BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0024.p (411 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098466.1| COG0477: Permeases of the major facilitator s... 493 e-138 ref|NP_834739.1| Macrolide-efflux protein [Bacillus cereus ATCC ... 335 9e-91 ref|NP_932035.1| hypothetical protein [Photorhabdus luminescens ... 280 5e-74 gb|AAQ73344.1| MefCD [Clostridium difficile] 237 5e-61 ref|ZP_00103736.1| COG0477: Permeases of the major facilitator s... 180 5e-44 >ref|ZP_00098466.1| COG0477: Permeases of the major facilitator superfamily [Desulfitobacterium hafniense] Length = 415 Score = 493 bits (1268), Expect = e-138, Method: Compositional matrix adjust. Identities = 241/399 (60%), Positives = 291/399 (72%) Query: 4 NWKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAG 63 WKKN +LFLTSQT+SLFGSSLVQYAI+WYITL TQSG+MMTISIICGF+PTF LSPFAG Sbjct: 8 EWKKNTILFLTSQTISLFGSSLVQYAIMWYITLETQSGIMMTISIICGFLPTFFLSPFAG 67 Query: 64 VWADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGA 123 VWADRY+RK LIIL+DS I GY MWLLFV+SA+RALG AVQ PAVGA Sbjct: 68 VWADRYNRKRLIILADSMIAISTLILALLFFAGYKMMWLLFVMSAIRALGAAVQMPAVGA 127 Query: 124 ILPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXX 183 +PQLVPE+ L +VNG NGSIQ+ +ML+SPMLSGALLT +IE IFFIDVIT Sbjct: 128 FIPQLVPEDKLLRVNGINGSIQSAVMLVSPMLSGALLTFTTIEAIFFIDVITAALGVSVL 187 Query: 184 XXFVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXX 243 F+ VP H KA QT+SYF DM G YI++H +++ Sbjct: 188 LFFLHVPAHRKAQEAQTVSYFADMSQGLKYIREHAFLKSFFVFCALFMIMAAPAAFLTPL 247 Query: 244 QVTRTFGDDIWRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFTFALGV 303 QV RTFGDD+WRLTAIE+ FS A W GF+NR+++M + +L GV T ALGV Sbjct: 248 QVARTFGDDVWRLTAIEMTFSVGMMIGGILMATWGGFKNRIHSMALGTLGFGVCTVALGV 307 Query: 304 IPVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLIF 363 IPVFW+YL+FM VG++MP FNTP+TV+LQEKVEPD+LGRVFG+L MISS+MMPLGMLIF Sbjct: 308 IPVFWLYLVFMAFVGVSMPIFNTPATVMLQEKVEPDFLGRVFGILSMISSTMMPLGMLIF 367 Query: 364 GPLADVLKIEWMLMLTGLLMFIQGFFLLGSKVLVKAGEP 402 GPLAD + IE +L+ TG++M ++ FL G+KVL++AG P Sbjct: 368 GPLADSIAIEVLLIGTGVIMVLESVFLFGNKVLLEAGRP 406 >ref|NP_834739.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579] gb|AAP11940.1| Macrolide-efflux protein [Bacillus cereus ATCC 14579] Length = 400 Score = 335 bits (860), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 180/392 (45%), Positives = 247/392 (63%), Gaps = 2/392 (0%) Query: 3 SNWKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFA 62 +NWK I+LFLT+QT+SL GSSLVQYAI+WYITL T SG+M+TIS +CGF+P +S FA Sbjct: 7 NNWKTKIILFLTAQTISLLGSSLVQYAIIWYITLTTSSGVMLTISTVCGFLPQIAISLFA 66 Query: 63 GVWADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVG 122 GVW DRY RK +I+LSD I GY +WLLF+V +R+ GT VQTPAV Sbjct: 67 GVWIDRYDRKKMIMLSDGIIAIATLVLAILFLTGYKSIWLLFIVLLIRSAGTGVQTPAVN 126 Query: 123 AILPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXX 182 A++PQ+VP+E+L KVNG N SI +LIM LSP S A+L++ASIE FFIDVIT Sbjct: 127 ALIPQIVPKEYLMKVNGINSSITSLIMFLSPAASAAILSIASIETTFFIDVIT-AMIGIS 185 Query: 183 XXXFVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXX 242 ++V H A +Q S ++ GFSY+K++ +I+ Sbjct: 186 IMLIIQVEGHVNAENKQK-SNLQGIKEGFSYLKENVFIKRLIVLLIIIMILISPAAFLTP 244 Query: 243 XQVTRTFGDDIWRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFTFALG 302 VTR+FG ++WRLTA E+ FS A W GFRNR++T ++ G+ LG Sbjct: 245 LMVTRSFGAEMWRLTASEMTFSAGAAAGGVLIAAWGGFRNRMHTTALACALYGLLMIGLG 304 Query: 303 VIPVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLI 362 + P+F +YL+F L+G+ MP FNTP TVLLQEKVEP GR+F ++ + +S +PLGM+I Sbjct: 305 IAPIFIMYLVFNFLIGITMPCFNTPVTVLLQEKVEPSMHGRMFSLVQIANSCALPLGMII 364 Query: 363 FGPLADVLKIEWMLMLTGLLMFIQGFFLLGSK 394 FGPL+D+ +E +L+ G+ + +++ SK Sbjct: 365 FGPLSDMFSVEILLIYAGIFVLFCAMYIVLSK 396 >ref|NP_932035.1| hypothetical protein [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE17254.1| unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1] Length = 405 Score = 280 bits (716), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 153/401 (38%), Positives = 228/401 (56%), Gaps = 5/401 (1%) Query: 5 WKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFAGV 64 WK+ LFL++QT+SL GSS+VQ++I+WYITL+T SG M+ IS++C F+P L+S FAGV Sbjct: 8 WKRKTALFLSAQTISLLGSSIVQFSIVWYITLSTSSGEMLAISVLCAFLPQVLISLFAGV 67 Query: 65 WADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVGAI 124 WAD Y+RK L I SD+ I +G+ + LLFV VR+ GT +Q PAV AI Sbjct: 68 WADTYNRKWLSIASDALIALVTLGLAISFMLGFKSIELLFVALIVRSFGTGIQMPAVNAI 127 Query: 125 LPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXXXX 184 +PQLVP+E L +VNG N S+ +L+ML SP +SG L + SIE IF +DV T Sbjct: 128 IPQLVPKEKLLRVNGINTSLTSLMMLASPAISGFLYSQTSIENIFLVDVTTAIIGISIVA 187 Query: 185 XFVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXXXXQ 244 +P+ +G + F + GF Y++++ IR Sbjct: 188 F---IPIPKIVSGSMPDNKFQAILAGFRYLQENKLIRNLLIFLICVCFLVSPAAFLTPLL 244 Query: 245 VTRTFGDDIWRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFTFALGVI 304 V RTFG++ WRL E+ FS + W ++N++ ++ G+F ALG Sbjct: 245 VNRTFGEEPWRLAINEMTFSAGAVLGGLLISFWSEYKNKMRITAVACALFGIFMIALGNA 304 Query: 305 PVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLGMLIFG 364 F YL+ +G+ P FN P T + QE+VEP+ +GRVF ++ + S +PLGM++FG Sbjct: 305 YWFPFYLVLNIFLGMVTPCFNAPITAVFQERVEPEMMGRVFSLVQIAMSCSLPLGMVLFG 364 Query: 365 PLADVLKIEWMLMLTGLLMFIQG--FFLLGSKVLVKAGEPV 403 P+AD + I+ +LM++G L+ + G FF+ +V ++ V Sbjct: 365 PIADYISIQSLLMISGGLILLTGTLFFMFNGEVSRESSSAV 405 >gb|AAQ73344.1| MefCD [Clostridium difficile] Length = 416 Score = 237 bits (604), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 136/373 (36%), Positives = 203/373 (54%), Gaps = 5/373 (1%) Query: 3 SNWKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFA 62 S WKK ++LFL SQ +SLFGSSLVQ A++WY+TL T SG+ +T+ +C F+P ++S FA Sbjct: 8 STWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTSSGVWVTVLTVCSFIPQMIISFFA 67 Query: 63 GVWADRYSRKLLIILSDSCIXXXXXXXXXXXXMGY---DEMWLLFVVSAVRALGTAVQTP 119 GVWAD Y+RK+LII+SDS I E+ + V+S +R+LG+ VQTP Sbjct: 68 GVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSNGTTSELIAISVISVIRSLGSGVQTP 127 Query: 120 AVGAILPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVAS-IEVIFFIDVITXXX 178 AV A++PQLVPEEHL + NG N S+Q+++ + P+++ A+L + I + IDV T Sbjct: 128 AVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIVPIVAAAILGFGNKINQVLLIDVFT-AI 186 Query: 179 XXXXXXXFVKVPLHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXX 238 F+K+P H + +S+F DM G Y +IR Sbjct: 187 IAIVLLIFIKIPKHDIPKQTEKVSFFADMVSGIKYCFSDKFIRKVLFTYGAFMLLCVPSG 246 Query: 239 XXXXXQVTRTFGDDIWRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFT 298 + RTFG L+ IE+ + GF+NR T ++ L+ +F+ Sbjct: 247 FLIGLLIERTFGKGYIYLSIIEMIGFLGMVLGGLIIGVFGGFKNRNKTFFLAILSYAIFS 306 Query: 299 FALGVIPVFWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPL 358 LG++ W + +FM ++P + +LQE V P LGRV + +I + +PL Sbjct: 307 IILGLVTQAWQFFIFMFFTNFSIPIIQASAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPL 366 Query: 359 GMLIFGPLADVLK 371 GM+IFGP+ADV+ Sbjct: 367 GMMIFGPMADVIS 379 >ref|ZP_00103736.1| COG0477: Permeases of the major facilitator superfamily [Desulfitobacterium hafniense] Length = 411 Score = 180 bits (457), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 118/384 (30%), Positives = 194/384 (50%), Gaps = 5/384 (1%) Query: 3 SNWKKNIVLFLTSQTVSLFGSSLVQYAILWYITLNTQSGLMMTISIICGFVPTFLLSPFA 62 +W+++ Q VSL S+++Q AI+W++T T S ++++I+ + GF+P +L PF Sbjct: 6 EHWQRSFYTLWAGQAVSLITSAVLQMAIIWHLTEETGSAMVLSIATLVGFLPQAILGPFI 65 Query: 63 GVWADRYSRKLLIILSDSCIXXXXXXXXXXXXMGYDEMWLLFVVSAVRALGTAVQTPAVG 122 GV DRY RK ++I +D I +WL+ VV +R++GTA +PA+ Sbjct: 66 GVLVDRYHRKYVMIGADLLIAAVGLMLALASLAMELPVWLIMVVLFLRSVGTAFHSPALN 125 Query: 123 AILPQLVPEEHLTKVNGTNGSIQALIMLLSPMLSGALLTVASIEVIFFIDVITXXXXXXX 182 A+ P LVPE+ LTK G SIQ+ +L P L+ L V + I +DV Sbjct: 126 AVTPLLVPEDQLTKCAGYTQSIQSASYILGPALAALLYAVWDLNSIILVDV-AGAVLASI 184 Query: 183 XXXFVKVP-LHAKAAGEQTLSYFNDMRLGFSYIKQHNYIRXXXXXXXXXXXXXXXXXXXX 241 FV +P L A + Q + +M+ G+S ++ + + Sbjct: 185 LTAFVFIPRLEAGESAAQG-NLMLEMKEGYSALRSNKGLFALLWVGALYMLFFMPINALF 243 Query: 242 XXQVTRTFGDDIWRLTAIEIAFSXXXXXXXXXXAGWQGFRNRVYTMTMSSLAIGVFTFAL 301 + FG + + +EI F+ W GF+NRV ++ S L +GV Sbjct: 244 PLMSMKYFGGTTFHASLVEIVFAAGMLVGGLVLGVWGGFKNRVMSLAASILLMGVSLMVS 303 Query: 302 GVIPV--FWIYLLFMGLVGLAMPFFNTPSTVLLQEKVEPDYLGRVFGVLGMISSSMMPLG 359 GV+PV + +++L L+G + PF++ T L QEK+ P+YLGRVF +LG + S MPLG Sbjct: 304 GVLPVKGYAVFVLCSTLMGFSAPFYSGVQTALFQEKISPEYLGRVFALLGSVVSVAMPLG 363 Query: 360 MLIFGPLADVLKIEWMLMLTGLLM 383 +++ AD L + +++G+L+ Sbjct: 364 LVLSALFADTLGVNRWFLISGILI 387 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.141 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 502,841,498 Number of extensions: 17755493 Number of successful extensions: 66945 Number of sequences better than 1.0e-05: 133 Number of HSP's gapped: 67039 Number of HSP's successfully gapped: 139 Length of query: 411 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 283 Effective length of database: 409,704,906 Effective search space: 115946488398 Effective search space used: 115946488398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)