query: 441 IEEASIDHTFTVRNEGGETL 460 I E S+DH + + ++ E + Sbjct: 831 IAEGSLDHKYYINSDEEEVI 850 >ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clostridium thermocellum ATCC 27405] sp|Q06853|SLP2_CLOTM CELL SURFACE GLYCOPROTEIN 2 PRECURSOR (S-LAYER PROTEIN 2) pir||T18263 S-layer protein - Clostridium thermocellum emb|CAA47842.1| S-layer protein [Clostridium thermocellum] Length = 688 Score = 154 bits (390), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 82/202 (40%), Positives = 114/202 (56%), Gaps = 5/202 (2%) Query: 263 AFITGYSDGTFKPNHSITRAEMAAILARNLGYDASAAPAVS-SYPDVRDSHWAKGAIEFV 321 +++TGY D F+P SITRAE A I A+ LG + + + SY DV SHWA AI+FV Sbjct: 482 SYLTGYPDKMFRPEKSITRAEAAVIFAKLLGANENTKINYNVSYTDVDSSHWASWAIKFV 541 Query: 322 KEAGLMQGNEKGRFLPDAPISRGEMAAIASRY---KKLDTTAATSGGGFKDAAGHWAAKD 378 L G G F P+ I+R E + + + +K F D GHWA + Sbjct: 542 SYKKLFTGYPDGSFKPNQNITRAEFSTVVFKLLVSEKGLKEEKIEKSKFGDTKGHWAQQF 601 Query: 379 IEAASKAGIINGYEDGTYRPGGNLTRAEAVKVVNRLFGRGPLYGAATPSWPDEPATNWAY 438 IE S G INGY DGT++P N+ R+E+V ++NR GRGPL+GA + D P T+WA+ Sbjct: 602 IEQLSDLGYINGYPDGTFKPNNNIKRSESVALINRAMGRGPLHGAPQ-VFEDVPQTHWAF 660 Query: 439 NEIEEASIDHTFTVRNEGGETL 460 +I E ++H + + NEG E L Sbjct: 661 KDIAEGVLNHRYKLDNEGKEQL 682 Score = 60.5 bits (145), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 43/130 (33%), Positives = 60/130 (46%), Gaps = 22/130 (16%) Query: 262 KAFITGYSDGTFKPNHSITRAEMAAILARNLGYDASAAP---AVSSYPDVRDSHWAKGAI 318 K TGY DG+FKPN +ITRAE + ++ + L + S + D + HWA+ I Sbjct: 544 KKLFTGYPDGSFKPNQNITRAEFSTVVFKLLVSEKGLKEEKIEKSKFGDTK-GHWAQQFI 602 Query: 319 EFVKEAGLMQGNEKGRFLPDAPISRGEMAAIASRYKKLDTTAATSGGG--------FKDA 370 E + + G + G G F P+ I R E A+ +R G G F+D Sbjct: 603 EQLSDLGYINGYPDGTFKPNNNIKRSESVALINR---------AMGRGPLHGAPQVFEDV 653 Query: 371 -AGHWAAKDI 379 HWA KDI Sbjct: 654 PQTHWAFKDI 663 >ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellum ATCC 27405] sp|Q06848|ANCA_CLOTM Cellulosome anchoring protein precursor pir||T18264 cellulosome anchoring protein - Clostridium thermocellum emb|CAA47843.1| cellulosome anchoring protein [Clostridium thermocellum] Length = 447 Score = 153 bits (386), Expect = 9e-36, Method: Compositional matrix adjust. Identities = 85/206 (41%), Positives = 113/206 (54%), Gaps = 10/206 (4%) Query: 264 FITGYSDGTFKPNHSITRAEMAAILARNLGYDASAAPAVSS--YPDVRDSHWAKGAIEFV 321 F+ GY G FKP ++ITRAE A I A+ LG D ++A SS + D++DSHWA AI++V Sbjct: 242 FLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAIKYV 301 Query: 322 KEAGLMQGNEKGRFLPDAPISRGEMAAIASRYKKLDTTAATSGGG-----FKDAAGHWAA 376 E L G G F+PD I+R E A + YK L+ G KD GHWA Sbjct: 302 TEQNLFGGYPDGTFMPDKSITRAEFATVT--YKFLEKLGKIEQGTDVKTQLKDIEGHWAQ 359 Query: 377 KDIEAASKAGIINGYEDGTYRPGGNLTRAEAVKVVNRLFGRGPLYGAATPSWPDEPATNW 436 K IE G I GY D T+RP ++ RAE+V ++NR RGPL GA + D P W Sbjct: 360 KYIETLVAKGYIKGYPDETFRPQASIKRAESVALINRSLERGPLNGAVL-EFTDVPVNYW 418 Query: 437 AYNEIEEASIDHTFTVRNEGGETLSQ 462 AY +I E I H++ + G E + + Sbjct: 419 AYKDIAEGVIYHSYKIDENGQEVMVE 444 >ref|ZP_00061799.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 631 Score = 149 bits (376), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 77/199 (38%), Positives = 114/199 (57%), Gaps = 2/199 (1%) Query: 262 KAFITGYSDGTFKPNHSITRAEMAAILARNLGYDASAAPAVSSYPDVRDSHWAKGAIEFV 321 K++I GY D +FKP++ ITRAE+A ILA +G + + DV +HWA I+ V Sbjct: 433 KSYINGYPDNSFKPDNHITRAEIATILANVMGLSVPDNIEETGFKDVDKNHWAARYIKAV 492 Query: 322 KEAGLMQGNEKGRFLPDAPISRGEMAAIASRYKKLDTTAATSGGGFKDAAGHWAAKDIEA 381 +AG+ +G E F P+ ISR E+A + + LD A F D GHWA+ IE Sbjct: 493 TDAGIFKGYEDSTFRPNETISRAELATVIFKCLDLDEKKAVK-SSFTDTKGHWASDFIEE 551 Query: 382 ASKAGIINGYEDGTYRPGGNLTRAEAVKVVNRLFGRGPLYGAATPSWPDEPATNWAYNEI 441 S+ II GYEDG+++P G +TRAEAV ++N + R P+ + + D +WA+ I Sbjct: 552 VSRNKIIRGYEDGSFKPNGKVTRAEAVVMINNMLYRDPI-NVDSSKFTDLDKKHWAFGHI 610 Query: 442 EEASIDHTFTVRNEGGETL 460 E A+ D+ F + +GG+ L Sbjct: 611 EAAAGDYEFKIDEDGGKVL 629 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 627,638,368 Number of extensions: 25083884 Number of successful extensions: 66529 Number of sequences better than 1.0e-05: 134 Number of HSP's gapped: 66125 Number of HSP's successfully gapped: 223 Length of query: 462 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 333 Effective length of database: 406,989,807 Effective search space: 135527605731 Effective search space used: 135527605731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)