BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0013.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAG28533.1| cysteine synthase [Geobacillus stearothermophilus] 410 e-113 ref|NP_240954.1| cysteine synthase A [Bacillus halodurans] >gi|2... 404 e-111 dbj|BAC55275.1| O-acetyl-L-serine sulfhydrylase [Geobacillus ste... 399 e-110 ref|NP_976394.1| cysteine synthase A [Bacillus cereus ATCC 10987... 398 e-109 ref|NP_387954.1| cysteine synthetase A [Bacillus subtilis] >gi|5... 396 e-109 >gb|AAG28533.1| cysteine synthase [Geobacillus stearothermophilus] Length = 308 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 213/311 (68%), Positives = 258/311 (82%), Gaps = 4/311 (1%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MA+ VNS+T+LIG TP V+LNR+V E SA++YLKLE+ NPGSSVKDRIA++++E AEKEG Sbjct: 1 MARTVNSITELIGDTPAVKLNRIVDEDSADVYLKLEFMNPGSSVKDRIALAMIEAAEKEG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LKPG TI+E TSGNTGIGLA+VAAAKGYKA++VMP+TMSLERRNLLRAYGAELVLTPGS Sbjct: 61 KLKPGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPDTMSLERRNLLRAYGAELVLTPGS 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 +GM GA++KAE+++ E+ YF+ +QFKN+AN +IHR TTG EIV E +G LDAF Sbjct: 121 QGMRGAIEKAEELVREH-GYFMPQQFKNEANPEIHRLTTGKEIV---EQMGDQLDAFIAG 176 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 +VL+ KYPN+KIYAVEPA SP+L+GGKPGPHKIQGIGA F+PD+L+ N+ Sbjct: 177 VGTGGTITGAGQVLREKYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDVLDTNI 236 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDE+I V +EAF ARR A+EEG+L GISSGAA+ AALKVAK+LG GKKV+ IIPSNGE Sbjct: 237 YDEVITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKQLGKGKKVLAIIPSNGE 296 Query: 301 RYLSTPLYNFE 311 RYLSTPLY FE Sbjct: 297 RYLSTPLYQFE 307 >ref|NP_240954.1| cysteine synthase A [Bacillus halodurans] pir||H83660 cysteine synthase (EC 4.2.99.8) [similarity] - Bacillus halodurans (strain C-125) dbj|BAB03807.1| cysteine synthase A [Bacillus halodurans] Length = 307 Score = 404 bits (1039), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/309 (64%), Positives = 246/309 (79%), Gaps = 4/309 (1%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEGLL 62 +V NS+TDLIG TPLV+LNR+V + A++YLKLE+ NPGSSVKDRIA++++E AEK G L Sbjct: 2 RVANSITDLIGQTPLVKLNRLVSDKHADVYLKLEFFNPGSSVKDRIALAMIEAAEKSGAL 61 Query: 63 KPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGSEG 122 KP TIVE TSGNTGIGLA+VAAAKGYK +VMPETMSLERRNLLRAYGAELVLTPG EG Sbjct: 62 KPDDTIVEPTSGNTGIGLAMVAAAKGYKTKLVMPETMSLERRNLLRAYGAELVLTPGPEG 121 Query: 123 MNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXXXX 182 M GA++KA ++ EN YF+ +QF+N+AN +IHRETTG E++E ++ G LD F Sbjct: 122 MGGAIRKATELAQEN-GYFMPQQFENEANPEIHRETTGRELLEQVD---GQLDGFVSGIG 177 Query: 183 XXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNVYD 242 +LK +PN+ I AVEPA SP+L+GG PGPHKIQGIGA F+P IL+ ++YD Sbjct: 178 TGGTITGAGGLLKENFPNLHIAAVEPADSPVLSGGNPGPHKIQGIGAGFVPKILDTDIYD 237 Query: 243 EIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGERY 302 E+I V ++AFE ARR AKEEG+L GISSGAA++AAL+VA++LG GKKVV +IPSNGERY Sbjct: 238 EVIKVSTEQAFEYARRAAKEEGILGGISSGAAIYAALQVAEKLGPGKKVVAVIPSNGERY 297 Query: 303 LSTPLYNFE 311 LSTPLY FE Sbjct: 298 LSTPLYQFE 306 >dbj|BAC55275.1| O-acetyl-L-serine sulfhydrylase [Geobacillus stearothermophilus] Length = 308 Score = 399 bits (1026), Expect = e-110, Method: Compositional matrix adjust. Identities = 211/311 (67%), Positives = 253/311 (81%), Gaps = 4/311 (1%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 MA+ VNS+T+LIG TP V+LNR+V E SA++YLKLE+ NPGSSVKDRIA++++E AEK G Sbjct: 1 MARTVNSITELIGDTPAVKLNRIVDEDSADVYLKLEFMNPGSSVKDRIALAMIEAAEKAG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LKPG TIVE TSGNTGIGLA+VAAAKGYKAV+VMP+TMSLERRNLLRAYGAELVLTPG+ Sbjct: 61 KLKPGDTIVEPTSGNTGIGLAMVAAAKGYKAVLVMPDTMSLERRNLLRAYGAELVLTPGA 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 +GM G + KAE+++ E+ YF+ +QFKN+AN +IHR TTG EIV E +G LDAF Sbjct: 121 QGMRGPIAKAEELVREH-GYFMPQQFKNEANPEIHRLTTGKEIV---EQMGDQLDAFVAG 176 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 +VL+ YPN+KIYAVEPA SP+L+GGKPGPHKIQGIGA F+PDIL+ ++ Sbjct: 177 VGTGGTTTGAGKVLREAYPNIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPDILDTSI 236 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YD +I V +EAF ARR A+EEG+L GISSGAA+ AALKVAKELG GKKV+ IIPSNGE Sbjct: 237 YDGVITVTTEEAFAAARRAAREEGILGGISSGAAIHAALKVAKELGKGKKVLAIIPSNGE 296 Query: 301 RYLSTPLYNFE 311 RYLSTPLY FE Sbjct: 297 RYLSTPLYQFE 307 >ref|NP_976394.1| cysteine synthase A [Bacillus cereus ATCC 10987] gb|AAS39002.1| cysteine synthase A [Bacillus cereus ATCC 10987] Length = 307 Score = 398 bits (1022), Expect = e-109, Method: Compositional matrix adjust. Identities = 207/309 (66%), Positives = 253/309 (81%), Gaps = 4/309 (1%) Query: 3 KVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEGLL 62 +V SV++LIG TP+V+LNR+V SA+IYLKLE+ NPGSSVKDRIA++++E+AEK+GLL Sbjct: 2 RVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLL 61 Query: 63 KPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGSEG 122 K G TI+E TSGNTGIGLA+VAAAKGYKA++VMPETMS+ERRNLLRAYGAELVLTPG EG Sbjct: 62 KEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSIERRNLLRAYGAELVLTPGPEG 121 Query: 123 MNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXXXX 182 M GA++KA ++ EN YF+ +QFKN++N +IHR TTGPEIV E +G LDAF Sbjct: 122 MGGAIRKATELAKEN-GYFIPQQFKNQSNPEIHRLTTGPEIV---EQMGDQLDAFIAGIG 177 Query: 183 XXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNVYD 242 EVLK Y ++KIYAVEPA SP+L+GGKPGPHKIQGIGA FIP+ L+ VYD Sbjct: 178 TGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFIPETLDVEVYD 237 Query: 243 EIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGERY 302 EI+ V+ ++AFE ARRVAKEEG+L GISSGA ++AA ++AK+LG GKKV+VIIPSNGERY Sbjct: 238 EIVQVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEIAKKLGKGKKVLVIIPSNGERY 297 Query: 303 LSTPLYNFE 311 LSTPLY FE Sbjct: 298 LSTPLYQFE 306 >ref|NP_387954.1| cysteine synthetase A [Bacillus subtilis] sp|P37887|CYSK_BACSU Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) (CSase) (Superoxide-inducible protein 11) (SOI11) pir||S66103 cysteine synthase (EC 4.2.99.8) A - Bacillus subtilis dbj|BAA05308.1| cysteine synthetase A [Bacillus subtilis] emb|CAB11849.1| cysteine synthetase A [Bacillus subtilis subsp. subtilis str. 168] Length = 308 Score = 396 bits (1018), Expect = e-109, Method: Compositional matrix adjust. Identities = 208/311 (66%), Positives = 251/311 (80%), Gaps = 3/311 (0%) Query: 1 MAKVVNSVTDLIGGTPLVRLNRVVPEGSAEIYLKLEYQNPGSSVKDRIAISIVEEAEKEG 60 M +V NS+T+LIG TP+V+LNR+ E SA++YLKLEY NPGSSVKDRI ++++E AEKEG Sbjct: 1 MVRVANSITELIGNTPIVKLNRLADENSADVYLKLEYMNPGSSVKDRIGLAMIEAAEKEG 60 Query: 61 LLKPGGTIVEATSGNTGIGLALVAAAKGYKAVIVMPETMSLERRNLLRAYGAELVLTPGS 120 LK G TI+E TSGNTGIGLA+VAAAKG KA++VMP+TMS+ERRNLLRAYGAELVLTPG+ Sbjct: 61 KLKAGNTIIEPTSGNTGIGLAMVAAAKGLKAILVMPDTMSMERRNLLRAYGAELVLTPGA 120 Query: 121 EGMNGAVKKAEQILSENPDYFLAEQFKNKANVKIHRETTGPEIVEAIESIGGPLDAFXXX 180 EGM GA+KKAE+ L+E YF+ +QF N +N +IHR+TTG EIVE LDAF Sbjct: 121 EGMKGAIKKAEE-LAEKHGYFVPQQFNNPSNPEIHRQTTGKEIVEQFGD--DQLDAFVAG 177 Query: 181 XXXXXXXXXXXEVLKSKYPNVKIYAVEPAASPILAGGKPGPHKIQGIGANFIPDILNQNV 240 EVLK YP++KIYAVEP+ SP+L+GGKPGPHKIQGIGA F+PDILN V Sbjct: 178 IGTGGTITGAGEVLKEAYPSIKIYAVEPSDSPVLSGGKPGPHKIQGIGAGFVPDILNTEV 237 Query: 241 YDEIIHVENDEAFETARRVAKEEGVLSGISSGAAVFAALKVAKELGAGKKVVVIIPSNGE 300 YDEI V+N+EAFE ARR A+EEG+L GISSGAA++AAL+VAK+LG GKKV+ IIPSNGE Sbjct: 238 YDEIFPVKNEEAFEYARRAAREEGILGGISSGAAIYAALQVAKKLGKGKKVLAIIPSNGE 297 Query: 301 RYLSTPLYNFE 311 RYLSTPLY F+ Sbjct: 298 RYLSTPLYQFD 308 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 451,045,127 Number of extensions: 18069761 Number of successful extensions: 52159 Number of sequences better than 1.0e-05: 1215 Number of HSP's gapped: 50193 Number of HSP's successfully gapped: 1223 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 126 (53.1 bits)