BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0021.p (217 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_981280.1| membrane protein, putative [Bacillus cereus ATC... 125 1e-27 ref|NP_834533.1| hypothetical Membrane Spanning Protein [Bacillu... 110 3e-23 ref|NP_622335.1| conserved hypothetical protein [Thermoanaerobac... 105 9e-22 ref|ZP_00103987.1| COG4194: Predicted membrane protein [Desulfit... 99 6e-20 ref|NP_632407.1| hypothetical protein [Methanosarcina mazei Goe1... 85 1e-15 >ref|NP_981280.1| membrane protein, putative [Bacillus cereus ATCC 10987] gb|AAS43888.1| membrane protein, putative [Bacillus cereus ATCC 10987] Length = 212 Score = 125 bits (313), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 59/157 (37%), Positives = 94/157 (59%), Gaps = 2/157 (1%) Query: 30 KLPDQLPAHFDIRGEADRYWSK-GSVIAFTGFLGLIFPLAMQFIKSVDPKKENYSKFQNA 88 LP+++P+H+++ GE D + SK G +I + I+ L + + +DPK +NY KF Sbjct: 24 NLPEEVPSHWNVSGEVDGHMSKMGGMIFDVAIMVFIYAL-LTVLPKIDPKYKNYDKFSKG 82 Query: 89 YKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIRDNYFTGI 148 Y +I ++ L + ++ + GL +IP + +GLLFIV+GN++PQ + NYF GI Sbjct: 83 YNVINYSVLILLFLVNIIGIGAGLGYDIPMNSTPHILVGLLFIVIGNYLPQCKPNYFVGI 142 Query: 149 RTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLP 185 +T WTL++ EVWRKTHRFSG +++ F P Sbjct: 143 KTPWTLSNEEVWRKTHRFSGKVFVVLGVIMILSIFAP 179 >ref|NP_834533.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] gb|AAP11734.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] Length = 211 Score = 110 bits (274), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 60/174 (34%), Positives = 100/174 (57%), Gaps = 4/174 (2%) Query: 13 IVILGVLSLGYALANYGKLPDQLPAHFDIRGEADRYWSK-GSVIAFTGFLGLIFPLAMQF 71 I+++ + L +A A + LPD + H+ G+ D Y SK +++ G + ++ + + Sbjct: 8 ILLIVITCLAWAFA-WPHLPDTIATHWS-GGKVDGYSSKLYGMLSMVGIM-IVLYIFLNV 64 Query: 72 IKSVDPKKENYSKFQNAYKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFI 131 + +DPKK NY KF A+ M+ I L + ++ GL N+ ++ + +G+LFI Sbjct: 65 LPKIDPKKANYEKFSKAFMMMNNGILLLLFVGNIDIITSGLGYNLFINRVPELLVGILFI 124 Query: 132 VVGNFMPQIRDNYFTGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLP 185 V+GN++PQ + NYF GI+T WTL++ EVWRKTHRFSG +++ F P Sbjct: 125 VIGNYLPQCKPNYFVGIKTPWTLSNEEVWRKTHRFSGKVFVALGIIMILSVFAP 178 >ref|NP_622335.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM23939.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 220 Score = 105 bits (261), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 54/189 (28%), Positives = 88/189 (46%), Gaps = 1/189 (0%) Query: 23 YALANYGKLPDQLPAHFDIRGEADRYWSKGSVIAFTGFLGLIFPLAMQFIKSVDPKKENY 82 + A +G+LP ++P H++I G+ D + K + + +I+ M + +DPK+ NY Sbjct: 27 FTFAIFGRLPAKIPIHWNISGQVDSFGPKHDIFILPSIITIIY-FVMLCVPIIDPKRANY 85 Query: 83 SKFQNAYKMIRLAIAALFDAMLVLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIRD 142 KF AY+MIR + + A+ + L I ++ + I + I G+ M ++R Sbjct: 86 DKFAGAYRMIRAVVVIILSAVYFASAYSVLGYRIDVNRMGNLIIPFMLIGFGSVMGKLRH 145 Query: 143 NYFTGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLPQXXXXXXXXXXXXXXXX 202 NYF GIRT WTLA EVW TH+ + +W+ F Sbjct: 146 NYFVGIRTPWTLADEEVWNNTHQLAEKLWLIAGIIGLFASFFRGTWVTVFMFVLLITAVI 205 Query: 203 XPFAYSWLI 211 P YS++I Sbjct: 206 VPIVYSYII 214 >ref|ZP_00103987.1| COG4194: Predicted membrane protein [Desulfitobacterium hafniense] Length = 221 Score = 99.4 bits (246), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 57/189 (30%), Positives = 92/189 (48%), Gaps = 4/189 (2%) Query: 28 YGKLPDQLPAHFDIRGEADRYWSKGSVIAFTGFLGLIFPLAMQFIKSVDPKKENYSKFQN 87 Y +LP+Q+P+H+++ G+ D Y + + L + I +DP+K NY K Sbjct: 32 YPRLPEQVPSHWNLAGQVDGYSGALTGAFLLPLITLGVYILFWIIPRIDPQKANYLKMGR 91 Query: 88 AYKMIRLAIAALFDAML--VLTVSYGLNQNIPAGKIAMVAIGLLFIVVGNFMPQIRDNYF 145 + ++ + A M + V+ G + +P + IG++FI++GN+ +I+ NY Sbjct: 92 VFWIVSTTLVAFLSLMYWGTIAVALGYLETLP--RWYFSGIGIIFILLGNYFGKIKFNYT 149 Query: 146 TGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLPQXXXXXXXXXXXXXXXXXPF 205 GIRT WTLA+ EVW KTHRF+G +W+ LP P Sbjct: 150 FGIRTPWTLANEEVWAKTHRFAGPIWIVGGILMALAGVLPAAWTEPLFGIVIVLIAVVPM 209 Query: 206 AYSWLISSR 214 AYS+L+ R Sbjct: 210 AYSYLVYRR 218 >ref|NP_632407.1| hypothetical protein [Methanosarcina mazei Goe1] gb|AAM30079.1| hypothetical protein [Methanosarcina mazei Goe1] Length = 213 Score = 85.1 bits (209), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 55/172 (31%), Positives = 83/172 (48%), Gaps = 2/172 (1%) Query: 16 LGVLSLGYALANYGKLPDQLPAHFDIRGEADRYWSKGSVIAFTGFLGLIFPLAMQFIKSV 75 L +LS ++ Y ++PDQ+ H+D +GE + Y SK F L + I + Sbjct: 11 LVLLSFILSIYFYPQVPDQMATHWDSQGEVNGYMSKFWGTFFMPLLITGLVILFLVIPKI 70 Query: 76 DPKKENYSKFQNAYKMIRLAIAALFDAMLVLTVS-YGLNQNIPAGKIAMVAIGLLFIVVG 134 DP+KEN F+ Y+ RL I LF M+ L + + I + + IGLLF G Sbjct: 71 DPRKENIEMFRKHYEGFRL-ILILFLVMVHLHILLWNTGTQISPNAVIPLGIGLLFYYAG 129 Query: 135 NFMPQIRDNYFTGIRTAWTLASPEVWRKTHRFSGVMWMXXXXXXXXXXFLPQ 186 N+F GIRT WTL+S +VW++T+R G ++ F P+ Sbjct: 130 VLTENAEQNWFIGIRTPWTLSSEKVWKRTNRLGGKLFRIAGITAFSGVFFPE 181 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.327 0.140 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 291,104,501 Number of extensions: 10852887 Number of successful extensions: 32253 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 32249 Number of HSP's successfully gapped: 11 Length of query: 217 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 96 Effective length of database: 428,710,599 Effective search space: 41156217504 Effective search space used: 41156217504 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)