BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0021.p (534 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244709.1| two-component response regulator [Bacillus halo... 127 7e-28 ref|NP_242776.1| two-component response regulator [Bacillus halo... 122 3e-26 ref|NP_801837.1| putative two-component response regulator [Stre... 110 1e-22 ref|NP_607656.1| putative two-component sensor response regulato... 109 2e-22 ref|NP_665090.1| putative two-component sensor response regulato... 109 2e-22 >ref|NP_244709.1| two-component response regulator [Bacillus halodurans] pir||B84130 two-component response regulator BH3842 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07561.1| two-component response regulator [Bacillus halodurans] Length = 530 Score = 127 bits (319), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 132/543 (24%), Positives = 258/543 (47%), Gaps = 60/543 (11%) Query: 4 NLLIVDDEENIRLGIRAMLEREYPGMYRYFFAEDGEEALQQLNQAAFDIMMTDIRMPVMD 63 N+LIVDDE+ R G++ L ++ P ++ A +G +A+++ + D+MM DI+MP +D Sbjct: 3 NVLIVDDEQIEREGLKLFL-KDRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGVD 61 Query: 64 GITLIGRVQQLEHRPVVVIISGYDDFEYAKQAIRFGVRDYLLKPIVRSELRGTFSRL--- 120 G+ + ++Q +++S +D FEYA++ +R+GV++YLLKP + ++ R+ Sbjct: 62 GVQAVKEIKQQLPETEFIMVSAFDTFEYAREVMRYGVKEYLLKPTTKEDVLTALDRIVGE 121 Query: 121 -EQALKREQDINGMLSKATQREQDYRQNEMYYILMNIEIAGPELSERLDRAGLEDLQAGY 179 EQA + + D + + Q + + Y+LM+ I E E L Q GY Sbjct: 122 IEQAREEKNDQYKLQQRLEQISSFVQTESILYLLMD-HIQDYEWMEW--NEWLRTEQEGY 178 Query: 180 --VIGLIQEAEANGRTE------------GGGVLYSRIHEF----LHTEALAEEYHFLDK 221 V Q+ + +TE G + S + F L L E + + K Sbjct: 179 AVVFSFHQQGKEIRKTEKQQWHARIKKEIGLQLPKSTVGPFIGHHLPILMLTENENGMTK 238 Query: 222 EGRSLVVIRDEQRLGKLLEYLGQDRLLACQIGSSERMDKAEQFKTAYAQALKALRYFFLK 281 + R VVI Q L K+ + + Q + +G R+ F T+Y +AL AL + Sbjct: 239 QEREQVVI---QLLHKVKQAV-QPAKVKTGVGGIYRL---HFFSTSYREALLALDIIYDH 291 Query: 282 SSTGLIRYSQIKDKPRNFEIPTEEIVKIGNMLGTGR-EQEMQAL---LSQVLDYRKMLLY 337 Y + + ++P E K+ + +G E+ +QA + +L K L+ Sbjct: 292 KQASYYFYDKNVKIRADEQLPIERENKLIEAIKSGDGEKALQAFDHYVQAILRCSKHPLH 351 Query: 338 DI-SYMEEISTAINELIFDKAFNVYGKEAVEIIKVYRQIGYLYNSPHFQDYYHGVENLLN 396 D+ Y++++ + L+ ++ + A + + +++ H Q HG +L+ Sbjct: 352 DMRKYLQQLFIILGRLL--NEMGLHWETAQDFAR--------FSTVH-QVTEHGKSEILH 400 Query: 397 RMNDFIKGIKSAHLNQREMKAALLFIEQNYNRSDF----NLAMVSNHVSFNYSYFSSAFK 452 ++ ++ + +L+ + ++Y F L ++ H+ + Y S FK Sbjct: 401 ----IVRTLQEWRTTDEQ---SLMLLARDYVHDHFREPLTLDDLAKHIGLSPYYVSKLFK 453 Query: 453 EYMGMSFSQYIKKLRLVKAKELLENSAYKVYEVSAKVGFENPKHFNKVFREAEGISPLEY 512 E ++FS+Y+ +LR+ AK+LL + + E++ ++G+++P +F++VF++ G SP +Y Sbjct: 454 ERFDLTFSEYVTQLRVEHAKQLLSLTQKSLKEITYEIGYKDPNYFSRVFKKLTGKSPSDY 513 Query: 513 RMA 515 R + Sbjct: 514 RKS 516 >ref|NP_242776.1| two-component response regulator [Bacillus halodurans] pir||F83888 two-component response regulator BH1910 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05629.1| two-component response regulator [Bacillus halodurans] Length = 506 Score = 122 bits (305), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 118/526 (22%), Positives = 235/526 (44%), Gaps = 49/526 (9%) Query: 4 NLLIVDDEENIRLGIRAMLEREYPGMYRYFFAEDGEEALQQLNQAAFDIMMTDIRMPVMD 63 +++IVDDE +R G++A++ E G A +G+E + Q + D+M+ DIRMP +D Sbjct: 3 DVMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPDLMIVDIRMPEVD 62 Query: 64 GITLIGRVQQLEHRPVVVIISGYDDFEYAKQAIRFGVRDYLLKPIVRSELRGTFSRLEQA 123 G++LI ++ + +I+SG+ DF YAK+A+ DYLLKP+ EL + ++ Sbjct: 63 GLSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKVYGR 122 Query: 124 LKREQDINGMLSKATQREQDYRQNEMYYILMNIEIAGPELSERLDRAGLEDLQAGYVIGL 183 + R ++ ++ + +++ + E+ + G LSE Y + L Sbjct: 123 ITRHHQVHELVERVQEKQVEMVLTEL--------LEGTPLSEIPIGKEHNFFATDYQVAL 174 Query: 184 IQEAEANGRTEGGGVLYSR----IHEFLHTEALAEEYHFLDKEGRSLVVIRDEQRLGKLL 239 ++ + + ++ + + F + + FL K G+ + R Sbjct: 175 VENRQDRLTKQLKRLIEEQEEGYVIPFPEYIVIVCKNRFLQKRGK-------DHRYHLFA 227 Query: 240 EYLGQDRLLACQIGSSERMDKAEQFKTAYAQALKALRYFFLKSSTGLIRYSQIKD--KPR 297 ++L ++ L AC S+ ++ A+ +++ Y A + F L Q+ D KP Sbjct: 228 KWLEEEELHAC--AGSKVVNLADIYES-YQSAKRLYEKKFFVEKGRLFTADQLVDRSKPE 284 Query: 298 NFEIPTEEI-------VKIGNMLGTGREQEMQALLSQVLDYRKMLLYDISYMEEISTAIN 350 E E + IGNM G ++ + + + +++ + +EI A+ Sbjct: 285 APEFSLESYADRLYYSIDIGNMEGC---------VAIIEELKNVVIVHLQEEKEIKVALF 335 Query: 351 EL---IFDKAFNVYGKEAVEIIKVYRQIGYLYNSPHFQDYYHGVENLLNRMNDFIKGIKS 407 +L I + + + + G +Y H G+ L + ++ Sbjct: 336 QLYSNILHQLVRSHPNMEGVLEPLVMNAGDIYQFEHLP----GIMAFLTKQLKYVIDCMD 391 Query: 408 AHLNQREMKAALLFIEQNYNRSDFNLAMVSNHVSFNYSYFSSAFKEYMGMSFSQYIKKLR 467 ++ + I ++Y + + L +++ ++N +Y FK Y G F+ Y+ K+R Sbjct: 392 NGQPDLLLRKMIALIHKDYAK-NLRLETLASVFNYNRAYLGKLFKNYTGEYFNTYLDKVR 450 Query: 468 LVKAKELLENSAYKVYEVSAKVGFENPKHFNKVFREAEGISPLEYR 513 + K LL+ YKVYEV+ K+G+ N +F++ F++ G+SP +R Sbjct: 451 IENGKRLLQ-KGYKVYEVAEKIGYANVDYFHRKFKKYVGVSPSTFR 495 >ref|NP_801837.1| putative two-component response regulator [Streptococcus pyogenes SSI-1] dbj|BAC63670.1| putative two-component response regulator [Streptococcus pyogenes SSI-1] Length = 495 Score = 110 bits (274), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 130/555 (23%), Positives = 229/555 (41%), Gaps = 116/555 (20%) Query: 1 MTRNLLIVDDEENIRLGIRAMLEREYPGMYRYFFAEDGEEALQQLNQAAFDIMMTDIRMP 60 M +L+VDDE I G+ +++ + G A G+EAL L Q D+M++D+ MP Sbjct: 1 MMYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYLTQYPVDVMISDVTMP 60 Query: 61 VMDGITLIGRVQQLEHRPVVVIISGYDDFEYAKQAIRFGVRDYLLKPIVRSELRGTFSRL 120 M G+ LI + + +I+SGY +F Y ++A+ + YLLKP+ ++EL+ + Sbjct: 61 GMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPVDKAELQAKMKQF 120 Query: 121 EQALKREQDINGMLSKATQREQDYRQNEMYYILMNIEIAGPELSERLDRAGLEDLQAGYV 180 + L Q+ + RQ + L+ + + ELSE+ + + L A + Sbjct: 121 KDCLD------------AQQAESIRQEAYHDSLLTLWLTD-ELSEKEFQQLSQGLPAAAL 167 Query: 181 IGLIQEAEANGRTEGGGVLYSRIHEFLHTEALAEEYHFLDKEGRSLVVIRDEQRLGKLLE 240 G VLY E+ +A + Y ++EG+ + ++E + L Sbjct: 168 TGFT-------------VLYLDCQEW---QAAIDAY--FEREGQPFYLKKEEDKKVYLAV 209 Query: 241 YLGQ-DRLLAC-------------QIGSSERMDKAEQFKTAYAQALKALRYFFLKSSTGL 286 LG+ D+ A Q+ E +D E +Y Q LK+L F Sbjct: 210 LLGKTDQAKAFTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSL---FYNCEVSP 266 Query: 287 IRYSQIKDKPRNFEIPTE--EIVKIGNMLGTGREQEMQALLSQVLDYRKMLLYDISYMEE 344 IR KDK + E+P + L G E + + L + D K L ++ E Sbjct: 267 IR---AKDKSK-IELPESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTL----NFSPE 318 Query: 345 ISTAINELIFDKAFNVYGKEAVEIIKVYRQIGYLYNSPHFQDYYHGVENLLNRMNDFIKG 404 ++ L+F +YRQ +L++M ++ Sbjct: 319 DVKHVSFLLFS--------------DIYRQFP-----------------ILDKMT-YLSM 346 Query: 405 IKSAHLNQ------REMKAALLFIEQNYN--------------------RSDFNLAMVSN 438 +K+ H +Q RE+K L QN + + L +++ Sbjct: 347 VKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRKEYHQELTLKAIAD 406 Query: 439 HVSFNYSYFSSAFKEYMGMSFSQYIKKLRLVKAKELLENSAYKVYEVSAKVGFENPKHFN 498 + N Y FK SF+QY+ +R+ KA++LL + + E++ + G+ +F Sbjct: 407 RLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSINEIAYETGYNTNHYFI 466 Query: 499 KVFREAEGISPLEYR 513 K+F++ G+SP E+R Sbjct: 467 KMFKKLNGLSPKEFR 481 >ref|NP_607656.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS8232] gb|AAL98155.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS8232] Length = 494 Score = 109 bits (272), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 129/551 (23%), Positives = 228/551 (41%), Gaps = 116/551 (21%) Query: 5 LLIVDDEENIRLGIRAMLEREYPGMYRYFFAEDGEEALQQLNQAAFDIMMTDIRMPVMDG 64 +L+VDDE I G+ +++ + G A G+EAL L Q D+M++D+ MP M G Sbjct: 4 VLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALTYLTQYPVDVMISDVTMPGMTG 63 Query: 65 ITLIGRVQQLEHRPVVVIISGYDDFEYAKQAIRFGVRDYLLKPIVRSELRGTFSRLEQAL 124 + LI + + +I+SGY +F Y ++A+ + YLLKP+ ++EL+ + + L Sbjct: 64 LDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPVDKAELQAKMKQFKDCL 123 Query: 125 KREQDINGMLSKATQREQDYRQNEMYYILMNIEIAGPELSERLDRAGLEDLQAGYVIGLI 184 Q+ + RQ + L+ + + ELSE+ + + L A + G Sbjct: 124 D------------AQQAESIRQEAYHDSLLTLWLTD-ELSEKEFQQLSQGLPAAALTGFT 170 Query: 185 QEAEANGRTEGGGVLYSRIHEFLHTEALAEEYHFLDKEGRSLVVIRDEQRLGKLLEYLGQ 244 VLY E+ +A + Y ++EG+ + ++E + L LG+ Sbjct: 171 -------------VLYLDCQEW---QAAIDAY--FEREGQPFYLKKEEDKKVYLAVLLGK 212 Query: 245 -DRLLAC-------------QIGSSERMDKAEQFKTAYAQALKALRYFFLKSSTGLIRYS 290 D+ A Q+ E +D E +Y Q LK+L F IR Sbjct: 213 TDQAKAFTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSL---FYNCEVSPIR-- 267 Query: 291 QIKDKPRNFEIPTE--EIVKIGNMLGTGREQEMQALLSQVLDYRKMLLYDISYMEEISTA 348 KDK + E+P + L G E + + L + D K L ++ E Sbjct: 268 -AKDKSK-IELPESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTL----NFSPEDVKH 321 Query: 349 INELIFDKAFNVYGKEAVEIIKVYRQIGYLYNSPHFQDYYHGVENLLNRMNDFIKGIKSA 408 ++ L+F +YRQ +L++M ++ +K+ Sbjct: 322 VSFLLFS--------------DIYRQFP-----------------ILDKMT-YLSMVKTI 349 Query: 409 HLNQ------REMKAALLFIEQNYN--------------------RSDFNLAMVSNHVSF 442 H +Q RE+K L QN + + L +++ + Sbjct: 350 HDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRKEYHQELTLKAIADRLHV 409 Query: 443 NYSYFSSAFKEYMGMSFSQYIKKLRLVKAKELLENSAYKVYEVSAKVGFENPKHFNKVFR 502 N Y FK SF+QY+ +R+ KA++LL + + E++ + G+ +F K+F+ Sbjct: 410 NGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSINEIAYETGYNTNHYFIKMFK 469 Query: 503 EAEGISPLEYR 513 + G+SP E+R Sbjct: 470 KLNGLSPKEFR 480 >ref|NP_665090.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS315] gb|AAM79893.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS315] Length = 494 Score = 109 bits (272), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 129/551 (23%), Positives = 228/551 (41%), Gaps = 116/551 (21%) Query: 5 LLIVDDEENIRLGIRAMLEREYPGMYRYFFAEDGEEALQQLNQAAFDIMMTDIRMPVMDG 64 +L+VDDE I G+ +++ + G A G+EAL L Q D+M++D+ MP M G Sbjct: 4 VLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYLTQYPVDVMISDVTMPGMTG 63 Query: 65 ITLIGRVQQLEHRPVVVIISGYDDFEYAKQAIRFGVRDYLLKPIVRSELRGTFSRLEQAL 124 + LI + + +I+SGY +F Y ++A+ + YLLKP+ ++EL+ + + L Sbjct: 64 LDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPVDKAELQAKMKQFKDCL 123 Query: 125 KREQDINGMLSKATQREQDYRQNEMYYILMNIEIAGPELSERLDRAGLEDLQAGYVIGLI 184 Q+ + RQ + L+ + + ELSE+ + + L A + G Sbjct: 124 D------------AQQAESIRQEAYHDSLLTLWLTD-ELSEKEFQQLSQGLPAAALTGFT 170 Query: 185 QEAEANGRTEGGGVLYSRIHEFLHTEALAEEYHFLDKEGRSLVVIRDEQRLGKLLEYLGQ 244 VLY E+ +A + Y ++EG+ + ++E + L LG+ Sbjct: 171 -------------VLYLDCQEW---QAAIDAY--FEREGQPFYLKKEEDKKVYLAVLLGK 212 Query: 245 -DRLLAC-------------QIGSSERMDKAEQFKTAYAQALKALRYFFLKSSTGLIRYS 290 D+ A Q+ E +D E +Y Q LK+L F IR Sbjct: 213 TDQAKAFTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSL---FYNCEVSPIR-- 267 Query: 291 QIKDKPRNFEIPTE--EIVKIGNMLGTGREQEMQALLSQVLDYRKMLLYDISYMEEISTA 348 KDK + E+P + L G E + + L + D K L ++ E Sbjct: 268 -AKDKSK-IELPESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTL----NFSPEDVKH 321 Query: 349 INELIFDKAFNVYGKEAVEIIKVYRQIGYLYNSPHFQDYYHGVENLLNRMNDFIKGIKSA 408 ++ L+F +YRQ +L++M ++ +K+ Sbjct: 322 VSFLLFS--------------DIYRQFP-----------------ILDKMT-YLSMVKTI 349 Query: 409 HLNQ------REMKAALLFIEQNYN--------------------RSDFNLAMVSNHVSF 442 H +Q RE+K L QN + + L +++ + Sbjct: 350 HDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRKEYHQELTLKAIADRLHV 409 Query: 443 NYSYFSSAFKEYMGMSFSQYIKKLRLVKAKELLENSAYKVYEVSAKVGFENPKHFNKVFR 502 N Y FK SF+QY+ +R+ KA++LL + + E++ + G+ +F K+F+ Sbjct: 410 NGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSINEIAYETGYNTNHYFIKMFK 469 Query: 503 EAEGISPLEYR 513 + G+SP E+R Sbjct: 470 KLNGLSPKEFR 480 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 734,875,410 Number of extensions: 30156697 Number of successful extensions: 99869 Number of sequences better than 1.0e-05: 1639 Number of HSP's gapped: 102234 Number of HSP's successfully gapped: 1677 Length of query: 534 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 404 Effective length of database: 404,274,708 Effective search space: 163326982032 Effective search space used: 163326982032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)