BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0118.0007.p (548 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|P20533|CHI1_BACCI Chitinase A1 precursor >gi|98111|pir||A3836... 653 0.0 pdb|1ITX|A Chain A, Catalytic Domain Of Chitinase A1 From Bacill... 645 0.0 gb|AAF74782.1| chitinase [Bacillus circulans] 643 0.0 dbj|BAA09831.1| chitinase [Kurthia zopfii] 641 0.0 ref|NP_241782.1| chitinase [Bacillus halodurans] >gi|25486807|pi... 572 e-162 >sp|P20533|CHI1_BACCI Chitinase A1 precursor pir||A38368 chitinase (EC 3.2.1.14) precursor - Bacillus circulans gb|AAA81528.1| chitinase A1 Length = 699 Score = 653 bits (1684), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/415 (74%), Positives = 344/415 (82%) Query: 36 TANAAAGYKLVGYYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNP 95 TA AA YK+VGYY SWAAYGR+YNV DIDP K+ INYAFADICWNG+HGNPDP+GPNP Sbjct: 37 TAEAADSYKIVGYYPSWAAYGRNYNVADIDPTKVTHINYAFADICWNGIHGNPDPSGPNP 96 Query: 96 VTWTCQNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLK 155 VTWTCQNE+ QTI+VPNGT+VLGDPWID K+F D WD PI GN+ QL KLK+ NPNLK Sbjct: 97 VTWTCQNEKSQTINVPNGTIVLGDPWIDTGKTFAGDTWDQPIAGNINQLNKLKQTNPNLK 156 Query: 156 TVISIGGWTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNSY 215 T+IS+GGWTWSNRFSDVAATAATREVFANSAVDF+RKY+ DGVDLDWEYPVSGGL GNS Sbjct: 157 TIISVGGWTWSNRFSDVAATAATREVFANSAVDFLRKYNFDGVDLDWEYPVSGGLDGNSK 216 Query: 216 RPEDKQNYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINI 275 RPEDKQNY LLL KIREKL+AAG DGK YLLTIASGAS Y N L+ IAAIVDWINI Sbjct: 217 RPEDKQNYTLLLSKIREKLDAAGAVDGKKYLLTIASGASATYAANTELAKIAAIVDWINI 276 Query: 276 MTYDFNGSWNTTTGHNSPLYYDPAAASSGLTDPENFNIDKAVTSYLANGVPANKLVLGLP 335 MTYDFNG+W + HN+PL YDPAA+++G+ D FN+ +L GVPA KLVLG+P Sbjct: 277 MTYDFNGAWQKISAHNAPLNYDPAASAAGVPDANTFNVAAGAQGHLDAGVPAAKLVLGVP 336 Query: 336 FYGRGWGGAPGTGNGQYQVSAGISSTGTWEKGNYDFWDLEANYINKNGYTRYWNNTAKVP 395 FYGRGW G GNGQYQ G SS GTWE G++DF+DLEANYINKNGYTRYWN+TAKVP Sbjct: 337 FYGRGWDGCAQAGNGQYQTCTGGSSVGTWEAGSFDFYDLEANYINKNGYTRYWNDTAKVP 396 Query: 396 YLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGDRNKTLQTKLNTDL 450 YLYN + + FISYDDAES+GYKT+YIK+KGL GAMFWE SGDRNKTLQ KL DL Sbjct: 397 YLYNASNKRFISYDDAESVGYKTAYIKSKGLGGAMFWELSGDRNKTLQNKLKADL 451 >pdb|1ITX|A Chain A, Catalytic Domain Of Chitinase A1 From Bacillus Circulans Wl- 12 Length = 419 Score = 645 bits (1664), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/415 (74%), Positives = 344/415 (82%) Query: 36 TANAAAGYKLVGYYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNP 95 TA AA YK+VGYY SWAAYGR+YNV DIDP K+ INYAFADICWNG+HGNPDP+GPNP Sbjct: 5 TAEAADSYKIVGYYPSWAAYGRNYNVADIDPTKVTHINYAFADICWNGIHGNPDPSGPNP 64 Query: 96 VTWTCQNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLK 155 VTWTCQNE+ QTI+VPNGT+VLGDPWID K+F D WD PI GN+ QL KLK+ NPNLK Sbjct: 65 VTWTCQNEKSQTINVPNGTIVLGDPWIDTGKTFAGDTWDQPIAGNINQLNKLKQTNPNLK 124 Query: 156 TVISIGGWTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNSY 215 T+IS+GGWTWSNRFSDVAATAATREVFANSAVDF+RKY+ DGVDLDWEYPVSGGL GNS Sbjct: 125 TIISVGGWTWSNRFSDVAATAATREVFANSAVDFLRKYNFDGVDLDWEYPVSGGLDGNSK 184 Query: 216 RPEDKQNYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINI 275 RPEDKQNY LLL KIREKL+AAG DGK YLLTIASGAS Y N L+ IAAIVDWINI Sbjct: 185 RPEDKQNYTLLLSKIREKLDAAGAVDGKKYLLTIASGASATYAANTELAKIAAIVDWINI 244 Query: 276 MTYDFNGSWNTTTGHNSPLYYDPAAASSGLTDPENFNIDKAVTSYLANGVPANKLVLGLP 335 MTYDFNG+W + HN+PL YDPAA+++G+ D FN+ +L GVPA KLVLG+P Sbjct: 245 MTYDFNGAWQKISAHNAPLNYDPAASAAGVPDANTFNVAAGAQGHLDAGVPAAKLVLGVP 304 Query: 336 FYGRGWGGAPGTGNGQYQVSAGISSTGTWEKGNYDFWDLEANYINKNGYTRYWNNTAKVP 395 FYGRGW G GNGQYQ G SS GTWE G++DF+DLEANYINKNGYTRYWN+TAKVP Sbjct: 305 FYGRGWDGCAQAGNGQYQTCTGGSSVGTWEAGSFDFYDLEANYINKNGYTRYWNDTAKVP 364 Query: 396 YLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGDRNKTLQTKLNTDL 450 YLYN + + FISYDDAES+GYKT+YIK+KGL GAMFWE SGDRNKTLQ KL DL Sbjct: 365 YLYNASNKRFISYDDAESVGYKTAYIKSKGLGGAMFWELSGDRNKTLQNKLKADL 419 >gb|AAF74782.1| chitinase [Bacillus circulans] Length = 717 Score = 643 bits (1659), Expect = 0.0, Method: Compositional matrix adjust. Identities = 303/416 (72%), Positives = 344/416 (82%) Query: 35 KTANAAAGYKLVGYYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPN 94 +TA AA YK+VGYY +WAAYGR+YNVTDIDP K+ INYAFADICWNG+HGNPDP+GPN Sbjct: 36 RTAEAADAYKIVGYYPAWAAYGRNYNVTDIDPTKVTHINYAFADICWNGIHGNPDPSGPN 95 Query: 95 PVTWTCQNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNL 154 PVTW+CQNE+ QTI+VPNGT+VLGDPWID K F D WD P GN+ QL KLK+ NPNL Sbjct: 96 PVTWSCQNEKSQTINVPNGTIVLGDPWIDTGKQFAGDTWDQPYAGNINQLNKLKQVNPNL 155 Query: 155 KTVISIGGWTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNS 214 KT+ISIGGWTWSNRFSDVAAT+ATREVFANSAVDF+RKY+ DGVDL+WEYPVSGGL GNS Sbjct: 156 KTIISIGGWTWSNRFSDVAATSATREVFANSAVDFLRKYNFDGVDLNWEYPVSGGLDGNS 215 Query: 215 YRPEDKQNYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWIN 274 RPEDKQNY LLL KIREKL+AA DGK YLLTIASGASP Y N L+ IA+IVDWIN Sbjct: 216 KRPEDKQNYTLLLSKIREKLDAAEAVDGKEYLLTIASGASPTYAANTELANIASIVDWIN 275 Query: 275 IMTYDFNGSWNTTTGHNSPLYYDPAAASSGLTDPENFNIDKAVTSYLANGVPANKLVLGL 334 IMTYDFNG+W + HN+PL DPAAAS+G+ D FN+ +L GVPA KLVLG+ Sbjct: 276 IMTYDFNGAWQKISAHNAPLNADPAAASAGVPDSNTFNVAAGAQGHLNAGVPAAKLVLGV 335 Query: 335 PFYGRGWGGAPGTGNGQYQVSAGISSTGTWEKGNYDFWDLEANYINKNGYTRYWNNTAKV 394 PFYGRGW G NGQYQ +G SS GTWE G++DF+DLEANYINKNGYTRYWN+TAKV Sbjct: 336 PFYGRGWDGCAQANNGQYQTCSGGSSIGTWEAGSFDFYDLEANYINKNGYTRYWNDTAKV 395 Query: 395 PYLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGDRNKTLQTKLNTDL 450 P+LYN + + FISYDDAESIG+KT+YIK+KGL GAMFWE SGDRNKTLQ KL +DL Sbjct: 396 PFLYNASNKRFISYDDAESIGHKTAYIKSKGLGGAMFWELSGDRNKTLQNKLKSDL 451 >dbj|BAA09831.1| chitinase [Kurthia zopfii] Length = 699 Score = 641 bits (1654), Expect = 0.0, Method: Compositional matrix adjust. Identities = 305/415 (73%), Positives = 341/415 (82%) Query: 36 TANAAAGYKLVGYYASWAAYGRSYNVTDIDPGKMNVINYAFADICWNGVHGNPDPTGPNP 95 TA+AA YK+VGYY SWA YGR+YNVTDIDP K+ INYAFADICWNGVHGNPDP+GPNP Sbjct: 37 TASAADAYKIVGYYPSWAGYGRNYNVTDIDPTKVTHINYAFADICWNGVHGNPDPSGPNP 96 Query: 96 VTWTCQNEQGQTISVPNGTVVLGDPWIDAQKSFGDDKWDDPIKGNLKQLWKLKEKNPNLK 155 VTWTCQNE+ QTI+VPNGT+VLGDPWID K+F D WD PI GN+ QL KLK+ NPNLK Sbjct: 97 VTWTCQNEKSQTINVPNGTIVLGDPWIDTGKTFAGDTWDQPIAGNINQLNKLKQINPNLK 156 Query: 156 TVISIGGWTWSNRFSDVAATAATREVFANSAVDFIRKYHMDGVDLDWEYPVSGGLAGNSY 215 T+IS+GGWTWSNRFSDVAATAATREVFANSAVDF+RKY+ DGVDLDWEYPVSGGL GNS Sbjct: 157 TIISVGGWTWSNRFSDVAATAATREVFANSAVDFLRKYNFDGVDLDWEYPVSGGLDGNSK 216 Query: 216 RPEDKQNYVLLLQKIREKLNAAGTADGKTYLLTIASGASPAYVQNNNLSGIAAIVDWINI 275 R DKQNY LLL KIREKL+AAG DGK YLLTIASGAS Y N L+ IA+IVDWINI Sbjct: 217 RSGDKQNYTLLLSKIREKLDAAGAVDGKKYLLTIASGASTTYAANTELANIASIVDWINI 276 Query: 276 MTYDFNGSWNTTTGHNSPLYYDPAAASSGLTDPENFNIDKAVTSYLANGVPANKLVLGLP 335 MTYDFNG+W + HN+ L YDPAA+++G+ D FN+ +L GVPA KLVLG+P Sbjct: 277 MTYDFNGAWQKVSAHNALLNYDPAASAAGVPDANTFNVAAGAQGHLNAGVPAAKLVLGVP 336 Query: 336 FYGRGWGGAPGTGNGQYQVSAGISSTGTWEKGNYDFWDLEANYINKNGYTRYWNNTAKVP 395 FYGRGW G GNGQYQ G SS GTWE G++DF+DLE NYINKNGYTRYWN+TAKVP Sbjct: 337 FYGRGWDGCAQAGNGQYQTCTGGSSVGTWEAGSFDFYDLETNYINKNGYTRYWNDTAKVP 396 Query: 396 YLYNPTTQTFISYDDAESIGYKTSYIKTKGLAGAMFWETSGDRNKTLQTKLNTDL 450 YLYN + + FISYDDAESIGYKT+YIK+KGL GAMFWE SGDRNKTLQ KL DL Sbjct: 397 YLYNASNKRFISYDDAESIGYKTAYIKSKGLGGAMFWELSGDRNKTLQNKLKADL 451 >ref|NP_241782.1| chitinase [Bacillus halodurans] pir||D83764 chitinase BH0916 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04635.1| chitinase [Bacillus halodurans] Length = 599 Score = 572 bits (1475), Expect = e-162, Method: Compositional matrix adjust. Identities = 279/446 (62%), Positives = 333/446 (74%), Gaps = 6/446 (1%) Query: 9 RFRKTFALGMAVALVIALFSTIGTSVKTANAAA----GYKLVGYYASWAAYGRSYNVTDI 64 RFRK + + + L T + + AA YK+V YY SW AYGR Y V DI Sbjct: 2 RFRKRMGKLLIWIVTVLLVVTWMPADQVVGAAETSDDQYKIVAYYPSWGAYGRDYQVWDI 61 Query: 65 DPGKMNVINYAFADICWNGVHGNPDPTGPNPVTWTCQNEQGQTISVPNGTVVLGDPWIDA 124 D K++ INYAFA+ICW+G HGNPDP GPNP TW+CQ+E G I VPNG++V+GDPWIDA Sbjct: 62 DASKISHINYAFANICWDGRHGNPDPAGPNPQTWSCQDENG-VIDVPNGSIVMGDPWIDA 120 Query: 125 QKSFGDDKWDDPIKGNLKQLWKLKEKNPNLKTVISIGGWTWSNRFSDVAATAATREVFAN 184 QKS D WD+P++GN KQL KLKE++P+LKT+IS+GGWTWSNRFSD+AAT TRE FAN Sbjct: 121 QKSNPGDTWDEPLRGNFKQLNKLKEEHPHLKTLISVGGWTWSNRFSDMAATKETRENFAN 180 Query: 185 SAVDFIRKYHMDGVDLDWEYPVSGGLAGNSYRPEDKQNYVLLLQKIREKLNAAGTADGKT 244 SAV+FIRKY DGVD+DWEYPVSGGL GNS RPEDK+N+VLLLQ++R+KL+ AG DGK Sbjct: 181 SAVEFIRKYGFDGVDVDWEYPVSGGLPGNSRRPEDKENHVLLLQEVRDKLDEAGQEDGKD 240 Query: 245 YLLTIASGASPAYVQNNNLSGIAAIVDWINIMTYDFNGSWNTTTGHNSPLYYDPAAASSG 304 YLLTIASGASP YV+NN L+ IA IVDWINIMTYDFNG W +GHN+PLYYDPA A++ Sbjct: 241 YLLTIASGASPGYVENNKLNEIAEIVDWINIMTYDFNGGWQNISGHNAPLYYDPATANTE 300 Query: 305 LTDPENFNIDKAVTSYLANGVPANKLVLGLPFYGRGWGGAPGTGNGQYQVSAGISSTGTW 364 L PE+FN++ AV +L GVP +KLVLG+PFYGRGW G G+YQ A GTW Sbjct: 301 LPTPEHFNVESAVEGHLQAGVPEHKLVLGMPFYGRGWSNCDGANQGEYQRCA-PPREGTW 359 Query: 365 EKGNYDFWDLEANYINKNGYTRYWNNTAKVPYLYNPTTQTFISYDDAESIGYKTSYIKTK 424 E G +DF DLE +YINKNGY RYWN+ AKVP+LYN T FI+YDD ES YKT +IK+ Sbjct: 360 ENGVFDFSDLEDHYINKNGYQRYWNDVAKVPFLYNATNGNFITYDDEESFRYKTDFIKSN 419 Query: 425 GLAGAMFWETSGDRNKTLQTKLNTDL 450 LAG+MFW+ SGDRN TL T L L Sbjct: 420 NLAGSMFWDVSGDRNGTLLTALADQL 445 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 850,986,949 Number of extensions: 39475782 Number of successful extensions: 107405 Number of sequences better than 1.0e-05: 748 Number of HSP's gapped: 104750 Number of HSP's successfully gapped: 789 Length of query: 548 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 417 Effective length of database: 401,559,609 Effective search space: 167450356953 Effective search space used: 167450356953 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)