BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0081.0012.p (320 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099269.1| hypothetical protein [Desulfitobacterium hafn... 301 1e-80 ref|ZP_00061152.1| hypothetical protein [Clostridium thermocellu... 206 4e-52 ref|NP_945297.1| hypothetical protein EJ-1p58 [Bacteriophage EJ-... 168 2e-40 ref|NP_390478.1| similar to phage-related protein [Bacillus subt... 114 3e-24 ref|NP_389152.1| PBSX prophage [Bacillus subtilis] >gi|1722874|s... 111 3e-23 >ref|ZP_00099269.1| hypothetical protein [Desulfitobacterium hafniense] Length = 323 Score = 301 bits (771), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 142/322 (44%), Positives = 220/322 (68%), Gaps = 2/322 (0%) Query: 1 MELLLKNKEGLLWDIAGIVSDLSWKTSRAGKPATLDMTIIDSGIYQHPKFKISNGDIVQF 60 +++++ NK+G +WDI+ IV+D++WKTSR GK +LD T+I + Q FK +NG+IV Sbjct: 2 LKIVIDNKDGNVWDISQIVADVNWKTSRIGKAGSLDFTLIKNAPAQELAFKYANGNIVHV 61 Query: 61 R-KDGINVFYGFVFSIDTGSDQQIKLTAYDQIRYLLGNGSYVLQDVTASELISKIAKDYG 119 + +DG NVFYG++FSID G D+ +K+T YDQ+RYL+ + + + ++T SEL+ +IA + Sbjct: 62 QMEDGTNVFYGYIFSIDGGKDEAVKITCYDQLRYLMASDTSIYTNITVSELVREIAARFQ 121 Query: 120 LKTGVLEETTYRMPSLIEDDKKLLDIIMRAIGGELEKKGRLMAFYDDFGQLTLRGMDSMK 179 L+TG +++T YR+P+ ED++K++D+I +A+ L G+ F+DDFG LT+R ++ Sbjct: 122 LETGRIDDTKYRIPTFSEDNQKMMDMICKALDLTLINTGKNYVFFDDFGALTIRNVEDFL 181 Query: 180 LDLVLGAGHYLYDYSLKKSIDDDTYNTIYLYKDNKESGKREFYPVSDPENVKRWGILHLY 239 LD ++G + D+S K SID DTYN I LYKDNKE+GKRE Y N+ +WG+L L Sbjct: 182 LDFIIGDHSLMTDFSHKVSIDSDTYNVINLYKDNKETGKREPYEKPCSANIAKWGVLQLC 241 Query: 240 KKADDKANAAQIQENADNLLKLHNREKTSLSVQAIGDLRVRAGNLIYVLLDALD-TRLFL 298 + D++ N AQI E NLL L NRE SL ++A+GD+RVR G + + + + FL Sbjct: 242 QSIDEEMNEAQISEMLKNLLTLKNRETKSLKIEALGDIRVRGGCYVRIQIAEYGINQPFL 301 Query: 299 VEQCSHKISGGEHTMSLDIKVV 320 V++C+H+ G HTMSL+++V+ Sbjct: 302 VDECTHRFDGVNHTMSLELRVI 323 >ref|ZP_00061152.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 314 Score = 206 bits (525), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 113/321 (35%), Positives = 189/321 (58%), Gaps = 11/321 (3%) Query: 1 MELLLKNKEGLLWDIAGIVSDLSWKTSRAGKPATLDMTIIDSGIYQHPKFKISNGDIVQF 60 ME ++ +G +++I+ +V+ +S+ + L+ + ID + KI NG +V+F Sbjct: 3 MEFAVET-DGKIYEISELVTSVSYTDKLNDGCSKLEFSYIDDDL------KIHNGSVVRF 55 Query: 61 RKDGINVFYGFVFSIDTGSDQQIKLTAYDQIRYLLGNGSYVLQDVTASELISKIAKDYGL 120 R D NVFYG+VF ++I +TAYDQ+RY + V+++ T L+ K+ + L Sbjct: 56 RYDNENVFYGYVFKHGQNKAKEISVTAYDQLRYCKAKDTIVVKNDTVDSLVKKMCNYFNL 115 Query: 121 KTGVLEETTYRMPSLIEDDKKLLDIIMRAIGGELEKKGRLMAFYDDFGQLTLRGMDSMKL 180 K G+L T Y++P ++DDK LDII AI L GR D+FG + +R + ++L Sbjct: 116 KAGILTNTGYKLPVSVQDDKTWLDIIYTAISDTLMNTGRWYCLRDEFGNIAVRDLQELQL 175 Query: 181 DLVLGAGHYLYDYSLKKSIDDDTYNTIYLYKDNKESGKREFYPVSDPENVKRWGILHLYK 240 DL+LG YDY +KSIDD+ YN I + DN+++GKR+ Y D +++++G+L ++ Sbjct: 176 DLILGDESLAYDYEYEKSIDDEFYNQIKIVSDNEKTGKRDVYITKDSSSIQKYGLLQYFE 235 Query: 241 KADDKANAAQIQENADNLLKLHNREKTSLSVQAIGDLRVRAGNLIYVLLD--ALDTRLFL 298 D N +Q + AD LL+L+NRE +L ++ +GD+RVRAG+ Y ++ L+ RL + Sbjct: 236 VLDKNYNPSQAKAKADALLRLYNREVETLELECLGDVRVRAGSSFYGRIEDIQLNKRL-I 294 Query: 299 VEQCSHKISGGEHTMSLDIKV 319 V+ +H+ HTM L++ + Sbjct: 295 VKSVAHEFIPV-HTMKLEVMI 314 >ref|NP_945297.1| hypothetical protein EJ-1p58 [Bacteriophage EJ-1] emb|CAE82140.1| hypothetical protein [Bacteriophage EJ-1] Length = 322 Score = 168 bits (425), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 113/330 (34%), Positives = 177/330 (53%), Gaps = 20/330 (6%) Query: 1 MELLLKN-KEGLLWDIAGIVSDLSWKTSRAGKPATLDM-TIIDSGIYQHPKFKISNGDIV 58 ME L+++ +G ++DI +V + W+TS +P L+ IIDS + + GD++ Sbjct: 2 MEFLIQDVNDGKVFDITELVGGVKWETSIDFQPGKLEFDMIIDSQV------ACNFGDVI 55 Query: 59 QFRKDGINVFYGFVFSIDTGSDQQIKLTAYDQIRYLLGNGSYVLQDVTASELISKIAKDY 118 +F+ D +FYG VF S ++ +TAYD++RYL + V + + E+ SKI + Sbjct: 56 RFKVDDKGIFYGKVFKKKRKSAKKWSVTAYDRMRYLKNTDTIVFEASKSHEIFSKICEIS 115 Query: 119 GLKTGVLEETTYRMPSLIEDDKKLLDIIMRAIGGELEKKGRLMAFYDDFGQLTLRGMDSM 178 L+ V++E + P IED K +I A+ L G D+FG + ++S+ Sbjct: 116 ELEYKVVDEGNWTCPEKIEDKKTYFAMIQNALDLTLIHGGMWYIIRDNFGTVEHIALNSL 175 Query: 179 KLDLVLGAGHYLYDYSLKKSIDDDTYNTIYLYKDNKESGKREFYPVSDPENVKRWGILHL 238 DLV+G D+ + SIDD ++N + L KDNKES KRE Y V D +NV WG L Sbjct: 176 ITDLVIGDDSVATDFDYEGSIDD-SFNYVKLTKDNKESKKREVYVVKDSKNVALWGKLQY 234 Query: 239 YKKADDKANAAQIQENADNLLKLHNREKTSLSVQAIGDLRVRAGNLIYVLLDALD----- 293 ++K D+K N +QIQ+ A+ LLK N K + V +G L + AGN V+LD D Sbjct: 235 HEKVDEKMNESQIQQKAELLLKAKNHPKKTFKVPCLGHLGISAGN--SVVLDFADLESEG 292 Query: 294 ---TRLFLVEQCSHKISGGEHTMSLDIKVV 320 L ++ +C+HK HTM L+++ + Sbjct: 293 IKKNSLGIISKCTHKWDRV-HTMDLELRTL 321 >ref|NP_390478.1| similar to phage-related protein [Bacillus subtilis] sp|P45950|YQBQ_BACSU Hypothetical protein yqbQ pir||D69948 phage-related protein homolog yqbQ - Bacillus subtilis dbj|BAA06949.1| ORF86 [Bacillus subtilis] dbj|BAA12413.1| YqbQ [Bacillus subtilis] emb|CAB14542.1| yqbQ [Bacillus subtilis subsp. subtilis str. 168] Length = 326 Score = 114 bits (285), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 83/280 (29%), Positives = 138/280 (49%), Gaps = 18/280 (6%) Query: 47 HPKFKISNGDIVQFRKDGINVFYGFVFSIDTGSDQQIKLTAYDQIRYLLGNGS-YVLQDV 105 H + +S GD V F+ G +F G VFS + + + TAYD ++YL+ N YV + Sbjct: 44 HSYYSVSEGDTVLFKWKGKELFRGIVFSRNP-EEHWMTFTAYDMLQYLVKNKDVYVFSNQ 102 Query: 106 TASELISKIAKDYGLKTGVLEETTYRMPSLI-EDDKKLLDIIMRAIGGELEKKGRLMAFY 164 A E+I ++A+D+ + T + T Y + SL+ +DD L D+I++A+ + GR Y Sbjct: 103 RADEIIKRLARDFQIPTTSIANTGYTIKSLVFKDDTSLYDMILKALKQTKSQTGRNYQLY 162 Query: 165 DDFGQLTLRGMDSMKLDLVLGAGHYLYDYSLKKSIDDD-TYNTIYLYKDNKESGKREFYP 223 G+L LR + VL G + Y SI+D T + KDNK Sbjct: 163 SAKGKLGLRAWPDLSEVWVLETGVNITGYQYSTSINDTATKVKLRRQKDNKTYTA----T 218 Query: 224 VSDPENVKRWGILHLYKKADDKANAAQIQENADNLLKLHNREKTSL-----SVQAIGDLR 278 SD + ++G+L + D N AQ+QE A K+ +K + S+QAIG Sbjct: 219 ASDSSGISKYGVLQYVETVSDNINQAQLQERA----KVKQAQKKGVKKELKSIQAIGIPD 274 Query: 279 VRAGNLIYVLLDALDT-RLFLVEQCSHKISGGEHTMSLDI 317 +++G IY+ + + + + ++ H+ G HTM++D+ Sbjct: 275 LQSGLPIYISIPEVGVKKTYWIDTDKHEFKGSTHTMTIDV 314 >ref|NP_389152.1| PBSX prophage [Bacillus subtilis] sp|P54336|XKDQ_BACSU Phage-like element PBSX protein xkdQ pir||H69732 PBSX prophage ORF xkdQ - Bacillus subtilis emb|CAA94050.1| xkdQ [Bacillus subtilis] emb|CAB13127.1| xkdQ [Bacillus subtilis subsp. subtilis str. 168] Length = 325 Score = 111 bits (277), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 89/307 (28%), Positives = 151/307 (49%), Gaps = 15/307 (4%) Query: 18 IVSDLSWKTSRAGKPATLDMTIIDSGIYQHPKFKISNGDIVQFRKDGINVFYGFVFSIDT 77 + +S + R P +++ TI+ Q + +S GD V F+ G +F G VF+ T Sbjct: 16 VAESVSLEGHRYQAPRSIEATIVTKQGDQ-TYYSVSEGDTVLFKWKGKELFRGIVFA-RT 73 Query: 78 GSDQQIKLTAYDQIRYLLGNGS-YVLQDVTASELISKIAKDYGLKTGVLEETTYRMPSL- 135 + + +AYD ++YL+ N YV + A ++I +IA D+ + T + T + + SL Sbjct: 74 PDEHTLAFSAYDMLQYLVKNQDMYVFSNQRADQIIRRIASDFQIPTTSIANTGHTIKSLV 133 Query: 136 IEDDKKLLDIIMRAIGGELEKKGRLMAFYDDFGQLTLRGMDSMKLDLVLGAGHYLYDYSL 195 I++D L DII++A+ + GR Y + G+L LR VL G + Y Sbjct: 134 IKNDTTLYDIILKALKQTKSQTGRHYQLYSEKGKLGLRAWPDPSEVWVLETGVNITGYQY 193 Query: 196 KKSIDDD-TYNTIYLYKDNKESGKREFYPVS--DPENVKRWGILHLYKKADDKANAAQIQ 252 SI+D T + KDNK Y S D + ++G+L + D N AQ+Q Sbjct: 194 STSINDTATRVVLRRQKDNKT------YKASAKDSSGLNKYGVLQYTETVTDDINQAQLQ 247 Query: 253 ENADNLLKLHNREKTSL-SVQAIGDLRVRAGNLIYV-LLDALDTRLFLVEQCSHKISGGE 310 + AD L K L ++QA+G V++G +Y+ + +A + + V+ H+ G + Sbjct: 248 QRADVRLAEKKGVKKELKNIQAVGIPEVQSGLPVYISIPEAGIKKTYWVDTDRHEFKGTK 307 Query: 311 HTMSLDI 317 HTM++D+ Sbjct: 308 HTMTIDV 314 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 479,583,467 Number of extensions: 20784321 Number of successful extensions: 52020 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 52001 Number of HSP's successfully gapped: 7 Length of query: 320 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 195 Effective length of database: 417,850,203 Effective search space: 81480789585 Effective search space used: 81480789585 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)