BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0074.0004.p (461 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA02119.1| TET.BSR [Bacillus subtilis] 422 e-117 prf||1703286A tetracycline resistance gene 422 e-116 ref|NP_391957.1| Na+/H+ and multifunctional antiporter [Bacillus... 422 e-116 ref|NP_040422.1| tetracycline resistance protein [Plasmid pLS1] ... 418 e-115 gb|AAL92527.1| tetracycline resistance protein [Enterococcus fae... 417 e-115 >dbj|BAA02119.1| TET.BSR [Bacillus subtilis] Length = 458 Score = 422 bits (1086), Expect = e-117, Method: Compositional matrix adjust. Identities = 199/443 (44%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA +F+ P++ANWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQG GA+A PAL+MV VARYI Sbjct: 73 SDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG++A YIHWSYL ++P T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 +G D+ G L+S GIV FMLFT+ Y++ +L IS+L FF F+Q IR+A+ PF++P L Sbjct: 193 ERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHIRKAQDPFVDPEL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G +L FL + F+D PW+++ ++ FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTV 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ +L L+P D + +LYSN+ Sbjct: 373 VSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +++F +++ ++ Y R++ Sbjct: 433 LILFAGTIVICWLVILNVYKRSR 455 >prf||1703286A tetracycline resistance gene Length = 458 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/443 (44%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA +F+ P++ANWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQG GA+A PAL+MV VARYI Sbjct: 73 SDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG++A YIHWSYL ++P T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 +G D+ G L+S GIV FMLFT+ Y++ +L IS+L FF F+Q IR+A+ PF++P L Sbjct: 193 ERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHIRKAQDPFVDPEL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G +L FL + F+D PW+++ ++ FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTV 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ +L L+P D + +LYSN+ Sbjct: 373 VSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +++F +++ ++ Y R++ Sbjct: 433 LILFAGIIVICWLVILNVYKRSR 455 >ref|NP_391957.1| Na+/H+ and multifunctional antiporter [Bacillus subtilis] sp|P23054|TCRB_BACSU Tetracycline resistance protein pir||YTBSY8 tetracycline resistance protein tetB - Bacillus subtilis dbj|BAA05208.1| tetracycline resistance protein [Bacillus subtilis] emb|CAA30827.1| TET protein [Bacillus subtilis] emb|CAB16114.1| multifunctional tetracycline-metal/H+ antiporter and Na+(K+)/H+ antiporter [Bacillus subtilis subsp. subtilis str. 168] Length = 458 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/443 (44%), Positives = 302/443 (68%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL +L+FFSV NE V NVSLPDIA +F+ P++ANWVNT+FM++F++G+ALYGK+ Sbjct: 13 NQVLIWLCVLSFFSVLNEMVLNVSLPDIANEFNKLPASANWVNTAFMLTFSIGTALYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQG GA+A PAL+MV VARYI Sbjct: 73 SDQLGIKNLLLFGIMVNGLGSIIGFVGHSFFPILILARFIQGIGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG++A YIHWSYL ++P T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSLVAMGEGVGPAIGGMVAHYIHWSYLLLIPTATIITVPFLIKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 +G D+ G L+S GIV FMLFT+ Y++ +L IS+L FF F+Q IR+A+ PF++P L Sbjct: 193 ERIRGHIDMAGIILMSAGIVFFMLFTTSYRFSFLIISILAFFIFVQHIRKAQDPFVDPEL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNVFFVIGTLCGGLIFGTVAGFVSMVPYMMKDVHHLSTAAIGSGIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 G+LVD++G + + +G +L FL + F+D PW+++ ++ FGGLSF KTV+ST Sbjct: 313 GGLLVDRKGSLYVLTIGSALLSSGFLIAAFFIDAAPWIMTIIVIFVFGGLSFTKTVISTV 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ +L L+P D + +LYSN+ Sbjct: 373 VSSSLKEKEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIGFLDHRLLPIDVDHSTYLYSNM 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +++F +++ ++ Y R++ Sbjct: 433 LILFAGIIVICWLVILNVYKRSR 455 >ref|NP_040422.1| tetracycline resistance protein [Plasmid pLS1] ref|NP_043524.1| tetracycline resistance protein [Plasmid pBC16] ref|NP_863350.1| TetL transporter [Enterococcus faecalis] sp|P11063|TCR_STRPN Tetracycline resistance protein pir||YTBSU6 tetracycline resistance protein - Bacillus cereus plasmid pBC16 pir||YTSOG tetracycline resistance protein - Streptococcus agalactiae plasmid pMV158 pir||JQ1211 tetracycline resistance protein - Bacillus sp. plasmid pTB19 gb|AAA98304.1| tetracycline resistance protein [Geobacillus stearothermophilus] dbj|BAA02943.1| ORF of tetracycline resistance gene (tetalpha1) [unidentified cloning vector] dbj|BAA20952.1| tetracycline resistance protein [synthetic construct] gb|AAA98167.1| tetracycline resistance protein emb|CAA35751.1| unnamed protein product [Bacillus cereus] dbj|BAA00005.1| tet protein [Bacillus subtilis] gb|AAB09024.1| tetracycline resistant protein [Enterococcus faecalis] gb|AAA84918.1| tetracycline resistance protein dbj|BAA21837.1| tetracycline resistance gene [Enterococcus faecalis] emb|CAB91631.1| tetracycline efflux protein [Cloning vector pCCR9] emb|CAB92188.1| tetracycline efflux protein [Cloning vector pAW9] gb|AAN03826.1| TetL transporter [Enterococcus faecalis] gb|AAN05731.1| tet resistance protein [Bacillus cereus] dbj|BAA14386.2| Tc resistance protein [synthetic construct] dbj|BAD00173.1| tetracycline resistance protein [Cloning vector pGETS109] Length = 458 Score = 418 bits (1074), Expect = e-115, Method: Compositional matrix adjust. Identities = 206/443 (46%), Positives = 298/443 (67%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL IL+FFSV NE V NVSLPDIA F+ P++ NWVNT+FM++F++G+A+YGK+ Sbjct: 13 NQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQGAGA+A PAL+MV VARYI Sbjct: 73 SDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG+IA YIHWSYL ++PM+T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 KG FD+ G L+SVGIV FMLFT+ Y +L +SVL F F++ IR+ PF++P L Sbjct: 193 VRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 GILVD+RG + + +G+ L VSFL+ S ++ T W ++ +V GGLSF KTV+ST Sbjct: 313 GGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTI 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ L L+P D + +LYSNL Sbjct: 373 VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNL 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +L+F +++ ++ Y +Q Sbjct: 433 LLLFSGIIVISWLVTLNVYKHSQ 455 >gb|AAL92527.1| tetracycline resistance protein [Enterococcus faecium] Length = 458 Score = 417 bits (1073), Expect = e-115, Method: Compositional matrix adjust. Identities = 206/443 (46%), Positives = 298/443 (67%) Query: 10 SKSMVWLSILAFFSVFNETVFNVSLPDIARQFDIRPSAANWVNTSFMISFAVGSALYGKI 69 ++ ++WL IL+FFSV NE V NVSLPDIA F+ P++ NWVNT+FM++F++G+A+YGK+ Sbjct: 13 NQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKL 72 Query: 70 SDXXXXXXXXXXXXXXXXXXXXIGIVFHKWFLVVLIARFIQGAGASAVPALIMVFVARYI 129 SD IG V H +F ++++ARFIQGAGA+A PAL+MV VARYI Sbjct: 73 SDQLGIKRLLLFGIIINCFGSLIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYI 132 Query: 130 EPNNKGKAFGMIGSMVAFSEGIGPAVGGVIADYIHWSYLFILPMMTLLTLPFFLHVLPDE 189 N+GKAFG+IGS+VA EG+GPA+GG+IA YIHWSYL ++PM+T++T+PF + +L E Sbjct: 133 PKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKE 192 Query: 190 PSKKGKFDVLGASLLSVGIVMFMLFTSMYQWGYLAISVLCFFGFLQRIRRAKQPFIEPSL 249 KG FD+ G L+SVGIV FMLFT+ Y +L +SVL F F++ IR+ PF++P L Sbjct: 193 VRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGL 252 Query: 250 LRRKKFXXXXXXXXXXXXXXXXYISTVPYMMKDVYLMPTSLIGSAVLFPGTISVLLFGFV 309 + F ++S VPYMMKDV+ + T+ IGS ++FPGT+SV++FG++ Sbjct: 253 GKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYI 312 Query: 310 SGILVDKRGCFFTMLVGLFMLVVSFLSISLFVDRTPWLISTTMVLTFGGLSFIKTVVSTS 369 GILVD+RG + + +G+ L VSFL+ S ++ T W ++ +V GGLSF KTV+ST Sbjct: 313 GGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTI 372 Query: 370 VAEALSSDESGSGMSFLNFACFLAEGIGVSYAGGLLTMSWLKFPLIPTVTDTAAFLYSNL 429 V+ +L E+G+GMS LNF FL+EG G++ GGLL++ L L+P D + +LYSNL Sbjct: 373 VSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNL 432 Query: 430 MLVFITAVILGGIIFSLAYGRNQ 452 +L+F +++ ++ Y +Q Sbjct: 433 LLLFSGIIVISWLVTLNVYKHSQ 455 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.142 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 583,623,200 Number of extensions: 22317643 Number of successful extensions: 88506 Number of sequences better than 1.0e-05: 689 Number of HSP's gapped: 88272 Number of HSP's successfully gapped: 689 Length of query: 461 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 332 Effective length of database: 406,989,807 Effective search space: 135120615924 Effective search space used: 135120615924 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)