BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0033.p (642 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00014589.1| COG0367: Asparagine synthase (glutamine-hydro... 210 1e-52 ref|NP_105950.1| similar to asparagine synthetase [Mesorhizobium... 200 7e-50 ref|ZP_00014558.1| COG0367: Asparagine synthase (glutamine-hydro... 137 9e-31 ref|ZP_00095578.1| COG0367: Asparagine synthase (glutamine-hydro... 129 2e-28 ref|NP_640415.1| asparagine synthetase like protein [Xanthomonas... 128 5e-28 >ref|ZP_00014589.1| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Rhodospirillum rubrum] Length = 653 Score = 210 bits (534), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 183/627 (29%), Positives = 280/627 (44%), Gaps = 43/627 (6%) Query: 1 MSAIAGIYHLQHQPLDPKQGPDLMQQLSRY-PADE-IRTWQSTDVFLGCLSQWVTPQSLG 58 M+AIAG++ ++ P + L+ Y PA I + + L C +T + Sbjct: 1 MTAIAGLWQDDGVVHTAERVPRMGAALAAYGPARAGISDIAAAGLALTCRQSHITDTDMI 60 Query: 59 ETLPYYDSMRKLAITADAIIDNREELFERL--QVPRGDRVSMPDSLLILLAYEKWGEQAP 116 + P +D R++ + ADA IDN EL V G+R S D+ LIL AY +WG Sbjct: 61 DRQPLWDGDRRICVVADARIDNAAELALGFGWAVGEGERCS--DAALILAAYRRWGPDCV 118 Query: 117 VYLVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLN 176 L GDFAF +WD++++ L ARD G R L+F GL +F ++ L ++ L+ Sbjct: 119 GRLTGDFAFALWDDQEQRLLLARDHYGGRPLFFAHRPGLFAFASMPKGLFALGEFSNALD 178 Query: 177 ERWASEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLR 236 ++A+ + + T+Y+ + ++PP H + V G +Y Q R Sbjct: 179 ATELGAYVALLPQ----EGTRTLYRDLSRVPPGHYLLVQGERRRLVQYWPGQFREPARSG 234 Query: 237 SDQEYEEAFREVFGQAVRD--RLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQPLHTF 294 +E+ E F ++ QAVR R+R +G++ AA EL Q + L F Sbjct: 235 DYREHVEEFGRLYTQAVRACLRVRPGGGIGSHLSAGFDSASVTALAARELSAQAKGLTAF 294 Query: 295 SSYPVDNFVDFNIGKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDEWLDIMEMP 354 + P D F R+ DE E NI+ ++ P +++ L +E P Sbjct: 295 TGAPRDGFTGPYPSARVIDESGVAGEVARRFSNIEHRIIHAGPDGPLRDLERTLPYLETP 354 Query: 355 YKFIENSYWLKGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYREN 414 + I N W + I E A I VLL G GN T S+ + + A+L R+ RW Sbjct: 355 FLNICNLGWARVIEETAARSGIKVLLIGAMGNLTASFEGQI-HLARLFRQGRWWQLAILA 413 Query: 415 RKYNESMGANRRKVFQIVGKKAFPSLTGLLPAKHEAPPDL-----IHPDFARRTDVDERL 469 R GA R V P L +H P + + P FA D+D Sbjct: 414 RGLTRE-GAGWRTVASAALGPYLPDWVRRL--RHRNSPGVFAHTALSPAFAASMDMDGLA 470 Query: 470 KALG-----INSGSSQTIYDIRRKHFEQPHVWNVNGTIATKLSLKYRVWDRDPTNDLRVI 524 A G + + ++ IR P V++ + ++A ++ + RDPT D R++ Sbjct: 471 AARGSFPPRFSDSRTARLFMIR---LADPGVYH-HASLA-----EHGIDIRDPTADRRLL 521 Query: 525 RFCLSVPESQYVQQGVDRSLIRRAMKGLLPDEVRLNRKRRGVQGADGIYRMLPKWDAFIQ 584 FCL++PE +++ G SL R AM GLLPD + LNR+ RGVQ AD +M+ DA Sbjct: 522 DFCLTIPEGFFIRDGRRASLFRDAMAGLLPDTM-LNRRIRGVQAADWYEKMVASRDAMAA 580 Query: 585 ELREMIQDPRTSAYLNNTGLAGCLDRL 611 EL + P S L+ LDRL Sbjct: 581 ELERLRSSPMASRVLD-------LDRL 600 >ref|NP_105950.1| similar to asparagine synthetase [Mesorhizobium loti MAFF303099] dbj|BAB51736.1| similar to asparagine synthetase [Mesorhizobium loti] Length = 639 Score = 200 bits (509), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 188/652 (28%), Positives = 293/652 (44%), Gaps = 81/652 (12%) Query: 1 MSAIAGIYHLQHQPLDPKQGPDLMQQLSRY---PADEIRTWQSTDVFLGCLSQWVTPQSL 57 MS +AGI Q P D D+ + L+R D W + + LG W+ Sbjct: 1 MSGVAGILLRQEAPADAAT--DIQRMLARMRHRGPDGNSWWLDSGIALG--HAWLNTTDE 56 Query: 58 GETLPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPV 117 P + KLAITAD +DNR+EL RL + DR S+ D++L++ AY +WGE PV Sbjct: 57 AGPGPLTMAGGKLAITADCRLDNRDELMARLGIR--DR-SVADAVLLMRAYLRWGESCPV 113 Query: 118 YLVGDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNE 177 +L GDFAF IWD ++ LF ARD G + Y+ + +F + I P+L + G ++E Sbjct: 114 HLQGDFAFAIWDAERQLLFCARDHFGVKPFYYHAADRRFAFASEIGPMLGLDGVGARISE 173 Query: 178 RWASEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELR-LR 236 S FLA D ST Y I LP HS++V+ ++ RY ++ P +R R Sbjct: 174 HRISGFLA----GLPDDPQSTPYSDIFTLPARHSLTVTAQQVVLRRYWQIE--PSVRPAR 227 Query: 237 SDQEYEEAFREVFGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQP-LHTFS 295 SD E F +F Q+VR+R+R R+VGA A + + +P L TFS Sbjct: 228 SDA--AEEFAHLFSQSVRNRMRGSRSVGAMLSGGLDSSSIACVAGLQNAAERKPKLPTFS 285 Query: 296 SYPVDNFVDFNIGKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDEWLDIMEMPY 355 + F G + DER +I +D P + + +P++E + L+ E Sbjct: 286 -------LVFEKGSSM-DERAFIDAVLDQ-HKTDPTLIPVGNYAPFAEFERVLE--EQEG 334 Query: 356 KFIENSYWL-KGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYREN 414 F+ L +GI+ A K I VLL G G+ +S G + +L RW+ +RE Sbjct: 335 TFLAPGLTLTRGIYRTAGAKGIKVLLDGHGGDEVVSQGHG--HLHELANGGRWLDLWREV 392 Query: 415 RKYNESMGANRRKVFQIVGKKAFPSLTGLLPAKHEAPP---------------------- 452 R + + G + ++ F LT PA A Sbjct: 393 RSASNTYGDSTLGLY-------FQFLTLYGPAWRIAKARQLANRALNRIRKPTQMQRGRS 445 Query: 453 --DLIHPDFARRTDVDERLKALGINSGSSQTIYDIRRKHFEQ----PHVWNVNGTIATKL 506 DLI+PD ARRT++ +R G Q + + PH + V A Sbjct: 446 WRDLINPDLARRTELIDRFHRAGYMPPGVQASDALSHRWILSNGYVPHSFEVLDKAAANF 505 Query: 507 SL--KYRVWDRDPTNDLRVIRFCLSVPESQYVQQGVDRSLIRRAMKGLLPDEVRLNRKRR 564 + +Y WD+ ++ FCL++P + + G R ++RRAM+G+LP V+ R + Sbjct: 506 GVEPRYPFWDKP------LVEFCLALPGEEKLSHGFGRYVLRRAMEGILPAAVQWRRDKI 559 Query: 565 GVQGADGIYRMLPKWDAFIQELREMIQDP-RTSAYLNNTGLAGCLDRLGSEP 615 A+ + M+ +++L ++ D R + Y+N ++ RL S+P Sbjct: 560 DFT-ANLVNGMVRNHRDLLEQL--LVSDAGRIAPYVNLPQVSAAYARLLSQP 608 >ref|ZP_00014558.1| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Rhodospirillum rubrum] Length = 644 Score = 137 bits (345), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 145/602 (24%), Positives = 244/602 (40%), Gaps = 33/602 (5%) Query: 1 MSAIAGIYHLQHQPLDPKQGPDLMQQLSRYPADEIRTWQSTDVFLGCLSQWVTPQSLGET 60 M+A G+ + +P D + + + P + +S +TP+ + Sbjct: 1 MAAFCGVVRMDDEPADADEAASMAGAFAAAPGGRPTLRRDRAGAFAAVSLPLTPEDRFDR 60 Query: 61 LPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVYLV 120 P+ ++ + D +D+R L +RL + M D L+ AYE+WG L Sbjct: 61 QPWTGRDGRITLLFDGRLDDRAGLIDRLGLDGKRAEGMADGALVAAAYERWGTAILAGLA 120 Query: 121 GDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNERWA 180 G + +WD R L ARD G R+L++ R G +++F LL +P +R L+E Sbjct: 121 GFWVLAVWDAAARRLVLARDPMGGRSLFYHRQGPVITFAASTLALLRLPRVERVLDETVL 180 Query: 181 SEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLRSDQE 240 + L + + ++ T Y+ I ++P +H + + + + ELRL D++ Sbjct: 181 GDLLML----TMGERDRTFYRDISRVPSAHLVVCTPEGTVIDPFWHPPVGGELRLPRDED 236 Query: 241 YEEAFREVFGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGELRRQGQPLHTFSSYPVD 300 Y EA RE+ +AVR LR+ R V AA L +PL + P Sbjct: 237 YVEAARELLERAVRANLRSLRPVAVLGSGGLDSAGVAATAARLL--APRPLPLYCRVPPT 294 Query: 301 NFVDFNIGKRIADERPYIQETIDYVGNIQPNF--LNFPDLSPYSEIDEWLDIME---MPY 355 ++ + DERP + + + PN ++ P+ ++ + + + Sbjct: 295 DWSGPELSTSYPDERPKLAA----LAALHPNLRVMSVEQAGPHRFDEDQTCLFQRYGLAA 350 Query: 356 KFIENSYWLKGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYRENR 415 + +N WL G +QA+ VLL+G GN T+S P L+R+ R ++ RE Sbjct: 351 RAADNIGWLLGAVDQARAAGHRVLLTGDYGNTTLS-APGRGAPMALLRQGRLMALARELA 409 Query: 416 KYNESMGANRRKV------FQIVGKKAFPSLTGLLPAKHEAPPDLIHPDFARRTDVDERL 469 + G + KV ++I G SL G P + L P+ R ++ R Sbjct: 410 GWRRQRGWSWPKVARETLLYRIPGLSPLRSL-GRNPGSWRS-WTLTRPEAVRALGLEARA 467 Query: 470 KALGINSGSSQTIYDIRRKHFEQPHVWNVNGTIATKLSLKYRVWDRDPTNDLRVIRFCLS 529 +A G T + + G +A + + R P L ++ F L Sbjct: 468 RAAGAPGFPMPTTTPAASRALILERYGRLRGDLAAQTTAVDGFELRSPLAYLPLVEFTLR 527 Query: 530 VPESQYVQQGVDRSLIRRAMKGLLPDEVRLNRKRRGVQGADGIYRMLPKWDAFIQELREM 589 VP Q+ + G R L RR + LP E+ L R G Q P+W + LRE Sbjct: 528 VPSPQFCRDGRLRWLARRTLADRLPPEI-LMEDRLGAQH--------PEWFDTLSSLRES 578 Query: 590 IQ 591 Q Sbjct: 579 YQ 580 >ref|ZP_00095578.1| COG0367: Asparagine synthase (glutamine-hydrolyzing) [Novosphingobium aromaticivorans] Length = 623 Score = 129 bits (325), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 148/592 (25%), Positives = 239/592 (40%), Gaps = 61/592 (10%) Query: 78 IDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVYLVGDFAFMIWDERKRALFG 137 I NR +L R + +G M D+ L + GE A +VGD+A + W ++R+L Sbjct: 59 IFNRTDLLRRFDLKQG----MDDADLYAAVSARMGEAAYRAIVGDYAALQWFPQRRSLRL 114 Query: 138 ARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNERWASEFLAIHTRTDVSDQYS 197 AR + N L+ R G + ++ P+L+ R ++R A E L D + Sbjct: 115 ARSGTSNMPLHVWRDGSRIVISSLPGPILASGVPARINDDRVADELLLFSP-----DGRT 169 Query: 198 TVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPELRLRSDQEYEEAFREVFGQAVRDRL 257 + Y + ++P R +++ PE+RLR D++Y EA +F +AV + Sbjct: 170 SFYAGMHRVPGGGEEVHDPSGFKARRLWSVKDAPEIRLRRDEDYVEALDVLFDEAVSATM 229 Query: 258 RTFRAVGANXXXXXXXXXXXXFAAGELRRQGQPLHTFSSYPVDNFVDFNIGKRIADERPY 317 A G +A L G+ L F+S P+ + DE + Sbjct: 230 DGSSAPGILLSGGFDSQTVASYAVKRLP-AGRRLQAFTSVPMQGWTPPPRPGIFGDESAH 288 Query: 318 IQETIDYVGNIQPNFLNFPDLSPYSE-------IDEWLDIMEMPYKFIENSYWLKGIFEQ 370 ++ I+P+F+ D Y E + W EM N++W EQ Sbjct: 289 VRALCAMYPQIEPHFIEAADFR-YGERGHRQFLVGGWPVFNEM------NAHWSHASLEQ 341 Query: 371 AQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWISFYRENRKYNESMGANRRKVFQ 430 A ++L+G GN S+ Y L RE +W RE K ++ R+ Sbjct: 342 AADLGCDLMLTGDLGNSGFSYDGLTGYPTWL-REGQWFRLLREIGKSDDPRPFWRK---- 396 Query: 431 IVGKKAFPSLTGLLPAKHEAPPDLIHP--------------DFARRTDVDERLKALGINS 476 ++ P + P + + D P DFAR + V +R +A Sbjct: 397 LISLAVMPHV----PVRWKRKMDRHRPWRPSAFATWCGMREDFARESGVLDRAEA----G 448 Query: 477 GSSQTIYDIRRKHFEQPHVWNVNGTIATKLSLKYR----VWDRDPTNDLRVIRFCLSVPE 532 G YDI + +V + A ++ L Y+ V RDPT L ++ FC +P+ Sbjct: 449 GHDIDFYDIPSARTWRANVVDSLNDGAPEIYLGYQLLYGVPMRDPTAYLPLLEFCAGIPD 508 Query: 533 SQYVQQGVDRSLIRRAMKGLLPDEVRLNRKRRGVQGADGIYRMLPKWDAFIQELREMIQD 592 QY++ G R L RR M+G +P+ VRL R G Q +D R + I E+ M D Sbjct: 509 EQYLRDGQTRWLARRLMQGRVPEMVRLE-NRIGAQSSDWPQRFARDREDLIAEMERMKAD 567 Query: 593 PRTSAYLNNTGLAGCLDRLGSEPAPNMIFNSDFRL---LTRGLVFYRFLKRL 641 R + + LA L P S+F++ + R +V RF+ + Sbjct: 568 ERLAKIFDFERLAANLREWDGSDRPES--RSNFKINAAVGRAMVSARFVSHV 617 >ref|NP_640415.1| asparagine synthetase like protein [Xanthomonas axonopodis pv. citri str. 306] gb|AAM34951.1| asparagine synthetase like protein [Xanthomonas axonopodis pv. citri str. 306] Length = 641 Score = 128 bits (321), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 169/631 (26%), Positives = 267/631 (42%), Gaps = 50/631 (7%) Query: 1 MSAIAGIYHLQHQPLDPKQGPDLMQQLSRYPADEIRTWQSTDVFLGCLSQWVTPQSLGET 60 MS AG++ L P+ P L Q L +R WQ V + TPQ E Sbjct: 1 MSDFAGVWRLDGAPVAPGDIQRLGQALDARGIGPVRVWQDGPVAIVHRQHVFTPQDADEA 60 Query: 61 LPYYDSMRKLAITADAIIDNREELFERLQVPRGDRVSMPDSLLILLAYEKWGEQAPVYLV 120 +P+ + + + AD + R+EL LQ P V++PD +L+L A + W A L Sbjct: 61 MPWVGASGAV-LAADVRLAARQELASALQHPAN--VTLPDGVLLLEALQCWDTAALPRLH 117 Query: 121 GDFAFMIWDERKRALFGARDFSGNRTLYFLRSGGLLSFCTVIHPLLSMPGADRTLNERWA 180 G +A ++ + L ARD G R+L+ R L++F T + LL++P + L+ER Sbjct: 118 GCYALALYRPDRHRLLLARDPIGLRSLFVHRGPHLIAFATRLRALLALPQVPKDLDERAL 177 Query: 181 SEFLAIHTRTDVSDQYSTVYQSIEQLPPSHSISVSGGNILFSRYCTLQAPPE---LRLRS 237 ++ L + D + T+Y++I+++P +H++ ++ R+ + PPE LRLR+ Sbjct: 178 ADKLIM----DRARPERTIYRAIDRVPMAHAVVLTPEATHCQRWWS---PPEAGSLRLRN 230 Query: 238 DQEYEEAFREVFGQAVRDRLRTFRAVGANXXXXXXXXXXXXFAAGE------LRRQGQPL 291 D E E A EV +AV D LR V AA + L QPL Sbjct: 231 DAEVEAAAAEVLDRAVADALRARGPVATCLTGGLDSGSITLSAARQQAPSPLLVLTRQPL 290 Query: 292 HTFSSYPVDNFVDFNI-GKRIADERPYIQETIDYVGNIQPNFLNFPDLSPYSEIDE--WL 348 ++ ++ D + +R+A P I + VG+ D + + E D W Sbjct: 291 PATAAATATHYYDESPRARRLAASHPGIDWHL--VGD---------DDADWGEHDPACWW 339 Query: 349 DIMEMPYKFIENSYWLKGIFEQAQMKNIGVLLSGQRGNWTISWGPALDYQAKLIREFRWI 408 P + N W ++ + V+L G+ GN S+ L +L R+ +W Sbjct: 340 REGAQPVRSPSNMAWFFPLYRFLHARGSQVMLGGELGNAFFSYDGLLRL-PQLARQRQWR 398 Query: 409 SFYRENRKYNESMGANRRKVFQIVGKKAFPSLTGLLPAKHEAPPDLIHPDFARRTDVDER 468 + S+G +RRK Q + F + LL H P + A+ Sbjct: 399 VLAGQVGALAGSLGLSRRKTVQRYLLRPFAPVA-LLRLWHGLPAAVWE---AQAALQPAL 454 Query: 469 LKALGINSGSSQTIYDIR--RKHFEQPHV--WNVNGTIATKLSLKYR----VWDRDPTND 520 ++LG+ ++ Y +R H P W + L R V R P D Sbjct: 455 AQSLGMQQSLDRSRYRLRLGGGHTSLPTFREWMLGDAGPMDLVNTLRAVSGVDTRYPLAD 514 Query: 521 LRVIRFCLSVPESQYVQQGVDRSLIRR--AMKGLLPDEVRLNRKRRGVQGADGIYRMLPK 578 RVI F S+P Q+++ GV RSL RR A +G P E+ NR GVQ D R+ + Sbjct: 515 RRVIEFFGSLPLDQFLRDGVSRSLPRRLLAARG-APPEIHANRA-VGVQQGDWFARLNAQ 572 Query: 579 WDAFIQELREMIQDPRTSAYLNNTGLAGCLD 609 DA +L + P + ++ L LD Sbjct: 573 RDALHAQLSQARTSPLANRVVDLQRLQALLD 603 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 964,763,848 Number of extensions: 40476874 Number of successful extensions: 83282 Number of sequences better than 1.0e-05: 110 Number of HSP's gapped: 83320 Number of HSP's successfully gapped: 111 Length of query: 642 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 510 Effective length of database: 398,844,510 Effective search space: 203410700100 Effective search space used: 203410700100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)