BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0016.p (137 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_782675.1| hypothetical protein CTC02114 [Clostridium teta... 144 9e-34 >ref|NP_782675.1| hypothetical protein CTC02114 [Clostridium tetani E88] gb|AAO36612.1| hypothetical protein [Clostridium tetani E88] Length = 416 Score = 144 bits (362), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 67/128 (52%), Positives = 95/128 (74%), Gaps = 1/128 (0%) Query: 7 IDEFTDKLNKKANGGSYVVEEKLFLTDGVYSGLLAHDNINNQSIAVYTGSRYSGVELRNF 66 ID+FT+KLNK +G +YV+EE++ L DGVY G L HDNI+N S+ VYTG + +G ++ NF Sbjct: 4 IDKFTEKLNK-IDGNTYVIEEEVELKDGVYEGELQHDNISNTSVRVYTGPKLTGNKIENF 62 Query: 67 SVSFPDETPWRRMIKIFAEVPEVYVTYETPGDTVEADDINVLQWGLTAAQRELERYKATG 126 +S P TPW++ IKIFA+V + Y+TYETPGDTVEADDIN +Q + Q E+E+YKA+ Sbjct: 63 ILSTPSLTPWKKEIKIFAKVDKCYITYETPGDTVEADDINKVQESIVNTQIEVEKYKASN 122 Query: 127 RIDGGSFR 134 ++ + + Sbjct: 123 NLEICNLK 130 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 220,432,267 Number of extensions: 8962717 Number of successful extensions: 18480 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 18479 Number of HSP's successfully gapped: 1 Length of query: 137 Length of database: 757,237,578 Length adjustment: 99 Effective length of query: 38 Effective length of database: 488,442,777 Effective search space: 18560825526 Effective search space used: 18560825526 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 120 (50.8 bits)