BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0100.0003.p (579 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060472.1| COG3858: Predicted glycosyl hydrolase [Clostr... 313 7e-84 ref|ZP_00062427.1| COG3858: Predicted glycosyl hydrolase [Clostr... 147 8e-34 ref|NP_906918.1| conserved hypothetical protein [Wolinella succi... 117 7e-25 ref|NP_623337.1| conserved hypothetical protein [Thermoanaerobac... 114 1e-23 ref|NP_348183.1| Chitinase family protein [Clostridium acetobuty... 105 2e-21 >ref|ZP_00060472.1| COG3858: Predicted glycosyl hydrolase [Clostridium thermocellum ATCC 27405] Length = 583 Score = 313 bits (802), Expect = 7e-84, Method: Compositional matrix adjust. Identities = 174/504 (34%), Positives = 291/504 (57%), Gaps = 32/504 (6%) Query: 87 QKYVDSSIRYEEDTKSVILSTDNELLYMQEDTKTASLNNKPLQLRLAPEEKNGMTYLPAD 146 ++Y D I ++E V ++T + ++ M+ D A +NN+P+ L + +N + Y+P + Sbjct: 75 KEYFDPHIFWDEALGKVTITTKDRVIRMKTDKLDAIVNNQPITLNIPVTVENDVVYIPIE 134 Query: 147 TLENLYGFEVEEDKDTGAVLLMTAGETVPLGKVKGEEGGKTQPLRSEATIHAPIVA--DM 204 L YG EV + + V++ + + E +R +I PI+ D+ Sbjct: 135 FLAEFYGIEVSYVEKSNVVIIDYKNSIKQIAEPIDPEA----VVRKGRSIREPIIRKFDL 190 Query: 205 SPG----TVVKIWNSDNEDWLYVQMDNGFTGYVKAKDIIQDG---EKAVEVKPAAPSRAE 257 S G ++I+ + + W V+ +G GY++ + ++ EK + K P+ Sbjct: 191 SSGDTAENTLRIF-EEYDKWYKVRTWDGAIGYIEKRFVVVKKLMVEKISDDKTPKPA--- 246 Query: 258 RSW---KGKPVNLYWEAVYERKPNPANFGALPGVNVVSPTWFEIVDIKGNVRSKADSAYV 314 W KGK +NL W+ +Y R+ + ++ G + G++V+SPTWF++ + KG + ++A S YV Sbjct: 247 --WTPPKGK-INLAWDMIYTRREDHSSLGEMKGLDVISPTWFQVKNAKGELINRAYSKYV 303 Query: 315 QWAHQQGMEVWGLLTNSF-DADLTTQALSSYEKRMNAIVQMLEYADLYGLDGINIDFENV 373 WAH +G +VW LL+N F D+++T++ L++ + R N I ++L YA LY LDGINIDFEN+ Sbjct: 304 DWAHSRGYQVWALLSNDFTDSEMTSKFLNNTDARDNLIREILAYAALYNLDGINIDFENM 363 Query: 374 YTKDGENVTQFMRELKPMAQAKNLIVSMDVTPKSNSEMWSLFLDRRALGAVTDFLIVMAY 433 Y D + TQF+RE+ P+ + + L+VS+DV D++AL D+++ M+Y Sbjct: 364 YISDRDVFTQFVREIAPLLREQGLVVSVDVNDIQ-------CYDKKALSEAVDYIMYMSY 416 Query: 434 DEHWASSPTAGSVASLPWVEKSVSRILEEDDVPAEKLILGIPLYTRIWSETTKD-GKTKV 492 D+HW++SP AGSVA + W EK V R+LE++ VP EKL+LGIP YTR+W ET + GK K+ Sbjct: 417 DQHWSTSPVAGSVAQVSWQEKIVKRVLEQEGVPREKLLLGIPFYTRLWKETVDESGKKKL 476 Query: 493 SSKAVSMNAVQEILAEKKLKPVLDKDSGQNYVEYTEDGVLRKIWIEDKVSLKARVELAKS 552 +S A++M + ++ E K D++SGQ Y EYT+D ++W+ED S+ R L Sbjct: 477 TSSALTMKQAKNLIIENNAKVEWDEESGQFYAEYTKDNTNYRLWLEDANSINLRTSLVHK 536 Query: 553 FGLGGIGSWNRSFAVPEAWETLQQ 576 + L G +W+ F + W+ L + Sbjct: 537 YRLAGTCAWSIYFVSEDIWDVLNK 560 >ref|ZP_00062427.1| COG3858: Predicted glycosyl hydrolase [Clostridium thermocellum ATCC 27405] Length = 146 Score = 147 bits (370), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 69/152 (45%), Positives = 104/152 (68%), Gaps = 8/152 (5%) Query: 313 YVQWAHQQGMEVWGLLTNSF-DADLTTQALSSYEKRMNAIVQMLEYADLYGLDGINIDFE 371 YV WAH +G +VW LL+N F D+++T++ L++ + R N I ++L YA LY LDGINIDFE Sbjct: 2 YVDWAHSRGYQVWALLSNDFTDSEMTSKFLNNTDARDNLIREILAYAALYNLDGINIDFE 61 Query: 372 NVYTKDGENVTQFMRELKPMAQAKNLIVSMDVTPKSNSEMWSLFLDRRALGAVTDFLIVM 431 N+Y D + TQF+RE+ P+ + + L+VS+DV D++AL D+++ M Sbjct: 62 NMYISDRDVFTQFVREIAPLLREQGLVVSVDVNDIQ-------CYDKKALSEAVDYIMYM 114 Query: 432 AYDEHWASSPTAGSVASLPWVEKSVSRILEED 463 +YD+HW++SP AGSVA + W EK V R+LE++ Sbjct: 115 SYDQHWSTSPVAGSVAQVSWQEKIVKRVLEQE 146 >ref|NP_906918.1| conserved hypothetical protein [Wolinella succinogenes] emb|CAE09818.1| conserved hypothetical protein [Wolinella succinogenes] Length = 341 Score = 117 bits (294), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 87/299 (29%), Positives = 144/299 (48%), Gaps = 16/299 (5%) Query: 288 VNVVSPTWFEIVDIKGNVRSKADSAYVQWAHQQGMEVWGLLTN-SFDADLTTQALSSYEK 346 + VV+P F ++G V + D + A ++G ++ L+ N +F+ + L++ + Sbjct: 47 IEVVAPQVFSFT-LEGTVYGEVDPRIIALAKERGKKLMPLVMNENFEKEKIKAFLANPKA 105 Query: 347 RMNAIVQMLEYADLYGLDGINIDFENVYTKDGENVTQFMRELKPMAQAKNLIVSMDVTPK 406 + I +++ A G G +DFE++ E TQ +R+L + +L +S+ V P Sbjct: 106 QERLIGSLIKIALKEGFGGWQLDFEHLGITSKEAYTQLVRQLYARLKEHHLSLSIAVVPP 165 Query: 407 SNSEM-----------WSLFLDRRALGAVTDFLIVMAYDEHWASSPTAGSVASLPWVEKS 455 E W D +AL +DFL +MAYD+H S T G +AS WVE Sbjct: 166 RGEEPKNAYGAKVFEDWVSPYDLKALAEHSDFLTLMAYDQHTGGS-TPGPIASRAWVEGI 224 Query: 456 VSRILEEDDVPAEKLILGIPLYTRIWSETTKDGKTKVSSKAVSMNAVQEILAEKKLKPVL 515 + L VP EKL LGIPLY+R+W K+ K + +K+++ + L + + + Sbjct: 225 IQEAL--IWVPKEKLSLGIPLYSRLWHAGIKEEKMRGVAKSLTFAGAMDWLERQGAETLW 282 Query: 516 DKDSGQNYVEYTEDGVLRKIWIEDKVSLKARVELAKSFGLGGIGSWNRSFAVPEAWETL 574 + G + GV +++E+ SL +VELAK +GL GI W P W+ L Sbjct: 283 LERDGVKWAMGERGGVKEYLFMEEARSLALKVELAKRYGLRGISLWRLGQEDPRIWDEL 341 >ref|NP_623337.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24941.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 361 Score = 114 bits (284), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 85/302 (28%), Positives = 146/302 (48%), Gaps = 29/302 (9%) Query: 288 VNVVSPTWFEIVDIKGNVRSKADSAYVQWAHQQGMEVWGLLTNSFDADLTTQALSSYEKR 347 ++ +SP W V+ G V+ + + +A +QG++V L+ + D + +K Sbjct: 71 IDTLSPLWLT-VEGDGTVKDSTNPQALNYAKKQGIKVVPLVNVANSKDEVLLDPNIRDKV 129 Query: 348 MNAIVQMLEYADLYGLDGINIDFENV------YTKDGENVTQFMRELKPMAQAKNLIVSM 401 ++ +V +L+ D DG NIDFE + Y KD + +T F+ +LKP+ + + I+ + Sbjct: 130 ISQLVVLLKKHDF---DGYNIDFEFIPHGEKNYVKDKDYLTAFVAKLKPLVKKEGKILDI 186 Query: 402 DVTPKSN--SEMWSLFLDRRALGAVTDFLIVMAYDEHWASSPTAGSVASLPWVEKSVSRI 459 V P E+ ++ D R L + D + +M YD H AS+P G V+ WVE +V Sbjct: 187 SVIPHYQVPKEISGIY-DYRELAHLVDHVTLMTYDRHNASTP-PGPVSPEQWVEYNVKDA 244 Query: 460 LEEDDVPAEKLILGIPLYTRIWSETTKDGKTKVSSKAVSMNAVQEILAEKKLKPV----L 515 L E P E++ LG+ Y W + G ++ +EI+ + KL+ + Sbjct: 245 LNEGFKP-EQICLGVATYGYDWPASKSGGFSR---------PTKEIMTKAKLQGIEIKWS 294 Query: 516 DKDSGQNYVEYTE-DGVLRKIWIEDKVSLKARVELAKSFGLGGIGSWNRSFAVPEAWETL 574 DK Y Y + G R++W E+ +L ++E+AK + L GI W F P W+ + Sbjct: 295 DKYQEPYYTYYDKITGTTREVWFENSYTLAEKIEVAKRYNLHGICVWRIGFETPSFWKVI 354 Query: 575 QQ 576 Q Sbjct: 355 DQ 356 >ref|NP_348183.1| Chitinase family protein [Clostridium acetobutylicum] pir||H97091 chitinase family protein [imported] - Clostridium acetobutylicum gb|AAK79523.1| Chitinase family protein [Clostridium acetobutylicum] Length = 446 Score = 105 bits (263), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 75/282 (26%), Positives = 121/282 (42%), Gaps = 17/282 (6%) Query: 299 VDIKGNVRSKADSAYVQWAHQQGMEVWGLLTNSFDADLTTQALSSYEKRMNAIVQMLEYA 358 D GN+ + V +A+ G++ + N+FD + L + R N I +L Sbjct: 172 TDGVGNISGLVPTNQVTYANNNGIKTLATIENNFDGAVAQTLLENSTNRQNLINNILTSL 231 Query: 359 DLYGLDGINIDFENVYTKDGENVTQFMRELKPMAQAKNLIVSMDV---TPKSNSEMWSLF 415 G G+NID E VY D +T FM EL + V++ V T S + W+ Sbjct: 232 KANGYKGVNIDLEGVYYYDRSYLTTFMNELYSTLNPQGFYVTICVPAKTSDSPTAAWNGA 291 Query: 416 LDRRALGAVTDFLIVMAYDEHW-ASSPTAGSVASLPWVEKSVSRILEEDDVPAEKLILGI 474 D AL D +I+M YDEH+ SP G +AS+ WVE + + +P K++LG Sbjct: 292 FDYAALAKAADQVILMTYDEHYGGGSP--GPIASIGWVENVIKYAITV--IPTTKIMLGA 347 Query: 475 PLYTRIWSETTKDGKTKVSSKAVSMNAVQEILAEKKLKPVLDKDSGQNYVEYTE-DGVLR 533 Y WS +KA ++ + + D S Y Y + G+ Sbjct: 348 AAYGYDWSSN--------PTKAYGISGTYNLASTYNATINWDLTSQSPYFYYVDSSGINH 399 Query: 534 KIWIEDKVSLKARVELAKSFGLGGIGSWNRSFAVPEAWETLQ 575 +W E+ SL +++L S+ L G+ W + W +++ Sbjct: 400 SVWFENGQSLNYKLDLINSYNLSGVAIWRLGLENSDYWTSIR 441 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.131 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 854,946,659 Number of extensions: 36627737 Number of successful extensions: 69688 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 69685 Number of HSP's successfully gapped: 81 Length of query: 579 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 448 Effective length of database: 401,559,609 Effective search space: 179898704832 Effective search space used: 179898704832 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)