BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0099.0009.p (355 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097552.1| COG4632: Exopolysaccharide biosynthesis prote... 203 6e-51 ref|NP_622654.1| conserved hypothetical protein [Thermoanaerobac... 201 2e-50 ref|NP_781452.1| conserved protein [Clostridium tetani E88] >gi|... 197 3e-49 ref|NP_562915.1| conserved hypothetical protein [Clostridium per... 197 4e-49 ref|ZP_00097379.1| COG4632: Exopolysaccharide biosynthesis prote... 188 2e-46 >ref|ZP_00097552.1| COG4632: Exopolysaccharide biosynthesis protein related to N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase [Desulfitobacterium hafniense] Length = 224 Score = 203 bits (516), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 102/224 (45%), Positives = 152/224 (67%), Gaps = 3/224 (1%) Query: 104 VEKVSGSGYTGYVMTVNDPTKVRLGVPNKIG-SGEKVTSMVARTGAIAGVNGGGFADPNW 162 +E + G + G VM ++DP +V+L V +IG +GE+++ MV GAIAG+N GGF DP+ Sbjct: 1 MEDIQGKNFKGKVMLISDPKRVKLAVTQEIGVAGERLSDMVKHAGAIAGINAGGFYDPDG 60 Query: 163 KGNGFKPIGLVISQGKLFYNGLGGKKSTQIVGLDKQGKMIAGNYTLDELSKMGVQEAVTF 222 GNG P G+ + G++ +N G K +G+D +GK+I G ++++ + +QE V+F Sbjct: 61 SGNGAFPDGITVQNGEVVHNN-AGNKVVDFIGIDAEGKLIIGPMDVNQVKEKNIQEGVSF 119 Query: 223 QPRIIVNGKGLIKNAAEGWGIAPRTAMGQRADGALLFVVIDGRQPGYSIGANLYDVQQIM 282 P ++ NGK + GWGIAPRT +GQRADG ++FVVIDGRQP +SIGA L D+ + Sbjct: 120 YPPLVKNGKPAVSGDG-GWGIAPRTGIGQRADGTIIFVVIDGRQPTWSIGATLRDMMNVF 178 Query: 283 LKHGAVIAANLDGGSSTVLVKDNEIVNKPSSQYGERYLPTAFLV 326 L++ AV A NLDGGSS+ +V + +I+NK + +GERY+PT F+V Sbjct: 179 LEYDAVEAVNLDGGSSSEMVYNGKIINKLWNIFGERYIPTGFVV 222 >ref|NP_622654.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM24258.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 314 Score = 201 bits (512), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 113/297 (38%), Positives = 174/297 (58%), Gaps = 10/297 (3%) Query: 32 YFAPSAQNTRYLIADTLITTQHRYLAKYIIGEEELKERVTAYNQRFEHMGDEKDTHVIKE 91 ++ P + +A + T H+YLA + ++ + + ++ + E D ++I Sbjct: 25 FYGPFTRVRDTFVATAMTTFTHQYLATLFLPKKVIDKIMS--ESKIE--SGRTDKNLIHV 80 Query: 92 PAAEEQNKPLVEVEKVSGSGYTGYVMTVNDPTKVRLGVPNKIGSGEKVTSMVART-GAIA 150 +++ +E+ V G + G V+ ++DPT+V +G +K + TS++A+ A+A Sbjct: 81 NGGKDRR---IELYNVYGKHFEGKVILIHDPTRVEVGTSSKFPYEGETTSVIAKHYNALA 137 Query: 151 GVNGGGFADPNWKGNGFKPIGLVISQGKLFYNGLGG-KKSTQIVGLDKQGKMIAGNYTLD 209 +N GGF D + KG G P G VI +GKL Y + + ++G GK++ G Y Sbjct: 138 AINAGGFGDSSMKGIGGAPQGFVIHRGKLLYTEIKNPDEKVDLIGFTNDGKLMVGKYRYK 197 Query: 210 ELSKMGVQEAVTFQPRIIVNGKGLIKNAAEGWGIAPRTAMGQRADGALLFVVIDGRQPGY 269 E+ MG++EAV+F P +++NG+ +IK GWGIAPRTA+GQR DG ++F+VIDGR Sbjct: 198 EVLNMGIKEAVSFGPALVINGQAMIKKGDGGWGIAPRTAIGQRKDGTVIFLVIDGRTLK- 256 Query: 270 SIGANLYDVQQIMLKHGAVIAANLDGGSSTVLVKDNEIVNKPSSQYGERYLPTAFLV 326 SIGA L DVQ IMLK+GA AANLDGGSST + +++N PS GER +PT F V Sbjct: 257 SIGATLKDVQDIMLKYGAYNAANLDGGSSTTMYFKGKVINHPSDALGERAVPTIFFV 313 >ref|NP_781452.1| conserved protein [Clostridium tetani E88] gb|AAO35389.1| conserved protein [Clostridium tetani E88] Length = 335 Score = 197 bits (502), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 108/301 (35%), Positives = 177/301 (58%), Gaps = 12/301 (3%) Query: 32 YFAPSAQNTRYLIADTLITTQHRYLAKYIIGEEELKERVTAYNQRFEHMGDEKDTHVIKE 91 Y+ P + L+ + T H+Y+A + ++++KE + G+ IK+ Sbjct: 39 YYGPFKNLRKNLVGAAMSTLSHQYIATAFLSDKKIKEILQEDVVEVLEQGNS-----IKD 93 Query: 92 PAAEEQNKPLVEVEKVSGSGYTGYVMTVNDPTKVRLGVPNKIGSGEKVTSMVARTG-AIA 150 ++ +E + G + GY++ V+D ++V++G +KI ++TS +A+ AIA Sbjct: 94 VQFNNKHDKNIERYDIDGGKFKGYMLVVHDASRVKVGHSSKIPKSGELTSKIAKDNKAIA 153 Query: 151 GVNGGGFADPN----WKGNGFKPIGLVISQGKLFYNGLGG-KKSTQIVGLDKQGKMIAGN 205 +NGGGF D N W G G +P+G+++S G+L +N + K ++ + +G ++ G Sbjct: 154 AINGGGFTDKNDDGNWTGTGGQPVGILMSNGELVHNDIKDLNKKEDVMAITSKGVLLVGK 213 Query: 206 YTLDELSKMGVQEAVTFQPRIIVNGKGLIKNAAEGWGIAPRTAMGQRADGALLFVVIDGR 265 Y+L+E+ + V+EA+TF P ++VNG+ IK+ G G APRTA+GQR DG++L +V+DGR Sbjct: 214 YSLNEMEDLDVKEAITFGPALVVNGQPTIKSGNGGQGPAPRTAIGQRKDGSILLLVVDGR 273 Query: 266 QPGYSIGANLYDVQQIMLKHGAVIAANLDGGSSTVLVKDNEIVNKPSSQYGERYLPTAFL 325 Q SIGA L D+Q IML++GA A NLDGGSS + D E++N P GER +P+ Sbjct: 274 QF-RSIGATLKDIQDIMLEYGAYNATNLDGGSSATMYYDGEVINNPCDPLGERSIPSVVY 332 Query: 326 V 326 V Sbjct: 333 V 333 >ref|NP_562915.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB81705.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 336 Score = 197 bits (500), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 109/301 (36%), Positives = 176/301 (58%), Gaps = 11/301 (3%) Query: 33 FAPSAQNTRYLIADTLITTQHRYLAKYIIGEEELKERVTAYNQRFEHMG-DEKDTHVIKE 91 + P R + + + H+++A + +E++ E + N E + K+T++ Sbjct: 39 YGPFENAKRTYVGAAMTSFSHQWMATTFLSDEKINEIL---NSNIEDTNTNHKNTNIKTN 95 Query: 92 PAAEEQNKPLVEVEKVSGSGYTGYVMTVNDPTKVRLGVPNKIGSGEKVTSMVART-GAIA 150 ++ +E+ Y+GY + V DPT+V++GV +G + TS +AR A+A Sbjct: 96 VNLPTKHDNSIELYSFENFKYSGYYIVVKDPTRVKIGVSKYLGEEGQTTSEIAREYNAVA 155 Query: 151 GVNGGGFADPN----WKGNGFKPIGLVISQGKLFYNGLGGKKSTQIVGLDKQGKMIAGNY 206 VNGG F D + W GNG P G+VIS+GKL Y + + ++VG+ K+GKMIAG Y Sbjct: 156 AVNGGAFTDKSSTAQWTGNGGTPAGIVISEGKLVYKDVPDDEKIELVGITKEGKMIAGMY 215 Query: 207 TLDELSKMGVQEAVTFQPRIIVNGKGLIKNAAEGWGIAPRTAMGQRADGALLFVVIDGRQ 266 + + L ++ V+EAV+F P ++ G+ GWG+APRTAMGQRADG+++ +VIDGR Sbjct: 216 SFNNLKELNVKEAVSFGPVLVKEGEPTPMKGDGGWGVAPRTAMGQRADGSIVMLVIDGRS 275 Query: 267 PGYSIGANLYDVQQIMLKHGAVIAA-NLDGGSSTVLVKDNEIVNKPSSQYGERYLPTAFL 325 S GA L ++Q+++L V+ A NLDGG ST + + +++N P+S GER +P+A + Sbjct: 276 L-TSGGATLKELQEVLLNTCNVVTAINLDGGKSTTMYLNGKVINNPASNVGERSIPSAII 334 Query: 326 V 326 V Sbjct: 335 V 335 >ref|ZP_00097379.1| COG4632: Exopolysaccharide biosynthesis protein related to N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase [Desulfitobacterium hafniense] Length = 747 Score = 188 bits (477), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 99/233 (42%), Positives = 146/233 (62%), Gaps = 8/233 (3%) Query: 102 VEVEKVSGSGYTGYVMTVNDPTKVRLGVPNKIGS-GEKVTSMVARTGAIAGVNGGGFADP 160 +E + + G G VM ++DP +V L V +IG+ EK+T MV+R+GAIAG+N GG Sbjct: 515 IESQNLHGFNIKGKVMLISDPKRVTLAVTEEIGTVEEKLTDMVSRSGAIAGINAGGIYLS 574 Query: 161 NWKGNGFKPIGLVISQGKLFYNGLGGKKSTQIVGLDKQGKMIAGNYTLDELSKMGVQEAV 220 +GN P G+ + G++ YN G ++ + +GLD +GK+I G + E+ + +QE V Sbjct: 575 LEEGNEVFPDGITVQNGEVVYNN-AGDQAVEFIGLDAEGKLITGPMNVQEIKEKNIQEGV 633 Query: 221 TFQPRIIVNGKGLIKNAAE------GWGIAPRTAMGQRADGALLFVVIDGRQPGYSIGAN 274 F P + NG L++ GWGIAPR +GQRADG L+F+VIDGR P +SIGA Sbjct: 634 GFSPPLADNGTTLVREGKPAVPGDGGWGIAPRAGIGQRADGTLIFMVIDGRDPDWSIGAT 693 Query: 275 LYDVQQIMLKHGAVIAANLDGGSSTVLVKDNEIVNKPSSQYGERYLPTAFLVF 327 L D++ + L++GAV A NL GGS +V D +++NK S+ +GER +PT F+V Sbjct: 694 LKDMENLFLEYGAVEAVNLSGGSMVEMVYDGKVLNKVSNIFGERPIPTGFVVM 746 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 522,254,540 Number of extensions: 22682056 Number of successful extensions: 57274 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 57251 Number of HSP's successfully gapped: 22 Length of query: 355 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 228 Effective length of database: 412,420,005 Effective search space: 94031761140 Effective search space used: 94031761140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)