BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0019.p (543 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_390834.1| similar to acetate-CoA ligase [Bacillus subtili... 635 e-180 ref|NP_466242.1| similar to acetate-CoA ligase [Listeria monocyt... 633 e-180 ref|NP_472196.1| similar to acetate-CoA ligase [Listeria innocua... 628 e-178 ref|NP_647345.1| acetate-CoA ligase [Staphylococcus aureus subsp... 621 e-176 ref|NP_373133.1| acetate-CoA ligase [Staphylococcus aureus subsp... 621 e-176 >ref|NP_390834.1| similar to acetate-CoA ligase [Bacillus subtilis] pir||E69989 acetate-CoA ligase (EC 6.2.1.1) ytcI - Bacillus subtilis gb|AAC00310.1| YtcI [Bacillus subtilis] emb|CAB14934.1| ytcI [Bacillus subtilis subsp. subtilis str. 168] Length = 531 Score = 635 bits (1637), Expect = e-180, Method: Compositional matrix adjust. Identities = 313/528 (59%), Positives = 382/528 (72%), Gaps = 9/528 (1%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPA--DRIALKWLQENGNYEEITYGDLMKQANRLAGGLSG 71 L + IAP YNL +EME A + AL W E+G E +Y LM++ N++ L+ Sbjct: 4 LKREDLIAPVQYNLVNEMEKFSAAGQKTALLWEDESGKQESWSYEKLMEETNKIGAALAD 63 Query: 72 LGLTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVW 131 LG +GD+++VMVPR + AY +YLA LK G+ VIP SEMLRAKDL YR+ H+E + IV+ Sbjct: 64 LGFKKGDKLIVMVPRVLEAYAVYLAILKSGMVVIPCSEMLRAKDLEYRIEHAEVKGAIVY 123 Query: 132 SEVTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAI 191 SE G ++ + L++G E +GW L + E F + RDD+A Sbjct: 124 SEFIGAFRDVSTADKLIT--LSIG----ENDAGWKNLLSI-EADGSQFKTADTTRDDMAF 176 Query: 192 LAYTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTV 251 L+YTSGTTG PK VVHTHGW +AHLK + WLDI E D VWATAAPGWQKW+WSPFL V Sbjct: 177 LSYTSGTTGQPKGVVHTHGWAFAHLKTSAGAWLDISEKDIVWATAAPGWQKWVWSPFLAV 236 Query: 252 LGNGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVS 311 LG+GATGFVY+G F +++L+LL I V CCTPTEYRLMAK EGL R+DLS L AVS Sbjct: 237 LGSGATGFVYHGRFKAEKYLELLNRYKINVFCCTPTEYRLMAKVEGLKRFDLSALHSAVS 296 Query: 312 AGEPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVID 371 AGEPLN+EVI+ F++HF I +RDGYGQTESTL++ LKD PI+ GSMGK VE+I+ Sbjct: 297 AGEPLNREVIDVFQKHFGIKVRDGYGQTESTLLVGVLKDTPIKPGSMGKPTPGNQVEIIN 356 Query: 372 GEGNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFE 431 +G G VGDIAVHLS PALFK Y+KDPER G+YF+TGDRARKDE+GYFWFE Sbjct: 357 EDGEICKPGEVGDIAVHLSTPALFKEYFKDPERMKTQIRGDYFLTGDRARKDEEGYFWFE 416 Query: 432 GRGDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTP 491 R DDIIISSGYTIGPFEVE+AL+KH VKECAVVASPDEIRGS+VKA+VVL++H + Sbjct: 417 SRNDDIIISSGYTIGPFEVEDALVKHPEVKECAVVASPDEIRGSIVKAYVVLQNHEKRSD 476 Query: 492 ELVKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKK 539 ELVK LQ+HVK TAPYKYPR+IEF+ LPKTAS KIRRVELR +E++ Sbjct: 477 ELVKMLQNHVKTITAPYKYPREIEFVESLPKTASAKIRRVELRKREEQ 524 >ref|NP_466242.1| similar to acetate-CoA ligase [Listeria monocytogenes EGD-e] pir||AG1414 acetate-CoA ligase homolog lmo2720 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00933.1| lmo2720 [Listeria monocytogenes] Length = 525 Score = 633 bits (1632), Expect = e-180, Method: Compositional matrix adjust. Identities = 302/524 (57%), Positives = 380/524 (72%), Gaps = 5/524 (0%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLG 73 ++++ IAPE YN+T E+ +++ AL W E+G + +Y L++QAN+ A G Sbjct: 1 MNSENLIAPELYNITDEIAKFSSEKTALIWKNEHGETKTWSYHHLLEQANKFANVAKDAG 60 Query: 74 LTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSE 133 + +GD V+VM PR + Y IY+ K G +IP+SE+L+A DL YR+ H+ +A++ ++ Sbjct: 61 IKKGDHVIVMTPRLLETYAIYMGLWKAGAIIIPASELLKAHDLEYRIHHANVKAIVSYNG 120 Query: 134 VTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILA 193 +T E +KI +P++ ++ VG K SGW E LME P F V + RDD +LA Sbjct: 121 MTAEFDKIES-IPSVSKKIIVGDKL----SGWEQYETLMEAAPTEFERVETSRDDACLLA 175 Query: 194 YTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLG 253 +TSGTTGNPK VVH HGWGYAH++I + WLDI E D VWATA PGWQKW+WSPFL+VLG Sbjct: 176 FTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLG 235 Query: 254 NGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAG 313 GATGF+YNG F P++ L LL+++ I VLCCTPTEYRLMAK L +DLS LR AVSAG Sbjct: 236 KGATGFIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSSLRSAVSAG 295 Query: 314 EPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGE 373 EPLN+EVI FR++FDI +RDGYGQTESTL+I L D PIR GSMGK I P + +ID + Sbjct: 296 EPLNREVIQVFRDNFDIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAD 355 Query: 374 GNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGR 433 GNP+ G +GDIA+ PALFK YYK+PER G+YFV+GDRA +DED Y+WF+GR Sbjct: 356 GNPVGVGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGR 415 Query: 434 GDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPEL 493 DDIIISSGYTIGPFEVE+AL H VKE AVVASPDEIRG+VVKAF+V+KD GT +L Sbjct: 416 NDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVVKDGYKGTDDL 475 Query: 494 VKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQE 537 V ELQ+ KE TAPYKYPR+IEF+ LPKT SGKIRRVELRD E Sbjct: 476 VHELQTFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAE 519 >ref|NP_472196.1| similar to acetate-CoA ligase [Listeria innocua] pir||AF1790 acetate-CoA ligase homolog lin2868 [imported] - Listeria innocua (strain Clip11262) emb|CAC98094.1| lin2868 [Listeria innocua] Length = 525 Score = 628 bits (1620), Expect = e-178, Method: Compositional matrix adjust. Identities = 301/524 (57%), Positives = 381/524 (72%), Gaps = 5/524 (0%) Query: 14 LDNQQWIAPEFYNLTSEMEHHPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLG 73 +++++ IAPE YN+T E+ +++ AL W E+ + +Y L++QAN+ A + G Sbjct: 1 MNSEKLIAPELYNITDEIAKFSSEKTALIWKNEHDETKTWSYRHLLEQANKFANVVKDAG 60 Query: 74 LTQGDRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSE 133 + +GD V+VM PR + Y IY+ K G +IP+SE+L+A DL YR+RH+ +A++ ++ Sbjct: 61 VKKGDHVIVMTPRLLETYAIYMGLWKAGAIIIPASELLKAHDLEYRIRHANVKAIVSYNG 120 Query: 134 VTGEVNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILA 193 +T E +KI ++P++ ++ VG K SGW E LME F V + RDD +LA Sbjct: 121 MTAEFDKIE-NIPSVSKKIIVGEKL----SGWDHYETLMETASTEFERVETSRDDACLLA 175 Query: 194 YTSGTTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLG 253 +TSGTTGNPK VVH HGWGYAH++I + WLDI E D VWATA PGWQKW+WSPFL+VLG Sbjct: 176 FTSGTTGNPKGVVHIHGWGYAHIRIAADHWLDIHEDDIVWATAGPGWQKWVWSPFLSVLG 235 Query: 254 NGATGFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAG 313 GATGF+YNG F P++ L LL+++ I VLCCTPTEYRLMAK L +DLS LR AVSAG Sbjct: 236 KGATGFIYNGRFIPEKQLHLLEEEKINVLCCTPTEYRLMAKVNNLREHDLSALRSAVSAG 295 Query: 314 EPLNQEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGE 373 EPLN+EVI F+++FDI +RDGYGQTESTL+I L D PIR GSMGK I P + +ID E Sbjct: 296 EPLNREVIQVFQDNFDIKVRDGYGQTESTLLIGTLVDTPIRPGSMGKPIMPEYMAIIDAE 355 Query: 374 GNPLPAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGR 433 G P+ G +GDIA+ PALFK YYK+PER G+YFV+GDRA +DED Y+WF+GR Sbjct: 356 GTPVGVGEIGDIAMRRDFPALFKEYYKEPERLQKAIRGDYFVSGDRAIRDEDNYYWFQGR 415 Query: 434 GDDIIISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPEL 493 DDIIISSGYTIGPFEVE+AL H VKE AVVASPDEIRG+VVKAF+VLKD GT +L Sbjct: 416 NDDIIISSGYTIGPFEVEDALTHHPAVKEVAVVASPDEIRGTVVKAFIVLKDGYEGTEDL 475 Query: 494 VKELQSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQE 537 V ELQ+ KE TAPYKYPR+IEF+ LPKT SGKIRRVELRD E Sbjct: 476 VHELQTFTKEQTAPYKYPRRIEFVEALPKTDSGKIRRVELRDAE 519 >ref|NP_647345.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus MW2] dbj|BAB96393.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus MW2] Length = 532 Score = 621 bits (1602), Expect = e-176, Method: Compositional matrix adjust. Identities = 304/526 (57%), Positives = 375/526 (71%), Gaps = 8/526 (1%) Query: 20 IAPEFYNLTSEMEH--HPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLGLTQG 77 APE YN+ SE+E H D+ A+ + G +TY L++Q+N++ L+ GL++G Sbjct: 7 FAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKG 66 Query: 78 DRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSEVTGE 137 D+VL+M+PR I Y +Y+A LKLG+A+IP SEMLR KDL YR+ H E AVI T E Sbjct: 67 DKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVE 126 Query: 138 VNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILAYTSG 197 KI + AL + G K+ GW LE E + RDD+AIL+YTSG Sbjct: 127 FEKIK-EYDALTKFVIAGQKD-----GWISLEAEKENASNKLEMADTTRDDMAILSYTSG 180 Query: 198 TTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLGNGAT 257 TTGNPKAV H HGWG+AHL++ WL I E D VWATAAPGWQKW+WSPFL+VLG+GAT Sbjct: 181 TTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGAT 240 Query: 258 GFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAGEPLN 317 FV+NG F P+ +L+LLQ I VLCCTPTEYR+MAK + L YDL+ L AVSAGEPLN Sbjct: 241 AFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLN 300 Query: 318 QEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGEGNPL 377 +EV+ F+++F++T+RDGYGQTESTL+I LKD P R+GSMGK I V V+D EGN + Sbjct: 301 REVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSV 360 Query: 378 PAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGRGDDI 437 PA G+IAV L +PALFK YYK+PER G+Y++TGD A D+DGYFWFEGR DDI Sbjct: 361 PANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDI 420 Query: 438 IISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPELVKEL 497 IISSGYTIGPFEVE+AL H VKECAVVA P +IRG++VKAFV+L+DH+AG LVKEL Sbjct: 421 IISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKEL 480 Query: 498 QSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAAQE 543 Q VK APYKYPR+IEF+ DLPKT SGKIRRVELRD E + Q+ Sbjct: 481 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDSEIEKWQQ 526 >ref|NP_373133.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus Mu50] ref|NP_375728.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus N315] pir||A99968 acetate-CoA ligase [imported] - Staphylococcus aureus (strain N315) dbj|BAB43707.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus N315] dbj|BAB58771.1| acetate-CoA ligase [Staphylococcus aureus subsp. aureus Mu50] Length = 539 Score = 621 bits (1602), Expect = e-176, Method: Compositional matrix adjust. Identities = 304/526 (57%), Positives = 375/526 (71%), Gaps = 8/526 (1%) Query: 20 IAPEFYNLTSEMEH--HPADRIALKWLQENGNYEEITYGDLMKQANRLAGGLSGLGLTQG 77 APE YN+ SE+E H D+ A+ + G +TY L++Q+N++ L+ GL++G Sbjct: 14 FAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTYQQLIEQSNKVGNVLASHGLSKG 73 Query: 78 DRVLVMVPRRIIAYVIYLACLKLGLAVIPSSEMLRAKDLAYRLRHSEARAVIVWSEVTGE 137 D+VL+M+PR I Y +Y+A LKLG+A+IP SEMLR KDL YR+ H E AVI T E Sbjct: 74 DKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRTKDLQYRITHGEINAVIALEPFTVE 133 Query: 138 VNKITGDLPALDYRLAVGGKEEELGSGWAGLEGLMEGQPESFPAVASRRDDIAILAYTSG 197 KI + AL + G K+ GW LE E + RDD+AIL+YTSG Sbjct: 134 FEKIK-EYDALTKFVIAGQKD-----GWISLEAEKENASNKLEMADTTRDDMAILSYTSG 187 Query: 198 TTGNPKAVVHTHGWGYAHLKIVSSLWLDIRESDTVWATAAPGWQKWIWSPFLTVLGNGAT 257 TTGNPKAV H HGWG+AHL++ WL I E D VWATAAPGWQKW+WSPFL+VLG+GAT Sbjct: 188 TTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWATAAPGWQKWVWSPFLSVLGSGAT 247 Query: 258 GFVYNGPFHPDRFLQLLQDQGIQVLCCTPTEYRLMAKTEGLARYDLSKLRCAVSAGEPLN 317 FV+NG F P+ +L+LLQ I VLCCTPTEYR+MAK + L YDL+ L AVSAGEPLN Sbjct: 248 AFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMAKLQNLNDYDLTHLHSAVSAGEPLN 307 Query: 318 QEVINTFREHFDITIRDGYGQTESTLIIAALKDQPIRVGSMGKSIAPGIVEVIDGEGNPL 377 +EV+ F+++F++T+RDGYGQTESTL+I LKD P R+GSMGK I V V+D EGN + Sbjct: 308 REVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDTPQRIGSMGKGIPGSSVTVVDDEGNSV 367 Query: 378 PAGVVGDIAVHLSMPALFKNYYKDPERKVNCSHGEYFVTGDRARKDEDGYFWFEGRGDDI 437 PA G+IAV L +PALFK YYK+PER G+Y++TGD A D+DGYFWFEGR DDI Sbjct: 368 PANTKGNIAVPLDLPALFKGYYKEPERTAKAQTGKYYITGDLAHVDDDGYFWFEGRRDDI 427 Query: 438 IISSGYTIGPFEVEEALMKHALVKECAVVASPDEIRGSVVKAFVVLKDHSAGTPELVKEL 497 IISSGYTIGPFEVE+AL H VKECAVVA P +IRG++VKAFV+L+DH+AG LVKEL Sbjct: 428 IISSGYTIGPFEVEDALTNHPAVKECAVVAKPHDIRGNIVKAFVILQDHTAGDDTLVKEL 487 Query: 498 QSHVKEWTAPYKYPRKIEFIADLPKTASGKIRRVELRDQEKKAAQE 543 Q VK APYKYPR+IEF+ DLPKT SGKIRRVELRD E + Q+ Sbjct: 488 QQFVKNEVAPYKYPREIEFVDDLPKTNSGKIRRVELRDSEIEKWQQ 533 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 872,666,882 Number of extensions: 38819348 Number of successful extensions: 114736 Number of sequences better than 1.0e-05: 5207 Number of HSP's gapped: 104991 Number of HSP's successfully gapped: 5880 Length of query: 543 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 412 Effective length of database: 401,559,609 Effective search space: 165442558908 Effective search space used: 165442558908 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)