BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0078.0002.p (847 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_465539.1| similar to alpha-mannosidase [Listeria monocyto... 273 1e-71 dbj|BAB40444.2| alpha-D-mannosidase [Bacillus sp. GL1] 273 1e-71 ref|NP_561772.1| alpha-mannosidase [Clostridium perfringens] >gi... 270 1e-70 ref|NP_471457.1| similar to alpha-mannosidase [Listeria innocua]... 269 3e-70 ref|NP_562996.1| alpha-mannosidase [Clostridium perfringens] >gi... 266 1e-69 >ref|NP_465539.1| similar to alpha-mannosidase [Listeria monocytogenes EGD-e] pir||AG1326 alpha-mannosidase homolog lmo2015 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00093.1| lmo2015 [Listeria monocytogenes] Length = 1036 Score = 273 bits (699), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 155/409 (37%), Positives = 226/409 (55%), Gaps = 21/409 (5%) Query: 84 SGGEGLLRVNGESRQGLDRNHTFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQP 143 SG E LL VNGE QG+D+NH S G+ +EL L+ + P Q ++ Sbjct: 101 SGFESLLFVNGEPYQGVDQNHREVLFPDSFAGKTIELVFRLWSGLEGGGTPTIQTHQLK- 159 Query: 144 PVRAIRTMLVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYRVIDGFLNMDET 203 + +N + L +++ L E + +L+A+ R +L +D + Sbjct: 160 -----EAFIGYLNLMIDDLYFTSKATLKTLDQLEEKNPTYAPLLQAINR---AYLAIDWS 211 Query: 204 ---AVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTF 260 +++ ++ + L+A + I N + VG +HID+AWLW + T K +R+F Sbjct: 212 TPGSIQNLISMETANQNLQAALKKIPKNFPIKVAAVGHTHIDVAWLWRLKHTREKAARSF 271 Query: 261 STVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNI 320 STV LME+YPEY F QSQPQL+ Y+K++ P +YAK+K +I EG WE GGMW+E D NI Sbjct: 272 STVLHLMEDYPEYLFLQSQPQLYAYIKEDYPEIYAKIKAKISEGNWEADGGMWLEADCNI 331 Query: 321 PSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWN 380 PSGESL+RQMLYGQ+F EEFGK S WLPD FGY +LPQILR G++ FMTTK+ WN Sbjct: 332 PSGESLVRQMLYGQKFLREEFGKPSSFLWLPDVFGYSWALPQILRKSGIKTFMTTKISWN 391 Query: 381 DTNVFPYDLFHWVGIDGTSMLSYLNHGVNENT---------LPKDVHDHWQSFREKNIHN 431 N P+D F W G+DGT +L++ +N+ +++ W +++K ++ Sbjct: 392 QYNRMPHDTFQWRGMDGTEILTHFITAPEKNSRISTYNGKMTARELVGLWDKYQDKEVNQ 451 Query: 432 EQMLLYXXXXXXXXVTREMLEYIRRAELMVGQPASTFSNAAGFFSGIGE 480 E +L Y V REMLE RR + M G P +A +F+ + E Sbjct: 452 ELLLAYGYGDGGGGVNREMLEMRRRYDAMPGIPEVKPKSATTYFNDLHE 500 >dbj|BAB40444.2| alpha-D-mannosidase [Bacillus sp. GL1] Length = 1047 Score = 273 bits (698), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 174/518 (33%), Positives = 264/518 (50%), Gaps = 48/518 (9%) Query: 1 MERIRRFIRELSERQWLEQQKLEGWDIQ---AGVYTVPGQYDGMRPYTEGGDFTLFPSVQ 57 +E++++ I EL+ ++ L+ W++Q AG V RP G+ + + Sbjct: 5 VEKLQKRIEELAAYRYRHALPLDEWEMQEDEAGANGVS------RPPELTGELRMRKGER 58 Query: 58 GTTY----FFRRTLAVPAEWAGQRAGLIFESG---------GEGLLRVNGESRQGLDRNH 104 Y + RR + VP +W G+ +F+ G E LL +NG QG+D NH Sbjct: 59 WVGYDRYVWLRRKVRVPEDWKGREVVGLFDFGLTGAGNNAAFESLLYLNGRPFQGVDTNH 118 Query: 105 TFAALEPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMY 164 + G +EL L+ + P +Q+ + R L +++ A L + Sbjct: 119 QEVFVPAELAGSEIELVFRLWSGLEGGGGPRDQEHQFK------RAELAVLDAAADDLYF 172 Query: 165 SAVIVRDAAVLLPEADMRRTRMLEAL---YRVIDGFL-NMDETAVREGSAVAALEKKLRA 220 + + A +L E++ + +L A ++++D DE V G A AL R Sbjct: 173 TGRAALETAKILEESNPFKHDLLTAADRAFKLLDWSRPGSDEFYVSVGLAADAL----RT 228 Query: 221 DIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQP 280 ++ + T+ +G +HID+AWLW + T K++R+FSTV LME+YPEY F Q+QP Sbjct: 229 ALSELDNKHPVTVRALGHTHIDVAWLWQLKHTREKSARSFSTVLRLMEKYPEYLFLQTQP 288 Query: 281 QLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEE 340 QL+ YLK + P +Y +++ER++EGRWE G MW+E D N+ SGESL+RQ+L+G RF+ +E Sbjct: 289 QLYAYLKKDYPEIYEQIRERVKEGRWEAGGAMWLEADCNLTSGESLVRQILHGTRFFRDE 348 Query: 341 FGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSM 400 FG D WLPD FGY +LPQILR G++ FMTTK+ WN N P+D F W GIDG+ + Sbjct: 349 FGVECDYLWLPDVFGYSWALPQILRKSGIKTFMTTKISWNQYNRMPHDTFKWRGIDGSEI 408 Query: 401 LSYL------------NHGVNENTLPKDVHDHWQSFREKNIHNEQMLLYXXXXXXXXVTR 448 L++ + N P V W ++R+K+I+ E +L Y V R Sbjct: 409 LTHFITTPEVADPNVWWYTYNGQIEPFTVKGIWDTYRDKDINRELLLSYGYGDGGGGVNR 468 Query: 449 EMLEYIRRAELMVGQPASTFSNAAGFFSGIGERQPQVD 486 MLE RR + M G P A FF + E D Sbjct: 469 HMLEMRRRLDEMPGLPKVVPGRAGDFFDKLHETVAGTD 506 >ref|NP_561772.1| alpha-mannosidase [Clostridium perfringens] dbj|BAB80562.1| alpha-mannosidase [Clostridium perfringens str. 13] Length = 1022 Score = 270 bits (689), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 138/341 (40%), Positives = 198/341 (58%), Gaps = 24/341 (7%) Query: 152 LVLVNEAVQSLMYSAVIVRDAAVLLPEADMRRTRMLEALYR----------------VID 195 + NE V++L Y ++ + LP R +L ALY + D Sbjct: 164 IATCNEEVKALYYDVEVLHELMNQLPGDSARYHTILNALYEASKVLSIKTGMSGCDFLKD 223 Query: 196 GFLNMDETAVREGSAVAALEKKLRADIAGIGGNAEGTIHMVGQSHIDIAWLWPARETVRK 255 G + E + V + L+ ++ GG+ ++ +G +HID+AWLWP RET+RK Sbjct: 224 GVVTQQEVTILNEEEVKKAREILKKELDKKGGDPSLSVSAIGHAHIDLAWLWPIRETIRK 283 Query: 256 TSRTFSTVNALMEEYPEYQFAQSQPQLFQYLKDNDPVLYAKVKERIREGRWELVGGMWVE 315 +RT+STV A ME+Y EY F SQPQL+Q++K+ P LY ++KE+I EGRWE GGMWVE Sbjct: 284 GARTYSTVLANMEKYSEYVFGASQPQLYQWMKEYYPDLYGRIKEKIEEGRWEAQGGMWVE 343 Query: 316 PDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEWLPDTFGYCASLPQILRHGGVRYFMTT 375 PD N+PSGESL+RQ+LYG+R++ EEF K D WLPD FGY A+LPQIL+ GV YFMT Sbjct: 344 PDTNVPSGESLVRQLLYGKRYFREEFKKEMDTLWLPDVFGYSAALPQILKKSGVDYFMTI 403 Query: 376 KLGWNDTNVFPYDLFHWVGIDGTSMLSYL--NHGVNENTLPKDVHDHWQSFREKNIHNEQ 433 KL WN+ N FP+ F W G+DG+ +LS++ N + P+ V ++F +K + +E Sbjct: 404 KLSWNNHNQFPHHTFVWEGLDGSRVLSHMPPEGTYNSSAAPRAVAKSEKAFLDKGLSDEC 463 Query: 434 MLLYXXXXXXXXVTREMLEYIRRAELMVG------QPASTF 468 ++L+ E LE +RR + G +P+S F Sbjct: 464 LMLFGIGDGGGGPGEEHLERLRRERNINGIAPVKQEPSSNF 504 >ref|NP_471457.1| similar to alpha-mannosidase [Listeria innocua] pir||AI1697 alpha-mannosidase homolog lin2123 [imported] - Listeria innocua (strain Clip11262) emb|CAC97353.1| lin2123 [Listeria innocua] Length = 1032 Score = 269 bits (687), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 162/446 (36%), Positives = 233/446 (52%), Gaps = 30/446 (6%) Query: 62 FFRRTLAVPAEWAGQRAGLIFE---------SGGEGLLRVNGESRQGLDRNHTFAALEPS 112 + + T+ P+ G R F+ SG E LL VNGE QG+D+NH + Sbjct: 66 WLKTTITFPSAKEGTRLIGYFDFGNTGDGHNSGFESLLFVNGEPFQGVDQNHQEVLFPDN 125 Query: 113 PDGRPLELEIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMYSAVIVRDA 172 G+ EL L+ + P Q ++ + +N + L +++ Sbjct: 126 FAGKTTELVFRLWSGLEGGGVPSIQTHQLK------EAFIGYLNLVIDDLYFTSKATLKT 179 Query: 173 AVLLPEADMRRTRMLEALYRVIDGFLNMDETAVREGSAVAALEKK---LRADIAGIGGNA 229 L E + +L+A+ R +L +D ++++E L+A I + N Sbjct: 180 LDQLEENNPTHAPLLQAINR---AYLAIDWGTPGSNQNLSSMENANQILQAAIKEMPKNF 236 Query: 230 EGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQPQLFQYLKDN 289 + VG +HID+AWLW + T K +R+FSTV LME+YPEY F QSQPQL+ Y+K++ Sbjct: 237 PIKVAAVGHTHIDVAWLWRLKHTREKAARSFSTVLHLMEDYPEYLFLQSQPQLYAYIKED 296 Query: 290 DPVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDIEW 349 P +YAK+K +I EG WE GGMW+E D NIPSGESL+RQMLYGQ+F EEFGK S W Sbjct: 297 YPEIYAKIKAKIEEGNWEADGGMWLEADCNIPSGESLVRQMLYGQKFLREEFGKPSSFLW 356 Query: 350 LPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSMLSYLNHGVN 409 LPD FGY +LPQIL G++ FMTTK+ WN N P+D F W GIDGT +L++ Sbjct: 357 LPDVFGYSWALPQILCKSGIKTFMTTKISWNQYNRMPHDTFQWRGIDGTEILTHFITAPE 416 Query: 410 ENT---------LPKDVHDHWQSFREKNIHNEQMLLYXXXXXXXXVTREMLEYIRRAELM 460 +N+ +++ W +++K I+ E +L Y V REMLE RR + M Sbjct: 417 KNSRISTYNGKMTARELVGLWDKYQDKEINQELLLAYGYGDGGGGVNREMLEMRRRYDKM 476 Query: 461 VGQPASTFSNAAGFFSGIGERQPQVD 486 G P +A +F+ + E D Sbjct: 477 PGIPEVKPKSATDYFNNLHETIQSTD 502 >ref|NP_562996.1| alpha-mannosidase [Clostridium perfringens] dbj|BAB81786.1| alpha-mannosidase [Clostridium perfringens str. 13] Length = 1044 Score = 266 bits (680), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 171/508 (33%), Positives = 259/508 (50%), Gaps = 44/508 (8%) Query: 2 ERIRRFIRELSERQWLEQQKLEGWDIQAGVYTVPGQYDGMRPYTEGG--DFTLFPSVQGT 59 ERIR+ E+ + ++ + AG Y D ++ E G DF Sbjct: 6 ERIRKTYEEIGRYVYSNTLNIDNYKFIAGKY---DNIDEIKNAPEKGWIDFKTGDLWGEE 62 Query: 60 TY-FFRRTLAVPAEWAGQRAGLIFES---------GGEGLLRVNGESRQGLDRNHTFAAL 109 ++ +F+ ++ VP E+AGQ L F + + +L VNGE QGLD NH L Sbjct: 63 SHGWFKTSVKVPEEFAGQTIALSFHTFLGDWDNTTNPQFILYVNGEHIQGLDVNHQETIL 122 Query: 110 EPSPDGRPLELEIELFDPIPEPVDPLNQQAVIQPPVRAIRTMLVLVNEAVQSLMYSAVIV 169 + + E++D +D I+ + LV ++E + L ++ + Sbjct: 123 THNA------VAGEVYD-----IDLHAHIGRIEGKKSTLHGKLVTIDEEARKLYFNLSVP 171 Query: 170 RDAAVLLPEADMRRTRMLEALYRVIDGFLNMDETAVREGSAVAALEKKLRAD--IAGIGG 227 A L + D RR ML L I+ +++ +E + + K + + G Sbjct: 172 HIVATKLHDEDKRRIDMLTVLNDTIN-LIDLRRPFTKEYNESLKIANKYIEENFYDKMCG 230 Query: 228 NAEGTIHMVGQSHIDIAWLWPARETVRKTSRTFSTVNALMEEYPEYQFAQSQPQLFQYLK 287 + + VG +HID+AWLW +T K +R+FSTV LMEEYPEY F SQPQL+++LK Sbjct: 231 HEDMVATCVGHTHIDVAWLWTVAQTREKVARSFSTVLKLMEEYPEYIFMSSQPQLYKFLK 290 Query: 288 DNDPVLYAKVKERIREGRWELVGGMWVEPDLNIPSGESLMRQMLYGQRFYLEEFGKTSDI 347 ++ P +Y KVKE++REG WE G MW+E D N+ SGESL+RQ+L+G+RF+ EEF ++I Sbjct: 291 EDHPKVYEKVKEKVREGVWEPEGAMWLEADCNVTSGESLIRQILHGKRFFKEEFNVDNEI 350 Query: 348 EWLPDTFGYCASLPQILRHGGVRYFMTTKLGWNDTNVFPYDLFHWVGIDGTSML------ 401 WLPD FGY A+LPQIL+ V YFMTTK+ WN N PYD F W GIDGT +L Sbjct: 351 LWLPDVFGYSAALPQILKKSDVNYFMTTKISWNQINKMPYDTFMWKGIDGTEILTHFITT 410 Query: 402 SYLNHGVNENTL--------PKDVHDHWQSFREKNIHNEQMLLYXXXXXXXXVTREMLEY 453 +Y + + T+ P + W +++KNI+N+ ++ + T EMLE Sbjct: 411 TYPDQDPFKETMTTYCGNIHPGSIMGAWNRYQQKNINNDVLVAFGFGDGGGGATYEMLEN 470 Query: 454 IRR-AELMVGQPASTFSNAAGFFSGIGE 480 +R ++ + G P A +F + E Sbjct: 471 GKRLSKGIPGAPKVKIGKAKDYFKKLEE 498 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,264,745,340 Number of extensions: 54615463 Number of successful extensions: 104357 Number of sequences better than 1.0e-05: 76 Number of HSP's gapped: 104350 Number of HSP's successfully gapped: 76 Length of query: 847 Length of database: 757,237,578 Length adjustment: 134 Effective length of query: 713 Effective length of database: 393,414,312 Effective search space: 280504404456 Effective search space used: 280504404456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)