BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0005.p (465 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_842580.1| D-alanyl-D-alanine carboxypeptidase [Bacillus a... 294 3e-78 ref|NP_653964.1| Peptidase_S11, D-alanyl-D-alanine carboxypeptid... 294 3e-78 ref|NP_976338.1| D-alanyl-D-alanine carboxypeptidase [Bacillus c... 293 9e-78 ref|NP_829918.1| D-alanyl-D-alanine carboxypeptidase [Bacillus c... 291 2e-77 ref|NP_978837.1| D-alanyl-D-alanine carboxypeptidase [Bacillus c... 290 7e-77 >ref|NP_842580.1| D-alanyl-D-alanine carboxypeptidase [Bacillus anthracis str. Ames] gb|AAP24066.1| D-alanyl-D-alanine carboxypeptidase [Bacillus anthracis str. Ames] Length = 435 Score = 294 bits (753), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 169/402 (42%), Positives = 236/402 (58%), Gaps = 21/402 (5%) Query: 68 SLGLKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVV 127 +L ++ +A+L+E +G++L NAD L ASMTKMM+EYLV EAV+KG+ WDQK+ V Sbjct: 33 ALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHEAVDKGKFKWDQKIKV 92 Query: 128 QENASKQIG----SRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMM 183 E A K S + L G +TVKELY AMA+ SAN AT+ALAE +AG E +FV MM Sbjct: 93 SEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMM 152 Query: 184 NETAQKMGMKTAYFINSTGLNRADMPAKFQPD---TDRETVMSAMDAAILAKHIVTDHPD 240 N+ ++++G+K F+NSTGL D+ P+ D E MSA D A LA+H++ D+P Sbjct: 153 NDKSKELGLKNYKFVNSTGLTNKDLKG-MHPEGTTADEENKMSAKDVATLAQHLIKDYPK 211 Query: 241 FTDFTTIQSYKFR-DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCF 299 D I +FR +++K M N+NWML+ Y G+DGLKTG T +AG+CF Sbjct: 212 VLDTAKIPKKEFRPEKEKFAMSNWNWMLKG-------LVKEYDGVDGLKTGSTPEAGDCF 264 Query: 300 TGTAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVL 359 TGT R+GMR ISVV+ S RF ETKK+ D+GF NFE+KQ+ + + G ETV V Sbjct: 265 TGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQETVRVE 324 Query: 360 KGKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVE 419 K+K V V T ++ VPKG S ++ + API + +G++ + + Sbjct: 325 NAKDKDVAVQTKQAISLPVPKG--SKEVYKTELKESSKGQEAPIKKGAALGQMVITPKDT 382 Query: 420 ---GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 G + V+L+T E E+A WF MR IG FF ++ Sbjct: 383 NDPGFLTGKSLQVDLVTTSEVEEANWFTRAMRGIGSFFSGIW 424 >ref|NP_653964.1| Peptidase_S11, D-alanyl-D-alanine carboxypeptidase [Bacillus anthracis A2012] Length = 438 Score = 294 bits (753), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 169/401 (42%), Positives = 235/401 (58%), Gaps = 19/401 (4%) Query: 68 SLGLKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVV 127 +L ++ +A+L+E +G++L NAD L ASMTKMM+EYLV EAV+KG+ WDQK+ V Sbjct: 36 ALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHEAVDKGKFKWDQKIKV 95 Query: 128 QENASK----QIGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMM 183 E A K S + L G +TVKELY AMA+ SAN AT+ALAE +AG E +FV MM Sbjct: 96 SEYAYKVSQDAXLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMM 155 Query: 184 NETAQKMGMKTAYFINSTGLNRADMPAKFQPDT--DRETVMSAMDAAILAKHIVTDHPDF 241 N+ ++++G+K F+NSTGL D+ T D E MSA D A LA+H++ D+P Sbjct: 156 NDKSKELGLKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKV 215 Query: 242 TDFTTIQSYKFR-DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFT 300 D I +FR +++K M N+NWML+ Y G+DGLKTG T +AG+CFT Sbjct: 216 LDTAKIPKKEFRPEKEKFAMSNWNWMLKG-------LVKEYDGVDGLKTGSTPEAGDCFT 268 Query: 301 GTAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLK 360 GT R+GMR ISVV+ S RF ETKK+ D+GF NFE+KQ+ + + G ETV V Sbjct: 269 GTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQETVRVEN 328 Query: 361 GKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVE- 419 K+K V V T ++ VPKG S ++ + API + +G++ + + Sbjct: 329 AKDKDVAVQTKQAISLPVPKG--SKEVYKTELKESSKGQEAPIKKGAALGQMVITPKDTN 386 Query: 420 --GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 G + V+L+T E E+A WF MR IG FF ++ Sbjct: 387 DPGFLTGKSLQVDLVTTSEVEEANWFTRAMRGIGSFFSGIW 427 >ref|NP_976338.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 10987] gb|AAS38946.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 10987] Length = 438 Score = 293 bits (749), Expect = 9e-78, Method: Compositional matrix adjust. Identities = 169/401 (42%), Positives = 235/401 (58%), Gaps = 19/401 (4%) Query: 68 SLGLKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVV 127 +L ++ +A+L+E +G+++ NAD L ASMTKMM+EYLV EAV+KG+L WDQK+ V Sbjct: 36 ALNIEAGAAILVEANSGKIVYQKNADELLSIASMTKMMSEYLVHEAVDKGKLKWDQKIKV 95 Query: 128 QENASKQIG----SRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMM 183 E A K S + L G +TVKELY AMA+ SAN AT+ALAE +AG E +FV MM Sbjct: 96 SEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMM 155 Query: 184 NETAQKMGMKTAYFINSTGLNRADMPAKFQPDT--DRETVMSAMDAAILAKHIVTDHPDF 241 N+ ++++G+K F+NSTGL D+ T D E MSA D A LA+H++ D+P Sbjct: 156 NDKSKELGLKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKV 215 Query: 242 TDFTTIQSYKFR-DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFT 300 D I +FR +++K M N+NWML+ Y G+DGLKTG T +AG+CFT Sbjct: 216 LDTAKIPKKEFRPEKEKFAMSNWNWMLKG-------LVKEYDGVDGLKTGSTPEAGDCFT 268 Query: 301 GTAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLK 360 GT R+GMR ISVV+ S RF ETKK+ D+GF NFE+KQ+ + + G ETV V Sbjct: 269 GTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKGQETVRVEN 328 Query: 361 GKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVE- 419 K+K V V T ++ VPKG S + + API + +G++ + + Sbjct: 329 AKDKDVAVQTKQAISLPVPKG--SKDVYKTELKESSKGQEAPIKKGAALGQMVITPKDTN 386 Query: 420 --GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 G + V+L+T E E+A WF MR IG FF ++ Sbjct: 387 DPGFLTGKSLQVDLVTTAEVEEANWFTRAMRGIGSFFSGIW 427 >ref|NP_829918.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 14579] gb|AAP07119.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 14579] Length = 438 Score = 291 bits (746), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 170/401 (42%), Positives = 234/401 (58%), Gaps = 19/401 (4%) Query: 68 SLGLKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQKVVV 127 +L ++ +A+L+E +G++L NAD L ASMTKMM+EYLV EAV+KG+L WDQK+ V Sbjct: 36 ALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHEAVDKGKLKWDQKIKV 95 Query: 128 QENASKQIG----SRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFVAMM 183 E A K S + L G +TVKELY AMA+ SAN AT+ALAE +AG E +FV MM Sbjct: 96 SEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMM 155 Query: 184 NETAQKMGMKTAYFINSTGLNRADMPAKFQPDT--DRETVMSAMDAAILAKHIVTDHPDF 241 N+ ++++G+K +NSTGL D+ T D E MSA D A LA+H++ D+P Sbjct: 156 NDKSKELGVKNYKCVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKV 215 Query: 242 TDFTTIQSYKFR-DRDKAPMINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAGNCFT 300 D I +FR +++K M N+NWML+ Y G+DGLKTG T +AG+CFT Sbjct: 216 LDTAKIPKKEFRPEKEKFGMSNWNWMLKG-------LVKEYDGVDGLKTGSTPEAGDCFT 268 Query: 301 GTAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETVPVLK 360 GT R+GMR ISVV+ S RF ETKK+ D+GF NFEVKQ+ + + G ETV V Sbjct: 269 GTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEVKQMYKKGSSVKGQETVRVEN 328 Query: 361 GKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSYQVE- 419 K+K V V T ++ VPKG S + + API + +G++ + + Sbjct: 329 AKDKDVAVQTKQAISLPVPKG--SKDVYKTELKESSKGQEAPIKKGAALGQMVITPKDTN 386 Query: 420 --GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 G + V+L+T E E+A WF MR IG FF ++ Sbjct: 387 DPGFLTGKSLQVDLVTTSEVEEANWFTRAMRGIGSFFSGMW 427 >ref|NP_978837.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 10987] gb|AAS41445.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 10987] Length = 434 Score = 290 bits (741), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 173/405 (42%), Positives = 232/405 (57%), Gaps = 20/405 (4%) Query: 65 SVESLGLKLRSAVLLEPTTGEVLLSLNADVPLPPASMTKMMTEYLVTEAVNKGQLSWDQK 124 +V L ++ SA+L+E +G++L NAD L ASMTKMM+EYLV EAV KG+L WDQK Sbjct: 30 TVPVLDVEAGSAILVEANSGKILYEKNADESLAIASMTKMMSEYLVHEAVEKGKLKWDQK 89 Query: 125 VVVQENA----SKQIGSRIFLAQGDEHTVKELYIAMAVGSANDATVALAEEVAGSEQEFV 180 V + E A + S + L +TVKELY AMA+ SAN AT+AL EE+AG E +FV Sbjct: 90 VKISEYAYTISQDRSLSNVPLENSGSYTVKELYEAMAIYSANGATIALVEEIAGKEADFV 149 Query: 181 AMMNETAQKMGMKTAYFINSTGLNRADMPAKFQPDT--DRETVMSAMDAAILAKHIVTDH 238 MMN+ +++ GMK F+NSTGL D+ T D + MSA D AILA+ +V D Sbjct: 150 KMMNDKSKEFGMKNYKFVNSTGLTNYDLKGHHPEGTTPDEKNKMSARDCAILAQRLVQDF 209 Query: 239 PDFTDFTTIQSYKFRDRDKAP--MINYNWMLEANKNVTNFKAYAYPGLDGLKTGHTNDAG 296 P D I F+ K P M+N+N ML+ Y G+DGLKTG T +AG Sbjct: 210 PKMLDTAKIPKKTFQKGGKYPIDMVNFNMMLKG-------LIKQYEGVDGLKTGTTPEAG 262 Query: 297 NCFTGTAVRDGMRLISVVMGADSEPHRFTETKKVLDFGFNNFEVKQVVAPKAVIAGNETV 356 +CFTGT R+GMRLISVV+ A+S RF ETKK+ D+GF NFEVK + +V+ G+ETV Sbjct: 263 DCFTGTVERNGMRLISVVIKANSHTARFDETKKLYDYGFANFEVKNIYGKGSVVKGHETV 322 Query: 357 PVLKGKNKTVPVVTDAGVTFIVPKGTASPQIKTAVVINDPATLVAPIAGASKVGKVTYSY 416 V K+K V V T ++ +PKG K V N+ API + K+ S Sbjct: 323 RVANAKDKDVIVQTKQAISLPMPKGNKDVYKKEFKVSNEEQE--APIKKGVTISKMVISP 380 Query: 417 QVE---GITQVQEKTVNLITAEEAEKAGWFKLLMRAIGEFFGDLF 458 + G + ++L+T + E+A WF MR IG FF ++ Sbjct: 381 KDNTDPGFLSGKSLQIDLVTKSDVEQANWFTRFMRKIGSFFNGMW 425 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 616,489,482 Number of extensions: 24261735 Number of successful extensions: 66787 Number of sequences better than 1.0e-05: 722 Number of HSP's gapped: 65681 Number of HSP's successfully gapped: 722 Length of query: 465 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 336 Effective length of database: 406,989,807 Effective search space: 136748575152 Effective search space used: 136748575152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)