BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0112.0003.p
         (119 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF 
           2,715,099 sequences; 757,237,578 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|ZP_00104000.1| COG0537: Diadenosine tetraphosphate (Ap4A) hy...   113   1e-24
ref|NP_347918.1| HIT family hydrolase [Clostridium acetobutylicu...   108   3e-23
ref|NP_213096.1| protein kinase C inhibitor (HIT family) [Aquife...   104   6e-22
gb|EAI77744.1| unknown [environmental sequence]                       103   2e-21
gb|EAI94140.1| unknown [environmental sequence]                       102   3e-21

>ref|ZP_00104000.1| COG0537: Diadenosine tetraphosphate (Ap4A) hydrolase and other
          HIT family hydrolases [Desulfitobacterium hafniense]
          Length = 111

 Score =  113 bits (282), Expect = 1e-24,   Method: Compositional matrix adjust.
 Identities = 54/94 (57%), Positives = 68/94 (72%)

Query: 2  ETVFSKIIEGSIPSKKVFENERILAFYDIEPAAPVHVLIIPKKFIASMNDVAPEDLPLIG 61
          E +F KII   IPS+ VFE+E +LAF DI P APVH+L+IPKK   S+ND+   D  L+G
Sbjct: 3  ECIFCKIINKEIPSQVVFEDEHVLAFKDINPVAPVHLLVIPKKHRESLNDIDVADEALLG 62

Query: 62 EIHAVAQQIAVELGIADSGYRLINNCGPDSGQAV 95
           I  VA+++A E GIADSGYR++NNCG D GQ V
Sbjct: 63 HILVVAKKLAQESGIADSGYRVVNNCGDDGGQLV 96


>ref|NP_347918.1| HIT family hydrolase [Clostridium acetobutylicum]
 pir||G97058 HIT family hydrolase [imported] - Clostridium acetobutylicum
 gb|AAK79258.1| HIT family hydrolase [Clostridium acetobutylicum]
          Length = 114

 Score =  108 bits (271), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 50/94 (53%), Positives = 70/94 (74%)

Query: 2  ETVFSKIIEGSIPSKKVFENERILAFYDIEPAAPVHVLIIPKKFIASMNDVAPEDLPLIG 61
          E +F KII+G IPS KV+E+E +LAF DI PAAPVHVL+IPKK I+S+ND+  E+  +I 
Sbjct: 3  ECIFCKIIKGEIPSSKVYEDEDVLAFNDISPAAPVHVLVIPKKHISSLNDINEENSKVIA 62

Query: 62 EIHAVAQQIAVELGIADSGYRLINNCGPDSGQAV 95
           +  V  ++A ELGI + G+R+++NCG  +GQ V
Sbjct: 63 HVFVVISKLAKELGIDEDGFRVVSNCGEAAGQTV 96


>ref|NP_213096.1| protein kinase C inhibitor (HIT family) [Aquifex aeolicus]
 sp|O66536|YHIT_AQUAE Hypothetical HIT-like protein AQ_141
 pir||E70313 histidine triad-like protein pkcI - Aquifex aeolicus
 gb|AAC06496.1| protein kinase C inhibitor (HIT family) [Aquifex aeolicus VF5]
          Length = 121

 Score =  104 bits (260), Expect = 6e-22,   Method: Compositional matrix adjust.
 Identities = 49/99 (49%), Positives = 72/99 (72%), Gaps = 5/99 (5%)

Query: 2   ETVFSKIIEGSIPSKKVFENERILAFYDIEPAAPVHVLIIPKKFIASMNDVAPEDLPLIG 61
           + +F KI+ G +P+KKV+E++++LAF+DI P APVH+LIIPKK I  +  + PED  L+G
Sbjct: 5   DCIFCKIVRGEVPAKKVYEDDKVLAFHDINPVAPVHILIIPKKHIMGIQTLEPEDECLVG 64

Query: 62  EIHAVAQQIAVELGIA-----DSGYRLINNCGPDSGQAV 95
            +  VA++IA +LGIA     + GYRL+ N G D+GQ+V
Sbjct: 65  HMFYVARKIAEDLGIAPDENLNKGYRLVFNVGKDAGQSV 103


>gb|EAI77744.1| unknown [environmental sequence]
          Length = 114

 Score =  103 bits (256), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 53/94 (56%), Positives = 65/94 (69%)

Query: 2  ETVFSKIIEGSIPSKKVFENERILAFYDIEPAAPVHVLIIPKKFIASMNDVAPEDLPLIG 61
          E +F KII G IP  KVFEN+ ILAF DIEP AP+HVLIIPKK I+++N++  ED  LIG
Sbjct: 3  ECIFCKIINGEIPCNKVFENKHILAFDDIEPQAPIHVLIIPKKHISTINEIQFEDKDLIG 62

Query: 62 EIHAVAQQIAVELGIADSGYRLINNCGPDSGQAV 95
          E+   A++IA   GI D GYR I N    +GQ V
Sbjct: 63 EMFIGAKEIAKIKGIQDQGYRTIFNTNEYAGQTV 96


>gb|EAI94140.1| unknown [environmental sequence]
          Length = 133

 Score =  102 bits (254), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 45/95 (47%), Positives = 70/95 (73%), Gaps = 1/95 (1%)

Query: 1   METVFSKIIEGSIPSKKVFENERILAFYDIEPAAPVHVLIIPKKFIASMNDVAPEDLPLI 60
           ++ +F KII+G IPSKK++E++++ AF+DI P APVH L+IPKK IA++NDV  +D  ++
Sbjct: 22  VDCLFCKIIDGKIPSKKIYEDDQVFAFHDINPQAPVHFLVIPKKHIATLNDVTEDDRAVV 81

Query: 61  GEIHAVAQQIAVELGIADSGYRLINNCGPDSGQAV 95
           G +  +AQ++A ELG  ++G+R + NC    GQ V
Sbjct: 82  GHMVYIAQKLANELG-CETGFRTVFNCNEMGGQTV 115


  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Mar 24, 2004  1:53 AM
  Number of letters in database: 757,237,578
  Number of sequences in database:  2,715,099
  
Lambda     K      H
   0.317    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2715099
Number of Hits to DB: 151,908,431
Number of extensions: 5522812
Number of successful extensions: 12925
Number of sequences better than 1.0e-05: 572
Number of HSP's gapped: 12663
Number of HSP's successfully gapped: 572
Length of query: 119
Length of database: 757,237,578
Length adjustment: 84
Effective length of query: 35
Effective length of database: 529,169,262
Effective search space: 18520924170
Effective search space used: 18520924170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 120 (50.8 bits)