BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0063.0002.p (194 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAC06853.1| 2-deoxy-scyllo-inosose synthase 20kDa subunit [B... 246 2e-64 ref|NP_240889.1| amidotransferase [Bacillus halodurans] >gi|2531... 221 8e-57 pdb|1Q7R|A Chain A, X-Ray Crystallographic Analysis Of A Predict... 198 6e-50 ref|NP_387893.1| similar to hypothetical proteins [Bacillus subt... 196 3e-49 ref|NP_829920.1| pyridoxine biosynthesis amidotransferase [Bacil... 193 2e-48 >dbj|BAC06853.1| 2-deoxy-scyllo-inosose synthase 20kDa subunit [Bacillus circulans] Length = 190 Score = 246 bits (628), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 123/189 (65%), Positives = 142/189 (75%) Query: 1 MKIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGF 60 MK+GVLALQGAV EHI IE G +G+ +KRA STTIGKLMR+YGF Sbjct: 1 MKVGVLALQGAVAEHIRLIEAVGGEGVVVKRAEQLAELDGLIIPGGESTTIGKLMRRYGF 60 Query: 61 IEAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLD 120 IEAIRDF+ +GK VFGTCAG+IV+A +I G E AHL LMD+TV RNAFGRQRESFE DL Sbjct: 61 IEAIRDFSNQGKAVFGTCAGLIVIADKIAGQEEAHLGLMDMTVQRNAFGRQRESFETDLP 120 Query: 121 VKGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLHQ 180 VKGID PVRAVFIRAPLI +VG VDVL+ Y +IV ARQG+LL +SFHPELTDD +H Sbjct: 121 VKGIDRPVRAVFIRAPLIDQVGNGVDVLSEYNGQIVAARQGHLLAASFHPELTDDSSMHA 180 Query: 181 YFADMVEHS 189 YF DM+ + Sbjct: 181 YFLDMIREA 189 >ref|NP_240889.1| amidotransferase [Bacillus halodurans] pir||G83652 amidotransferase BH0023 [imported] - Bacillus halodurans (strain C-125) dbj|BAB03742.1| amidotransferase [Bacillus halodurans] Length = 196 Score = 221 bits (562), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 112/194 (57%), Positives = 138/194 (71%) Query: 1 MKIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGF 60 +KIGVLALQGAV EH+ +E G + +K+ STT+ +L+ KYGF Sbjct: 2 VKIGVLALQGAVREHVRCLEAPGVEVSIVKKVEQLEDLDGLVFPGGESTTMRRLIDKYGF 61 Query: 61 IEAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLD 120 E ++ FA +GKPVFGTCAG+I++A RIDG + HLELMD+TV RNAFGRQRESFE DL Sbjct: 62 FEPLKAFAAQGKPVFGTCAGLILMATRIDGEDHGHLELMDMTVQRNAFGRQRESFETDLI 121 Query: 121 VKGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLHQ 180 V+G+ + VRAVFIRAPLI EVG +VDVL+ + DEIV ARQG+LL SFHPELTDD R HQ Sbjct: 122 VEGVGDDVRAVFIRAPLIQEVGQNVDVLSKFGDEIVVARQGHLLGCSFHPELTDDRRFHQ 181 Query: 181 YFADMVEHSAAANQ 194 YF MV+ + Q Sbjct: 182 YFVQMVKEAKTIAQ 195 >pdb|1Q7R|A Chain A, X-Ray Crystallographic Analysis Of A Predicted Amidotransferase From B. Stearothermophilus At 1.9 A Resolution Length = 219 Score = 198 bits (503), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 101/192 (52%), Positives = 126/192 (65%) Query: 2 KIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGFI 61 KIGVL LQGAV EH+ +IE GA+ + +K++ STT +L+ +YG Sbjct: 25 KIGVLGLQGAVREHVRAIEACGAEAVIVKKSEQLEGLDGLVLPGGESTTXRRLIDRYGLX 84 Query: 62 EAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLDV 121 E ++ FA GKP FGTCAG+I+LAKRI G + HL L DITV RN+FGRQRESFE +L + Sbjct: 85 EPLKQFAAAGKPXFGTCAGLILLAKRIVGYDEPHLGLXDITVERNSFGRQRESFEAELSI 144 Query: 122 KGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLHQY 181 KG+ + VFIRAP I E G VDVL Y D IV ARQG L SFHPELTDD+RL QY Sbjct: 145 KGVGDGFVGVFIRAPHIVEAGDGVDVLATYNDRIVAARQGQFLGCSFHPELTDDHRLXQY 204 Query: 182 FADMVEHSAAAN 193 F + V+ + A+ Sbjct: 205 FLNXVKEAKXAS 216 >ref|NP_387893.1| similar to hypothetical proteins [Bacillus subtilis] sp|P37528|YAAE_BACSU Hypothetical UPF0030 protein yaaE pir||S66042 conserved hypothetical protein yaaE - Bacillus subtilis dbj|BAA05248.1| unknown [Bacillus subtilis] emb|CAB11788.1| yaaE [Bacillus subtilis subsp. subtilis str. 168] Length = 196 Score = 196 bits (497), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 97/187 (51%), Positives = 127/187 (67%) Query: 1 MKIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGF 60 + IGVL LQGAV EHI +IE GA GL +KR STT+ +L+ Y F Sbjct: 2 LTIGVLGLQGAVREHIHAIEACGAAGLVVKRPEQLNEVDGLILPGGESTTMRRLIDTYQF 61 Query: 61 IEAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLD 120 +E +R+FA +GKP+FGTCAG+I+LAK I G + HL L+++ V RN+FGRQ +SFE DL Sbjct: 62 MEPLREFAAQGKPMFGTCAGLIILAKEIAGSDNPHLGLLNVVVERNSFGRQVDSFEADLT 121 Query: 121 VKGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLHQ 180 +KG+DEP VFIRAP I E G +V+VL+ + IV A+QG L SFHPELT+D+R+ Q Sbjct: 122 IKGLDEPFTGVFIRAPHILEAGENVEVLSEHNGRIVAAKQGQFLGCSFHPELTEDHRVTQ 181 Query: 181 YFADMVE 187 F +MVE Sbjct: 182 LFVEMVE 188 >ref|NP_829920.1| pyridoxine biosynthesis amidotransferase [Bacillus cereus ATCC 14579] gb|AAP07121.1| pyridoxine biosynthesis amidotransferase [Bacillus cereus ATCC 14579] Length = 196 Score = 193 bits (491), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 95/189 (50%), Positives = 128/189 (67%) Query: 1 MKIGVLALQGAVTEHIVSIEKTGAQGLPIKRAXXXXXXXXXXXXXXXSTTIGKLMRKYGF 60 +KIGVL LQGAV EH+ S+E +GA+ + +KR STT+ +L+ KY F Sbjct: 2 VKIGVLGLQGAVREHVKSVEASGAEAVVVKRIEQLEEIDGLILPGGESTTMRRLIDKYAF 61 Query: 61 IEAIRDFAGRGKPVFGTCAGMIVLAKRIDGGEPAHLELMDITVARNAFGRQRESFECDLD 120 +E +R FA GKP+FGTCAGMI+LAK + G E AH+ MDITV RNAFGRQ++SFE L Sbjct: 62 MEPLRTFAKSGKPMFGTCAGMILLAKTLIGYEEAHIGAMDITVERNAFGRQKDSFEAALS 121 Query: 121 VKGIDEPVRAVFIRAPLITEVGPDVDVLTVYKDEIVTARQGNLLVSSFHPELTDDYRLHQ 180 ++G+ E VFIRAP + EV +V+VL+ + D +V RQ L +SFHPELTDD+R+ Sbjct: 122 IEGVGEDFVGVFIRAPYVVEVADNVEVLSKHGDRMVAVRQNQFLAASFHPELTDDHRVTA 181 Query: 181 YFADMVEHS 189 YF +MV+ + Sbjct: 182 YFVEMVKEA 190 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 249,172,190 Number of extensions: 8995083 Number of successful extensions: 17996 Number of sequences better than 1.0e-05: 112 Number of HSP's gapped: 17749 Number of HSP's successfully gapped: 112 Length of query: 194 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 75 Effective length of database: 434,140,797 Effective search space: 32560559775 Effective search space used: 32560559775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 122 (51.6 bits)