BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0112.0006.p (333 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAA12151.1| ORF1 [Bacillus megaterium] 482 e-135 ref|NP_390416.1| yqfA [Bacillus subtilis] >gi|1730994|sp|P54466|... 480 e-134 ref|ZP_00061648.1| COG4864: Uncharacterized protein conserved in... 479 e-134 ref|NP_692880.1| hypothetical protein OB1959 [Oceanobacillus ihe... 473 e-132 ref|ZP_00103976.1| COG4864: Uncharacterized protein conserved in... 468 e-131 >emb|CAA12151.1| ORF1 [Bacillus megaterium] Length = 327 Score = 482 bits (1241), Expect = e-135, Method: Compositional matrix adjust. Identities = 237/299 (79%), Positives = 267/299 (89%) Query: 28 PVMLWVSAWAAGVRISIITLVAMRLRRVTPSRIVNPLIKATKAGLGVNINQLESHYLAGG 87 P+MLW+SA AAGVRISI TLV MRLRRV PSR+VNPLIKA+KAGLG+ INQLESHYLAGG Sbjct: 27 PIMLWISALAAGVRISIFTLVGMRLRRVIPSRVVNPLIKASKAGLGITINQLESHYLAGG 86 Query: 88 NVDRVVNALIAAQRADIPLEFTRAAAIDLAGRDVLLAVQMSVNPRVIETPIVAAVAKNGI 147 NVDRVVNALIAA RA+I L F R AAIDLAGRDVL AVQMSVNP+VIETP +A VA +GI Sbjct: 87 NVDRVVNALIAAHRANIELTFERGAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGI 146 Query: 148 EVKVKARVTVRANIDRLVGGAGEETIIARVGEGIVTTVGSSSTHKDVLENPDSISRTVLS 207 EVK KAR+TVRANIDRLVGGAGEETIIARVGEGIV+T+GS + HK VLENPD IS+TVL Sbjct: 147 EVKAKARITVRANIDRLVGGAGEETIIARVGEGIVSTIGSQTDHKKVLENPDMISQTVLG 206 Query: 208 KGLDSGTAFEILSIDIADVDVGKNIGAYLQTEQAEADKRIAQAKAEERRAMAVAHEQEMK 267 KGLDSGTAFEILSIDIAD+D+GKNIGA LQT+QAE DK IAQAKAEERRAMAVA EQEM+ Sbjct: 207 KGLDSGTAFEILSIDIADIDIGKNIGAVLQTDQAERDKNIAQAKAEERRAMAVAQEQEMR 266 Query: 268 ARVVEMKALVVESESQVPLAMAEALRSGKLGVMDYMNLKNIEADTQMRGSLGKLNDGDD 326 A+V EM+A VVE+E++VPLAMAEALRSG +GVMDYMN++N+ ADT MR S+GK++ DD Sbjct: 267 AKVEEMRAKVVEAEAEVPLAMAEALRSGNIGVMDYMNIQNLTADTDMRDSIGKMSKEDD 325 >ref|NP_390416.1| yqfA [Bacillus subtilis] sp|P54466|YQFA_BACSU Hypothetical protein yqfA pir||A69953 hypothetical protein yqfA - Bacillus subtilis dbj|BAA12473.1| YqfA [Bacillus subtilis] emb|CAB14480.1| yqfA [Bacillus subtilis subsp. subtilis str. 168] Length = 331 Score = 480 bits (1236), Expect = e-134, Method: Compositional matrix adjust. Identities = 241/305 (79%), Positives = 267/305 (87%), Gaps = 1/305 (0%) Query: 28 PVMLWVSAWAAGVRISIITLVAMRLRRVTPSRIVNPLIKATKAGLGVNINQLESHYLAGG 87 PVMLW+SA AAGV+ISI TLV MRLRRV P+R+VNPLIKA KAGL V NQLESHYLAGG Sbjct: 27 PVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGG 86 Query: 88 NVDRVVNALIAAQRADIPLEFTRAAAIDLAGRDVLLAVQMSVNPRVIETPIVAAVAKNGI 147 NVDRVVNALIAAQRA+I L F R AAIDLAGRDVL AVQMSVNP+VIETP +A VA +GI Sbjct: 87 NVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGI 146 Query: 148 EVKVKARVTVRANIDRLVGGAGEETIIARVGEGIVTTVGSSSTHKDVLENPDSISRTVLS 207 EVK KAR+TVRANI+RLVGGAGEETI+ARVGEGIV+T+GSS HK VLENPD IS+TVL Sbjct: 147 EVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLG 206 Query: 208 KGLDSGTAFEILSIDIADVDVGKNIGAYLQTEQAEADKRIAQAKAEERRAMAVAHEQEMK 267 KGLDSGTAFEILSIDIADVD+GKNIGA LQT+QAEADK IAQAKAEERRAMAVA EQEM+ Sbjct: 207 KGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMR 266 Query: 268 ARVVEMKALVVESESQVPLAMAEALRSGKLGVMDYMNLKNIEADTQMRGSLGKLN-DGDD 326 ARV EM+A VVE+E++VPLAMAEALR G +GVMDYMN+KNI+ADT+MR S GKL D D Sbjct: 267 ARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSD 326 Query: 327 SSRPT 331 R + Sbjct: 327 EDRKS 331 >ref|ZP_00061648.1| COG4864: Uncharacterized protein conserved in bacteria [Clostridium thermocellum ATCC 27405] Length = 329 Score = 479 bits (1234), Expect = e-134, Method: Compositional matrix adjust. Identities = 234/301 (77%), Positives = 276/301 (91%) Query: 28 PVMLWVSAWAAGVRISIITLVAMRLRRVTPSRIVNPLIKATKAGLGVNINQLESHYLAGG 87 PV LW+SA+AA VR+SI TL+ MRLRRV PSR++NPLIKATKAG+ V+IN+LE+HYLAGG Sbjct: 25 PVGLWISAFAANVRVSIFTLIGMRLRRVVPSRVINPLIKATKAGINVSINKLEAHYLAGG 84 Query: 88 NVDRVVNALIAAQRADIPLEFTRAAAIDLAGRDVLLAVQMSVNPRVIETPIVAAVAKNGI 147 NVDRVVNALIAAQRA+IPLEF RAAAIDLAGR+VL AVQMSVNP+VIETP+VAA+AK+GI Sbjct: 85 NVDRVVNALIAAQRANIPLEFERAAAIDLAGRNVLEAVQMSVNPKVIETPVVAAIAKDGI 144 Query: 148 EVKVKARVTVRANIDRLVGGAGEETIIARVGEGIVTTVGSSSTHKDVLENPDSISRTVLS 207 E++ KARVTVRANIDRLVGGAGE+TIIARVGEG+VTTVGS++ HK VLENPD+IS+TVLS Sbjct: 145 ELRAKARVTVRANIDRLVGGAGEQTIIARVGEGVVTTVGSATDHKQVLENPDAISKTVLS 204 Query: 208 KGLDSGTAFEILSIDIADVDVGKNIGAYLQTEQAEADKRIAQAKAEERRAMAVAHEQEMK 267 KGLD+GTAFEILSIDIAD+DVG+N+GA LQT+QAEADKRIAQAKAEERRAMAVA EQEMK Sbjct: 205 KGLDAGTAFEILSIDIADIDVGRNVGAQLQTDQAEADKRIAQAKAEERRAMAVAREQEMK 264 Query: 268 ARVVEMKALVVESESQVPLAMAEALRSGKLGVMDYMNLKNIEADTQMRGSLGKLNDGDDS 327 A V EM+A VVE+E++VP A+A ALR GK+GV+DY +L+N+ ADTQMR S+ K++ DDS Sbjct: 265 AMVQEMRAKVVEAEAEVPKALAAALREGKIGVLDYYHLQNLIADTQMRDSISKMSKHDDS 324 Query: 328 S 328 S Sbjct: 325 S 325 >ref|NP_692880.1| hypothetical protein OB1959 [Oceanobacillus iheyensis HTE831] dbj|BAC13915.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 330 Score = 473 bits (1218), Expect = e-132, Method: Compositional matrix adjust. Identities = 238/302 (78%), Positives = 264/302 (87%), Gaps = 3/302 (0%) Query: 28 PVMLWVSAWAAGVRISIITLVAMRLRRVTPSRIVNPLIKATKAGLGVNINQLESHYLAGG 87 PV LW+SA AAGV+ISI TL+ MRLRRV P+R++NPLIKA KAGL V NQLESHYLAGG Sbjct: 27 PVALWISALAAGVKISIFTLIGMRLRRVVPNRVINPLIKAHKAGLNVKTNQLESHYLAGG 86 Query: 88 NVDRVVNALIAAQRADIPLEFTRAAAIDLAGRDVLLAVQMSVNPRVIETPIVAAVAKNGI 147 NVDRVVNALIAA RA+I L F R AAIDLAGRDVL AVQMSVNP+VIETP +A +A +GI Sbjct: 87 NVDRVVNALIAAHRANIDLPFERGAAIDLAGRDVLEAVQMSVNPKVIETPFIAGIAMDGI 146 Query: 148 EVKVKARVTVRANIDRLVGGAGEETIIARVGEGIVTTVGSSSTHKDVLENPDSISRTVLS 207 EVK AR+TVRANIDRLVGGAGEETIIARVGEG+V+T+GSS HK VLENPDSIS+TVLS Sbjct: 147 EVKALARITVRANIDRLVGGAGEETIIARVGEGVVSTIGSSDHHKQVLENPDSISQTVLS 206 Query: 208 KGLDSGTAFEILSIDIADVDVGKNIGAYLQTEQAEADKRIAQAKAEERRAMAVAHEQEMK 267 KGLDSGTAFEILSIDIADVD+GKNIGA LQT+QAEADK IAQAKAEERRAMAVA EQEM Sbjct: 207 KGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMV 266 Query: 268 ARVVEMKALVVESESQVPLAMAEALRSGKLGVMDYMNLKNIEADTQMRGSLGKL---NDG 324 ARV EM+A VVE+E+ VPLA+AEALRSGK+GVMDYMN +NI+ADT MR S+GKL N Sbjct: 267 ARVQEMRAKVVEAEADVPLALAEALRSGKMGVMDYMNYQNIDADTDMRDSIGKLSKENKD 326 Query: 325 DD 326 DD Sbjct: 327 DD 328 >ref|ZP_00103976.1| COG4864: Uncharacterized protein conserved in bacteria [Desulfitobacterium hafniense] Length = 333 Score = 468 bits (1205), Expect = e-131, Method: Compositional matrix adjust. Identities = 237/303 (78%), Positives = 267/303 (88%) Query: 28 PVMLWVSAWAAGVRISIITLVAMRLRRVTPSRIVNPLIKATKAGLGVNINQLESHYLAGG 87 PV LW+SA AAGV + I TLV MRLRRVTPSRIVNPLIKA KAGL V QLE+HYLAGG Sbjct: 29 PVGLWISALAAGVNVGIFTLVGMRLRRVTPSRIVNPLIKAHKAGLRVTTAQLEAHYLAGG 88 Query: 88 NVDRVVNALIAAQRADIPLEFTRAAAIDLAGRDVLLAVQMSVNPRVIETPIVAAVAKNGI 147 NVDRVVNALIAA+RA IPL+F RAAAIDLAGRDVL AVQMSVNP+VIETP+V+AVAKNGI Sbjct: 89 NVDRVVNALIAAERAAIPLQFERAAAIDLAGRDVLEAVQMSVNPKVIETPVVSAVAKNGI 148 Query: 148 EVKVKARVTVRANIDRLVGGAGEETIIARVGEGIVTTVGSSSTHKDVLENPDSISRTVLS 207 E++VKARVTVRANIDRLVGGAGEETIIARVGEGIVT++GSS +H+ VLENPD +SRTVL+ Sbjct: 149 ELRVKARVTVRANIDRLVGGAGEETIIARVGEGIVTSIGSSLSHEKVLENPDMVSRTVLA 208 Query: 208 KGLDSGTAFEILSIDIADVDVGKNIGAYLQTEQAEADKRIAQAKAEERRAMAVAHEQEMK 267 KGLDSGTAFEILSIDIADVDVGKNIGA LQT+QAEADKRIAQAKAEERRAMAVA EQEM Sbjct: 209 KGLDSGTAFEILSIDIADVDVGKNIGAQLQTDQAEADKRIAQAKAEERRAMAVAKEQEMI 268 Query: 268 ARVVEMKALVVESESQVPLAMAEALRSGKLGVMDYMNLKNIEADTQMRGSLGKLNDGDDS 327 A V EM+A VVE+ES+VP A+AEAL+ GKLGVMDY ++NI ADT MR ++ + ++ + Sbjct: 269 AYVQEMRAKVVEAESEVPRALAEALKEGKLGVMDYYTMQNIMADTSMRDNIARSSNSNTD 328 Query: 328 SRP 330 S P Sbjct: 329 SNP 331 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.130 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 383,683,046 Number of extensions: 13991284 Number of successful extensions: 51132 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 51025 Number of HSP's successfully gapped: 51 Length of query: 333 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 207 Effective length of database: 415,135,104 Effective search space: 85932966528 Effective search space used: 85932966528 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)