BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0088.0001.p (399 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00180480.1| hypothetical protein Mther157301 [Moorella th... 339 7e-92 ref|NP_970124.1| branched-chain amino acid ABC transporter, amin... 321 2e-86 ref|ZP_00013198.1| COG0683: ABC-type branched-chain amino acid t... 260 4e-68 ref|NP_786312.1| branched-chain amino acid ABC transporter, subs... 257 3e-67 ref|NP_345248.1| branched-chain amino acid ABC transporter, amin... 240 4e-62 >ref|ZP_00180480.1| hypothetical protein Mther157301 [Moorella thermoacetica ATCC 39073] Length = 399 Score = 339 bits (870), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 174/357 (48%), Positives = 236/357 (66%), Gaps = 3/357 (0%) Query: 43 IKIGADLELTGGQASFGDSASKGAKLAVQQINDAGGILG-KKLELVVADNASKSEEATQV 101 I IG + EL+G A++G ++ LA ++IN GG+LG KK+ +V DN K EEA Sbjct: 42 INIGVNYELSGDVATYGTNSKDAILLAFEEINQKGGVLGGKKINAIVLDNGGKKEEAMSA 101 Query: 102 AQKLITTDKVVTIIGASTSTNTLGIVPVATEKEIPLVSVGATNPKVTVDERTSKVNDWVF 161 A KLIT +KVV ++G +TS T+ +AT+ ++P++S AT+P VTVD ++ KV D+VF Sbjct: 102 AAKLITENKVVALLGPATSGATMAASTMATKYKVPIISPSATSPDVTVDPQSKKVRDYVF 161 Query: 162 RAAFIDPFQGQVMANFAVDSLKAKTAVIYTDTSSDYSKGLQKFFEETFKSKGGEVLSQES 221 R FIDP Q V A FA L AK A I S +YSKGL K F+E F GG+V+++ES Sbjct: 162 RVCFIDPTQAIVGAEFATKDLGAKKAAIIYSNSDEYSKGLYKVFKEEFAKDGGQVVAEES 221 Query: 222 YQQKDSDFKAVLTRIKAANPDVIYLPGYYEEVGKILKQAREMGITAPFLGGDGWDSPQLA 281 + D DF+ LTR+K A D+IY+P YY +VGKI+ QARE+GI P LG DGWDSP+LA Sbjct: 222 FNSGDQDFRPALTRVKDAGADLIYVPAYYGDVGKIVNQARELGIKVPMLGADGWDSPKLA 281 Query: 282 EIAGADA-LNDTYMSNHYSPEDTATEVTSFVDAYKAANGGAVPDGMAALGYDALKLVADA 340 E AG A LN+TY +NHY+ D + +F+ A+KA PD AAL YD L+ADA Sbjct: 282 EYAGGAANLNNTYFTNHYAASDPNPRLQAFIKAFKAKYNKE-PDAFAALAYDTAYLLADA 340 Query: 341 ITRAGAADPAKIKDALAATKDLQLATGKITLNETHDPVKAAVVLKFVDGKQTFETKV 397 I RAG+ADP I++ALA+TKD + T KIT+++ H+PVK ++ VDGKQT + K+ Sbjct: 341 INRAGSADPEAIRNALASTKDFEGITSKITIDQNHNPVKDVAIIAMVDGKQTLKKKI 397 >ref|NP_970124.1| branched-chain amino acid ABC transporter, amino acid-binding protein [Bdellovibrio bacteriovorus HD100] emb|CAE78183.1| branched-chain amino acid ABC transporter, amino acid-binding protein [Bdellovibrio bacteriovorus] Length = 373 Score = 321 bits (823), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 172/357 (48%), Positives = 223/357 (62%), Gaps = 8/357 (2%) Query: 43 IKIGADLELTGGQASFGDSASKGAKLAVQQINDAGGILGKKLELVVADNASKSEEATQVA 102 I IG LTG A+FG S++KG +LA+ +IN AGG+ GKK+ LV D+ K+EEA Sbjct: 25 IVIGEYDSLTGSDATFGLSSNKGVRLALDEINAAGGVKGKKISLVTLDDQGKNEEAASAV 84 Query: 103 QKLITTDKVVTIIGASTSTNTLGIVPVATEKEIPLVSVGATNPKVTVDERTSKVNDWVFR 162 +LIT + VV IIG S + P+A ++P VS +TNP VT K D+VFR Sbjct: 85 TRLITQNNVVAIIGGVASGRSKAAAPIAQSNKVPFVSPASTNPDVT------KAGDYVFR 138 Query: 163 AAFIDPFQGQVMANFAVDSLKAKTAVIYTDTSSDYSKGLQKFFEETFKSKGGEVLSQESY 222 FIDPFQG VMA FA ++LK K A I D +DYS GL F FK GGE+++ SY Sbjct: 139 VCFIDPFQGMVMAKFAKENLKIKKAAILRDVKNDYSVGLADVFAAEFKKGGGEIVADLSY 198 Query: 223 QQKDSDFKAVLTRIKAANPDVIYLPGYYEEVGKILKQAREMGITAPFLGGDGWDSPQLAE 282 Q D DFKA LT+I++ NP+ IY+PGYY EVG I +QAR++G+ P +GGDGWDS +L E Sbjct: 199 QAGDIDFKAQLTQIRSKNPEAIYVPGYYTEVGLIAQQARQLGVKVPLMGGDGWDSDKLYE 258 Query: 283 IAGADALNDTYMSNHYSPEDTATEVTSFVDAYKAANGGAVPDGMAALGYDALKLVADAIT 342 I G +A+N Y SNHY+ E T VT F+ +KA PD +AALGYDA K++ AI Sbjct: 259 I-GKEAINGNYYSNHYTTESTDPAVTEFIKKFKAKY-NETPDALAALGYDAAKILVAAIE 316 Query: 343 RAGAADPAKIKDALAATKDLQLATGKITLNETHDPVKAAVVLKFVDGKQTFETKVNP 399 RA I+D L+ TKD TGKI+LNE D VK+AVV++ + F T +NP Sbjct: 317 RAPDLSGKAIRDELSKTKDFPGVTGKISLNENRDAVKSAVVIQVDGNNRKFVTTINP 373 >ref|ZP_00013198.1| COG0683: ABC-type branched-chain amino acid transport systems, periplasmic component [Rhodospirillum rubrum] Length = 392 Score = 260 bits (664), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 148/350 (42%), Positives = 211/350 (60%), Gaps = 10/350 (2%) Query: 43 IKIGADLELTGGQASFGDSASKGAKLAVQQINDAGGILGKKLELVVADNASKSEEATQVA 102 IKIG LTGGQAS A GA+LA+ +N AGG+ G LELVV D + + Sbjct: 32 IKIGGAFNLTGGQASLDGPAKNGAQLAIDTLNAAGGVNGTPLELVVYDGKTDPAVVASLG 91 Query: 103 QKLITTDKVVTIIGASTSTNTLGIVPVATEKEIPLVSVGATNPKVTVDERTSKVNDWVFR 162 +LI +DKV IIG S S L + P A + +P ++VGAT+PK+ ++ D +F Sbjct: 92 SQLINSDKVSAIIGFSDSDPVLALGPNAQKAGVPFIAVGATSPKLP-----DQIGDTMFL 146 Query: 163 AAFIDPFQGQVMANFAVDSLKAKTAVIYTDTSSDYSKGLQKFFEETFKSKGGEVLSQESY 222 A F D Q V A+FA+D LKAK+ + DT+++Y+ L K+F ETF+ GG+V+ +++Y Sbjct: 147 ACFGDNTQAAVGASFAIDDLKAKSVYVLEDTANEYATLLSKYFRETFEHLGGKVIGRDTY 206 Query: 223 QQKDSDFKAVLTRIKAA--NPDVIYLPGYYEEVGKILKQAREMGITAPFLGGDGWDSPQL 280 + D F A +T+IKAA PD++Y+ + +G +++Q R+ G+ P +GGDG+D+P L Sbjct: 207 RTGDKSFTAQITKIKAAAEKPDILYIAATPDSIGLVVRQVRQAGLKLPIVGGDGYDTPLL 266 Query: 281 AEIAGADALNDTYMSNHYSPEDTAT-EVTSFVDAYKAANGGAVPDGMAALGYDALKLVAD 339 E+ GA A N+ Y + H D+A + +F DAY A G A + AALGYD + LVAD Sbjct: 267 LEVGGASA-NNVYFTTHSFVSDSAPGPMKTFFDAYGKAYGNAPENAFAALGYDTVMLVAD 325 Query: 340 AITRAGAADPAKIKDALAATKDLQLATGKITLNE-THDPVKAAVVLKFVD 388 AI RAG+ DPA I+ AL TKDL TG +T E + PVK+ V+ D Sbjct: 326 AIKRAGSGDPAAIRKALTETKDLAGVTGAVTFAEGSRVPVKSVTVIGVKD 375 >ref|NP_786312.1| branched-chain amino acid ABC transporter, substrate binding protein [Lactobacillus plantarum WCFS1] emb|CAD65170.1| branched-chain amino acid ABC transporter, substrate binding protein [Lactobacillus plantarum WCFS1] Length = 397 Score = 257 bits (657), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 137/353 (38%), Positives = 214/353 (60%), Gaps = 11/353 (3%) Query: 43 IKIGADLELTGGQASFGDSASKGAKLAVQQINDAGGI----LGKKLELVVADNASKSEEA 98 IKIG ++EL+G A +G+ +G +LAV++IN +GGI KK++LV+ DN + + A Sbjct: 41 IKIGVNMELSGSAAGYGEQQKQGIQLAVKKINKSGGIKVGGTKKKIQLVIRDNKTSNTTA 100 Query: 99 TQVAQKLITTDKVVTIIGASTSTNTLGIVPVATEKEIPLVSVGATNPKVTVDERTSKVND 158 VA +L+ DKVV ++G +T++ +P T+ +P VS AT+P T+ +++ KV Sbjct: 101 ASVAAQLVNNDKVVAVVGPATTSAGTAAIPNMTKAAVPSVSPSATDPDYTL-QKSGKVQK 159 Query: 159 WVFRAAFIDPFQGQVMANFAVDSLKAKTAVIYTDTSSDYSKGLQKFFEETFKSKGGEVLS 218 ++FRA + + FQG A F +LKAK I TD SSDY GL K F++ ++ G V+S Sbjct: 160 YIFRACYQNNFQGATAATFVTKNLKAKRVAILTDNSSDYGTGLTKAFKKQYR---GTVVS 216 Query: 219 QESYQQKDSDFKAVLTRIKAANPDVIYLPGYYEEVGKILKQAREMGITAPFLGGDGWDSP 278 +++Q+ D DF A+LT +K D IY PGYY E+G I+KQAR+ GI AP +G DG Sbjct: 217 SQTFQEGDKDFNAILTALKHKKIDAIYAPGYYSEIGLIVKQARQAGIKAPIVGSDGMADA 276 Query: 279 QLAEIAGADALNDTYMSNHYSPEDTATE--VTSFVDAYKAANGGAVPDGMAALGYDALKL 336 +LA+IAG + Y + +S + +AT + F+ AYKA G P +AL YD++ + Sbjct: 277 KLAQIAGKSNATNVYYTTPFSTKASATNKVASKFMSAYKAKYGSTAPT-FSALAYDSVYM 335 Query: 337 VADAITRAGAADPAKIKDALAATKDLQLATGKITLNETHDPVKAAVVLKFVDG 389 + AI + G+A+ KI D LA K+ + TG +T+++ H+P K + + +G Sbjct: 336 IKAAIEKEGSANSVKIADGLAKLKNFKGVTGTMTIDKQHNPEKTISIEQLTNG 388 >ref|NP_345248.1| branched-chain amino acid ABC transporter, amino acid-binding protein [Streptococcus pneumoniae TIGR4] ref|NP_358253.1| ABC transporter substrate-binding protein - branched chain amino acid transport [Streptococcus pneumoniae R6] pir||G95086 hypothetical protein SP0749 [imported] - Streptococcus pneumoniae (strain TIGR4) pir||C97954 hypothetical protein livJ [imported] - Streptococcus pneumoniae (strain R6) gb|AAK74888.1| branched-chain amino acid ABC transporter, amino acid-binding protein [Streptococcus pneumoniae TIGR4] gb|AAK99463.1| ABC transporter substrate-binding protein - branched chain amino acid transport [Streptococcus pneumoniae R6] Length = 386 Score = 240 bits (613), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 139/349 (39%), Positives = 215/349 (61%), Gaps = 13/349 (3%) Query: 43 IKIGADLELTGGQASFGDSASKGAKLAVQQINDAGGILGKKLELVVADNASKSEEATQVA 102 IKIG + E +G A++G + KGA+LAV +IN AGGI GK++E+V DN S++ EA V Sbjct: 41 IKIGFNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVT 100 Query: 103 QKLITTDKVVTIIGASTSTNTLGIVPVATEKEIPLVSVGATNPKVTVDERTSKVNDWVFR 162 L+T KV ++G +TS T V AT+ +PL+S AT +T K D++F Sbjct: 101 TNLVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLT------KGQDYLFI 154 Query: 163 AAFIDPFQGQVMANFAVDSLKAKTAVIYTDTSSDYSKGLQKFFEETFKSKGGEVLSQESY 222 F D FQG++++N+ + L AK V+YTD +SDY+KG+ K F E++K GE+++ E++ Sbjct: 155 GTFQDSFQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYK---GEIVADETF 211 Query: 223 QQKDSDFKAVLTRIKAANPDVIYLPGYYEEVGKILKQAREMGITAPFLGGDGWDSPQLAE 282 D+DF+A LT++K + D I +PGYY E GKI+ QAR MGI P +GGDG++ + + Sbjct: 212 VAGDTDFQAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQ 271 Query: 283 IAGADALNDTYMSNHYSPE-DTATEVTSFVDAYKAANGGAVPDGMAALGYDALKLVADAI 341 A A+ ++ Y + +S + + + +F+DAY+A P AAL YD++ LVA+A Sbjct: 272 QATAEKASNIYFISGFSTTVEVSAKAKAFLDAYRAKY-NEEPSTFAALAYDSVHLVANAA 330 Query: 342 TRAGAADPAKIKDALAATKDLQLATGKITLNETHDPVKAAVVLKFVDGK 390 GA + +IK+ LA TKD + TG+ + + H+ VK A ++ +GK Sbjct: 331 K--GAKNSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGK 377 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.129 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 504,816,543 Number of extensions: 19667695 Number of successful extensions: 54188 Number of sequences better than 1.0e-05: 942 Number of HSP's gapped: 52850 Number of HSP's successfully gapped: 944 Length of query: 399 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 271 Effective length of database: 409,704,906 Effective search space: 111030029526 Effective search space used: 111030029526 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 127 (53.5 bits)