BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0005.p (749 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_347440.1| Pectate lyase related protein, secreted [Clostr... 554 e-156 gb|AAG59609.1| pectate lyase A [Clostridium cellulovorans] 124 1e-26 pir||JC6502 pectate lyase (EC 4.2.2.2) - Amycolata sp >gi|219670... 114 1e-23 ref|ZP_00067834.1| COG3866: Pectate lyase [Microbulbifer degrada... 106 2e-21 ref|ZP_00067832.1| COG3866: Pectate lyase [Microbulbifer degrada... 106 3e-21 >ref|NP_347440.1| Pectate lyase related protein, secreted [Clostridium acetobutylicum] pir||A96999 pectate lyase related protein, secreted [imported] - Clostridium acetobutylicum gb|AAK78780.1| Pectate lyase related protein, secreted [Clostridium acetobutylicum] Length = 564 Score = 554 bits (1428), Expect = e-156, Method: Compositional matrix adjust. Identities = 282/535 (52%), Positives = 370/535 (69%), Gaps = 20/535 (3%) Query: 51 VYAAGLSITGSGGWNETAYVEWSPVSNVQGYSVYVKPASASDSQYQQLNNELIRRYPSYW 110 V + +SI S G E+A VEWSPV Y+VY K A A DS Y ++++ELIR+YPS + Sbjct: 44 VETSAVSILESEGSLESANVEWSPVQGAASYNVYYKAADALDSSYTKIDDELIRQYPSSF 103 Query: 111 RADAVGLAAGSYVMKVEXXXXXXXXXXXXXXXXXXT--SYDRAGFAFSASSPYGTGSGAY 168 RAD +GLAAG+YV+K+ T + R GFAFS +SP T SG Y Sbjct: 104 RADVLGLAAGNYVLKIVPVVNGVDAINEEATTQALTVKANKREGFAFSPASPMKTSSGGY 163 Query: 169 NENGTLKNGAQVLYITSQNAQTVTLPVKINSSGAVQTGVGLGGILTLRQKGYDTTPLAIR 228 N++GT+ + A++LYIT+ N TVT V N+ G T GL IL RQKGYD +PL IR Sbjct: 164 NDDGTVASNAEILYITASNVNTVTENVVTNAKGTKTTCTGLVNILAGRQKGYDKSPLIIR 223 Query: 229 IIGKVTAADLSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLLRYVGNVEVR 288 +IG++ A+D++G LN Y+++KG N+T EG+G DA AYGWG L+R N+E+R Sbjct: 224 MIGEIKASDITG-LNEHTYIQLKG-----CYNVTFEGVGKDATAYGWGFLIRDARNLELR 277 Query: 289 NLGVMLFPDDGISMDTGNVNVWVHNNDIFYGSAGSDADQAKGDGSTDLKKGSTYITISYN 348 N+G+M+F DD IS+DT N N+WVHNND+FYG+AG DADQ KGDGS D+K+ ST++++SYN Sbjct: 278 NVGIMMFGDDAISLDTDNQNIWVHNNDLFYGAAGHDADQVKGDGSCDIKQDSTFVSVSYN 337 Query: 349 HYWDSGKASLVGLSEPAEFFVTFHHNWFDHSDSRHPRIRVASVHVYNNFYDGVSKYGVGV 408 H+ DSGK+SL G+ + ++VT+HHNWFDHSDSRHPRIRV SVHVYNN++DG +KYGVGV Sbjct: 338 HFHDSGKSSLCGMQDTQNYYVTYHHNWFDHSDSRHPRIRVGSVHVYNNYFDGNAKYGVGV 397 Query: 409 TTGASAFVESNVFRHAKYPMMIALQGTDAL--GEGTFSGENGGMIKAYNNTVVDAASLIY 466 T GASAFVE+N FR KYPM+ +LQGTD +G FSGE+GGMIKAYNN + A L+Y Sbjct: 398 TKGASAFVEANYFRDCKYPMLSSLQGTDIFYDSKGDFSGEDGGMIKAYNNKIEGATRLVY 457 Query: 467 ANSNAGTAPANTTSFDAYLASSRNESVPSSYKALVGGTAYNNFDTSADTGVSASDIDSVS 526 AN + FDAYLASSRNE VPS+YK LVGGT YNNFDTS + + DS Sbjct: 458 ANQDP-------IQFDAYLASSRNEVVPSTYKTLVGGTTYNNFDTS--SVMYKYQPDSPD 508 Query: 527 TVEQIVTAEAGRMNGGDFTWAFNDSVDDASYALNSALMSAIRSYTTQLVSVGGNL 581 V++ VT+ AGR++GGD + F D+ DD S +N LM+ +++Y++ LVS G + Sbjct: 509 DVKESVTSYAGRVDGGDLIFNFTDA-DDTSSVINPDLMAKVKNYSSTLVSDGSKV 562 >gb|AAG59609.1| pectate lyase A [Clostridium cellulovorans] Length = 914 Score = 124 bits (310), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 76/156 (48%), Positives = 103/156 (66%), Gaps = 15/156 (9%) Query: 428 MMIALQGTDALGEGT---FSGENGGMIKAYNNTVVDAASLIYANSNAGTAPANTTSFDAY 484 M+ +LQG + + G FS E GGMIKAYNNTV A L+Y++ +A T FDAY Sbjct: 1 MLSSLQG-NCIYNGAVRGFSDEVGGMIKAYNNTVEGAKRLVYSSEDA-------TQFDAY 52 Query: 485 LASSRNESVPSSYKALVGGTAYNNFDTSADTGVSASDIDSVSTVEQIVTAEAGRMNGGDF 544 LA++RNE+VPSSYK + GGT YNNFDTS+ + + D+V V+ +VT AGR+ GGDF Sbjct: 53 LATTRNEAVPSSYKTVSGGTTYNNFDTSSI--MYSYKPDAVEDVKNVVTTYAGRVEGGDF 110 Query: 545 TWAFNDSVDDASYALNSALMSAIRSYTTQLV-SVGG 579 W F + DD + +++ALM+ I+SY + LV S GG Sbjct: 111 IWKFT-TADDTNDTIDAALMAKIKSYQSPLVASTGG 145 >pir||JC6502 pectate lyase (EC 4.2.2.2) - Amycolata sp gb|AAC38059.1| pectate lyase [Pseudonocardia sp.] Length = 310 Score = 114 bits (284), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 78/231 (33%), Positives = 117/231 (50%), Gaps = 31/231 (13%) Query: 260 NMTIEGIGNDAYAYGWGMLLRYVGNVEVRNLGVMLFPDDGISMDTGNVNVWVHNNDIFYG 319 N TIEG+G+ + G G+ + NV VRNL + DD I++ + VW+ +N G Sbjct: 87 NKTIEGVGSSSVITGQGLNIANASNVVVRNLTFRNWGDDAINVQY-STRVWIDHNSFSNG 145 Query: 320 SAGSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSEP------AEFFVTFHH 373 DG+ D+K+ S Y+T+S+N + K L+G S+ + VT+HH Sbjct: 146 Y----------DGAVDVKRASDYVTVSWNKFSSHNKTMLLGHSDDNGSEDRGKLRVTYHH 195 Query: 374 NWFDHSDSRHPRIRVAS-VHVYNNFYDGVSKYGVGVTTGASAFVESNVFRHAKYPMM--- 429 NWFD + RHPR+R + VHVYNN+Y GV+ YGV T A VE N F + P Sbjct: 196 NWFDGTQQRHPRVRFGNPVHVYNNYYGGVTSYGVASTMEAGVLVEGNYFENTGDPFHRGE 255 Query: 430 -------IALQGTDALGEGTFSGENGGMIKAYNNT-VVDAASLIYANSNAG 472 + +G + GT G+ GG +K+ + +D+AS + + AG Sbjct: 256 GSSPGGALVARGNHFVNSGT--GDQGGSVKSLPYSYPLDSASTVKSVVTAG 304 >ref|ZP_00067834.1| COG3866: Pectate lyase [Microbulbifer degradans 2-40] Length = 769 Score = 106 bits (265), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 69/220 (31%), Positives = 116/220 (52%), Gaps = 22/220 (10%) Query: 223 TPLAIRIIGKVTAAD---LSGQLNSSGYLEVKGKNNYSQMNMTIEGIGNDAYAYGWGMLL 279 TPL I + G + + +SG +++G E++ K N+++ G GN A G+ L Sbjct: 66 TPLIIYVSGTINHGNTEKVSGNCDTTG-DEIQFK---KVKNLSLIGTGNGAVFDQIGIHL 121 Query: 280 RYVGNVEVRNLGVMLFPD---------DGISMDTGNVNVWVHNNDIFYGSAGSDADQAKG 330 R N+ ++NL + D I M++G NVWV + ++ S ++ Sbjct: 122 RETSNIILQNLHIKNVKKSGSPTSNGGDAIGMESGVYNVWVDHCEL----EASGGEKDGY 177 Query: 331 DGSTDLKKGSTYITISYNHYWDSGKASLVGLSEPAEF--FVTFHHNWFDHSDSRHPRIRV 388 D D+K + Y+T+SY +Y DSG+ L+G S+ + +VTFHHN++ + DSR P +R Sbjct: 178 DSLLDMKATTQYVTVSYTYYHDSGRGGLMGSSDSDDTNTYVTFHHNYYKNMDSRLPLLRH 237 Query: 389 ASVHVYNNFYDGVSKYGVGVTTGASAFVESNVFRHAKYPM 428 + H +NN+YDG++K G+ G E+N F +A P+ Sbjct: 238 GTAHAFNNYYDGITKSGMNPRIGGQIKAENNYFENAHNPI 277 >ref|ZP_00067832.1| COG3866: Pectate lyase [Microbulbifer degradans 2-40] Length = 1316 Score = 106 bits (264), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 71/229 (31%), Positives = 120/229 (52%), Gaps = 24/229 (10%) Query: 215 LRQKGYDTTPLAIRIIGKVT---AADLSGQLNSSG-YLEVKGKNNYSQMNMTIEGIGNDA 270 + + D TPL I + G + A SG +++ ++ KG N S + G G+ A Sbjct: 59 MCNRASDDTPLIIYVTGTINHGNTAKYSGSCDTTADEIQFKGVKNISLI-----GTGSGA 113 Query: 271 YAYGWGMLLRYVGNVEVRNLGVMLFPD---------DGISMDTGNVNVWVHNNDIFYGSA 321 G+ LR N+ ++NL + D I M++G NVWV + ++ ++ Sbjct: 114 VFDQIGIHLRDTSNIILQNLHIKNVKKSGSPTSNGGDAIGMESGVYNVWVDHCEL--EAS 171 Query: 322 GSDADQAKGDGSTDLKKGSTYITISYNHYWDSGKASLVGLSEPAEF--FVTFHHNWFDHS 379 G ++D D D+K + Y+T+SY +Y DSG+ L+G S+ + FVTFHHN++++ Sbjct: 172 GGESDGY--DSLLDMKATTQYVTVSYTYYHDSGRGGLMGSSDSDDTNTFVTFHHNYYENM 229 Query: 380 DSRHPRIRVASVHVYNNFYDGVSKYGVGVTTGASAFVESNVFRHAKYPM 428 DSR P +R + H +NN+Y+G++K G+ G E+N F +A P+ Sbjct: 230 DSRLPLLRHGTAHAFNNYYNGIAKSGMNPRIGGQIKAENNYFENAHNPI 278 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.129 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 994,520,777 Number of extensions: 41668017 Number of successful extensions: 90723 Number of sequences better than 1.0e-05: 108 Number of HSP's gapped: 90640 Number of HSP's successfully gapped: 109 Length of query: 749 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 616 Effective length of database: 396,129,411 Effective search space: 244015717176 Effective search space used: 244015717176 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)