BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0060.0004.p (484 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S66097 cell-cycle protein homolog yacA - Bacillus subtilis ... 211 5e-53 ref|NP_387948.1| similar to cell-cycle protein [Bacillus subtili... 207 4e-52 ref|NP_463750.1| fusion protein, N-terminal part similar to B. s... 201 2e-50 gb|AAM74001.1| YacA/HprT-like protein [Listeria monocytogenes] 201 3e-50 ref|NP_654012.1| UPF0021, Uncharacterized protein family UPF0021... 194 4e-48 >pir||S66097 cell-cycle protein homolog yacA - Bacillus subtilis dbj|BAA05302.1| unknown [Bacillus subtilis] Length = 486 Score = 211 bits (536), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 139/455 (30%), Positives = 227/455 (49%), Gaps = 12/455 (2%) Query: 5 KELVESVLESAAEHGLWSPHDTIVVAVSGGPDSVALLHILHEISLNRMPLTLICAHVNHG 64 +++V+SV + +H L TI+V VSGGPDS+ALLH LH +L +I AHV+H Sbjct: 12 EDIVKSVKDFLNKHNLTLKGATIIVGVSGGPDSMALLHALH--TLCGRSANVIAAHVDHR 69 Query: 65 FR-PESVEEAEAVRAMAGRLGLPFELAEFDIPSLVKESGLGPEGAAREKRYQFLIDTAHR 123 FR ES E+ V+A L E A+ ++ + +E GL + AAR+ RYQF + + Sbjct: 70 FRGAESEEDMRFVQAYCKAEQLVCETAQINVTAYAQEKGLNKQAAARDCRYQFFEEIMSK 129 Query: 124 YAARSVALAHHADDQAETVXXXXXXXXXXXXXXXXXWKRTEKKVELIRPFLRINKTALVS 183 + A +ALAHH DDQ ET+ R +IRPFL I K ++ Sbjct: 130 HQADYLALAHHGDDQVETMLMKLAKGTLGTGLAGMQPVRRFGTGRIIRPFLTITKEEILH 189 Query: 184 LCRQEGLPYAEDASNLMTEYRRNAVRLNLIPALEAYNPKVRQSLLQLSEIVQAEDEYMEA 243 C + GL Y D SN +Y RN R ++P L+ +P V + ++SE + +++++++ Sbjct: 190 YCHENGLSYRTDESNAKDDYTRNRFRKTVLPFLKQESPDVHKRFQKVSEALTEDEQFLQS 249 Query: 244 AAQKYFNELVLADAGKCT-MQRALFTAIPSALQRRLIKLILNYLSAD-SSSLDFPKIEAV 301 + N+++ + + + + A+P LQRR ++LILNYL + SS I+ Sbjct: 250 LTKDEMNKVITSQSNTSVEINSSQLLALPMPLQRRGVQLILNYLYENVPSSFSAHHIQQF 309 Query: 302 RRGTLQDYPTVWSLNMGGGLICMRQYDTIVFSSKPPQ-QESSYTYRLSLPHPRVEIKEIG 360 P+ L+ GL ++ Y T +F+ + Q + + Y++S + G Sbjct: 310 LDWAENGGPS-GVLDFPKGLKVVKSYQTCLFTFEQWQCKNVPFEYQISGAADETAVLPNG 368 Query: 361 KVLTMSVLERENFTVLGEDSGKMLAWFDSGELVLPLTIRSRLPGDTIRVMGLNGSXXXXX 420 ++E ++ E+ G + ++ PLTIR+R GD I++ G+NGS Sbjct: 369 -----YLIEARHYADSPEEHGNAVFITSEKKVRFPLTIRTRKAGDRIKLKGMNGSKKVKD 423 Query: 421 XXXXXXXPSSERAAIPLVCDDLGNIVWIPGVRRSM 455 P ER P+V D G I+WIPG+++S+ Sbjct: 424 IFIDKKLPLQERDNWPIVTDASGEIIWIPGLKKSI 458 >ref|NP_387948.1| similar to cell-cycle protein [Bacillus subtilis] sp|P37563|YACA_BACSU Hypothetical protein yacA emb|CAB11843.1| yacA [Bacillus subtilis subsp. subtilis str. 168] Length = 472 Score = 207 bits (528), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 138/452 (30%), Positives = 224/452 (49%), Gaps = 12/452 (2%) Query: 8 VESVLESAAEHGLWSPHDTIVVAVSGGPDSVALLHILHEISLNRMPLTLICAHVNHGFR- 66 ++SV + +H L TI+V VSGGPDS+ALLH LH +L +I AHV+H FR Sbjct: 1 MKSVKDFLNKHNLTLKGATIIVGVSGGPDSMALLHALH--TLCGRSANVIAAHVDHRFRG 58 Query: 67 PESVEEAEAVRAMAGRLGLPFELAEFDIPSLVKESGLGPEGAAREKRYQFLIDTAHRYAA 126 ES E+ V+A L E A+ ++ + +E GL + AAR+ RYQF + ++ A Sbjct: 59 AESEEDMRFVQAYCKAEQLVCETAQINVTAYAQEKGLNKQAAARDCRYQFFEEIMSKHQA 118 Query: 127 RSVALAHHADDQAETVXXXXXXXXXXXXXXXXXWKRTEKKVELIRPFLRINKTALVSLCR 186 +ALAHH DDQ ET+ R +IRPFL I K ++ C Sbjct: 119 DYLALAHHGDDQVETMLMKLAKGTLGTGLAGMQPVRRFGTGRIIRPFLTITKEEILHYCH 178 Query: 187 QEGLPYAEDASNLMTEYRRNAVRLNLIPALEAYNPKVRQSLLQLSEIVQAEDEYMEAAAQ 246 + GL Y D SN +Y RN R ++P L+ +P V + ++SE + +++++++ + Sbjct: 179 ENGLSYRTDESNAKDDYTRNRFRKTVLPFLKQESPDVHKRFQKVSEALTEDEQFLQSLTK 238 Query: 247 KYFNELVLADAGKCT-MQRALFTAIPSALQRRLIKLILNYLSAD-SSSLDFPKIEAVRRG 304 N+++ + + + + A+P LQRR ++LILNYL + SS I+ Sbjct: 239 DEMNKVITSQSNTSVEINSSQLLALPMPLQRRGVQLILNYLYENVPSSFSAHHIQQFLDW 298 Query: 305 TLQDYPTVWSLNMGGGLICMRQYDTIVFSSKPPQ-QESSYTYRLSLPHPRVEIKEIGKVL 363 P+ L+ GL ++ Y T +F+ + Q + + Y++S + G Sbjct: 299 AENGGPS-GVLDFPKGLKVVKSYQTCLFTFEQWQCKNVPFEYQISGAADETAVLPNG--- 354 Query: 364 TMSVLERENFTVLGEDSGKMLAWFDSGELVLPLTIRSRLPGDTIRVMGLNGSXXXXXXXX 423 ++E ++ E+ G + ++ PLTIR+R GD I++ G+NGS Sbjct: 355 --YLIEARHYADSPEEHGNAVFITSEKKVRFPLTIRTRKAGDRIKLKGMNGSKKVKDIFI 412 Query: 424 XXXXPSSERAAIPLVCDDLGNIVWIPGVRRSM 455 P ER P+V D G I+WIPG+++S+ Sbjct: 413 DKKLPLQERDNWPIVTDASGEIIWIPGLKKSI 444 >ref|NP_463750.1| fusion protein, N-terminal part similar to B. subtilis YacA protein, C-terminal part similar to hypoxanthine-guanine phosphoribosyltransferase [Listeria monocytogenes EGD-e] pir||AD1102 hypoxanthine-guanine phosphoribosyltransferase homolog lmo0219 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00746.1| fusion protein, N-terminal part similar to B. subtilis YacA protein, C-terminal part similar to hypoxanthine-guanine phosphoribosyltransferase [Listeria monocytogenes] Length = 648 Score = 201 bits (512), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 142/458 (31%), Positives = 217/458 (47%), Gaps = 18/458 (3%) Query: 4 MKELVESVLESAAEHGLWSPHDTIVVAVSGGPDSVALLHILHEISLNRMPLTLI-CAHVN 62 M + + V + +H L D ++VAVSGGPDS+ALLH L +L +P I AH+N Sbjct: 1 MDDTEKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNL--VPKEAISVAHLN 58 Query: 63 HGFRPESVEEAEAVRAMAGRLGLPFELAEFDIPSLVKESGLGPEGAAREKRYQFLIDTAH 122 H R + +E V R G+PF + E DI S + G E AR RY F Sbjct: 59 HQLRENAAKEQRVVETFCERQGIPFYIEEVDIKSRAQSLQKGLEETARIVRYDFFEKVMA 118 Query: 123 RYAARSVALAHHADDQAETVXXXXXXXXXXXXXXXXXWKRTEKKVELIRPFLRINKTALV 182 + LAHHADDQ ET+ KR K + IRPFL I K ++ Sbjct: 119 EKNINKLVLAHHADDQIETILMRLVRGSASIGWSGIQPKRELKGGQAIRPFLPITKAEII 178 Query: 183 SLCRQEGLPYAEDASNLMTEYRRNAVRLNLIPALEAYNPKVRQSLLQLSEIVQAEDEYME 242 ++ L Y D SN EY RN R L+P L+ NP V + SE + +++E Sbjct: 179 DYAQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLE 238 Query: 243 AAAQKYFNELVLADAGKCTMQRALFTAIPSALQRRLIKLILNYL-SADSSSLDFPKIEAV 301 A A + ++ + + T+ + F + LQRR I L+L YL + D+S + I + Sbjct: 239 ALASDLLKKNLIKNGKQTTLLLSSFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQI 298 Query: 302 RRGTLQDYPTVWSLNMGGGLICMRQYDTIVFSSKPPQQESSYTYRLSLPHPRVEIKEIGK 361 + D P+ S+++ LI R YD + F + S + ++L L + R+E+ Sbjct: 299 IQMIQSDNPSS-SIDLPNKLIANRAYDKLHFQFGEREAPSEFYHQLEL-NDRIELDNKAS 356 Query: 362 V---LTMSVLERENFTVLGEDSGKMLAWFDSGELVLPLTIRSRLPGDTIRVMGLNGSXXX 418 + L SV++ +G +L D+ E+ LPL +R+R+ GD + + G GS Sbjct: 357 IRLKLKSSVVQTNGL------NGMLL---DAEEITLPLIVRNRVNGDRMTMKGQAGSKKL 407 Query: 419 XXXXXXXXXPSSERAAIPLVCDDLGNIVWIPGVRRSMH 456 P ER +P++ D G I+W+PGV++S + Sbjct: 408 KDIFIDAKIPRQERDKLPVITDYTGKILWVPGVKKSAY 445 >gb|AAM74001.1| YacA/HprT-like protein [Listeria monocytogenes] Length = 648 Score = 201 bits (511), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 141/458 (30%), Positives = 216/458 (47%), Gaps = 18/458 (3%) Query: 4 MKELVESVLESAAEHGLWSPHDTIVVAVSGGPDSVALLHILHEISLNRMPLTLI-CAHVN 62 M + + V + +H L D ++VAVSGGPDS+ALLH L +L +P I AH+N Sbjct: 1 MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNL--VPKEAISVAHIN 58 Query: 63 HGFRPESVEEAEAVRAMAGRLGLPFELAEFDIPSLVKESGLGPEGAAREKRYQFLIDTAH 122 H R + E V A G+PF + E D+ S + G E AR RY F Sbjct: 59 HHLRENAANEQNVVEAFCESQGIPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMA 118 Query: 123 RYAARSVALAHHADDQAETVXXXXXXXXXXXXXXXXXWKRTEKKVELIRPFLRINKTALV 182 +ALAHHADDQ ET+ KR K + IRPFL I K ++ Sbjct: 119 EKKINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKRELKGGQAIRPFLPITKAEII 178 Query: 183 SLCRQEGLPYAEDASNLMTEYRRNAVRLNLIPALEAYNPKVRQSLLQLSEIVQAEDEYME 242 ++ L Y D SN EY RN R L+P L+ NP V + SE + +++E Sbjct: 179 DYAQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLE 238 Query: 243 AAAQKYFNELVLADAGKCTMQRALFTAIPSALQRRLIKLILNYL-SADSSSLDFPKIEAV 301 A A + ++ + + T+ F + LQRR I L+L YL + D+S + I + Sbjct: 239 ALASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQI 298 Query: 302 RRGTLQDYPTVWSLNMGGGLICMRQYDTIVFSSKPPQQESSYTYRLSLPHPRVEIKEIGK 361 + D P+ S+++ L+ R YD + F + S + ++L L + R+E+ Sbjct: 299 IQMIQSDNPSS-SIDLPNKLVANRAYDKLHFQFGEREAPSEFYHQLEL-NDRIELDNKAS 356 Query: 362 V---LTMSVLERENFTVLGEDSGKMLAWFDSGELVLPLTIRSRLPGDTIRVMGLNGSXXX 418 + L SV++ +G +L D+ E+ LPL +R+R+ GD + + G GS Sbjct: 357 IRLKLKSSVVQTNGL------NGMLL---DAEEITLPLIVRNRVNGDRMTMKGQAGSKKL 407 Query: 419 XXXXXXXXXPSSERAAIPLVCDDLGNIVWIPGVRRSMH 456 P ER +P++ D G I+W+PGV++S + Sbjct: 408 KDIFIDAKIPRQERDKLPVITDYTGKILWVPGVKKSAY 445 >ref|NP_654012.1| UPF0021, Uncharacterized protein family UPF0021 [Bacillus anthracis A2012] ref|NP_842631.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP24117.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 476 Score = 194 bits (493), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 145/462 (31%), Positives = 218/462 (47%), Gaps = 15/462 (3%) Query: 7 LVESVLESAAEHGLWSPHDTIVVAVSGGPDSVALLHILHEISLNRMPLTLICAHVNHGFR 66 VE V + +H + TIVV VSGGPDS+ALL+ L E + ++ AHV+H FR Sbjct: 5 FVEKVDDFVRQHDVLKERSTIVVGVSGGPDSLALLYYLLEKRAAKQ-FEIVVAHVDHMFR 63 Query: 67 -PESVEEAEAVRAMAGRLGLPFELAEFDIPSLVKESGLGPEGAAREKRYQFLIDTAHRYA 125 ES E+ + V+ + LG+ E ++ K+ G+ + AARE RY FL +Y Sbjct: 64 GDESHEDLQFVQDLCKGLGVICETIRINVSQYQKQYGMNAQVAARECRYAFLERIMKKYD 123 Query: 126 ARSVALAHHADDQAETVXXXXXXXXXXXXXXXXXWKRTEKKVELIRPFLRINKTALVSLC 185 AR VAL HH DDQ ET+ KR LIRP L + K +V+ C Sbjct: 124 ARYVALGHHGDDQVETILMRLVRGSTPKGYAGIAVKRPFHNGYLIRPLLGVTKEEIVNYC 183 Query: 186 RQEGLPYAEDASNLMTEYRRNAVRLNLIPALEAYNPKVRQSLLQLSEIVQAEDEYMEAAA 245 + + D SN Y RN +R ++P L+ NP+V + + S +Q ++ Y++ A Sbjct: 184 NELKIIPRIDPSNKKEVYTRNRLRKYVLPHLKEENPQVHEKFQKFSMQMQEDEAYLQELA 243 Query: 246 QKYFNELVLADAGK-CTMQRALFTAIPSALQRRLIKLILNYLSADS--SSLDFPKIEAVR 302 + N+++ + K ++ F ++ LQRR I+LILNYL SSL I+ V Sbjct: 244 FEKMNKVITKKSDKQISLSIPTFESMSMPLQRRGIQLILNYLYEYKIPSSLSSIHIDKVI 303 Query: 303 RGTLQDYPTVWSLNMGGGLICMRQYDTIVFSSKPPQQESSYTYRLSLPHPRVEIKEIGKV 362 + P+ SL+ G L +R Y+ F K QE + L P G Sbjct: 304 EFFKRTQPS-GSLDFPGDLKIVRAYEECSFGFK---QEIVSPFLQDLSVPGTITLSNGDK 359 Query: 363 LTMSVLERENFTVLGEDSGKMLAWFDSGELVLPLTIRSRLPGDTIRVMGLNGSXXXXXXX 422 L V E + D + + ++ P+ IRSR GD + + G+NG+ Sbjct: 360 LVTEVSED-----IPSDMNETVFVAKYNDISYPIRIRSRENGDRMSIQGMNGTKKIKAIF 414 Query: 423 XXXXXPSSERAAIPLVCDDLGNIVWIPGVRRSMHAAVGRHTA 464 P +R P+VCD GNI+W+P ++RS A+ + TA Sbjct: 415 IEAKVPREKREEWPVVCDASGNIIWLPLLKRSAF-AISKETA 455 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 626,258,176 Number of extensions: 23231141 Number of successful extensions: 59976 Number of sequences better than 1.0e-05: 388 Number of HSP's gapped: 59476 Number of HSP's successfully gapped: 389 Length of query: 484 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 354 Effective length of database: 404,274,708 Effective search space: 143113246632 Effective search space used: 143113246632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)