BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0073.0014.p (516 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242776.1| two-component response regulator [Bacillus halo... 283 5e-75 ref|NP_242975.1| BH2109~unknown conserved protein [Bacillus halo... 164 3e-39 ref|NP_607656.1| putative two-component sensor response regulato... 136 2e-30 ref|ZP_00059682.1| COG4753: Response regulator containing CheY-l... 122 2e-26 ref|NP_563003.1| two-component response regulator [Clostridium p... 117 5e-25 >ref|NP_242776.1| two-component response regulator [Bacillus halodurans] pir||F83888 two-component response regulator BH1910 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05629.1| two-component response regulator [Bacillus halodurans] Length = 506 Score = 283 bits (725), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 175/514 (34%), Positives = 282/514 (54%), Gaps = 19/514 (3%) Query: 1 VLIVDDELAIREGLATLLDWESLGYVVVDTAANAIEAKHKYELHSPDLMIIDIRMPGRDG 60 V+IVDDE +REGL L+ WE G+ V+DTA N E KY SPDLMI+DIRMP DG Sbjct: 4 VMIVDDEAMVREGLKALISWEEYGFRVIDTARNGKEGFTKYRQLSPDLMIVDIRMPEVDG 63 Query: 61 LELIKELREDDPELHIIILSGYADFSYAKRAMSLGIDNYLLKPVDEDELHLYLTNLSAEL 120 L LI+ +R +D +H IILSG++DF+YAK+A+ D+YLLKP+DEDEL L + + Sbjct: 64 LSLIESIRAEDSAIHFIILSGHSDFNYAKKALGCQCDDYLLKPLDEDELIDSLKKVYGRI 123 Query: 121 AARAVYRKKDAAVKIWSREMLVQSLLLDNGPQAPAALEGPAMESGLLWDSYQVVLIRLLL 180 + V+ EM++ LL + P + + E YQV L+ Sbjct: 124 TRHHQVHELVERVQEKQVEMVLTELL-EGTPLSEIPI---GKEHNFFATDYQVALV---- 175 Query: 181 QDNADNGPSTAVKTRLAASFEEQEWGIVFSLDSYLGVLLQPSYQKELVRQLMVRNIWEAV 240 +N K +L EEQE G V Y+ ++ + + ++ + + + Sbjct: 176 ----ENRQDRLTK-QLKRLIEEQEEGYVIPFPEYIVIVCKNRFLQKRGKDHRYHLFAKWL 230 Query: 241 TAQGLECIITAGDMVDSLEEVPASKESALARMKEHFFYDEPGMIGPDSLKLKTGLPVKPE 300 + L AG V +L ++ S +SA ++ FF ++ + D L ++ KPE Sbjct: 231 EEEELHA--CAGSKVVNLADIYESYQSAKRLYEKKFFVEKGRLFTADQLVDRS----KPE 284 Query: 301 EVEPGLAPVTERLYFAMDIGSLNLLPSLIQEAGALMIAAGYSEMAVKSRYVRLVTHLMNK 360 E L +RLY+++DIG++ ++I+E ++I E +K +L ++++++ Sbjct: 285 APEFSLESYADRLYYSIDIGNMEGCVAIIEELKNVVIVHLQEEKEIKVALFQLYSNILHQ 344 Query: 361 LSLQYRELELLHSRMDEQVEQIYKHTSLPKLQSHINVLLEQYAGGIKRDDMEVLIKRMLD 420 L + +E + + IY+ LP + + + L+ + ++L+++M+ Sbjct: 345 LVRSHPNMEGVLEPLVMNAGDIYQFEHLPGIMAFLTKQLKYVIDCMDNGQPDLLLRKMIA 404 Query: 421 LIHRHYPENLKLETLADVFTYSSAYLGKLFKSSTGLSFNSYLDNIRIGKAKELLDQGYKI 480 LIH+ Y +NL+LETLA VF Y+ AYLGKLFK+ TG FN+YLD +RI K LL +GYK+ Sbjct: 405 LIHKDYAKNLRLETLASVFNYNRAYLGKLFKNYTGEYFNTYLDKVRIENGKRLLQKGYKV 464 Query: 481 HQAASEVGFSDVDYFREKFKKIEGLSPSDYRRKN 514 ++ A ++G+++VDYF KFKK G+SPS +R+++ Sbjct: 465 YEVAEKIGYANVDYFHRKFKKYVGVSPSTFRKRH 498 >ref|NP_242975.1| BH2109~unknown conserved protein [Bacillus halodurans] pir||E83913 hypothetical protein BH2109 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05828.1| BH2109~unknown conserved protein [Bacillus halodurans] Length = 525 Score = 164 bits (416), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 141/560 (25%), Positives = 261/560 (46%), Gaps = 88/560 (15%) Query: 1 VLIVDDELAIREGLATLLDWESLGYVVVDTAANAIEAKHKYELHSPDLMIIDIRMPGRDG 60 VL+ DDE +R GL +L++WE GY V A N +A + PDL+I DIRMP DG Sbjct: 4 VLLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPVFDG 63 Query: 61 LELIKELRED-DPELHIIILSGYADFSYAKRAMSLGIDNYLLKPVDEDELHLYLTNLSAE 119 L+LI+++ E II+SGY DFSYA++A+ G +++LKP+D+DEL L L+A Sbjct: 64 LQLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKLAAT 123 Query: 120 LAARAVY-------------------RKKDAAVKIWSREMLVQS------LLLDNGPQAP 154 L KK+ V+ W+ ++ + + LLL+ Sbjct: 124 LDQEKATNSDQEKVLLDQIFIHSLGENKKENEVRRWAAKLNINNRHFYYYLLLE------ 177 Query: 155 AALEGPAMESGLLWDSYQVVLIRLLLQDNADNGPSTAVKTRLAASFEEQEWGIVFSLDSY 214 + +++ G + + Y+ + +++ + A R AA+ E+ I LD Sbjct: 178 --VNNLSLDKGFINEQYKEPIRKVIRETVA----------RQAAT--EEYTAIYEQLDHA 223 Query: 215 LGVLLQPSYQKELVRQL-----MVRNIWEAVTAQGLECIITAGDMVDSLEEVPASKESAL 269 G+L+ + K+ Q+ +R + T + + G D L + S E+A Sbjct: 224 YGLLITSEHLKKFNGQIEPLAEKLRKDIQDFT--NIPLTVYVGAKTDDLSTIKKSYETAN 281 Query: 270 ARMKEHFFYDEPGMIGPDSLKLKTGLPVKPEEVEPGLAPVTERLYFA----MDIGSLNLL 325 + + ++E + + + E V+ + L+ M+ + + + Sbjct: 282 SLRNYKYIFNEKNVF----------IHEQTESVDTTYDELNNHLFVTLMEQMEENNSDRI 331 Query: 326 PSLIQEAGALMIAAGYSEMAVKSRYVRLVTHLMNK-----------LSLQYRELELLHSR 374 ++I + + + AVK+ R V +M +S Q ++ Sbjct: 332 VNIIDKIFSEFQTKKFVPSAVKTSVNRCVHKVMQTVQAMEGDTGKIISAQAMTNWESYNL 391 Query: 375 MDEQVEQIYKHTSLPKLQSHINVLLEQYAGGIKRDDMEVLIKRMLDLIHRHYPENLKLET 434 +Q++Q++ K V++++ +++++M+ I ++ I HY EN+ L++ Sbjct: 392 TFDQLKQVF-----TKFVLEAAVIIQE----LRKENMKSDIYKVKTYIENHYHENISLKS 442 Query: 435 LADVFTYSSAYLGKLFKSSTGLSFNSYLDNIRIGKAKELLDQG-YKIHQAASEVGFSDVD 493 +A +F + Y+G+LFK + G+ F YL +R+ +AK+LL Q ++++ A VGF D Sbjct: 443 IASLFYMNPVYMGQLFKKTFGVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTD 502 Query: 494 YFREKFKKIEGLSPSDYRRK 513 YF +F+KI ++P++YR K Sbjct: 503 YFVTQFEKINKMTPTEYRNK 522 >ref|NP_607656.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS8232] gb|AAL98155.1| putative two-component sensor response regulator [Streptococcus pyogenes MGAS8232] Length = 494 Score = 136 bits (342), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 146/547 (26%), Positives = 241/547 (44%), Gaps = 102/547 (18%) Query: 1 VLIVDDELAIREGLATLLDWESLGYVVVDTAANAIEAKHKYELHSPDLMIIDIRMPGRDG 60 VL+VDDE I +GL ++DW++LG+ VV TA + EA + D+MI D+ MPG G Sbjct: 4 VLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALTYLTQYPVDVMISDVTMPGMTG 63 Query: 61 LELIKELREDDPELHIIILSGYADFSYAKRAMSLGIDNYLLKPVDEDELHLYLTNLSAEL 120 L+LI+ + P+L +ILSGY +FSY ++AM L YLLKPVD+ EL + L Sbjct: 64 LDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPVDKAELQAKMKQFKDCL 123 Query: 121 AARAVYRKK-----DAAVKIWSREMLVQSLLLDNGPQAP-AALEGPAMESGLLWDSYQVV 174 A+ + D+ + +W + L + P AAL G Sbjct: 124 DAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQGLPAAALTG-------------FT 170 Query: 175 LIRLLLQDNADNGPSTAVKTRLAASFEEQEWGIVFSLDS------YLGVLLQPSYQKEL- 227 ++ L Q+ + + A FE + G F L YL VLL + Q + Sbjct: 171 VLYLDCQE---------WQAAIDAYFERE--GQPFYLKKEEDKKVYLAVLLGKTDQAKAF 219 Query: 228 ---VRQLMVRNIWEAVTAQGLECIITAGDMVDSLEEVPASKESALARMKEHFFYDEPGMI 284 +++ RN+ + + G+ VD E V S L + FY+ + Sbjct: 220 TYDLQKRFSRNMHQLI----------LGETVDDWENVYESYNQVLKSL----FYN--CEV 263 Query: 285 GPDSLKLKTGLPVKPEEV--------------EPGLAPVTERLYFAMDIGSLNLLPSLIQ 330 P K K+ + + PE EP + E ++ M +LN P ++ Sbjct: 264 SPIRAKDKSKIEL-PESRLQFFAFNKALMIGDEPTILSKLEAIFDEM--KTLNFSPEDVK 320 Query: 331 EAGALMIAAGYSEMAV--KSRYVRLVTHLMNKLSLQYRELELLHSRMDEQVEQIYKHTSL 388 L+ + Y + + K Y+ +V + + S+ R L L +D + + Sbjct: 321 HVSFLLFSDIYRQFPILDKMTYLSMVKTIHDSQSID-RILRELKKVLD-----VTNQNNS 374 Query: 389 PKLQSHINVLLEQYAGGIKRD-DMEVLIKRMLDLIHRHYPENLKLETLADVFTYSSAYLG 447 P + + L+ + I+++ E+ +K + D +H + YLG Sbjct: 375 P--EKRYSDLVSETIDCIRKEYHQELTLKAIADRLHVN-----------------GVYLG 415 Query: 448 KLFKSSTGLSFNSYLDNIRIGKAKE-LLDQGYKIHQAASEVGFSDVDYFREKFKKIEGLS 506 + FK+ T SF YL+++RI KA++ LL I++ A E G++ YF + FKK+ GLS Sbjct: 416 QCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSINEIAYETGYNTNHYFIKMFKKLNGLS 475 Query: 507 PSDYRRK 513 P ++R + Sbjct: 476 PKEFRDR 482 >ref|ZP_00059682.1| COG4753: Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain [Clostridium thermocellum ATCC 27405] Length = 540 Score = 122 bits (307), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 131/555 (23%), Positives = 244/555 (43%), Gaps = 64/555 (11%) Query: 2 LIVDDELAIREGLATLLDWESLGYVVVDTAANAIEAKHKYELHSP-DLMIIDIRMPGRDG 60 ++VDD+ IRE ++W +G+ + +A + I+A +K H+ DL+I DI +P +G Sbjct: 1 MVVDDDPKIREWFEVEIEWGKMGFDFICSAKDGIDALNKLNQHNKLDLVISDIDIPKMNG 60 Query: 61 LELIKELREDDPELHIIILSGYADFSYAKRAMSLGIDNYLLKPVDEDELHLYLTNLSAEL 120 +EL+ ++E + I++LS + ++ K+ + LG +Y+LKP+D+D++ L L Sbjct: 61 IELLNSIKEYNLSSMIVLLSDENNMAHVKQGLLLGAFDYILKPLDKDKVIDVLKKAHDSL 120 Query: 121 AARAVYRKKDAAVK-------IWSREMLVQSLLLDNGPQAPAALEGPAMESGLLWDSYQV 173 + + +++ +K SR+ ++Q LL E P E + + Y + Sbjct: 121 MDKKMEEERNKNLKKKLELNLSLSRDKILQDLL--------RGKEFPFQELDYIQNEYNI 172 Query: 174 VLIRLLLQ------DNADNGPSTAVKT-RLAASFEEQEWGIVFSLDSY------------ 214 L + ++Q N D +K+ R + EE I +L + Sbjct: 173 SLQKGMVQVGIIEIGNFDADSRELIKSGRFDSLVEEVGKIISNTLSEFPELNCNMVEMDI 232 Query: 215 --LGVLLQPSYQKELVR-QLMVRNIWEAVTA-----QGLECIITAGDMVDSLEEVPASKE 266 + V+LQP QKEL + M + +E V + I G SL ++ S Sbjct: 233 GLISVILQPVGQKELQDFEDMTADFFEKVLKGIKQDANMRATIGIGGAYSSLRDISQSYM 292 Query: 267 SALARMKEHFFYDEPGMIGPDSLKLKTGLPVK-----PEEVEPGLAPVTERLYFAMDIGS 321 A A ++ F + G + +KT K P E E L + D + Sbjct: 293 GAKAALRHKFI-----LGGNRVIHIKTDYNEKQNLLYPAEREKMLVESI----MSGDDNA 343 Query: 322 LNLLPSLIQEAGALMIAAGYSEMAVKSRYVRLVTHLMNKLSLQYRELELLHSRMDEQVEQ 381 L L ++ + + I G + + +LV ++ N + +QY + L+ Sbjct: 344 LKLAENMFDD---IAIGTGDNLKRIAFAANQLVFNISNFIDMQYDFINKLYDFRKFNNMD 400 Query: 382 IYKHTSLPKLQSHINVLLEQYAGGIKR---DDMEVLIKRMLDLIHRHYPENLKLETLADV 438 K +S +++ + + +K LIK+ + + H + + L T+AD Sbjct: 401 FSKFSSKDEIKEFFLSFVTELLNVVKEYKPAQNNTLIKKACEYVLNHIDQEITLMTIADY 460 Query: 439 FTYSSAYLGKLFKSSTGLSFNSYLDNIRIGKAKELLDQG-YKIHQAASEVGFSDVDYFRE 497 S Y LFK TG +F Y+ +++ AK+LL +G YK ++ + +G+ + YF Sbjct: 461 LNISKNYFCSLFKQETGYNFLEYVTKVKMEWAKKLLREGNYKTYEVSEMLGYREASYFSR 520 Query: 498 KFKKIEGLSPSDYRR 512 F+K LSP++Y++ Sbjct: 521 LFRKYTKLSPAEYKK 535 >ref|NP_563003.1| two-component response regulator [Clostridium perfringens] dbj|BAB81793.1| two-component response regulator [Clostridium perfringens str. 13] Length = 507 Score = 117 bits (294), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 55/115 (47%), Positives = 83/115 (72%) Query: 1 VLIVDDELAIREGLATLLDWESLGYVVVDTAANAIEAKHKYELHSPDLMIIDIRMPGRDG 60 V++ DDE I +GL +++WE LG +V+ A+N EA K++ + D+++ DI MP G Sbjct: 4 VMLADDENLILQGLENIIEWEELGLEIVNKASNGQEAIDKFKENPVDIVVTDINMPQVTG 63 Query: 61 LELIKELREDDPELHIIILSGYADFSYAKRAMSLGIDNYLLKPVDEDELHLYLTN 115 LEL+KEL++ + ++ IILSGY DFSYAK+A+ LG++NY+LKP+DE+EL L N Sbjct: 64 LELLKELKKINSDVKFIILSGYDDFSYAKKAIELGVENYILKPIDEEELEKTLKN 118 Score = 76.3 bits (186), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 38/100 (38%), Positives = 62/100 (62%), Gaps = 1/100 (1%) Query: 414 LIKRMLDLIHRHYPENLKLETLADVFTYSSAYLGKLFKSSTGLSFNSYLDNIRIGKAKEL 473 +I++++ ++ +Y E + L+TLA + +++YLG++F G SF+ YL+ + KAKEL Sbjct: 404 VIQQIISYVNENYYEEVSLKTLAQKYHINTSYLGQVFTKEVGCSFSEYLNKTKNMKAKEL 463 Query: 474 -LDQGYKIHQAASEVGFSDVDYFREKFKKIEGLSPSDYRR 512 L+ KI+ A +VG+ D YF KFKK G+ PS R Sbjct: 464 ILNTNMKINDIAKKVGYLDTSYFYRKFKKYYGVCPSTLRN 503 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 745,163,430 Number of extensions: 30546168 Number of successful extensions: 100733 Number of sequences better than 1.0e-05: 3008 Number of HSP's gapped: 102515 Number of HSP's successfully gapped: 3066 Length of query: 516 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 386 Effective length of database: 404,274,708 Effective search space: 156050037288 Effective search space used: 156050037288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)