BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0126.0008.p (158 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_616768.1| glyoxalase [Methanosarcina acetivorans str. C2A... 116 1e-25 ref|ZP_00118257.1| COG3324: Predicted enzyme related to lactoylg... 113 1e-24 ref|NP_616934.1| glyoxalase [Methanosarcina acetivorans str. C2A... 78 7e-14 ref|ZP_00148849.1| hypothetical protein Mbur054501 [Methanococco... 71 8e-12 ref|NP_632256.1| hypothetical protein [Methanosarcina mazei Goe1... 69 3e-11 >ref|NP_616768.1| glyoxalase [Methanosarcina acetivorans str. C2A] gb|AAM05248.1| glyoxalase [Methanosarcina acetivorans str. C2A] Length = 129 Score = 116 bits (291), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 60/131 (45%), Positives = 76/131 (58%), Gaps = 2/131 (1%) Query: 28 MGRLVHFEIHVDDMERAKKFYGEVFGWTFEDWSEYAGMPYFGATTGDAGELGINGALMQR 87 M R++HFEI +D+ERAKKFY +VFGW E W M Y+ TTG E GING +M+R Sbjct: 1 MPRVIHFEIRAEDIERAKKFYEDVFGWKIEKWE--GPMEYWSITTGKQEEPGINGGMMRR 58 Query: 88 RGASPEPGQAMNGYACTMGVEDYDSTEAIILKLGGKLALPKYALPGMAWQGYYLDTEGNT 147 + P ++ Y CT+ V D D I K GGK+ K + G+ W Y LDTE N Sbjct: 59 QVGEPGADTPISTYICTIDVPDIDKYLNQIQKHGGKVTGEKRPITGVGWLAYCLDTEKNI 118 Query: 148 FGIHQPDKHAK 158 FGI QPD A+ Sbjct: 119 FGIMQPDMSAR 129 >ref|ZP_00118257.1| COG3324: Predicted enzyme related to lactoylglutathione lyase [Cytophaga hutchinsonii] Length = 128 Score = 113 bits (283), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 57/131 (43%), Positives = 74/131 (56%), Gaps = 6/131 (4%) Query: 28 MGRLVHFEIHVDDMERAKKFYGEVFGWTFEDWSEYAGMPYFGATTGDAGELGINGALMQR 87 M + FEI D R +FY VFGW F E+ + Y+ T I G L++R Sbjct: 4 MNTIGFFEIQSSDPARDIQFYKTVFGWKFTK-EEFVPIEYYRIDTE-----SIYGGLLKR 57 Query: 88 RGASPEPGQAMNGYACTMGVEDYDSTEAIILKLGGKLALPKYALPGMAWQGYYLDTEGNT 147 A P G N + C+M VED+D T +IL GG++ALPK+A+PG WQGY+LD + NT Sbjct: 58 TAALPLTGTGTNAFTCSMQVEDFDRTAEVILSSGGQVALPKFAIPGRCWQGYFLDPDNNT 117 Query: 148 FGIHQPDKHAK 158 FGI Q D AK Sbjct: 118 FGIFQVDSQAK 128 >ref|NP_616934.1| glyoxalase [Methanosarcina acetivorans str. C2A] gb|AAM05414.1| glyoxalase [Methanosarcina acetivorans str. C2A] Length = 123 Score = 77.8 bits (190), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 49/131 (37%), Positives = 65/131 (49%), Gaps = 8/131 (6%) Query: 28 MGRLVHFEIHVDDMERAKKFYGEVFGWTFEDWSEYAGMPYFGATTGDAGELGINGALMQR 87 M +VHF++ DD RAKKFY E+FGW FE + M Y+ T D L Sbjct: 1 MPTIVHFDVPADDPARAKKFYAELFGWKFE--KPFETMEYYLIETED---LEGKAGPGGG 55 Query: 88 RGASPEPGQAMNGYACTMGVEDYDSTEAIILKLGGKLALPKYALPGMAWQGYYLDTEGNT 147 G P Q + Y +E+Y S + KLGGK+ + K A+PG + DTE NT Sbjct: 56 LGKRGAPDQRIMNYIGVPSIEEYLSK---VEKLGGKVVMAKTAVPGWGYLAICTDTENNT 112 Query: 148 FGIHQPDKHAK 158 FG+ Q +K K Sbjct: 113 FGLWQDEKETK 123 >ref|ZP_00148849.1| hypothetical protein Mbur054501 [Methanococcoides burtonii DSM 6242] Length = 123 Score = 70.9 bits (172), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 45/133 (33%), Positives = 68/133 (51%), Gaps = 14/133 (10%) Query: 28 MGRLVHFEIHVDDMERAKKFYGEVFGWTFEDWS---EYAGMPYFGATTGDAGELGINGAL 84 M + H ++ +D++RAK+FY E+F W FE +Y YF T GE G+ G + Sbjct: 1 MPAIAHLDLPANDIDRAKEFYTELFDWKFEKVPGPMDY----YFIETESLDGEKGVAGGM 56 Query: 85 MQRRGASPEPGQAMNGYACTMGVEDYDSTEAIILKLGGKLALPKYALPGMAWQGYYLDTE 144 RR P Q + + VEDY + + KLGG + PK +PG + +DTE Sbjct: 57 GLRRS----PEQRITNFIEVASVEDYCTR---VEKLGGIVQQPKMPVPGWGYLAVCMDTE 109 Query: 145 GNTFGIHQPDKHA 157 NTFG+ + D++ Sbjct: 110 ENTFGLWEIDENT 122 >ref|NP_632256.1| hypothetical protein [Methanosarcina mazei Goe1] gb|AAM29928.1| hypothetical protein [Methanosarcina mazei Goe1] Length = 123 Score = 69.3 bits (168), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 44/131 (33%), Positives = 65/131 (49%), Gaps = 8/131 (6%) Query: 28 MGRLVHFEIHVDDMERAKKFYGEVFGWTFEDWSEYAGMPYFGATTGDAGELGINGALMQR 87 M +VHF+I DD ERAK FY +FGW FE M Y+ T G +++ Sbjct: 1 MPTIVHFDIPADDSERAKNFYSRLFGWKFE--KPLETMDYYLIETEGPEGESGLGGGLRK 58 Query: 88 RGASPEPGQAMNGYACTMGVEDYDSTEAIILKLGGKLALPKYALPGMAWQGYYLDTEGNT 147 R + Q + Y +GV D + KLGG++ +PK A+PG +DTE N Sbjct: 59 REGA---DQRIMNY---IGVPSVDEYLEKVEKLGGRVLMPKIAIPGWGHLAICMDTENNA 112 Query: 148 FGIHQPDKHAK 158 FG+ Q ++ ++ Sbjct: 113 FGLWQDEEKSE 123 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.139 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 271,014,360 Number of extensions: 11424924 Number of successful extensions: 24317 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 24335 Number of HSP's successfully gapped: 13 Length of query: 158 Length of database: 757,237,578 Length adjustment: 115 Effective length of query: 43 Effective length of database: 445,001,193 Effective search space: 19135051299 Effective search space used: 19135051299 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)